质膜H^(+)-ATPase基因对铝胁迫下水稻硝态氮吸收的 调控作用

为探讨细胞质膜ATP酶(PM H^(+)-ATPase)基因对铝胁迫下水稻(Oryza sativa L.)吸收硝态氮(NO_(3)^(-)-N)的调控,本试验以2个水稻品种峰1A和峰1A优5为研究对象,以无铝处理幼苗为对照,分析铝胁迫下水稻根尖PM H^(+)-ATPase活性、H^(+)-泵活性、H^(+)通量、PM H^(+)-ATPase基因家族(OsA1~OsA10)表达水平、PM H^(+)-ATPase和14-3-3蛋白的互作水平以及NO_(3)^(-)-N吸收量。结果表明,与对照相比,铝胁迫下2个水稻品种根尖PM H^(+)-ATPase活性、H^(+)-泵活性和根尖H^(+)通量下降,PM H^(+)-ATPase和14-3-3蛋白的互作水平下降。铝胁迫抑制了OsA1、OsA5、OsA7和OsA8基因的表达,其中OsA7的表达水平显著下调,铝胁迫下峰1A和峰1A优5的OsA7表达量仅为对照组的21%和39%。NO_(3)^(-)-N吸收量较对照下降,分别为对照的81%和85%。综上,PM H^(+)-ATPase基因的表达水平影响水稻对NO_(3)^(-)-N的吸收能力,OsA1、OsA5、OsA7和OsA8在铝胁迫下水稻吸收NO_(3)^(-)-N过程中起关键的调控作用。本研究结果可为增强酸铝条件下水稻吸收NO_(3)^(-)-N的能力,促进水稻生长及增产提供理论依据。

Nucleotide Contribution to the Functioning of SERT, Na /K ATPase and GPCR Proteins

Purine nucleotides are crucial for the effective operation of cell membrane proteins maintaining the neurotransmitter responses of 5-HT. Major protein targets in the treatment of depression include SERT, N/K ATPase and GPCR. Each protein target is responsive to a specific complement of drugs: antidepressants (SERT), lithium and cardiogenic steroids (N/K ATPase), 5-HT receptor ligands (GPCR). Computational software is useful for comparing molecular similarity within ligand-ligand and ligand-nucleotide structures. Previous studies demonstrate that GPCR ligands of different pharmacologic classes display relative molecular similarity to nucleotide structures. The current study applies this methodology to compound structures modulating SERT and N/K ATPase receptors. Minimum energy conformers of SERT antagonists demonstrate relative molecular similarity to the structural template of GTP nucleotide. GTP template fits of 5-HT and psilocin are similar, whereas a SERT-like fit is one of several for the ketamine structure. Endogenous and pharmaceutical modulators of Na/K ATPase relate to adenine nucleotide. The fits of cardiogenic steroids to a cGMP template demonstrate similarities and differences between compounds. Relative molecular similarity within the structures of hormones, drugs and nucleotides has implications for neurotransmitter transport and cell signal transduction processes.

盐度渐变对日本囊对虾非特异性免疫酶、ATPase酶和抗氧化酶活力的影响

为研究盐度渐变对日本囊对虾的机体免疫的影响,实验设置了4个盐度梯度分别为26、22、18和14,统计了各盐度下日本囊对虾累计死亡率,检测了日本囊对虾体内血清总蛋白(TP)含量、碱性磷酸酶(AKP)和酸性磷酸酶(ACP)、血清ATP酶(Na^(+)/K^(+)-ATPase和T-ATPase)、超氧化物歧化酶(SOD)和多酚氧化酶(PPO)的动态变化。结果显示,盐度渐变胁迫下各盐度组累计死亡率的大小为14>18>22>26,血清蛋白含量随胁迫强度的增加和胁迫时间的延长呈下降趋势,后随着盐度回升而上升。24~72 h ACP和AKP活力表现为盐度组14<18<22<26,且随时间的延长呈下降趋势,Na^(+)/K^(+)-ATPase和T-ATPase活力显示出盐度梯度差异显著,14盐度组显著高于其它盐度组(P<0.05),SOD活力与PPO活力受盐度胁迫强度和胁迫时间的影响显著,盐度胁迫强度越强,SOD活力与对照组的差异越显著(P<0.05),血清PPO活力的变化趋势越明显。日本囊对虾机体免疫受盐度胁迫显著,研究结果为日本囊对虾的健康养殖提供重要的理论指导及借鉴。

