Achillea millefolium and Achillea ptarmica are both plants belonging to the Asteracea family and are traditionally used for their medicinal properties. It has already been shown that some N-alkylamides(NAAs)are respon...Achillea millefolium and Achillea ptarmica are both plants belonging to the Asteracea family and are traditionally used for their medicinal properties. It has already been shown that some N-alkylamides(NAAs)are responsible for these pharmacological actions. Therefore, in the present study, the NAA content of the two plants was analytically characterised. Different extracts were prepared from the roots, the leaves, the stems and the flowers. The structures of NAAs have been assigned in ethanolic extracts of Achillea millefolium and Achillea ptarmica using high performance liquid chromatography – electrospray ionisation – mass spectrometry(HPLC–ESI–MS) and gas chromatography – electron impact – mass spectrometry(GC–EI–MS). Using both analytical techniques, the structures of 14 and 15 NAAs have been assigned in Achillea ptarmica and Achillea millefolium, respectively. Structures of two new NAAs, previously never observed in Achillea ptarmica,were assigned: deca-2E,6Z,8E-trienoic acid 2-methylbutylamide(homospilanthol) or a related isomeric compound and deca-2E,4E-dienoic acid N-methyl isobutylamide. The structure of homospilanthol or a related isomeric compound was also assigned in Achillea millefolium for the first time.展开更多
Traditionally medicinal plants are used for its potential chemotherapeutic action and for safety upon the continued use of these plants.Achillea millefolium(AM)is an ancient herb which is used to treat wounds,headache...Traditionally medicinal plants are used for its potential chemotherapeutic action and for safety upon the continued use of these plants.Achillea millefolium(AM)is an ancient herb which is used to treat wounds,headaches,disorder in gastrointestinal and hepatobiliary,pain,and inflammation.This study was aimed to investigate the biochemical parameter of antimutagenic methanolic extract for AM in vivo.Plant extract together with MTX decreased the damage caused by the drug on the concentrations of total cholesterol and triglycerides(160.3±2.26 and 108.7±2.71),significant increase in total protein concentration(8.51±0.17,9.46±0.19 and 10.8±2.71 g\dL)for 100 and 200 mg\kg was observed.Also plant extracts counteract the damage caused by drug through the reduction for both creatinin and albumin concentrations to 1.57±0.03 mg\dL and 4.56±0.09 g\dL,respectively.On enzymatic level,the results of interactions indicated the ability of plant to modulating harmful effects of the drug(57.1±1.3,130±3.1 and 111.1±2.1 U\L)for GOT,GPT and ALP,respectively.On the other hand,the plant possesses the ability to enhance the concentrations of all immunoglobulin(IgM,IgA and IgG)in the two doses tested.Therefore,the results of this study indicate the antimutagenic potential of AM extract and encouraging its consumption with MTX as one of the promising therapies.展开更多
Cyclophosphamide(CP) is extensively used for the treatment of various cancers,as well as an immunosuppressive agent.However,CP is known to cause several adverse effects including reproductive toxicity.Achillea millefo...Cyclophosphamide(CP) is extensively used for the treatment of various cancers,as well as an immunosuppressive agent.However,CP is known to cause several adverse effects including reproductive toxicity.Achillea millefolium,a widely distributed medicinal plant,is highly regarded for its medicinal activities,including antioxidant and anti-inflammatory properties.The present study was conducted to assess whether Achillea millefolium inflorescences aqueous extract with antioxidant and anti-inflammatory activities could serve as a protective agent against reproductive toxicity during CP treatment.Male Wistar rats were categorized into four groups.Two groups of rats were administered CP at a dose of 5 mg·kg-1·d-1 for 28 d by oral gavages.One of these groups received Achillea aqueous extract at a dose of 1.2 g·kg-1·d-1 orally 4 h after cyclophosphamide administration.A vehicle treated control group and an Achillea control group were also included.The CP-treated group showed significant decreases in the body,testes and epidi-dymides weights as well as many histological alterations.Stereological parameters,spermatogenic activities and testicular antioxidant capacity along with epididymal sperm count and serum testosterone concentration were also significantly decreased by CP treatment.Notably,Achillea co-administration caused a partial recovery in above-mentioned parameters.These findings indicate that Achillea millefolium inflorescence aqueous extract may be partially protective against CP-induced testicular toxicity.展开更多
