以细菌和古菌基因组5′UTR序列作为研究对象,分析在5′UTR的3个不同阅读框架中三联体AUG的分布,发现无论是细菌还是古菌基因组都在阅读框1中有非常明显的AUG缺失(depletion)。AUG的缺失表明在起始密码子上游的AUG很可能会对基因的翻译...以细菌和古菌基因组5′UTR序列作为研究对象,分析在5′UTR的3个不同阅读框架中三联体AUG的分布,发现无论是细菌还是古菌基因组都在阅读框1中有非常明显的AUG缺失(depletion)。AUG的缺失表明在起始密码子上游的AUG很可能会对基因的翻译起始产生影响。分析得知:绝大部分的AUG都是以uORF(upstream open reading frame)的形式出现的,uAUG(upstream AUG)的数量很少,特别是在阅读框1中,而且在细菌基因组的阅读框1中uAUG较多地出现在了含有SD序列的基因上游。比较发现,uAUG引导的序列在同义密码子使用上的偏好性较真正的编码序列差,这可能表明细菌和古菌在同义密码子使用上的偏好性也是决定基因准确地翻译起始的重要因素之一。展开更多
The 15th Power Systems Computation Conference (PSCC'05) will take place in Liege, Belgium,August 22-26, 2005. PSCC has developed into one of the premier conferences in the area. The emphasis is on both theoretical...The 15th Power Systems Computation Conference (PSCC'05) will take place in Liege, Belgium,August 22-26, 2005. PSCC has developed into one of the premier conferences in the area. The emphasis is on both theoretical developments as well as practical applications in the utility and manufacturing industries.展开更多
This paper mainly discusses the variation of cosmic ray intensitycaused by solar flares in Mar. and Aug. 1989. Qualitative comparisonand analysis have been made for the events in Mar. and Aug. especiallyfrom the diffe...This paper mainly discusses the variation of cosmic ray intensitycaused by solar flares in Mar. and Aug. 1989. Qualitative comparisonand analysis have been made for the events in Mar. and Aug. especiallyfrom the difference of the variation of cosmic ray intensity in BeijingTokoy, and Guangzhou, and the anisotropic character of cosmic rayintensity from East to West direction recorded by the scintillator telescope in Guangzhou. It is found that though the events of decreaseof cosmic ray in Mar. and Aug. both coused by solar flares, there aredifference between two events, either in the shape of the decrease of cosmic ray or in the energy distribution of the falling particles.1989 is one of the years of the nationwide joint observation for the 22’ Max. The scintillator multi-directional telescope in Guang-zhou Observatory took part in the work.展开更多
The relationship between the codon usage bias, gene expression level and the AUG context(from -20 to +6 positions relative to the initiator AUG codon) was examined in 541unigene sequences of rice. A significant correl...The relationship between the codon usage bias, gene expression level and the AUG context(from -20 to +6 positions relative to the initiator AUG codon) was examined in 541unigene sequences of rice. A significant correlation for CAI values (codon adaptationindex) was observed at five nucleotide positions (-19, -18, -9, -4, +5), eight (-19, -18,-14, -9, -6, -4, -1, +5) for CPP (codon preference parameter), and seven (-18, -16, -15,-9, -7, -1, +6) for mRNA abundance in the flanking sequence of the initiator AUG codonrespectively, but a significantly positive correlation for both CAI and CPP at twopositions (-4 and +5), indicating that both those positions are evolutionally under thenatural selection constraint at the translational level. By site-directed mutagenesis atseven specific positions (-18, -16, -15, -9, -7, -1 and +6) for allergenic protein thathad the highest mRNA abundance in this study, its expression level decreased dramatically63.3 and 72.5% respectively, indicating the importance of those 7 positions for geneexpression. A highly positive correlation (r=0.625, P<0.01) between AUGCAI and GCcontent in the flanking sequence of the initiator AUG codon showed a more effectivehigher GC content on translation initiation efficiency. The strong preference for G orC at those 8 positions (-6, -5, -3, -2, -1, +4, +5 and +6) in the AUG context suggestedthat an important factor in modulation of the translation efficiency, as well assynonymous codon usage bias, particularly in highly expressed genes.展开更多