百合ATP合成酶家族基因ATPase3的克隆和生物信息学分析

【目的】为有效解决百合花粉污染问题,利用分子生物学技术培育雄性不育的无花粉百合,从基因克隆和生物信息学角度研究百合ATP合成酶家族基因ATPase3在导致百合雄性不育过程中的作用,为应用该基因进行百合无花粉分子育种提供一定的理论基础。【方法】以百合可育系与不育系花粉母细胞时期花药为试验材料,在百合可育系与不育系转录组测序结果中筛选出1个花粉母细胞时期显著上调表达的ATPase3基因,利用荧光定量qRT-PCR技术检测ATPase3基因在百合可育系与不育系花粉母细胞时期不同部位的相对表达量。结合qRT-PCR与Race技术扩增得到百合花药ATPase3基因cDNA全长序列,并通过Prot Param、Prot Scale、NCBI Conserved Domain、SWISS-MODEL等在线生物信息学数据库对百合ATPase3同源性、蛋白结构、理化性质等进行生物信息学分析。【结果】百合ATPase3基因在花器官中表达量最高,其中在可育系花药中的表达量是不育系的7倍。通过克隆得到百合ATPase3基因,该基因全长327 bp,编码109个氨基酸,含有1个典型的保守ATPase3结构域;编码蛋白相对分子量约为12.14 KD,理论等电点为7.71,为疏水性蛋白,存在跨膜结构,并预测蛋白二级和三级结构。生物信息学分析说明该预测蛋白具有典型ATP合成酶特征。同源序列分析表明,该蛋白与拟南芥质膜ATPase3同源性最为接近,与拟南芥H[+]ATPase1,H[+]ATPase2,H[+]ATPase3,H[+]ATPase8同源性也较为接近。推测该蛋白属于百合中提供能量的质膜离子转运蛋白,它在百合不育系花药中表达量下调,提供能量不足,导致雄性不育。【结论】本研究验证了ATPase3基因与百合雄性不育相关,并成功克隆得到了ATPase3基因,对该基因进行了生物信息学分析和功能预测,为百合ATPase3基因编码的质膜离子转运蛋白功能缺失导致百合雄性不育现象的发生提供一定的理论基础。

利拉鲁肽通过调控自噬和Na^(+),K^(+)-ATPase活性抑制高糖诱导的心肌细胞肥大

目的探讨利拉鲁肽(liraglutide,LRG)抑制高糖(HG)诱导的心肌细胞肥大的可能机制。方法体外培养H9c2细胞,分为对照(CON)组、HG组、低、中、高剂量LRG(LRG-L、LRG-M、LRG-H)组、LRG-H+自噬抑制剂3-甲基腺嘌呤(3-MA)组。鬼笔环肽染色观察细胞表面积;试剂盒测定细胞膜Na^(+),K^(+)-ATPase(NKA)活性;Real time-PCR和Western blot测定NKAα1、NKAα2 mRNA和蛋白表达;单丹磺胺戊二胺(MDC)荧光染色观察自噬囊泡数量;Western blot测定肥大标志基因(ANP、β-MHC)、自噬标志基因(Beclin-1、LC3、p62)蛋白表达。随后将H9c2细胞分为CON组、HG组、LRG-H组、LRG-H+Sirt1抑制剂EX 527组、LRG-H+AMPK抑制剂Compound C(CC)组,再次检测上述指标;Western blot测定Sirt1/AMPK/mTOR通路相关蛋白表达。结果LRG可抑制心肌细胞肥大,下调ANP、β-MHC蛋白表达;LRG可促进NKA活性恢复,上调NKAα2 mRNA和蛋白表达;LRG可增加自噬囊泡数量,上调Beclin-1、LC3-Ⅱ/LC3-Ⅰ蛋白表达,下调p62蛋白表达;LRG可上调Sirt1、p-AMPK/AMPK蛋白表达,下调p-mTOR/mTOR蛋白表达;使用自噬抑制剂3-MA、Sirt1抑制剂EX 527、AMPK抑制剂CC则部分逆转了LRG的作用。结论LRG可抑制高糖所致心肌细胞肥大,其机制与调控Sirt1/AMPK/mTOR通路激活自噬及促进NKA活性恢复有关。

