温度和盐度对刺枝鱼栖苔(Acanthophora spicifera)(红藻门,松节藻科)生长及其几种光合色素的影响

通过正交试验研究了温度和盐度对刺枝鱼栖苔(Acanthophora spicifera)生长及其光合色素的影响,以探明刺枝鱼栖苔生长的最适温度及盐度条件。结果表明,刺枝鱼栖苔生长的适宜温度范围为20—30℃,最适温度为25℃,在此温度条件下其相对生长率(RGR)最大为4.33%;适宜盐度范围为15—35,最适盐度范围为20—25,随盐度的继续升高,刺枝鱼栖苔RGR呈下降趋势。温度和盐度对刺枝鱼栖苔光合色素(chl a、Car、PE和PC)的影响显著,高盐胁迫能明显降低刺枝鱼栖苔光合色素的含量,而高温能促进其藻胆蛋白的合成与积累。

A new ketosteroid from red alga Acanthophora spicifera

A new ketosteroid, along with six known steroids, was isolated from the ethanolic extracts of red alga Acanthophora spicifera （Vahl.） Boergesen. The structures, identified using chemical and spectroscopic methods including 2D NMR, were： （1） 22-hydroxy-5α-eholest-3,6-dione, （2） 6-hydroxycholest-4-ene-3-one, （3） cholest-4-ene-3,6-dione, （4） cholest-5-ene-3β-ol, （5） 5u-cholestane-3,6-dione, （6） β-Sitosterol and （7） Saringosterol. The MTT method was used to test the cytotoxicity of the compounds against the human cancer cell lines, HCT-8, Bel-7402, BGC-823, A549 and HELA. Compounds 1, 2, 3 and 5 showed moderate cytotoxic activity against human cancer cell lines.

The protective effect of dietary flavonoid fraction from Acanthophora spicifera on streptozotocin induced oxidative stress in diabetic rats

The present investigation was considered in arraying of antidiabetic and antioxidant activity from dietary flavonoid loaded fraction of Acanthophora spicifera(A.spicifera,Family:Rhodomelaceae)on streptozotocin(STZ)induced oxidative stress rats.The testings were acted upon male rats,which were alienated into five groups:control group,diabetic group(single dose of 65 mg/kg,streptozotocin(STZ)i.p.),diabetic with insulin(6 IU),and diabetic with flavonoid rich fraction groups(FRF)at 50 and 100 mg/kg body weight,given orally for 21 days.The blood glucose level was determined at different week intermissions.The antioxidant consequences of FRF on STZ-induced diabetic rats were determined by the estimations of the oxidative stress marker like malonyldialdehyde and antioxidant enzymes such as superoxide dismutase,catalase and glutathione in tissue homogenates of heart,liver and kidney.FRF treatment of diabetic rats significantly(P<0.05)diminishes the blood glucose altitudes to normal in contrast with diabetic rats.However,FRF administration,significantly decreased the malonyldialdehyde(MDA)and increased the activities of superoxide dismutase(SOD),catalase(CAT)and glutathione levels(GSH)in diabetic rats.The outcome designates that FRF fraction from red algae A.spicifera was potent anti diabetic and antioxidant asset against STZ induced diabetes and oxidative tissue breakups.©2016 Beijing Academy of Food Sciences.Production and hosting by Elsevier B.V.All rights reserved.

南中国海海藻刺状鱼栖苔的化学成分研究(I)

从采自南中国海红藻刺状鱼栖苔的乙醇提取物中分离出一个黄酮类化合物，经ＩＲ，ＵＶ，ＦＡＢＭＳ，１Ｈ－１ＨＣＯＳＹ，１３Ｃ－１ＨＣＯＳＹ，ＨＭＢＣ等现代波谱技术确定其结构为５，７－二羟基－２－对羟基苯基－３－Ｏ－（６－Ｏ－对羟基肉桂酰基－β－Ｄ－吡喃葡萄糖基）－４Ｈ－１－苯并吡喃－４－酮，并对碳信号进行了明确归属．这是首次从红藻中分离出黄酮类化合物．

南中国海海藻刺状鱼栖苔的化学成分(Ⅲ)

采自南中国海刺状鱼栖苔的乙醇提取物经多种柱层析分离并鉴定出四种化合物,分别为:(24S) 5 烯 3β 谷甾醇、对羟基苯甲酸、黄酮类化合物槲皮素及类黄酮化合物(-) 表儿茶精.其结构通过各种波谱技术如IR,GC MS、FABMS、1HNMR、13CNMR(DEPT)、1H 1HCOSY和HMBC等加以证实.这些化合物均是首次从刺状鱼栖苔中检测到.其中槲皮素和(-)

红藻刺状鱼栖苔中具有生物活性的甾体类化合物

目的:对红藻刺状鱼栖苔Acanthophora spicifera中的甾体类化学成分进行研究,寻找具有生物活性的先导化合物。方法:利用正相和反相柱色谱、Sephadex LH-20柱色谱、反相HPLC以及重结晶等手段进行分离纯化,借助MS,IR,1D和2D NMR等方法鉴定结构;通过MTT法对分离鉴定的单体化合物在HCT-8,Bel-7402,BGC-823,A549和HELA细胞株上进行细胞毒活性测试;通过MTT法对单体化合物在犬血管平滑肌细胞模型上进行增殖抑制活性测试。结果:从刺状鱼栖苔中分离得到6个甾体类化合物,分别鉴定为6-羟基-胆甾-4-烯-3-酮(1)、胆甾-4-烯-3,6-二酮(2)、胆甾-5-烯-3β-醇(3)、5α-胆甾-3,6-二酮(4)、β-谷甾醇(5)和24-羟基-24-乙烯基-胆甾醇(6)。结论:其中化合物1,2,3,5为首次从该海藻中分离鉴定;化合物1,2,4表现出一定细胞毒活性。