Study on Intervention of Blood Sugar and Blood Lipid in Diabetic Mice by Abrus Cantoniensis and Armeniaca mume Sieb and Their Compatibility

Objective:The study aims to explore the interventional effect of Abrus cantonensis and Armeniaca mume Sieb and their compatibility on blood glucose and blood lipid in diabetic mice and to explore the optimal ratio of efficacy of their compatibility.Methods:SPF Kunming male mice were fed with high sugar and high fat diet for 6 weeks and then intraperitoneally injected with STZ solution to establish a diabetes model.The successfully modeled mice were randomly divided into 6 groups according to their body weight:model group,Abrus Cantoniensis group,Armeniaca mume Sieb group,as well as Abrus Cantoniensis and Armeniaca mume Sieb group divided into 1:2,1:1 and 2:1 groups according to volume ratio with 10 mice in each group.Another 10 healthy mice were taken as blank group and intraperitoneally injected with sodium citrate buffer.The corresponding solution was given intragastric administration by body mass once a day for 20 days.After the last administration,fasting was performed for 12 hours.Serum was collected by the method of eyeball blood collection to determine the contents of fasting blood glucose,total cholesterol Triacylglycerol,triglyceride,low density lipoprotein and high-density lipoprotein.Results:Compared with Abrus Cantoniensis and Armeniaca mume Sieb groups,Glu,TG,TC and LDL-C of the three compatibility groups significantly decreased,with the most significant decrease in the compatibility group 1:2,while there was no significant difference in HDL-C among all groups.Conclusion:Abrus Cantoniensis and Armeniaca mume Sieb and their combination have synergistic effects on the improvement of blood glucose and lipid levels in mice with high glucose and high fat combined with STZ-induced diabetes mellitus model.Among all the experimental groups,the compatibility 1:2 group had the best effect.

Antibacterial activity of the ethyl acetate part of Abrus cantoniensis against Staphylococcus aureus

Objective:The aim of this work was to measure the antibacterial activity(against Escherichia coli and Staphylococcus aureus[S.aureus])of the ethyl acetate part of Abrus cantoniensis and assess their potential as medicines.Methods:The experiment was divided into four groups:negative control group[with Mueller-Hinton broth(MHB)],positive control group(with 75%ethanol),blank group(with MHB)and test group(with the ethyl acetate part of Abrus cantoniensis).The antibacterial activities of the extracts were evaluated by the Oxford cup assay and minimum inhibitory concentration(MIC).Time-kill curve experiments,scanning electron microscopy,the content of DNA,RNA and protein were used to study the antibacterial mechanism of the ethyl acetate extract part on the growth and viability of S.aureus.The study procedures were approved by the Animal Care and Use Committee of Xi’an Jiaotong University(approval No.XJTULAC2016-412)on January 22,2016.Results:The ethyl acetate part of Abrus cantoniensis extract exhibited the highest inhibitory activity against the growth of S.aureus with an inhibition zone diameter of 16.4mm and MIC value of 0.5mg/mL.The general activity range of the ethyl acetate part,determined using a time-killing curve,was found to be 0.5mg/mL to 40mg/mL(MIC to 80
MIC).Changes in the scanning electron microscopy images and of DNA,RNA and proteins of S.aureus indicated possible mechanisms of the inhibitory activity of the ethyl acetate part.Conclusion:The ethyl acetate part of Abrus cantoniensis damaged bacterial cell structures,which results in protoplasm leakage,and eventually bacterial cell death.

桉—草、桉—药复合经营模式的经济效益分析与示范推广

营建了桉—山毛豆(Tephrosia candida)、桉—鸡骨草(Abrus cantoniensis)复合经营模式,分析其经济效益,为桉树(Eucalyptus spp.)人工林可持续发展做示范推广。桉树人工林采用复合经营技术能有效地解决桉树人工林经营过程中出现的生态问题。推广应用桉树复合经营模式对提高林地综合生产能力、促进林农增收、改善林地生态环境有重要的现实意义。