V-ATPase H表达量下调增强棉铃虫对Cry1Ac的抗性

棉铃虫Helicoverpa armigera是世界性重要农业害虫。目前防治棉铃虫的主要手段是种植转苏云金芽胞杆菌Bacillus thuringiensis(Bt)杀虫蛋白的转基因作物。本文旨在研究棉铃虫V-ATPase H在Cry1Ac蛋白毒力和抗性中的作用。利用实时荧光定量qRT-PCR技术分析V-ATPase H在Cry1Ac抗、感品系棉铃虫幼虫中肠及敏感品系棉铃虫幼虫受Cry1Ac诱导后的表达情况;在昆虫Sf9细胞中过表达V-ATPase H对其进行细胞定位,通过细胞毒力试验验证其对Cry1Ac毒力的影响。结果发现棉铃虫V-ATPase H基因在抗性品系中低表达,并且V-ATPase H在受到Cry1Ac诱导时也低表达;在Sf9细胞内表达V-ATPase H蛋白发现其在整个细胞中都有分布,过表达该蛋白后增强了细胞对Cry1Ac蛋白的敏感性。结果表明V-ATPase H参与Cry1Ac蛋白的毒力。

Cloning of vacuolar H^+ -ATPase subunit c genes from Japanese iris, and functional characterization in yeast

Five different isoforms （IrlVHA-c1-c5） of V-ATPase subunit c （VHA-c） were cloned from a Japanese iris （Iris lactea Pall. var. chinensis Fisch. Koidz） cDNA library using degenerate primers PCR and the 5＇-RACE technique. The sequence analysis showed the open reading frame （ORF） of the IrlVHA-c1 c5 to be 495 bp, corresponding to a protein of 164 amino acids. Among the five isoforms, IrlVHA-c1 and IrlVHA-c2 are completely homologous. The IrlVHA-c protein is localized at the vacuolar membrane as indicated by a green fluorescent protein （GFP） marker. Its over-expression in yeast could enhance yeast tolerance to NaCl stress. These results show that there are at least five genes encoding different isoforms of IrlVHA-c in Japanese iris and IrlVHA-c is important for the function of V-ATPase.

Cloning and expression analysis of GhDET3, a vacuolar H^+-ATPase subunit C gene, from cotton

Vacuolar H^+-ATPase was regarded as a key enzyme promoting the fiber cell elongation in cotton (Gossypium hirsuturm L.) through regulating turgor-driven pressure involved in polarity expansion of single cell fiber. The DET3, a V-ATPase subunit C, plays an important role in assembling subunits and regulating the enzyme activity, and is involved in Brassinosteroid-induced cell elongation. To analyze the function of GhDET3 on the elongation of cotton fibers, seven candidates of ESTs were screened and contigged for a 5'-upstream sequence, and the 3'-RACE technique was used to clone the 3'-downstream sequence for the full length of GhDET3 gene. The full length of the target clone was 1,340 bp, including a 10 bp 5'-UTR, an ORF of 1,134 bp, and a 196 bp 3'-UTR. This cDNA sequence encoded a polypepide of 377 amino acid residues with a predicted molecular mass of 43 kDa and a basic isoelectric point of 5.58. Furthermore, a length of 3,410 bp sequence from genomic DNA of GhDET3 was also cloned by PCR. The deduced amino acid sequence had a high homology with DET3 from Arabidopsis, rice, and maize. Quantitative real-time PCR (qRT-PCR) analysis showed that the GhDET3 expression pattern was ubiquitous in all the tissues and organs detected. The result also revealed that the accumulation of GhDET3 mRNA reached the highest profile at the fiber elongation stage in 12 DPA (days post anthesis) fibers, compared with the lowest level at the fiber initiation stage in 0 DPA ovules (with fibers). The transcript accumulation in fibers and ovules shared the similar variation tendency. In addition, in vitro ovule culture experiment demonstrated that exogenous 24-EBL treatment to 4 DPA ovules (with fibers) was capable of increasing the expression level of GhDET3, and the mRNA accumulation of GhDET3 increased in transgenic FBP7::GhDET2 cotton fibers in vivo. These results indicate that GhDET3 gene plays a crucial role in cotton fiber elongation.

