Soil erosion and the subsequent sedimentation are serious environmental problems.Improper land management coupled with intense rainfall has complicated the problem in the Ethiopian highlands.Consequently,soil loss cos...Soil erosion and the subsequent sedimentation are serious environmental problems.Improper land management coupled with intense rainfall has complicated the problem in the Ethiopian highlands.Consequently,soil loss costs a profound amount of the national GDP and deters land productivity.Quantifying soil loss and prioritizing areas for conservation is imperative for proper planning and resource management.Therefore,this study aimed at estimating the mean annual soil loss rate of Gilgel Abay watershed which has critical implications on Lake Tana and Abbay River(Upper Blue Nile River),using RUSLE and morphometric analysis methods.Datasets including rainfall,soil,Digital Elevation Model and land use/land cover were used to generate important parameters required for the soil loss estimation.Data collected through field observation,consultation with experts and document analysis were used for validation purposes.The results of the study reveal that the watershed experienced an average soil loss rate of 39.8 t ha^(-1)yr^(-1).Subsequently,nearly 25%(37,038 ha)of the watershed is threatened by moderate to very severe soil loss.Among the four sub watersheds,sub watershed 3(S3)and sub watershed 4(S4),which consist of about 65%(105,000 ha)of the total area of the study watershed,experienced an annual soil loss rate of over 30 t ha^(-1)yr^(-1).The amount of soil loss that the study watershed has experienced is above the soil loss tolerance level of Ethiopia(2-18 t ha^(-1)yr^(-1))and the tropical region(10 t ha^(-1)yr^(-1)).Similarly,the results of morphometric analysis showed that the studied watershed has S-shaped hypsometric curve,an indication for maturity stage of landscape development.The studied watershed is also characterized by a non-circular shaped drainage with rugged and dissected topography that contributes to high flash flood,peak discharge and incidence of soil loss.The result of both models signified that S3 and S4 are highly susceptible to soil erosion.Therefore,these two sub watersheds need priority for soil and water conservation(SWC)measures.Furthermore,the study demonstrated the importance of the integration of different models in the identification of soil erosion prone areas and prioritization for the proper implementation of SWC measures.展开更多
CRISPR genome editing utilizes Cas9 nuclease and single guide RNA(sgRNA),which directs the nuclease to a specific site in the genome and makes a double-stranded break(DSB).Design of sgRNA for CRISPR-Cas targeting,and ...CRISPR genome editing utilizes Cas9 nuclease and single guide RNA(sgRNA),which directs the nuclease to a specific site in the genome and makes a double-stranded break(DSB).Design of sgRNA for CRISPR-Cas targeting,and to promote CRISPR adaptation,uses a regulatory mechanism that ensures maximum CRISPR-Cas9 system functions when a bacterial population is at highest risk of phage infection.Acinetobacter baumannii is the most regularly identified gram-negative bacterium infecting patients.Recent reports have demonstrated that the extent of diseases caused by A.baumannii is expanding and,in a few cases,now surpasses the quantity of infections caused by P.aeruginosa.Most Acinetobacter strains possess biofilm-forming ability,which plays a major role in virulence and drug resistance.Biofilm bacteria use quorum sensing,a cell-to-cell communication process,to activate gene expression.Many genes are involved in biofilm formation and the mechanism to disrupt the biofilm network is still not clearly understood.In this study,we performed in silico gene editing to exploit the AbaI gene,responsible for biofilm formation.The study explored different tools available for genome editing to create gene knockouts,selecting the A.baumannii AbaI gene as a target.展开更多
文摘Soil erosion and the subsequent sedimentation are serious environmental problems.Improper land management coupled with intense rainfall has complicated the problem in the Ethiopian highlands.Consequently,soil loss costs a profound amount of the national GDP and deters land productivity.Quantifying soil loss and prioritizing areas for conservation is imperative for proper planning and resource management.Therefore,this study aimed at estimating the mean annual soil loss rate of Gilgel Abay watershed which has critical implications on Lake Tana and Abbay River(Upper Blue Nile River),using RUSLE and morphometric analysis methods.Datasets including rainfall,soil,Digital Elevation Model and land use/land cover were used to generate important parameters required for the soil loss estimation.Data collected through field observation,consultation with experts and document analysis were used for validation purposes.The results of the study reveal that the watershed experienced an average soil loss rate of 39.8 t ha^(-1)yr^(-1).Subsequently,nearly 25%(37,038 ha)of the watershed is threatened by moderate to very severe soil loss.Among the four sub watersheds,sub watershed 3(S3)and sub watershed 4(S4),which consist of about 65%(105,000 ha)of the total area of the study watershed,experienced an annual soil loss rate of over 30 t ha^(-1)yr^(-1).The amount of soil loss that the study watershed has experienced is above the soil loss tolerance level of Ethiopia(2-18 t ha^(-1)yr^(-1))and the tropical region(10 t ha^(-1)yr^(-1)).Similarly,the results of morphometric analysis showed that the studied watershed has S-shaped hypsometric curve,an indication for maturity stage of landscape development.The studied watershed is also characterized by a non-circular shaped drainage with rugged and dissected topography that contributes to high flash flood,peak discharge and incidence of soil loss.The result of both models signified that S3 and S4 are highly susceptible to soil erosion.Therefore,these two sub watersheds need priority for soil and water conservation(SWC)measures.Furthermore,the study demonstrated the importance of the integration of different models in the identification of soil erosion prone areas and prioritization for the proper implementation of SWC measures.
文摘CRISPR genome editing utilizes Cas9 nuclease and single guide RNA(sgRNA),which directs the nuclease to a specific site in the genome and makes a double-stranded break(DSB).Design of sgRNA for CRISPR-Cas targeting,and to promote CRISPR adaptation,uses a regulatory mechanism that ensures maximum CRISPR-Cas9 system functions when a bacterial population is at highest risk of phage infection.Acinetobacter baumannii is the most regularly identified gram-negative bacterium infecting patients.Recent reports have demonstrated that the extent of diseases caused by A.baumannii is expanding and,in a few cases,now surpasses the quantity of infections caused by P.aeruginosa.Most Acinetobacter strains possess biofilm-forming ability,which plays a major role in virulence and drug resistance.Biofilm bacteria use quorum sensing,a cell-to-cell communication process,to activate gene expression.Many genes are involved in biofilm formation and the mechanism to disrupt the biofilm network is still not clearly understood.In this study,we performed in silico gene editing to exploit the AbaI gene,responsible for biofilm formation.The study explored different tools available for genome editing to create gene knockouts,selecting the A.baumannii AbaI gene as a target.
