高级创伤生命支持(advanced trauma life support,ATLS)培训自1978年在美国首次开展以来,已被认为是创伤救治培训的规范教程和创伤救治方法的指南,被世界广大医务人员所接受、推广。该教程由美国外科医师协会(ACS)下属的创伤委...高级创伤生命支持(advanced trauma life support,ATLS)培训自1978年在美国首次开展以来,已被认为是创伤救治培训的规范教程和创伤救治方法的指南,被世界广大医务人员所接受、推广。该教程由美国外科医师协会(ACS)下属的创伤委员会(COT)、ATLS国际会员、美国急诊医师联合会(ACEP)等联合编写,具有知识产权和商标,任何没有经过ACS批准的个人和组织不能使用该教程培训,因此,我国很少有医务人员接受ATIS培训,亦未曾见过ATLS培训效果报道。展开更多
The Arabidopsis Toxicos en Levadura(ATL)protein is a subfamily of the E3 ubiquitin ligases,which exists widely in plants and is extensively involved in plant growth and development.Although the ATL family has been ide...The Arabidopsis Toxicos en Levadura(ATL)protein is a subfamily of the E3 ubiquitin ligases,which exists widely in plants and is extensively involved in plant growth and development.Although the ATL family has been identified in other species,such as Arabidopsis,Oryza sativa,and grapevine,few reports on pear ATL gene families have been reported.In this study,92 PbrATL genes were identified and analyzed from the Pyrus breschneideri genome.Motif analysis and phylogenetic tree generation divided them into nine subgroups,and chromosome localization analysis showed that the 92 PbrATL genes were distributed in 16 of 17 pear chromosomes.Transcriptome data and quantitative real-time polymerase chain reaction(qRT-PCR)experiments demonstrated that PbrATL18,PbrATL41,and PbrATL88 were involved in both pear drought resistance and Colletotrichum fructicola infection.In addition,Arabidopsis thaliana overexpressing PbrATL18 showed greater resistance to drought stress than the wild type(WT),and PbrATL18-silenced pear seedlings showed greater sensitivity to drought and C.fructicola infection than the controls.PbrATL18 regulated plant resistance by regulating chitinase(CHI),phenylalanine ammonia-lyase(PAL),polyphenol oxidase(PPO),catalase(CAT),peroxidase(POD),and superoxide dismutase(SOD)activities.This study provided a reference for further exploring the functions of the PbrATL gene in drought resistance and C.fructicola infection.展开更多
文摘高级创伤生命支持(advanced trauma life support,ATLS)培训自1978年在美国首次开展以来,已被认为是创伤救治培训的规范教程和创伤救治方法的指南,被世界广大医务人员所接受、推广。该教程由美国外科医师协会(ACS)下属的创伤委员会(COT)、ATLS国际会员、美国急诊医师联合会(ACEP)等联合编写,具有知识产权和商标,任何没有经过ACS批准的个人和组织不能使用该教程培训,因此,我国很少有医务人员接受ATIS培训,亦未曾见过ATLS培训效果报道。
基金supported by the National Key Research and Development Program of China(Grant No.2022YFD1200503)Jiangsu Agriculture Science and Technology Innovation Fund[Grant No.CX(22)3046]+2 种基金the National Science Foundation of China(Grant No.32072538)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutionsthe Earmarked Fund for China Agriculture Research System(Grant No.CARS-28).
文摘The Arabidopsis Toxicos en Levadura(ATL)protein is a subfamily of the E3 ubiquitin ligases,which exists widely in plants and is extensively involved in plant growth and development.Although the ATL family has been identified in other species,such as Arabidopsis,Oryza sativa,and grapevine,few reports on pear ATL gene families have been reported.In this study,92 PbrATL genes were identified and analyzed from the Pyrus breschneideri genome.Motif analysis and phylogenetic tree generation divided them into nine subgroups,and chromosome localization analysis showed that the 92 PbrATL genes were distributed in 16 of 17 pear chromosomes.Transcriptome data and quantitative real-time polymerase chain reaction(qRT-PCR)experiments demonstrated that PbrATL18,PbrATL41,and PbrATL88 were involved in both pear drought resistance and Colletotrichum fructicola infection.In addition,Arabidopsis thaliana overexpressing PbrATL18 showed greater resistance to drought stress than the wild type(WT),and PbrATL18-silenced pear seedlings showed greater sensitivity to drought and C.fructicola infection than the controls.PbrATL18 regulated plant resistance by regulating chitinase(CHI),phenylalanine ammonia-lyase(PAL),polyphenol oxidase(PPO),catalase(CAT),peroxidase(POD),and superoxide dismutase(SOD)activities.This study provided a reference for further exploring the functions of the PbrATL gene in drought resistance and C.fructicola infection.