Na^+/K^+-ATPase α-subunit in swimming crab Portunus trituberculatus: molecular cloning, characterization, and expression under low salinity stress

A bstract Na + /K +-ATPases are membrane-associated enzymes responsible for the active transport of Na + and K + ions across cell membranes, generating chemical and electrical gradients. These enzymes' α-subunit provides catalytic function, binding and hydrolyzing ATP, and itself becoming phosphorylated during the transport cycle. In this study, Na+ /K +-ATPase α-subunit c DNA was cloned from gill tissue of the swimming crab P ortunus trituberculatus by reverse-transcription polymerase chain reaction(RT-PCR) and rapid amplification of c DNA end methods. Analysis of the nucleotide sequence revealed that the c DNA had a full-length of 3 833 base pairs(bp), with an open reading frame of 3 120 bp, 5' untranslated region(UTR) of 317 bp, and 3' UTR of 396 bp. The sequence encoded a 1 039 amino acid protein with a predicted molecular weight of 115.57 k Da and with estimated p I of 5.21. It was predicted here to possess all expected features of Na+ /K +-ATPase members, including eight transmembrane domains, putative ATP-binding site, and phosphorylation site. Comparison of amino acid sequences showed that the P. trituberculatus α-subunit possessed an overall identity of 75%–99% to that of other organisms. Phylogenetic analysis revealed that this α-subunit was in the same category as those of crustaceans. Quantitative real-time RT-PCR analysis indicated that this α-subunit's transcript were most highly expressed in gill and lowest in muscle. RT-PCR analysis also revealed that α-subunit expression in crab gill decreased after 2 and 6 h, but increased after 12, 24, 48, and 72 h. In addition, α-subunit expression in hepatopancreas of crab decreased after 2–72 h. These facts indicated that the crab's Na+ /K +-ATPase α-subunit was potentially involved in the observed acute response to low salinity stress.

Analysis of the potential biological value of pyruvate dehydrogenase E1 subunitβin human cancer

BACKGROUND The pyruvate dehydrogenase E1 subunitβ(PDHB)gene which regulates energy metabolism is located in mitochondria.However,few studies have elucidated the role and mechanism of PDHB in different cancers.AIM To comprehensive pan-cancer analysis of PDHB was performed based on bioinformatics approaches to explore its tumor diagnostic and prognostic value and tumor immune relevance in cancer.In vitro experiments were performed to examine the biological regulation of PDHB in liver cancer.METHODS Pan-cancer data related to PDHB were obtained from the Cancer Genome Atlas(TCGA)database.Analysis of the gene expression profiles of PDHB was based on TCGA and Genotype Tissue Expression Dataset databases.Cox regression analysis and Kaplan-Meier methods were used to assess the correlation between PDHB expression and survival prognosis in cancer patients.The correlation between PDHB and receiver operating characteristic diagnostic curve,clinicopathological staging,somatic mutation,tumor mutation burden(TMB),microsatellite instability(MSI),DNA methylation,and drug susceptibility in pan-cancer was also analyzed.Various algorithms were used to analyze the correlation between PDHB and immune cell infiltration and tumor chemotaxis environment,as well as the co-expression analysis of PDHB and immune checkpoint(ICP)genes.The expression and functional phenotype of PDHB in single tumor cells were studied by single-cell sequencing,and the functional enrichment analysis of PDHB-related genes was performed.The study also validated the level of mRNA or protein expression of PDHB in several cancers.Finally,in vitro experiments verified the regulatory effect of PDHB on the proliferation,migration,and invasion of liver cancer.RESULTS PDHB was significantly and differently expressed in most cancers.PDHB was significantly associated with prognosis in patients with a wide range of cancers,including kidney renal clear cell carcinoma,kidney renal papillary cell carcinoma,breast invasive carcinoma,and brain lower grade glioma.In some cancers,PDHB expression was clearly associated with gene mutations,clinicopathological stages,and expression of TMB,MSI,and ICP genes.The expression of PDHB was closely related to the infiltration of multiple immune cells in the immune microenvironment and the regulation of tumor chemotaxis environment.In addition,single-cell sequencing results showed that PDHB correlated with different biological phenotypes of multiple cancer single cells.This study further demonstrated that down-regulation of PDHB expression inhibited the proliferation,migration,and invasion functions of hepatoma cells.CONCLUSION As a member of pan-cancer,PDHB may be a novel cancer marker with potential value in diagnosing cancer,predicting prognosis,and in targeted therapy.

