Cell-free expression system is a technology for the synthesis of proteins in vitro.The system is a platform for several bioengineering projects,e.g.cell-free metabolic engineering,evolutionary design of experiments,an...Cell-free expression system is a technology for the synthesis of proteins in vitro.The system is a platform for several bioengineering projects,e.g.cell-free metabolic engineering,evolutionary design of experiments,and synthetic minimal cell construction.Bacterial cell-free protein synthesis system(CFPS)is a robust tool for synthetic biology.The bacteria lysate,the DNA,and the energy module,which are the three optimized sub-systems for in vitro protein synthesis,compose the integrated system.Currently,an optimized E.coli cell-free expression system can produce up to^2.3 mg/mL of a fluorescent reporter protein.Herein,I will describe the features of ATP-regeneration systems for in vitro protein synthesis,and I will present a machine-learning experiment for optimizing the protein yield of E.coli cell-free protein synthesis systems.Moreover,I will introduce experiments on the synthesis of a minimal cell using liposomes as dynamic containers,and E.coli cell-free expression system as biochemical platform for metabolism and gene expression.CFPS can be further integrated with other technologies for novel applications in environmental,medical and material science.展开更多
文摘Cell-free expression system is a technology for the synthesis of proteins in vitro.The system is a platform for several bioengineering projects,e.g.cell-free metabolic engineering,evolutionary design of experiments,and synthetic minimal cell construction.Bacterial cell-free protein synthesis system(CFPS)is a robust tool for synthetic biology.The bacteria lysate,the DNA,and the energy module,which are the three optimized sub-systems for in vitro protein synthesis,compose the integrated system.Currently,an optimized E.coli cell-free expression system can produce up to^2.3 mg/mL of a fluorescent reporter protein.Herein,I will describe the features of ATP-regeneration systems for in vitro protein synthesis,and I will present a machine-learning experiment for optimizing the protein yield of E.coli cell-free protein synthesis systems.Moreover,I will introduce experiments on the synthesis of a minimal cell using liposomes as dynamic containers,and E.coli cell-free expression system as biochemical platform for metabolism and gene expression.CFPS can be further integrated with other technologies for novel applications in environmental,medical and material science.
