[目的]观察葱白提取物对非酒精性脂肪肝(NAFLD)模型大鼠ATP合酶Fo亚基6(ATPase6)、ATP合酶Fo亚基8(ATPase8)表达的影响,并探讨其作用机制。[方法]将50只雄性SD大鼠随机分为正常组、模型组、葱白低剂量组、葱白中剂量组、葱白高剂量组,每...[目的]观察葱白提取物对非酒精性脂肪肝(NAFLD)模型大鼠ATP合酶Fo亚基6(ATPase6)、ATP合酶Fo亚基8(ATPase8)表达的影响,并探讨其作用机制。[方法]将50只雄性SD大鼠随机分为正常组、模型组、葱白低剂量组、葱白中剂量组、葱白高剂量组,每组10只。采用高脂饮食喂养复制NAFLD模型大鼠,造模10周后,给予葱白提取物连续干预4周后,测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和甘油三酯(TG)、胆固醇(TC)的含量;苏木精-伊红(HE)染色法观察肝脏组织结构变化;Real time PCR检测大鼠肝脏组织中ATPase6、ATPase8 mRNA的表达。[结果]与正常组比较,模型组出现明显肝脂肪变性,血清ALT、AST、TC、TG水平明显升高(P<0.05),ATPase6、ATPase8 mRNA的表达明显降低(P<0.05);相较于模型组,葱白低、中、高剂量组肝脂肪变性均不同程度减轻;血清ALT、AST、TC、TG水平均不同程度降低(P<0.05),ATPase6、ATPase8 m RNA表达不同程度升高(P<0.05)。与葱白低剂量组比较,葱白中、高剂量组肝脂肪变性减轻明显,ALT、AST、TC、TG水平明显降低(P<0.05),ATPase6、ATPase8 mRNA水平明显升高(P<0.05),中、高剂量葱白组之间差异无统计学意义。[结论]葱白提取物能够改善肝脂肪变性,可能与提高ATPase6、ATPase8的表达,增加肝线粒体ATP合成有关。展开更多
To investigate the effects of hyperoxia on mitochondrial multienzyme complex Ⅲ(cytochrome,Cytb) and Ⅴ(ATPase6,8) in premature newborn rat lung,the 1-day-old preterm SD rats were randomly assigned to hyperoxia group ...To investigate the effects of hyperoxia on mitochondrial multienzyme complex Ⅲ(cytochrome,Cytb) and Ⅴ(ATPase6,8) in premature newborn rat lung,the 1-day-old preterm SD rats were randomly assigned to hyperoxia group and air group.The rats in hyperoxia group were con-tinuously exposed to 85% oxygen and those in air group to room air.After 1,4,7,10,14 day(s) of exposure,these rats were killed,total lung RNA was extracted and Cytb,ATPase6,8 mRNA were detected by reverse transcription polymerase chain reaction(RT-PCR).Western blotting was used to detect the expression of Cytb protein in lung tissue.The results showed that compared with air group,Cytb mRNA expression was significantly increased(P>0.05) after 1,4 day(s) of exposure.The gen-eral tendency decreased after 7 days,and its expression became weak but difference in mRNA ex-pression between the two groups was not significant(P>0.05).ATPase6 mRNA expression was sig-nificantly increased 1 day after the exposure(P<0.05) and did not show any significant change 4,7,10 days after the exposure(P>0.05).At the 14th day,ATPase6 mRNA expression was significantly increased(P<0.05).ATPase8 mRNA expression did not show any significant change 1,4,10 day(s) after the exposure(P>0.05).At the 7th and 14th day,ATPase8 mRNA expression was significantly increased(P<0.05).Western blotting showed that Cytb protein expression was increased 1,4 day(s) after the exposure,but the difference between the two groups was not significant(P>0.05).The gen-eral tendency was decreased after 7 days,and its expression became weak but difference was not sig-nificant 7,10 days after the exposure(P>0.05).At day 14 its expression became significantly weak(P<0.05).We are led to conclude that exposure to high concentrations of oxygen can significantly change the expression of Cytb and ATPase6,8,which results in uncoupling of oxidative phosphoryla-tion in mitochondrial respiration chain,and plays an important role in the mechanism of hyper-oxia-induced lung injury.展开更多