Genetic diversity of the S-type small subunit ribosomal RNA gene of Plasmodium knowlesi isolates from Sabah,Malaysian Borneo and Peninsular Malaysia

Objective:To determine the genetic diversity of Plasmodium(P.)knowlesi isolates from Sabah,Malaysian Borneo and Peninsular Malaysia,targeting the S-type SSU rRNA gene and including aspects of natural selection and haplotype.Methods:Thirty-nine blood samples infected with P.knowlesi were collected in Sabah,Malaysian Borneo and Peninsular Malaysia.The S-type SSU rRNA gene was amplified using polymerase chain reaction,cloned into a vector,and sequenced.The natural selection and haplotype of the S-type SSU rRNA gene sequences were determined using DnaSP v6 and illustrated using NETWORK v10.This study's 39 S-type SSU rRNA sequences and eight sequences from the Genbank database were subjected to phylogenetic analysis using MEGA 11.Results:Overall,the phylogenetic analysis showed no evidence of a geographical cluster of P.knowlesi isolates from different areas in Malaysia based on the S-type SSU rRNA gene sequences.The S-type SSU rRNA gene sequences were relatively conserved and with a purifying effect.Haplotype sharing of the S-type SSU rRNA gene was observed between the P.knowlesi isolates in Sabah,Malaysian Borneo,but not between Sabah,Malaysian Borneo and Peninsular Malaysia.Conclusions:This study suggests that the S-type SSU rRNA gene of P.knowlesi isolates in Sabah,Malaysian Borneo,and Peninsular Malaysia has fewer polymorphic sites,representing the conservation of the gene.These features make the S-type SSU rRNA gene suitable for comparative studies,such as determining the evolutionary relationships and common ancestry among P.knowlesi species.

Cognitive dysfunction in schizophrenia patients caused by downregulation of γ-aminobutyric acid receptor subunits

BACKGROUND The expression pattern of gamma aminobutyric acid(GABA)receptor subunits are commonly altered in patients with schizophrenia,which may lead to nerve excitation/inhibition problems,affecting cognition,emotion,and behavior.AIM To explore GABA receptor expression and its relationship with schizophrenia and to provide insights into more effective treatments.METHODS This case-control study enrolled 126 patients with schizophrenia treated at our hospital and 126 healthy volunteers who underwent physical examinations at our hospital during the same period.The expression levels of the GABA receptor subunits were detected using 1H-magnetic resonance spectroscopy.The recognized cognitive battery tool,the MATRICS Consensus Cognitive Battery,was used to evaluate the scores for various dimensions of cognitive function.The correlation between GABA receptor subunit downregulation and schizophrenia was also analyzed.RESULTS Significant differences in GABA receptor subunit levels were found between the case and control groups(P<0.05).A significant difference was also found between the case and control groups in terms of cognitive function measures,including attention/alertness and learning ability(P<0.05).Specifically,as the expression levels of GABRA1(α1 subunit gene),GABRB2(β2 subunit gene),GABRD(δsubunit),and GABRE(εsubunit)decreased,the severity of the patients’condition increased gradually,indicating a positive correlation between the downregulation of these 4 receptor subunits and schizophrenia(P<0.05).However,the expression levels of GABRA5(α5 subunit gene)and GABRA6(α6 subunit gene)showed no significant correlation with schizophrenia(P>0.05).CONCLUSION Downregulation of the GABA receptor subunits is positively correlated with schizophrenia.In other words,when GABA receptor subunits are downregulated in patients,cognitive impairment becomes more severe.

Cardioprotective Potential of Cymbopogon citratus Essential Oil against Isoproterenol-induced Cardiomyocyte Hypertrophy:Possible Involvement of NLRP3 Inflammasome and Oxidative Phosphorylation Complex Subunits

