The calibration accuracy of High Resolution Infrared Radiation Sounder Mod. 2 (HIRS / 2) on NOAA-10 satellite is analyzed in this paper. The non-linear effect in the linear calibration curve induces a deviation of 1.5...The calibration accuracy of High Resolution Infrared Radiation Sounder Mod. 2 (HIRS / 2) on NOAA-10 satellite is analyzed in this paper. The non-linear effect in the linear calibration curve induces a deviation of 1.5 degrees (k) of brightness temperature in the tenth channel (8.3 um, water vapor absorption) of the HIRS/2 and the non-linear effect affects the other channels to a different extent. Based on analyzing non- linearity in two-point calibration curve, a tri-point calibration equation is given. A numerical test of effects of the linear and non-linear calibration models on the accuracy of atmospheric temperature retrievals is carried out.展开更多
Coptis chinensis(Huanglian) is a commonly used traditional Chinese medicine(TCM) herb and alkaloids are the most important chemical constituents in it. In the present study, an isocratic reverse phase high performance...Coptis chinensis(Huanglian) is a commonly used traditional Chinese medicine(TCM) herb and alkaloids are the most important chemical constituents in it. In the present study, an isocratic reverse phase high performance liquid chromatography(RP-HPLC) method allowing the separation of six alkaloids in Huanglian was for the first time developed under the quality by design(Qb D) principles. First, five chromatographic parameters were identified to construct a Plackett-Burman experimental design. The critical resolution, analysis time, and peak width were responses modeled by multivariate linear regression. The results showed that the percentage of acetonitrile, concentration of sodium dodecyl sulfate, and concentration of potassium phosphate monobasic were statistically significant parameters(P < 0.05). Then, the Box-Behnken experimental design was applied to further evaluate the interactions between the three parameters on selected responses. Full quadratic models were built and used to establish the analytical design space. Moreover, the reliability of design space was estimated by the Bayesian posterior predictive distribution. The optimal separation was predicted at 40% acetonitrile, 1.7 g·m L-1of sodium dodecyl sulfate and 0.03 mol·m L-1 of potassium phosphate monobasic. Finally, the accuracy profile methodology was used to validate the established HPLC method. The results demonstrated that the Qb D concept could be efficiently used to develop a robust RP-HPLC analytical method for Huanglian.展开更多
文摘The calibration accuracy of High Resolution Infrared Radiation Sounder Mod. 2 (HIRS / 2) on NOAA-10 satellite is analyzed in this paper. The non-linear effect in the linear calibration curve induces a deviation of 1.5 degrees (k) of brightness temperature in the tenth channel (8.3 um, water vapor absorption) of the HIRS/2 and the non-linear effect affects the other channels to a different extent. Based on analyzing non- linearity in two-point calibration curve, a tri-point calibration equation is given. A numerical test of effects of the linear and non-linear calibration models on the accuracy of atmospheric temperature retrievals is carried out.
基金supported by National Natural Science Foundation of China(No.81403112)Beijing Natural Science Foundation(No.7154217)+1 种基金Scientific Research Program of Beijing University of Chinese Medicine(No.2015-JYB-XS104)Special Program for Beijing Key Laboratory of Chinese Medicine Manufacturing Process Control and Quality Evaluation(No.Z151100001615065)
文摘Coptis chinensis(Huanglian) is a commonly used traditional Chinese medicine(TCM) herb and alkaloids are the most important chemical constituents in it. In the present study, an isocratic reverse phase high performance liquid chromatography(RP-HPLC) method allowing the separation of six alkaloids in Huanglian was for the first time developed under the quality by design(Qb D) principles. First, five chromatographic parameters were identified to construct a Plackett-Burman experimental design. The critical resolution, analysis time, and peak width were responses modeled by multivariate linear regression. The results showed that the percentage of acetonitrile, concentration of sodium dodecyl sulfate, and concentration of potassium phosphate monobasic were statistically significant parameters(P < 0.05). Then, the Box-Behnken experimental design was applied to further evaluate the interactions between the three parameters on selected responses. Full quadratic models were built and used to establish the analytical design space. Moreover, the reliability of design space was estimated by the Bayesian posterior predictive distribution. The optimal separation was predicted at 40% acetonitrile, 1.7 g·m L-1of sodium dodecyl sulfate and 0.03 mol·m L-1 of potassium phosphate monobasic. Finally, the accuracy profile methodology was used to validate the established HPLC method. The results demonstrated that the Qb D concept could be efficiently used to develop a robust RP-HPLC analytical method for Huanglian.