基金the Special Research Fund of Ghent University (BOF 01D23812 to Lien Taevernier)
文摘Achillea millefolium and Achillea ptarmica are both plants belonging to the Asteracea family and are traditionally used for their medicinal properties. It has already been shown that some N-alkylamides(NAAs)are responsible for these pharmacological actions. Therefore, in the present study, the NAA content of the two plants was analytically characterised. Different extracts were prepared from the roots, the leaves, the stems and the flowers. The structures of NAAs have been assigned in ethanolic extracts of Achillea millefolium and Achillea ptarmica using high performance liquid chromatography – electrospray ionisation – mass spectrometry(HPLC–ESI–MS) and gas chromatography – electron impact – mass spectrometry(GC–EI–MS). Using both analytical techniques, the structures of 14 and 15 NAAs have been assigned in Achillea ptarmica and Achillea millefolium, respectively. Structures of two new NAAs, previously never observed in Achillea ptarmica,were assigned: deca-2E,6Z,8E-trienoic acid 2-methylbutylamide(homospilanthol) or a related isomeric compound and deca-2E,4E-dienoic acid N-methyl isobutylamide. The structure of homospilanthol or a related isomeric compound was also assigned in Achillea millefolium for the first time.
文摘Traditionally medicinal plants are used for its potential chemotherapeutic action and for safety upon the continued use of these plants.Achillea millefolium(AM)is an ancient herb which is used to treat wounds,headaches,disorder in gastrointestinal and hepatobiliary,pain,and inflammation.This study was aimed to investigate the biochemical parameter of antimutagenic methanolic extract for AM in vivo.Plant extract together with MTX decreased the damage caused by the drug on the concentrations of total cholesterol and triglycerides(160.3±2.26 and 108.7±2.71),significant increase in total protein concentration(8.51±0.17,9.46±0.19 and 10.8±2.71 g\dL)for 100 and 200 mg\kg was observed.Also plant extracts counteract the damage caused by drug through the reduction for both creatinin and albumin concentrations to 1.57±0.03 mg\dL and 4.56±0.09 g\dL,respectively.On enzymatic level,the results of interactions indicated the ability of plant to modulating harmful effects of the drug(57.1±1.3,130±3.1 and 111.1±2.1 U\L)for GOT,GPT and ALP,respectively.On the other hand,the plant possesses the ability to enhance the concentrations of all immunoglobulin(IgM,IgA and IgG)in the two doses tested.Therefore,the results of this study indicate the antimutagenic potential of AM extract and encouraging its consumption with MTX as one of the promising therapies.
基金supported by Urmia University,Faculty of Veterinary Medicine as a part of Ph. D thesis in histology
文摘Cyclophosphamide(CP) is extensively used for the treatment of various cancers,as well as an immunosuppressive agent.However,CP is known to cause several adverse effects including reproductive toxicity.Achillea millefolium,a widely distributed medicinal plant,is highly regarded for its medicinal activities,including antioxidant and anti-inflammatory properties.The present study was conducted to assess whether Achillea millefolium inflorescences aqueous extract with antioxidant and anti-inflammatory activities could serve as a protective agent against reproductive toxicity during CP treatment.Male Wistar rats were categorized into four groups.Two groups of rats were administered CP at a dose of 5 mg·kg-1·d-1 for 28 d by oral gavages.One of these groups received Achillea aqueous extract at a dose of 1.2 g·kg-1·d-1 orally 4 h after cyclophosphamide administration.A vehicle treated control group and an Achillea control group were also included.The CP-treated group showed significant decreases in the body,testes and epidi-dymides weights as well as many histological alterations.Stereological parameters,spermatogenic activities and testicular antioxidant capacity along with epididymal sperm count and serum testosterone concentration were also significantly decreased by CP treatment.Notably,Achillea co-administration caused a partial recovery in above-mentioned parameters.These findings indicate that Achillea millefolium inflorescence aqueous extract may be partially protective against CP-induced testicular toxicity.