Objective:Cymbopogon citratus(DC.)Stapf is a medicinal and edible herb that is widely used for the treatment of gastric,nervous and hypertensive disorders.In this study,we investigated the cardioprotective effects and mechanisms of the essential oil,the main active ingredient of Cymbopogon citratus,on isoproterenol(ISO)-induced cardiomyocyte hypertrophy.Methods:The compositions of Cymbopogon citratus essential oil(CCEO)were determined by gas chromatography-mass spectrometry.Cardiomyocytes were pretreated with 16.9µg/L CCEO for 1 h followed by 10µmol/L ISO for 24 h.Cardiac hypertrophy-related indicators and NLRP3 inflammasome expression were evaluated.Subsequently,transcriptome sequencing(RNA-seq)and target verification were used to further explore the underlying mechanism.Results:Our results showed that the CCEO mainly included citronellal(45.66%),geraniol(23.32%),and citronellol(10.37%).CCEO inhibited ISO-induced increases in cell surface area and protein content,as well as the upregulation of fetal gene expression.Moreover,CCEO inhibited ISO-induced NLRP3 inflammasome expression,as evidenced by decreased lactate dehydrogenase content and downregulated mRNA levels of NLRP3,ASC,CASP1,GSDMD,and IL-1β,as well as reduced protein levels of NLRP3,ASC,pro-caspase-1,caspase-1(p20),GSDMD-FL,GSDMD-N,and pro-IL-1β.The RNA-seq results showed that CCEO inhibited the increase in the mRNA levels of 26 oxidative phosphorylation complex subunits in ISO-treated cardiomyocytes.Our further experiments confirmed that CCEO suppressed ISO-induced upregulation of mt-Nd1,Sdhd,mt-Cytb,Uqcrq,and mt-Atp6 but had no obvious effects on mt-Col expression.Conclusion:CCEO inhibits ISO-induced cardiomyocyte hypertrophy through the suppression of NLRP3 inflammasome expression and the regulation of several oxidative phosphorylation complex subunits.

Potassium Depletion Stimulates Beta-Subunit of Colonic H⁺-K⁺-ATPase in Mice

H+-K+-ATPase (HKA) is composed of two different subunits: an alpha and a beta subunit. Previous studies have shown that in the kidney gastric HKA (HKA alpha 1) predominates under normal dietary conditions while colonic HKA (HKA alpha 2) predominates under potassium depleted conditions [1]. The purpose of the current study was to elucidate the association between the beta and different alpha subunits from stomach, colon and kidney under normal and potassium depleted conditions. Black Swiss mice were fed a potassium-free diet for 2 weeks, beta subunit expression of HKA in stomach mucosae, colon mucosae and renal outer medulla was examined and compared between normal diet and potassium depleted diet. In wild type (WT) mice, the concentrations of the beta subunit under potassium deficient conditions were found significantly increased compared with normal dietary conditions in colon mucosae (8.27 ± 0.73 vs 6.62 ± 0.55 μg/μl, n = 7, p = 0.0416), whereas in cHKA (HKA alpha 2) mice colon mucosae, the concentrations of the beta subunit were statistically the same (5.05 ± 0.31 vs 4.76 ± 0.37 μg/μl, n = 13, p = 0.2833), and the concentration of the beta subunit stayed the same in renal outer medulla and stomach mucosae as well. The data indicate that potassium deficiency results in a significant increase in the levels of HKA beta subunit concentration in the colonic tissue of WT mice. The results indicate that the HKA beta subunit associates with the cHKA (HKA alpha 2) in order to mediate bicarbonate reabsorption under potassium depletion (hypokalemia)

Construction of Recombinant Plasmid Containing S.Mutans F-ATPase β Subunit Gene

objective: construct a homologous recombinant plasmid which was expected to be transformed into S.mutans Methods: a region at the 5’terminus of the S.mutans F-ATPase β subunit gene was amplified by PCR, the PCR product was inserted into vector pVA891,yielding recombinant plasmid.Results: the DNA sequence of the recombinant plasmid was identified correct in whole by restriction endonuclease and DNA sequence techniques.Conclusion: the recombinant plasmid of S.mutans DNA was cloned in effect,it may assist in construction of homologues recombinant mutant.

水杨酸对镉胁迫下葡萄根系质膜ATPase和自由基的影响

【目的】研究水杨酸对镉胁迫下葡萄根系特性的影响,探讨缓解葡萄镉伤害的途径。【方法】以‘泽香’葡萄扦插苗为试材,在水培条件下,研究水杨酸预处理对氯化镉胁迫下葡萄根系活力、质膜H+-ATPase和Ca2+-ATPase活性以及活性氧和一氧化氮(NO)生成的影响。【结果】0.10mmol·L-1氯化镉能够提高根系活力和质膜Ca2+-ATPase活性;1.0mmol·L-1氯化镉明显促进根系超氧阴离子()、H2O2和NO的生成,显著抑制根系活力及质膜H+-ATPase和Ca2+-ATPase活性。50μmol·L-1水杨酸显著降低1.0mmol·L-1氯化镉处理下根系、H2O2和NO的生成,阻止根系活力及质膜H+-ATPase和Ca2+-ATPase活性下降,而随着浓度升高至200μmol·L-1,水杨酸的这种作用减弱。【结论】水杨酸缓解葡萄根系镉伤害的适宜浓度为50μmol·L-1;在该浓度下,水杨酸通过降低镉胁迫下自由基的产生而减轻镉对葡萄根系活力和质膜ATPase的损伤。

