Let （y, d, dλ） be （Rn, ｜·｜,μ）, where ｜·｜ is the Euclidean distance, μ is a nonnegative Radon measure on Rn satisfying the polynomial growth condition, or the Gauss measure metric space （Rn, ｜...Let （y, d, dλ） be （Rn, ｜·｜,μ）, where ｜·｜ is the Euclidean distance, μ is a nonnegative Radon measure on Rn satisfying the polynomial growth condition, or the Gauss measure metric space （Rn, ｜·｜,dγ）, or the space （S, d, p）, where S - Rn×R＋ is the （ax ＋ b）-group, d is the left-invariant Riemannian metric and p is the right Haar measure on S with exponential growth. In this paper, the authors introduce and establish some properties of the atomic Hardy-type spaces {Xs（Y））0〈s≤∞ and the BM0-type spaces {BM0（y, s）}0〈s≤∞. Let Hi（Y） be the known atomic Hardy space and L01（y） the subspace of f ∈ L1（Y） with integral 0. The authors prove that the dual space of Xs（Y） is SM0（Y,s） when s∈ （0, ∞）, Xs（Y） = H1（Y） when s ∈ （0, 1], and X∞（y） = L01（Y） （or L1（Y））. As applications, the authors show that if T is a linear operator bounded from H1 （Y） to L1 （Y） and from L1（y） to L1,∞（Y）, then for all r ∈ （1, ∞） and s ∈ （r, ∞], T is bounded from Xr（y） to the Lorentz space L1,8（y）, which applies to the Calderon-Zygmund operator on （Rn, ｜·｜,μ）, the imaginary powers of the 0rnstein-Uhlenbeck operator on （Rn, ｜·｜,dγ） and the spectral operator associated with the spectral multiplier on （S, d, p）. All these results generalize the corresponding results of Sweezy, Abu-Shammala and Torchinsky on Euclidean spaces.展开更多
Interaction between nucleus and cytoplasm has been focused in the field of animal embryonic development, in which study of maternal factors is required positively. b-Catenin, an important maternal factor in early embr...Interaction between nucleus and cytoplasm has been focused in the field of animal embryonic development, in which study of maternal factors is required positively. b-Catenin, an important maternal factor in early embryo-genesis, has been analyzed in its expression pattern and func-tions in this paper. We have cloned goldfish b-Catenin cDNA gene and compared it with zebrafish b-Catenin cDNA. High homology was found in cDNA and in amino acid sequences between them, 93% (2227/2384 bp) and 98.5% (768/780 aa) respectively. The expression pattern of b-Catenin by in situ hybridization and the roles of b-Catenin on embryonic de-velopment by co-injection of anti-sense RNA and reporter gene, EGFP have been investigated in the whole process of goldfish embryonic development. The results suggest that b-Catenin presents dynamic distribution, mainly locates at body axis, dorsal tissues, head and tail structures after being fertilized. The loss of b-Catenin activity would cause serious destruction of embryo in dorsal tissues and in anteroposte-rior axes, and leads embryos to die before larva get hatched.展开更多
目的探讨二项酶诱导剂5,6-二氢环戊烯-1,2-二硫杂环戊烯-3-硫酮(CPDT)对高糖环境下大鼠Müller细胞凋亡的影响及其与凋亡相关基因B淋巴细胞瘤-2(Bcl-2)和B淋巴细胞瘤-2相关X蛋白(Bax)通路的关系。方法体外培养SD大鼠的Müller细...目的探讨二项酶诱导剂5,6-二氢环戊烯-1,2-二硫杂环戊烯-3-硫酮(CPDT)对高糖环境下大鼠Müller细胞凋亡的影响及其与凋亡相关基因B淋巴细胞瘤-2(Bcl-2)和B淋巴细胞瘤-2相关X蛋白(Bax)通路的关系。方法体外培养SD大鼠的Müller细胞,采用数字表法随机分为对照组、高糖组和CPDT干预组。对照组给予25 mM DMEM完全培养基培养;高糖组给予65 mM DMEM完全培养基培养;CPDT干预组给予65 mM DMEM完全培养基和60μM CPDT培养,均培养72 h。采用双染法流式细胞仪检测各组细胞凋亡和细胞周期的情况;采用蛋白免疫印迹杂交技术检测各组细胞Bcl-2和Bax蛋白的表达情况。不同组的组间比较采用单因素方差分析,当差异有统计学意义时,进一步采用LSD法两两比较。结果流式细胞仪检测的结果显示,高糖组的细胞凋亡率为(17.6±3.16)%;对照组的细胞凋亡率为(8.25±0.26)%;CPDT干预组的细胞凋亡率为(13.5±2.25)%,且三组细胞凋亡率间,差异有统计学意义(F=13.48,P<0.05)。蛋白免疫印迹杂交技术检测的结果显示,高糖组Bcl-2蛋白表达量较对照组降低,Bax蛋白表达量较对照组升高,且差异有统计学意义(t=150.38,234.46;P<0.05)。CPDT干预组Bcl-2蛋白表达量较高糖组升高,Bax蛋白表达量较高糖组降低,且差异有统计学意义(t=108.03,36.33;P<0.05)。结论高糖可通过上调Bax,下调Bcl-2表达,降低Bcl-2/Bax的比值,促进细胞凋亡。CPDT可增加高糖影响下的大鼠Müller细胞活性、Bcl-2的表达及Bcl-2/Bax的比值,抑制Bax的表达和高糖引起的大鼠Müller细胞的凋亡,从而起到保护高糖环境中Müller细胞受损伤的作用。展开更多
