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高原世居藏族α、β珠蛋白编码基因的克隆与测序 被引量:2
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作者 董宏彬 洪欣 +4 位作者 聂鸿靖 肖忠海 何贵祥 黑沙汉 尹昭云 《中国应用生理学杂志》 CAS CSCD 北大核心 2005年第2期196-199,共4页
目的:通过对高原世居藏族α、β珠蛋白编码基因的分析,探讨藏族Hb高氧亲合力的分子机制。方法:高原现场采集健康成年男性藏族人骨髓样品,提取总RNA,通过逆转录聚合酶链反应(RT PCR)获得人α和β珠蛋白的cDNA,与PGEM-TEasy质粒连接后,将... 目的:通过对高原世居藏族α、β珠蛋白编码基因的分析,探讨藏族Hb高氧亲合力的分子机制。方法:高原现场采集健康成年男性藏族人骨髓样品,提取总RNA,通过逆转录聚合酶链反应(RT PCR)获得人α和β珠蛋白的cDNA,与PGEM-TEasy质粒连接后,将α和β珠蛋白的cDNA转化JM109大肠杆菌中扩增培养,经酶切鉴定后测序,结果与NCBI数据库进行同源性比较。结果:藏族人α珠蛋白的cDNA与NCBI数据库登录的人cDNA序列相同,没有突变位点。一例藏族人β珠蛋白143位密码子发生了氧亲和力增高的碱基突变(CAC>CGC),其对应的氨基酸由His变为Arg(即HbAbruzzo)。结论:藏族人高氧亲和力变种的发现,为今后高原低氧适应相关基因的研究提供了线索。 展开更多
关键词 高原 藏族 珠蛋白 基因 HB abruzzo
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Capturing the fungal community associated with conventional and organic Trebbiano Abruzzese grapes and its influence on wine characteristics
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作者 Alessio Pio Rossetti Giorgia Perpetuini +5 位作者 Noemi Battistelli Camillo Zulli Giuseppe Arfelli Giovanna Suzzi Angelo Cichelli Rosanna Tofalo 《Food Bioscience》 SCIE 2023年第2期558-565,共8页
In this study,the influence of conventional and organic managements on Vitis vinifera cv.Trebbiano Abruzzese on fungal community was investigated.Moreover,spontaneous vinifications were performed,and wines were charac... In this study,the influence of conventional and organic managements on Vitis vinifera cv.Trebbiano Abruzzese on fungal community was investigated.Moreover,spontaneous vinifications were performed,and wines were characterized for some oenological parameters and aroma profiles.A core of 8 genera was detected(Zygosaccharomyces spp.,Cladosporium spp.,Botrytis spp.,Hanseniaspora spp.,Pichia spp.,Alternaria spp.,Candida spp.,Aureobasidium spp.),while some genera were unique for each grape sample.Saccharomyces spp.and Mucor spp.were present only on conventional grapes,while Penicillium spp.and Aspergillus spp.only on organic ones.At the species level Aureobasidium pullulans,Cladosporium cladosporioides and Pichia terricola dominated in both samples with varying abundance depending on the vineyard management.The fermentation performed with conventional grapes resulted in a faster rate than that performed with organic ones.No significative differences were observed for the oenological parameters analyzed with the only exception of ethanol content which was higher in conventional wines than in organic ones:11.57±0.10 g/L and 10.51±0.09 g/L,respectively.Conventional wines were characterized by the presence of aldehydes,while organic ones by terpenes and β-damascenone.The obtained results suggested an effect of vineyard practices on grape fungal community which could in turn influence some characteristics of Trebbiano d’Abruzzo wines. 展开更多
关键词 Trebbiano d’abruzzo wine Organic management Conventional management Fungal community Aroma compounds
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