我国玉米品种更替过程中根系生理特性的演进 Ⅰ.根系活性与ATPase活性的变化

我国 195 0 s～ 1990 s期间主要推广玉米品种 (Zea mays L .)根系活力随播种后天数的变化可用高斯模型作较好地模拟 ,根系活力随生育进程的推进先增后降 ,呈单峰曲线变化 ,在籽粒灌浆期 (播种后 6 7～ 74天 )达到高峰 ;上层 (0～ 2 0 cm )根系活力高于下层。随玉米品种更替根系活力增强 ,在 4 0～ 10 0 cm根层和生育后期更为突出。对根系生长的高斯方程积分 ,求得“根系活力日积”(RVDI) ,当代品种各生育阶段的 RVDI远高于 1970 s和 195 0 s品种 ,且在灌浆期间 (播种后 6 7天至 90天 )这种差别最大。根系中可溶性蛋白含量、 ATPase活性随品种更替呈递增趋势 ,且在距离植株 10～ 15 cm的横向空间内活性提高最大 ,当代品种在 10～ 2 5 cm的横向空间内

高温胁迫对花生幼苗光合速率、叶绿素含量、叶绿体Ca^(2+)-ATPase、Mg^(2+)-ATPase及Ca^(2+)分布的影响

将水培后盆栽的花生幼苗,置于培养箱42℃高温培养,定时测定幼苗叶光合速率、叶绿素含量和叶绿体Ca2+-ATPase、Mg2+-ATPase的相对活性,并观察幼叶细胞内Ca2+分布的变化。试验结果表明:高温胁迫过程中,光合速率及叶绿素含量都随处理时间的延伸而下降,并呈显著正相关;叶绿体Ca2+-ATPase和Mg2+-ATPase高温胁迫过程中相对活性呈先升后降趋势,Ca2+-ATPase热敏性高于Mg2+-ATPase;高温胁迫过程中,Ca2+具有从胞外转运到胞质内和叶绿体中的趋势,Ca2+能够稳定高温胁迫下叶肉细胞膜和叶绿体的超微结构。

失血性休克肠上皮细胞线粒体DNA ATPase6,8亚基基因的改变

目的 观察失血性休克大鼠肠上皮细胞mtDNAATPase 6,8亚基基因的改变。方法 将 6只Wistar大鼠分成单纯手术组、休克组。分别提取mtDNA ,采用PCR技术扩增后测序 ,检测突变点。结果 Wistar大鼠mtDNAATPase 6,8亚基基因存在多态性 ,休克组存在A 7797缺失和一定数量点突变 :A7863G ,G795 0T ,C82 94G ,T85 0 5G。结论 失血性休克 5h ,肠上皮细胞mtDNAATPase 6,8亚基基因会发生突变 ,由此可导致所编码的氨基酸的改变 。

NO_3^-胁迫及恢复对黄瓜幼苗叶片叶绿素荧光参数及ATPase活性的影响

通过水培试验,探讨了不同NO3-浓度胁迫及恢复对黄瓜幼苗叶片叶绿素含量、叶绿素荧光参数及ATPase活性的影响.结果表明,胁迫7 d后,高浓度NO3-(168 mmol.L-1)可极显著提高叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量,极显著提高初始荧光(Fo)、Mg-ATPase和Ca-ATPase活性,而PSⅡ原初光能转化效率(Fv/Fm)、PSⅡ潜在活性(Fv/Fo)和PSⅡ光合电子传递量子效率(ΦPSⅡ),却随NO3-浓度的增加而降低.恢复7 d后,所有处理叶绿素和类胡萝卜素含量均低于对照;初始荧光基本都恢复至对照水平;PSⅡ原初光能转化效率和PSⅡ光合电子传递量子效率在NO3-浓度低于126 mmol.L-1时,基本恢复至对照水平,而高于这一水平时,仍显著低于对照;PSⅡ潜在活性在NO3-浓度为42和126 mmol.L-1的处理基本达对照水平,其它处理仍极显著低于对照;Mg-ATPase和Ca-ATPase活性均出现先降低后升高的变化趋势.