不能闲着的夏天,抢先跟大家预告一波,ViVi与你不见不散.QUEEN REAL TRIBUTE皇后致敬乐队上海演唱会《波西米亚狂想曲》演出场馆:上海东方艺术中心音乐厅演出时间:2019年8月30日不久前刚斩获第91届奥斯卡最佳男主角、最佳剪辑等四项大奖...不能闲着的夏天,抢先跟大家预告一波,ViVi与你不见不散.QUEEN REAL TRIBUTE皇后致敬乐队上海演唱会《波西米亚狂想曲》演出场馆:上海东方艺术中心音乐厅演出时间:2019年8月30日不久前刚斩获第91届奥斯卡最佳男主角、最佳剪辑等四项大奖的《波西米亚狂想曲》回顾了有关皇后乐队的所有的重要历程.展开更多
文摘以细菌和古菌基因组5′UTR序列作为研究对象,分析在5′UTR的3个不同阅读框架中三联体AUG的分布,发现无论是细菌还是古菌基因组都在阅读框1中有非常明显的AUG缺失(depletion)。AUG的缺失表明在起始密码子上游的AUG很可能会对基因的翻译起始产生影响。分析得知:绝大部分的AUG都是以uORF(upstream open reading frame)的形式出现的,uAUG(upstream AUG)的数量很少,特别是在阅读框1中,而且在细菌基因组的阅读框1中uAUG较多地出现在了含有SD序列的基因上游。比较发现,uAUG引导的序列在同义密码子使用上的偏好性较真正的编码序列差,这可能表明细菌和古菌在同义密码子使用上的偏好性也是决定基因准确地翻译起始的重要因素之一。
文摘The 15th Power Systems Computation Conference (PSCC'05) will take place in Liege, Belgium,August 22-26, 2005. PSCC has developed into one of the premier conferences in the area. The emphasis is on both theoretical developments as well as practical applications in the utility and manufacturing industries.
文摘This paper mainly discusses the variation of cosmic ray intensitycaused by solar flares in Mar. and Aug. 1989. Qualitative comparisonand analysis have been made for the events in Mar. and Aug. especiallyfrom the difference of the variation of cosmic ray intensity in BeijingTokoy, and Guangzhou, and the anisotropic character of cosmic rayintensity from East to West direction recorded by the scintillator telescope in Guangzhou. It is found that though the events of decreaseof cosmic ray in Mar. and Aug. both coused by solar flares, there aredifference between two events, either in the shape of the decrease of cosmic ray or in the energy distribution of the falling particles.1989 is one of the years of the nationwide joint observation for the 22’ Max. The scintillator multi-directional telescope in Guang-zhou Observatory took part in the work.
基金This work was supported by the National Natural Science Foundation of China(39870421)the Key Research Project of Zhejiang Province,China(2003C22007).
文摘The relationship between the codon usage bias, gene expression level and the AUG context(from -20 to +6 positions relative to the initiator AUG codon) was examined in 541unigene sequences of rice. A significant correlation for CAI values (codon adaptationindex) was observed at five nucleotide positions (-19, -18, -9, -4, +5), eight (-19, -18,-14, -9, -6, -4, -1, +5) for CPP (codon preference parameter), and seven (-18, -16, -15,-9, -7, -1, +6) for mRNA abundance in the flanking sequence of the initiator AUG codonrespectively, but a significantly positive correlation for both CAI and CPP at twopositions (-4 and +5), indicating that both those positions are evolutionally under thenatural selection constraint at the translational level. By site-directed mutagenesis atseven specific positions (-18, -16, -15, -9, -7, -1 and +6) for allergenic protein thathad the highest mRNA abundance in this study, its expression level decreased dramatically63.3 and 72.5% respectively, indicating the importance of those 7 positions for geneexpression. A highly positive correlation (r=0.625, P<0.01) between AUGCAI and GCcontent in the flanking sequence of the initiator AUG codon showed a more effectivehigher GC content on translation initiation efficiency. The strong preference for G orC at those 8 positions (-6, -5, -3, -2, -1, +4, +5 and +6) in the AUG context suggestedthat an important factor in modulation of the translation efficiency, as well assynonymous codon usage bias, particularly in highly expressed genes.
文摘不能闲着的夏天,抢先跟大家预告一波,ViVi与你不见不散.QUEEN REAL TRIBUTE皇后致敬乐队上海演唱会《波西米亚狂想曲》演出场馆:上海东方艺术中心音乐厅演出时间:2019年8月30日不久前刚斩获第91届奥斯卡最佳男主角、最佳剪辑等四项大奖的《波西米亚狂想曲》回顾了有关皇后乐队的所有的重要历程.
