Enrichment of nervonic acid in Acer truncatum Bunge oil by combination of two-stage molecular distillation,one-stage urea complexation and five-stage solvent crystallization

Nervonic acid is the world’s first and only potent substance that can repair damaged nerve fibers and promote nerve cell regeneration with high nutritional value.The wide variety of fatty acids in plant oils and fats with similar structures makes the large-scale separation and purification of high-purity nervonic acid very difficult.A new combined process of molecular distillation,urea inclusion and solvent crystallization was established to prepare high-purity nervonic acid with the mixed fatty acids obtained after saponification and acidification of Acer truncatum Bunge oil as raw materials.First,according to the difference in the mean free path of fatty acids,molecular distillation was used to separate and remove C16 saturated fatty acid of palmitic acid and four C18-C20 fatty acids of stearic,oleic,linoleic,and linolenic acids.The content of C16-C20 fatty acids decreased from 72.92% to 19.22% after two-stage molecular distillation processes,in which the contents of saturated fatty acid of palmitic acid decreased to about 0.5%.Then,according to the difference in carbon chain length and saturation of fatty acid,the contents of C22-C24 saturated fatty acids of tetracosanoic and docosanoic acids decreased to 0.21% and 0.07% by urea inclusion with urea/free fatty acid preparation by saponification(SPOMFs)ratio as 0.6.In addition,all saturated fatty acids were basically separated.Finally,according to the difference in the solubility of fatty acids in solvents,the C18-C20 unsaturated fatty acids of oleic,linoleic,and linolenic acids and C22 unsaturated fatty acid of erucic acid were removed by solvent crystallization.The content of C18-C20 unsaturated fatty acids decreased to less than 5% with pentanol as the solvent after the first stage solvent crystallization.The content of erucic acid decreased to 3.47% with anhydrous ethanol as the solvent after the second to fifth stage solvent crystallization.The combined process of molecular distillation,urea inclusion and low temperature crystallization innovatively adopted an efficient,simple and easy-toindustrial solvent crystallization method to separate erucic and nervonic acids,obtaining nervonic acid with purity of 96.53% and final yield of 47.99%.

元宝枫种子萌发及其对不同消毒处理的响应

为提高元宝枫(Acer truncatum Bunge)种子萌发率,在DKW无菌培养基中探索不同消毒处理方法对元宝枫种子萌发的影响。结果表明,9种消毒处理下的元宝枫种子的染菌率(真菌和细菌)、保存率及发芽率均存在极显著差异,发芽率与次氯酸钠浓度呈弱正相关,与次氯酸钠处理时间呈强负相关,采用较高浓度的次氯酸钠溶液在较短时间下处理可获得较高发芽率。最佳处理方法为5%的次氯酸钠处理去果翅后的元宝枫带壳种子5min,平均发芽率可达50.19%,最高发芽率可达62.5%。

干旱、半干旱地区元宝枫直播造林技术

依据干旱和半干旱地区气候特点,文章从品种选择、播种准备、播种造林、抚育管理等4个方面对元宝枫(Acer truncatum Bunge.)直播造林技术进行了系统的阐述。旨在发挥元宝枫树种荒山造林的生态价值,促进生态建设和生态修复,助力退耕还林工程实施。