Natural variations and geographical distributions of seed carotenoids and chlorophylls in 1167 Chinese soybean accessions

Understanding the composition and contents of carotenoids in various soybean seed accessions is important for their nutritional assessment.This study investigated the variability in the concentrations of carotenoids and chlorophylls and revealed their associations with other nutritional quality traits in a genetically diverse set of Chinese soybean accessions comprised of cultivars and landraces.Genotype,planting year,accession type,seed cotyledon color,and ecoregion of origin significantly influenced the accumulation of carotenoids and chlorophylls.The mean total carotenoid content was in the range of 8.15–14.72μg g–1 across the ecoregions.The total carotenoid content was 1.2-fold higher in the landraces than in the cultivars.Soybeans with green cotyledons had higher contents of carotenoids and chlorophylls than those with yellow cotyledons.Remarkably,lutein was the most abundant carotenoid in all the germplasms,ranging from 1.35–37.44μg g–1.Carotenoids and chlorophylls showed significant correlations with other quality traits,which will help to set breeding strategies for enhancing soybean carotenoids without affecting the other components.Collectively,our results demonstrate that carotenoids are adequately accumulated in soybean seeds,however,they are strongly influenced by genetic factors,accession type,and germplasm origin.We identified novel germplasms with the highest total carotenoid contents across the various ecoregions of China that could serve as the genetic materials for soybean carotenoid breeding programs,and thereby as the raw materials for food sectors,pharmaceuticals,and the cosmetic industry.

Agro-Morphological Characterization of a Collection of Fonio [Digitaria exilis (Kippist.) Stapf.] Accessions from Burkina Faso

Considered as the oldest cereal, fonio (Digitaria exilis Stapf.) is grown in West Africa for its therapeutic virtues, its nutritional and organoleptic qualities. It plays an important socio-cultural and economic role for the populations of western Burkina Faso. However, very few studies on the genetic diversity of fonio have been carried out. This study aims to contribute to a better knowledge of the diversity of Digitaria exilis in Burkina Faso using agro-morphological descriptors. Thus, 60 accessions of fonio collected from western Burkina Faso were characterized using 21 agro-morphological characters according to an Alpha Latice design with three replications. The study showed morphological variability for most of the qualitative and quantitative characters studied. Principal component analysis (PCA) explained 56.07% of the variability. Hierarchical cluster analysis splits the accessions of the collection into four clusters using seven characters. These characters can constitute basic criteria for differentiating fonio accessions in Burkina Faso. The accessions of Group II have a long cycle and high production of grain, while the accessions of Group IV have a short cycle and low production of grain. The groups identified can serve as a starting point for fonio breeding in Burkina Faso.

Introduction of PIC/S Accession Procedure and Suggestions for Establishing a Unified Quality Management System in China

Objective To introduce the accession procedure and evaluation of PIC/S,and help relevant departments in China to understand the requirements of PIC/S in detail,and to promote the process of China’s entry into PIC/S.Methods The procedures and steps of joining PIC/S,the evaluation process of PIC/S,and the experience of several countries in joining PIC/S were introduced and analyzed,which could help the relevant personnel in China understand the specific contents.Results and Conclusion According to the requirements of PIC/S,it is necessary to establish a unified GMP quality management system.

Screening and evaluation for antibiosis resistance of the spring wheat accessions to the grain aphid,Sitobion miscanthi(Takahashi)(Hemiptera:Aphididae)

Resistant cultivar deployment is an effective method for cereal aphid management.Under greenhouse conditions,preliminary antibiosis resistance screening was conducted on 114 Ethiopian and 22 Chinese spring wheat accessions.After performing a bioassay to determine antibiosis resistance,aphid feeding behaviour and phenolic acid content analyses were performed on the aphid resistant wheat accessions by electrical penetration graph(EPG)and high performance liquid chromatography(HPLC),respectively.Among the wheat accessions,two high resistances,27moderate-resistances,and 35 low-resistances to Sitobion miscanthi were identified.The antibiosis resistance test showed prolonged pre-adult and pre-reproductive periods,shorter reproductive periods,lower fecundity,an intrinsic rate(rm)of increase,and a finite rate(λ)of increase of S.miscanthi on Lunxuan 145,Wane,Lunxuan 6,204511,Lunxuan 103and 5215 than those on the aphid-susceptible accession Beijing 837.The changes for the parameters of aphid feeding behaviour,including spending a longer time in the penetration and phloem salivation phases and less time in the phloem sap-feeding phase on the resistant wheat accessions,indicated that the aphid resistance may occur during the phloem phase and may be due to difficulties in the mechanical probing of the mesophyll cells.Additionally,the HPLC analysis showed higher contents of:1)ferulic acid in Lunxuan 145,Lunxuan 103 and Lunxuan 6;2)p-coumaric acid in Lunxuan145;3)vanillic acid in Lunxuan 145,Wane and Lunxuan 6;4)syringic acid in Lunxuan 103;and 5)caffeic acid in 5215.The contents of some phenolic acids within wheat leaves,such as p-courmaric acid and vanillic acid showed significant positive correlation with the duration of aphid development,but negative correlation with the aphid fecundity.The concentrations of these acids may be the causes of antibiosis resistance to S.miscanthi.The identification of grain aphid-resistant wheat accessions in our study will be helpful in future breeding program for pest control.

