Ameliorating effects of constituents from Cortex Acanthopanacis Radicis on memory impairment in mice induced by scopolamine

OBJECTIVE:To investigate the effects of active constituents extracted from Cortex Acanthopanacis Radicison improving the impaired memory in mice models.METHODS:The mice models of memory impairment were established using scopolamine.Ameliorating effects of the fractions and constituents on scopolamine-induced memory impairment in vivo were investigated using passive avoidance and Morris water-maze task tests,and their anti-acetylcholinesterase(AChE)and antioxidant activities in vitro examined.The isolation of constituents was performed by chromatographic methods and their structures were identified on the basis of instrumental analysis.RESULTS:Among the fractions tested,ethylacetate fraction exhibited the anti-AChE activity(25.83%±0.23%)properly and excellent 2,2-diphenyl-1-picrylhydrazyl(DPPH)radical and superoxide anion scavenging capacity(87.50%±0.83%and 60.22%±0.43%,respectively).However,the methylene chloride fraction was much more active than the ethylacetate fraction in the passive avoidance task test(167.5%increase of step-through latency time)and Morris water-maze task test(33.3%decrease of es-cape latency time).Four constituents,β-sitosterol,stigmasterol,sesamin,and hyperin were isolated from the methylene chloride fraction,among them,hyperin showed anti-acetylcholinesterase and anti-oxidant activities remarkably.Moreover,hyperin exerted a potent effect(146±38)s on memory improvement in terms of passive avoidance task test compared with the reference compound tacrine(162±43)s at a dose of 2.5 mg/kg.CONCLUSION:Hyperin,a flavonoid glucoside isolated from Cortex Acanthopanacis Radicis,inhibited AChE activity and potently ameliorated scopolamine-induced memory impairment,and its action may be partially mediated by the acetylcholine-enhancing cholinergic nervous system.

刺五加不同药用部位中槲皮素鼠李糖苷的含量比较

[目的]测定刺五加不同药用部位中槲皮素鼠李糖苷含量,并对结果进行比较,为刺五加合理利用及质量评价提供科学依据。[方法]采用高效液相色谱法测定刺五加的根、茎、叶、花和果中槲皮素鼠李糖苷含量。色谱条件为:大连江申Nucleosil C18(4.6 mm×150 mm,5μm)色谱柱,流动相为无水甲醇-水(40∶60,V/V),流速为1.1 ml/min,检测波长为370 nm,柱温为30℃,进样量为10μl。[结果]刺五加各药用部位中槲皮素鼠李糖苷的含量大小顺序为花蕾>叶>果>茎>根。其中,花蕾中槲皮素鼠李糖苷含量最高,为4.43%;其次为叶(0.61%);根中的最低,为0.01%。[结论]该方法操作简便、快速、准确,可用于刺五加不同部位中的槲皮素鼠李糖苷的含量比较。

紫五加根茎中总黄酮含量的测定

以芦丁为对照品,采用紫外分光光度法,于420 nm波长处测定吸光度,计算紫五加(Acanthopanacis senticosi Radix)根茎中总黄酮的含量。结果表明,在4~28μg/m L的范围内,芦丁含量与吸光度有良好的线性关系,加标回收率为100.25%(n=4,RSD=0.92%),紫五加根茎提取液中总黄酮平均含量29.62μg/m L。该方法简便、快速、准确、灵敏,可作为紫五加根茎中总黄酮含量的测定方法。