To our knowledge,no single study has systemically compared cryopreservation efficiencies of bovine blastocysts derived from in vitro fertilization （IVF）,intracytoplasmic sperm injection （ICSI） and somatic cell nuc...To our knowledge,no single study has systemically compared cryopreservation efficiencies of bovine blastocysts derived from in vitro fertilization （IVF）,intracytoplasmic sperm injection （ICSI） and somatic cell nuclear transfer （SCNT） by controlled freezing and vitrification.This experiment,therefore,was designed to compare the cryopreservation of these blastocysts with controlled freezing and OPS vitrification.Adenosine-5’-triphosphate （ATP） content and reactive oxygen species （ROS） level in blastocysts were also analyzed.Firstly,for each type of blastocyst （IVF,ICSI or SCNT）,significant differences were observed between the survival rates of the controlled freezing （（81.56±2.33）,（68.18±4.72） or （47.89±5.83）%） and OPS vitrification groups （（92.24±4.54）,（82.40±3.76） or （78.71±5.91）%;P〈0.05）.Secondly,for each type of blastocyst （IVF,ICSI or SCNT）,ATP content was significantly decreased after controlled freezing or vitrification,and the ATP content in the controlled freezing group （0.43±0.06）,（0.35±0.05） or （0.21±0.02） pmol） was significantly lower than that found in the OPS vitrification group （0.62±0.04）,（0.46±0.03） or （0.30±0.01） pmol;P〈0.05）.Thirdly,ROS level in fresh IVF （（47.33±3.56） c.p.s （counted photons per second）,ICSI （（36.51±2.58） c.p.s） or SCNT blastocysts （（26.44±1.49） c.p.s） was significantly lower than that found in the OPS vitrification group （（72.14±4.31）,（58.89±3.89） or （40.11±5.73） c.p.s;P〈0.05）,but higher than that of the controlled freezing group （34.41±3.32）,（23.13±1.26） or （15.46±2.45） c.p.s;P〈0.05）.The present study indicated that vitrification is more efficient in the cryopreservation of bovine blastocysts derived from IVF,ICSI or SCNT than controlled freezing.Furthermore,both vitrification and controlled freezing significantly altered the ATP content and ROS level in those blastocysts.展开更多
To investigate the relationship between the ATP content in human oocytes and the deve-lopmental potential of human oocytes, unfertilized oocytes from clinical IVF and immature oocytes unsuitable for ICSI were collecte...To investigate the relationship between the ATP content in human oocytes and the deve-lopmental potential of human oocytes, unfertilized oocytes from clinical IVF and immature oocytes unsuitable for ICSI were collected. The ATP content of these unfertilized and in vitro matured eggs were determined quantitatively by measuring the luminescence using an ATP-dependent bioluminescence assay. The result showed that the ATP content of unfertilized oocytes was higher than in vitro matured ones (2.20±0.67 pmol vs 1.72±0.49 pmol, P<0.05 ). In unfertilized oocytes, the ATP content of those whose fertilization rates (FR) was higher than 50 % was 2.43±0.60 pmol, which was significantly different from those whose FR was less than 50 % (1.72±0.56 pmol), while in in vitro matured oocytes, the ATP content of those whose FR more than 50 % was 1.8±0.44 pmol, slightly higher than those less than 50 % (1.55±0.40 pmol), without statistical significance. There was a tendency that the ATP content of oocytes of pregnant patients was higher than those of controls, but the sample number was too small to show any significance in statistics. Briefly, there was positive correlation between the ATP content in oocytes and developmental potential of oocytes.展开更多
This study demonstrates a concentration dependent inhibition of carbon fixation, O2 evolution, photosynthetic electron transport chain and ATP content of A. doliolum and C. vulgaris by Cu, Ni and Fe. Although the mode...This study demonstrates a concentration dependent inhibition of carbon fixation, O2 evolution, photosynthetic electron transport chain and ATP content of A. doliolum and C. vulgaris by Cu, Ni and Fe. Although the mode of inhibition of photosynthetic electron transport chain of both the algae was similar, PS II depicted greater sensitivity to the test metals used. The toxicity in both organisms was Cu > Ni > Fe. A. doliolum was, however, more sensitive to Cu and Ni, and C. vulgaris to Fe. Toxicity was generally dependent on metal uptake, which in turn was dependent on their concentrations in the external medium. A partial restoration of nutrient uptake, carbon fixation, and enzyme activities following supplementation of exogenous ATP suggests that ATP regulates toxicity through chelation.展开更多