基金Supported by National Natural Science Foundation of China （Grant No. 10871025）
文摘Let （y, d, dλ） be （Rn, ｜·｜,μ）, where ｜·｜ is the Euclidean distance, μ is a nonnegative Radon measure on Rn satisfying the polynomial growth condition, or the Gauss measure metric space （Rn, ｜·｜,dγ）, or the space （S, d, p）, where S - Rn×R＋ is the （ax ＋ b）-group, d is the left-invariant Riemannian metric and p is the right Haar measure on S with exponential growth. In this paper, the authors introduce and establish some properties of the atomic Hardy-type spaces {Xs（Y））0〈s≤∞ and the BM0-type spaces {BM0（y, s）}0〈s≤∞. Let Hi（Y） be the known atomic Hardy space and L01（y） the subspace of f ∈ L1（Y） with integral 0. The authors prove that the dual space of Xs（Y） is SM0（Y,s） when s∈ （0, ∞）, Xs（Y） = H1（Y） when s ∈ （0, 1], and X∞（y） = L01（Y） （or L1（Y））. As applications, the authors show that if T is a linear operator bounded from H1 （Y） to L1 （Y） and from L1（y） to L1,∞（Y）, then for all r ∈ （1, ∞） and s ∈ （r, ∞], T is bounded from Xr（y） to the Lorentz space L1,8（y）, which applies to the Calderon-Zygmund operator on （Rn, ｜·｜,μ）, the imaginary powers of the 0rnstein-Uhlenbeck operator on （Rn, ｜·｜,dγ） and the spectral operator associated with the spectral multiplier on （S, d, p）. All these results generalize the corresponding results of Sweezy, Abu-Shammala and Torchinsky on Euclidean spaces.
文摘Interaction between nucleus and cytoplasm has been focused in the field of animal embryonic development, in which study of maternal factors is required positively. b-Catenin, an important maternal factor in early embryo-genesis, has been analyzed in its expression pattern and func-tions in this paper. We have cloned goldfish b-Catenin cDNA gene and compared it with zebrafish b-Catenin cDNA. High homology was found in cDNA and in amino acid sequences between them, 93% (2227/2384 bp) and 98.5% (768/780 aa) respectively. The expression pattern of b-Catenin by in situ hybridization and the roles of b-Catenin on embryonic de-velopment by co-injection of anti-sense RNA and reporter gene, EGFP have been investigated in the whole process of goldfish embryonic development. The results suggest that b-Catenin presents dynamic distribution, mainly locates at body axis, dorsal tissues, head and tail structures after being fertilized. The loss of b-Catenin activity would cause serious destruction of embryo in dorsal tissues and in anteroposte-rior axes, and leads embryos to die before larva get hatched.
文摘目的探讨二项酶诱导剂5,6-二氢环戊烯-1,2-二硫杂环戊烯-3-硫酮(CPDT)对高糖环境下大鼠Müller细胞凋亡的影响及其与凋亡相关基因B淋巴细胞瘤-2(Bcl-2)和B淋巴细胞瘤-2相关X蛋白(Bax)通路的关系。方法体外培养SD大鼠的Müller细胞,采用数字表法随机分为对照组、高糖组和CPDT干预组。对照组给予25 mM DMEM完全培养基培养;高糖组给予65 mM DMEM完全培养基培养;CPDT干预组给予65 mM DMEM完全培养基和60μM CPDT培养,均培养72 h。采用双染法流式细胞仪检测各组细胞凋亡和细胞周期的情况;采用蛋白免疫印迹杂交技术检测各组细胞Bcl-2和Bax蛋白的表达情况。不同组的组间比较采用单因素方差分析,当差异有统计学意义时,进一步采用LSD法两两比较。结果流式细胞仪检测的结果显示,高糖组的细胞凋亡率为(17.6±3.16)%;对照组的细胞凋亡率为(8.25±0.26)%;CPDT干预组的细胞凋亡率为(13.5±2.25)%,且三组细胞凋亡率间,差异有统计学意义(F=13.48,P<0.05)。蛋白免疫印迹杂交技术检测的结果显示,高糖组Bcl-2蛋白表达量较对照组降低,Bax蛋白表达量较对照组升高,且差异有统计学意义(t=150.38,234.46;P<0.05)。CPDT干预组Bcl-2蛋白表达量较高糖组升高,Bax蛋白表达量较高糖组降低,且差异有统计学意义(t=108.03,36.33;P<0.05)。结论高糖可通过上调Bax,下调Bcl-2表达,降低Bcl-2/Bax的比值,促进细胞凋亡。CPDT可增加高糖影响下的大鼠Müller细胞活性、Bcl-2的表达及Bcl-2/Bax的比值,抑制Bax的表达和高糖引起的大鼠Müller细胞的凋亡,从而起到保护高糖环境中Müller细胞受损伤的作用。