Greenhouse and Field Evaluation of Selected Sweetpotato (<i>Ipomoea batatas</i>(L.) LAM) Accessions for Drought Tolerance in South Africa

Sweetpotato is a major staple food in the world. It is a good source of carbohydrates, vitamin A, vitamin C and protein. The maximum production potential of the crop is being hampered by severe drought which ravages most parts of Africa. The main aim of this study therefore was to screen accessions of sweetpotato for drought tolerance in a quick screening method, followed by field screening with a view to identify accessions that can perform well under water stress conditions. Fifty sweetpotato accessions consisting of cultivars and breeding lines collected from the ARC-VOPI gene bank were planted for drought screening in the glass house for six weeks during which water was withheld to induce stress. Observations were made on number of dead plants and days to wilting point. The results were analyzed and 12 best performing accessions were selected for field trials. The field trial was carried out in Lwamondo, Limpopo province, a drought prone area in South Africa, under rain-fed conditions. The best performing accessions were Zapallo, Tacna, Ejumula, 2004-9-2 and Ndou.

REPORT OF THE WORKING PARTY ON THE ACCESSION OF CHINA (Ⅳ)

2206. The representative of China confirmed that China also agreed to raise the limit within which investments in motor vehicle manufacturing could be approved at provincial government level only, from the current level of US$30 million, to US$60 million one year after accession, US$90 million two years after accession, and US$150 million four years after accession. The Working Party took note of this commitment. 207. With respect to the manufacture of motor vehicle engines, the representative of China ...

Accession of the people’s republic of china

The Ministerial Conference Decision of 10 NOV.2001 Having regard to paragraph 2 of Article Ⅻ and paragraph 1 of Article Ⅸ of the Marrakesh Agreement Establishing the World Trade Organization, and the Decision—Making Procedures under Articles Ⅸ and Ⅻ of the Marrakesh Agreement Establishing the World Trade Organization agreed by the General Council (WT/L/93). Taking note of the application 0f the People's Republic of China for accession to the Marrakesh Agreement Establishing the World Trade Organiza-

REPORT OF THE WORKING PARTY ON THE ACCESSION OF CHINA (Ⅲ)

119. The representative of China confirmed that the role of sub-national bodies would be limited to purely administrative operations, such as receiving applications from endusers and forwarding them to the central authority; receiving queries and transmitting these to the central authority; reporting on allocation and reallocation decisions made by the central authority and providing information regarding such allocations and reallocations upon request; checking the information in the applications to ve...

Selection of Sesame Accessions(Sesamum indicum L.)in Thua Thien Hue Province,Vietnam

The main objective of this study was to select the best accessions for use as potential breeding sources in Thua Thien Hue province.A total of 16 accessions were used in this study,of which three accessions from Ho Chi Minh city in Vietnam,four accessions supplied by Japan,six accessions collected from China,two accessions provided by the United States and one MèVàng local accession(control)from Thua Thien Hue.The field experiment was conducted from August 2016 to November 2016 and laid out in a random complete block design(RCBD)for three replications at Huong Long ward,Hue city,Thua Thien Hue province.Each accession was observed 10 plants per replication.The size of each plot was 10 m2 and the spacing of 25 cm×10 cm was applied.The results showed that experiment accessions can grow under Thua Thien Hue conditions.Of those,the actual yield of VV12,VDM21,VH12,VDM50,VH01 and VDM45 were higher than the other accessions with 1.65,1.64,1.55,1.54,1.52 and 1.45 tons/ha,respectively.Oil content of these six accessions was above 50%.Therefore,VV12,VDM21,VH12,VDM50,VH01 and VDM45 can be used for breeding and cultivation under local condition.