为了研究铜绿微囊藻 Microcystis aeruginosa PCC7820中是否存在内源昼夜节律,检测了其细胞中的三磷酸腺苷(ATP)含量和细胞分裂的日变化规律。通过生物发光法检测的细胞内 ATP 含量变化结果表明,在12h光/12h暗(L/D)周期环境下该藻的 ATP...为了研究铜绿微囊藻 Microcystis aeruginosa PCC7820中是否存在内源昼夜节律,检测了其细胞中的三磷酸腺苷(ATP)含量和细胞分裂的日变化规律。通过生物发光法检测的细胞内 ATP 含量变化结果表明,在12h光/12h暗(L/D)周期环境下该藻的 ATP 含量呈现明显的近似24h 的昼夜周期性变化,且这种周期性变化在连续光照条件下能至少持续运行3个周期,周期长度具有温度补偿效应,而光照和温度的改变能重置昼夜节律的时相,这说明铜绿微囊藻 ATP 含量的昼夜节律变化受到内源生物钟的控制。通过对细胞数、细胞大小和细胞分裂素含量的检测发现,传代时间为38.4h 的铜绿微囊藻的细胞分裂也呈现受生物钟调控的昼夜节律性,这种昼夜节律变化可能是生物钟通过一种门控机制调控 M.aeruginosa PCC7820的细胞分裂时相而产生的。展开更多
基金supported by the Fund of China Agriculture Research System(CARS-37)the Genetically Modified Organisms Breeding Major Projects of China(2009ZX08011-031B)+1 种基金the Basic Research Fund of IAS,CAAS(2010jc-3-1)the National Natural Science Foundation of China(31001011)
文摘To our knowledge,no single study has systemically compared cryopreservation efficiencies of bovine blastocysts derived from in vitro fertilization （IVF）,intracytoplasmic sperm injection （ICSI） and somatic cell nuclear transfer （SCNT） by controlled freezing and vitrification.This experiment,therefore,was designed to compare the cryopreservation of these blastocysts with controlled freezing and OPS vitrification.Adenosine-5’-triphosphate （ATP） content and reactive oxygen species （ROS） level in blastocysts were also analyzed.Firstly,for each type of blastocyst （IVF,ICSI or SCNT）,significant differences were observed between the survival rates of the controlled freezing （（81.56±2.33）,（68.18±4.72） or （47.89±5.83）%） and OPS vitrification groups （（92.24±4.54）,（82.40±3.76） or （78.71±5.91）%;P〈0.05）.Secondly,for each type of blastocyst （IVF,ICSI or SCNT）,ATP content was significantly decreased after controlled freezing or vitrification,and the ATP content in the controlled freezing group （0.43±0.06）,（0.35±0.05） or （0.21±0.02） pmol） was significantly lower than that found in the OPS vitrification group （0.62±0.04）,（0.46±0.03） or （0.30±0.01） pmol;P〈0.05）.Thirdly,ROS level in fresh IVF （（47.33±3.56） c.p.s （counted photons per second）,ICSI （（36.51±2.58） c.p.s） or SCNT blastocysts （（26.44±1.49） c.p.s） was significantly lower than that found in the OPS vitrification group （（72.14±4.31）,（58.89±3.89） or （40.11±5.73） c.p.s;P〈0.05）,but higher than that of the controlled freezing group （34.41±3.32）,（23.13±1.26） or （15.46±2.45） c.p.s;P〈0.05）.The present study indicated that vitrification is more efficient in the cryopreservation of bovine blastocysts derived from IVF,ICSI or SCNT than controlled freezing.Furthermore,both vitrification and controlled freezing significantly altered the ATP content and ROS level in those blastocysts.
文摘To investigate the relationship between the ATP content in human oocytes and the deve-lopmental potential of human oocytes, unfertilized oocytes from clinical IVF and immature oocytes unsuitable for ICSI were collected. The ATP content of these unfertilized and in vitro matured eggs were determined quantitatively by measuring the luminescence using an ATP-dependent bioluminescence assay. The result showed that the ATP content of unfertilized oocytes was higher than in vitro matured ones (2.20±0.67 pmol vs 1.72±0.49 pmol, P<0.05 ). In unfertilized oocytes, the ATP content of those whose fertilization rates (FR) was higher than 50 % was 2.43±0.60 pmol, which was significantly different from those whose FR was less than 50 % (1.72±0.56 pmol), while in in vitro matured oocytes, the ATP content of those whose FR more than 50 % was 1.8±0.44 pmol, slightly higher than those less than 50 % (1.55±0.40 pmol), without statistical significance. There was a tendency that the ATP content of oocytes of pregnant patients was higher than those of controls, but the sample number was too small to show any significance in statistics. Briefly, there was positive correlation between the ATP content in oocytes and developmental potential of oocytes.
文摘This study demonstrates a concentration dependent inhibition of carbon fixation, O2 evolution, photosynthetic electron transport chain and ATP content of A. doliolum and C. vulgaris by Cu, Ni and Fe. Although the mode of inhibition of photosynthetic electron transport chain of both the algae was similar, PS II depicted greater sensitivity to the test metals used. The toxicity in both organisms was Cu > Ni > Fe. A. doliolum was, however, more sensitive to Cu and Ni, and C. vulgaris to Fe. Toxicity was generally dependent on metal uptake, which in turn was dependent on their concentrations in the external medium. A partial restoration of nutrient uptake, carbon fixation, and enzyme activities following supplementation of exogenous ATP suggests that ATP regulates toxicity through chelation.
文摘为了研究铜绿微囊藻 Microcystis aeruginosa PCC7820中是否存在内源昼夜节律,检测了其细胞中的三磷酸腺苷(ATP)含量和细胞分裂的日变化规律。通过生物发光法检测的细胞内 ATP 含量变化结果表明,在12h光/12h暗(L/D)周期环境下该藻的 ATP 含量呈现明显的近似24h 的昼夜周期性变化,且这种周期性变化在连续光照条件下能至少持续运行3个周期,周期长度具有温度补偿效应,而光照和温度的改变能重置昼夜节律的时相,这说明铜绿微囊藻 ATP 含量的昼夜节律变化受到内源生物钟的控制。通过对细胞数、细胞大小和细胞分裂素含量的检测发现,传代时间为38.4h 的铜绿微囊藻的细胞分裂也呈现受生物钟调控的昼夜节律性,这种昼夜节律变化可能是生物钟通过一种门控机制调控 M.aeruginosa PCC7820的细胞分裂时相而产生的。