文摘[目的]观察葱白提取物对非酒精性脂肪肝(NAFLD)模型大鼠ATP合酶Fo亚基6(ATPase6)、ATP合酶Fo亚基8(ATPase8)表达的影响,并探讨其作用机制。[方法]将50只雄性SD大鼠随机分为正常组、模型组、葱白低剂量组、葱白中剂量组、葱白高剂量组,每组10只。采用高脂饮食喂养复制NAFLD模型大鼠,造模10周后,给予葱白提取物连续干预4周后,测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和甘油三酯(TG)、胆固醇(TC)的含量;苏木精-伊红(HE)染色法观察肝脏组织结构变化;Real time PCR检测大鼠肝脏组织中ATPase6、ATPase8 mRNA的表达。[结果]与正常组比较,模型组出现明显肝脂肪变性,血清ALT、AST、TC、TG水平明显升高(P<0.05),ATPase6、ATPase8 mRNA的表达明显降低(P<0.05);相较于模型组,葱白低、中、高剂量组肝脂肪变性均不同程度减轻;血清ALT、AST、TC、TG水平均不同程度降低(P<0.05),ATPase6、ATPase8 m RNA表达不同程度升高(P<0.05)。与葱白低剂量组比较,葱白中、高剂量组肝脂肪变性减轻明显,ALT、AST、TC、TG水平明显降低(P<0.05),ATPase6、ATPase8 mRNA水平明显升高(P<0.05),中、高剂量葱白组之间差异无统计学意义。[结论]葱白提取物能够改善肝脂肪变性,可能与提高ATPase6、ATPase8的表达,增加肝线粒体ATP合成有关。
文摘To investigate the effects of hyperoxia on mitochondrial multienzyme complex Ⅲ(cytochrome,Cytb) and Ⅴ(ATPase6,8) in premature newborn rat lung,the 1-day-old preterm SD rats were randomly assigned to hyperoxia group and air group.The rats in hyperoxia group were con-tinuously exposed to 85% oxygen and those in air group to room air.After 1,4,7,10,14 day(s) of exposure,these rats were killed,total lung RNA was extracted and Cytb,ATPase6,8 mRNA were detected by reverse transcription polymerase chain reaction(RT-PCR).Western blotting was used to detect the expression of Cytb protein in lung tissue.The results showed that compared with air group,Cytb mRNA expression was significantly increased(P>0.05) after 1,4 day(s) of exposure.The gen-eral tendency decreased after 7 days,and its expression became weak but difference in mRNA ex-pression between the two groups was not significant(P>0.05).ATPase6 mRNA expression was sig-nificantly increased 1 day after the exposure(P<0.05) and did not show any significant change 4,7,10 days after the exposure(P>0.05).At the 14th day,ATPase6 mRNA expression was significantly increased(P<0.05).ATPase8 mRNA expression did not show any significant change 1,4,10 day(s) after the exposure(P>0.05).At the 7th and 14th day,ATPase8 mRNA expression was significantly increased(P<0.05).Western blotting showed that Cytb protein expression was increased 1,4 day(s) after the exposure,but the difference between the two groups was not significant(P>0.05).The gen-eral tendency was decreased after 7 days,and its expression became weak but difference was not sig-nificant 7,10 days after the exposure(P>0.05).At day 14 its expression became significantly weak(P<0.05).We are led to conclude that exposure to high concentrations of oxygen can significantly change the expression of Cytb and ATPase6,8,which results in uncoupling of oxidative phosphoryla-tion in mitochondrial respiration chain,and plays an important role in the mechanism of hyper-oxia-induced lung injury.