Local Taxonomy and Diversity of Chadian Sorghum [<i>Sorghum bicolor</i>(L.) Moench] Accessions

The integration of local plant genetic resources into the breeding program is an asset for research. The objective of this study is to assess the diversity and analyze the management practices of cultivated sorghum. A questionnaire following a participatory approach was submitted to 675 producers in 45 villages. The cultivars collected were characterized using 9 qualitative descriptors. In total, 151 accessions and 191 names were inventoried. On average, 25.17 accessions per department and 3.36 accessions per village. Local taxonomy is based on many criteria expressed in different local languages. The most common method of naming is that to the word sorghum is added either the color of the grain, the precocity, or any element deemed relevant by the farmers. Significant variability in grain and panicle traits was observed within the collection, except the color of the endosperm. The rate of diversity loss is 11.52% and red grain sorghums are the most threatened with extinction. Five accessions groups are differentiated by the presence or absence of awns, the color of glumes, and amount of grain covered by glumes, grain plumpness, and form. Based on productivity, precocity, plant size, seed shape, and color, the farmers identified seven promising accessions. This potential will be preserved and developed in the sorghum varietal improvement program in Chad.

Agromophological Characterisation of Amaranth Accessions from Malawi

Five amaranth (Amaranthus spp.) accessions from central and southern region of Malawi were characterised at Lilongwe University of Agriculture and Natural Resources using agro-morphological traits. A total of thirteen descriptors, defined by Plant Genetic Resources Institute (IPGRI), were used to characterise the amaranth accessions under study. Field experiments were carried out for two seasons in August to November, 2018 and January to March, 2019. The experiments were laid out in a Randomised Complete Block Design (RCBD), which was replicated four times. The qualitative (plant growth habit, leaf colour, inflorescence colour, stem colour, inflorescence spininess, seed colour) and quantitative traits (plant height, stem girth, leaf length, leaf width, inflorescence length, days to 80% flowering, grain yield, leaf yield, and days to 80% maturity) evaluated were significant in defining the uniqueness of different amaranth accessions evaluated. Significant differences (P < 0.05) obtained from analysis of variance were observed in all the parameters studied. Correlation analysis was conducted using Genstat statistical package version 18 while cluster analysis was done using R statistical software. The agro-morphological characterisation results showed a wide range of variation for most of the qualitative characters. Wide variability was present in all the qualitative characters except for plant growth habit where all the accessions exhibited erect plant growth habit. These results point to high possibility of genetic diversity of amaranth accessions in Malawi, it could be exploited in future breeding purposes and deserving conservation.

Genetic Diversity Analysis of Forty Pearl Millet (<i>Pennisetum glaucum</i>(L.) R. Br) Accessions from Sudan Using Agronomical Descriptors and DNA Molecular Markers

Morphological descriptors and Random Amplification of Polymorphic DNA (RAPD) technique were used to assess the genetic variation among and within forty Pennesitum glaucum accessions from Sudan. Accessions were collected from 30 villages representing Darfur, North Kordofan, South Kordofan, and Blue Nile states. 64 amplified fragments were distinguished using ten primers.? 63 bands were polymorphic among the forty accessions with an average of 6.3 polymorphic bands per primer. Low level of genetic similarity was observed (4% - 43%). The PhiPT (analogue of FST fixation index) value for genetic variability obtained over the four regions was 0.169 with high significance (P = 0.01). AMOVA analysis showed higher variance components within regions (80%) than among regions (20%). The two dendrograms obtained by Random Amplification of Polymorphic DNA (RAPD) data;and morphological data based on 26 descriptors did not fit together. PCA (Principal coordinate’s analysis) showed geographic structuring of pearl millet according to its growing regions in Sudan.

Analyses Using SSR and DArT Molecular Markers Reveal that Ethiopian Accessions of White Lupin (<i>Lupinus albus</i>L.) Represent a Unique Genepool

PCR-based genic and microarray-based Diversity Arrays Technology (DArTTM) markers were used to determine genetic diversity in 94 accessions of white lupin (Lupinus albus L.) comprising Australian and foreign cultivars, landraces, and advanced breeding lines from Australian breeding programs. A total of 345 (50 PCR-based and 295 DArT-based) polymorphic fragments were identified, which were used to determine the genetic diversity among accessions. Both cluster analysis of bivariate marker data using UPGMA, and principal coordinate analysis, indicated a high level of genetic diversity in the germplasm. Our results showed that both types of markers used in this study are suitable for estimation of genetic diversity. Landrace accessions from Ethiopia formed a very distinct and separate grouping with both marker systems. Australian cultivars and breeding lines were clustered together and tended to be distinct from European landraces. These findings will allow breeders to select appropriate, diverse parents to broaden the genetic base of white lupin breeding populations.

Genetic Diversity in the Semi-Arid Grown Cowpea(<i>Vigna unguiculata</i>L. Walp)Accessions Using Morphological and Molecular Characterization

Assessment of genetic diversity of the indigenous crop accessions is extremely important for breeders to identify potential parents in cross-breeding programs. Fourteen cowpea accessions collected from different parts of Sudan were used for characterization at morphological and molecular levels. The seeds of the accessions were sown in the field using a randomized complete block design with three replicates. Sixteen morphological descriptors (9 qualitative and 7 quantitative) and 20 Random Amplified Polymorphic DNA (RAPD) markers were used for characterization of the accessions. The results of morphological data revealed considerable variability within and between state’s accessions. Some morphological traits revealed similarity between accessions from different states. Among the 20 RAPD markers used, 18 were polymorphic. A total of 379 polymorphic patterns were generated;polymorphic information content (PIC) ranged from 0.63 to 0.98 with an average of 0.9. The number of fragment detected ranged from 2 for OPL-11 to 51 for OPY-2 with an average of 26.06/primer and 27.07/genotype. One to five (1 - 5) unique fragments of different sizes were detected for particular accessions, which may provide a valuable resource for breeding superior cowpea cultivars in Sudan and other semi-arid zones. Genetic similarity was ranged from 0.02 to 0.47 with an average of 0.25. Highest genetic similarity was between genotypes HSD-2966 and HSD-2967 and between genotypes HSD-5131 and HSD-5627 and the lowest was between HSD-5131 and HSD-5861 followed by that between HSD-2976 and HSD-29130 accessions. The study recommends the combination of morphological and molecular data for more efficient genetic diversity assessment and management.

Comparison of Proximate Composition and Nutritional Qualities of Fifty-Three Cashew Accessions from Burkina Faso

Cashew cultivation is growing in Burkina Faso, but nut production remains low. Identification of high-performance plant material with known varietal characteristics is essential for breeding. This study consisted of physical-chemical and nutritional characterization of almonds from a core selection of 53 cashew accessions from Burkina Faso. Proximate composition included contents in water, lipids, carbohydrates, proteins, ash, cellulose using standard methods. Major constituents were lipids with an average level of 50.71% ± 4.07%, followed by carbohydrates and proteins with average levels of 21.18% ± 3.81% and 20.62% ± 1.58%, respectively. Average water, ash and cellulose levels were 4.56% ± 0.39%, 2.87% ± 0.27% and 4.61% ± 2.52%, respectively. The analysis of variability within accessions, based on physical and chemical parameters, identified 3 groups that differ in lipids, carbohydrates, proteins, cellulose and energy value. The first group containing 17 accessions of fat-rich cashews, average protein levels and very high energy values is more interesting for tree improvement programs.

Biometric Analysis on Genetic Divergence between Parental and Regenerated Accessions in Tall Coconut Palms (Cocos nucifera L.) from International Genebank for Africa and the Indian Ocean

This study endeavour assesses agromorphological likeness between initial introductions and regenerated accessions at the International Coconut Genebank for Africa and the Indian Ocean (ICG-AIO) based in C?te d’Ivoire. Ten couples of parental (G0) and regenerated (G1) accessions of Tall coconut palms were analyzed using Principal Component Analysis (PCA) and Multiple Analysis of Variance (MANOVA) from 26 agromorphological characters. The main results showed a relative decrease in the expression of the phenotypical traits concerning the component of the fruit, height and vigor of the stem and yield of bunches and fruits after one regeneration cycle. But, a high proportion (69%) of studied characters from leaf, inflorescence and nut components showed likeness between G0 and G1 accessions. After one regeneration cycle, the controlled pollination method guarantees significant conservation of the expression of the majority of agromorphological traits. Consequently, regenerated accessions of Tall coconut palms can be used to pursue research and development programs in C?te d’Ivoire.