Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing

method of extracting astaxanthin from Phaffia rhodozyma with various solvents after acid washing was investigated. The extraction efficiency was distinctly increased after acid washing of P. rhodozyma cells. When the concentration of HCl was 0.4 mol.L^-1, the highest extraction efficiency of astaxanthin was achieved which was about three times higher than the control. Acetone or benzene as single polar or non-polar solvent was the most ef- fective solvent in our research. With a combination of isopropanol and n-hexane （volume ratio of 2 ： 1）, the maxi- mal extraction efficiency was achieved, approximately 60% higher than that obtained with a single solvent. The liquid-solid ratio and the extracting time were also optimized. Under the optimum extraction conditions, the extraction yield of astaxanthin exceeded 98%.

Impact of Stone Enzyme Wash and Acid Wash Based on Denim Garments

The aim of this project is to find out the changes that occur in physical properties of denim when it is subjected to enzyme stone and acid wash or to find out the impact of enzyme stone and acid wash. 97% cotton 3% elastomer twill, weave 3/1, construction 72 × 40/9 × 7 indigo dyed denim fabric leg panels as per lab standard recipe are used here to examine. Firstly, desizing was done as pre-treatments and after treatment was silicon softener. After washing process, different samples from both washing are going to express different behavior on physical properties. This experiment is done to find out the discrimination in tearing strength, shrinkage %, color fastness to wash, color fastness to rubbing, pH rate between stone enzyme wash and acid wash of denim garments.

Investigation on Effect of Acid Wash with Thermocol Ball on Physical Properties of Knitted Garments

Washing is considered as the final process of garments finishing. Acid washing is one kind of finishing treatment used for making sewn garments aesthetic, fashionable, soft, comfortable and also adding value to the final garments. This paper investigates the effect of the acid washing (with thermocol balls and potassium permanganate) on different physical properties of three knitted garments (Single Jersey 100% Cotton T-shirt, Single Jersey 95% Cotton 5% Spandex T-Shirt and 1 × 1 Rib100% Cotton T-Shirt). Typical washing procedures and techniques were followed and then physical properties were analyzed under standard condition. It is observed that fabric weight, CPI, WPI, spirality and shrinkage increase while bursting strength, stitch length absorbency decrease after washing treatment. pH of all the samples is under controlled and lies between 7 to 8. There is no change in pilling, colorfastness to wash, water and dry rubbing while a little bit decrease in wet rubbing.

A comparative study on demineralization of coals by acid-washing and solvent-extraction methods for low temperature catalytic coal gasification application

Catalytic coal gasification is an efficient way to achieve high conversion rates at low temperatures.Catalyst loss due to the reaction between the inherent mineral matter in the coal and the catalyst is a major hurdle for its practical application.The problem may be overcome by removing the mineral matter from the coal before use.Two demineralizing approaches,acid-washing and solvent-extraction methods are investigated for demineralizing the coals.Elemental analysis,XRD analysis,NMR analysis,and N_(2)adsorption analysis were made to compare the demineralization efficiency,crystallographic carbon microstructure,chemical structure,and micro-porosity,respectively.Solvent-extraction method was found be more efficient for demineralization.Difference was observed in the chemical structure and micro-porosity while crystallographic carbon-microstructure was found to be similar.Non-catalytic and catalytic gasification reactivity of the chars obtained by the two methods was compared.Gas composition and gas evolution profiles were also obtained and found to be influenced by the degree of demineralization.

Experimental study of the influence of acid wash on cellulose pyrolysis

The analysis of microstructure and polymeriza-tion degree showed that acid wash altered the cellulose morphology and decreased the polymerization degree signifi-cantly.A series of experiments were done to study the effect of acid wash on cellulose rapid pyrolysis.Experimental results showed that under acid pretreatment,the yield of bio-oil decreased while the production of gas and char increased.With an increase in acid concentration,this trend would be further enhanced.Sulphuric acid limited the formation of bio-oil more effectively than hydrochloric acid and pho-sphoric acid.According to the GC-MS analysis of bio-oil,high-concentration acid wash restrained the formation of levoglucosan by catalyzing dehydration process and cross linking reaction.

Tannic acid and saponin for removing arsenic from brownfield soils: Mobilization, distribution and speciation

Plant biosurfactants were used for the first time to remove As and co-existing metals from brownfield soils. Tannic acid （TA）, a polyphenol, and saponin （SAP）, a glycoside were tested. The soil washing experiments were performed in batch conditions at constant biosurfactant concentration （3%）. Both biosurfactants differed in natural pH, surface tension, critical micelle concentration and content of functional groups. After a single washing, TA （pH 3.44） more efficiently mobilized As than SAP （pH 5.44）. When both biosurfactants were used at the same pH （SAP adjusted to 3.44）, arsenic mobilization was improved by triple washing. The process efficiency for TA and SAP was similar, and depending on the soil sample, ranged between 50%-64%. Arsenic mobilization by TA and SAP resulted mainly from decomposition of Fe arsenates, followed by Fe3＋ complexation with biosurfactants. Arsenic was efficiently released from reducible and partially from residual fractions. In all soils, As（V） was almost completely removed, whereas content of As（III） was decreased by 37%-73%. SAP and TA might be used potentially to remove As from contaminated soils.

Stresses Imposed on Yeast During Brewing Fermentations and Their Effect on Cellular Activity

Brewer’s yeast strains are exposed to a spectrum of threats and“in-sults”thatcultures encounter during wort fermentation.Culture storage(including acid washing)andtheir propagation can also stress yeast systems.Stresses are a plethora of parameters that ayeast culture toler-ates during its propagation,stprage,and throughout and between wortfermentations.Stress can result in a number of effects on a yeast cul-ture.These includedecreases in yeast viability and vitality,depletion of intracellular glycogen,increases intrehalose,and excretion of intra-cellular proteinase A and other proteinases,with effects onbeer foam stability.Shear of cell wall components will also occur.In addition,modification ofa culture’s flocculation characteristics can result,to-gether with the formation of unfilterablemannan-protein beer hazes,Finally,increases in respiratory deficient(petite)and other yeastmu-tants have been reported.

An Improved Method of Laser Particle Size Analysis and Its Applications in Identification of Lacustrine Tempestite and Beach Bar：An Example from the Dongying Depression

Grain size analysis is a common method in the study of sedimentology. For the consolidated sedimentary rocks, the traditional methods are rock slice observation and image analysis. In recent years, laser particle size analyzer is used widely in particle size analysis of sedimentary rock. Unlike the pretreatment of loose samples, the rock samples must be crushed, added acid to wipe out cement, and washed. However, in the step of washing, most of the fines component（less than 63 μm） in the suspended state should be inevitably lost. It will significantly affect the accuracy of particle size analysis, especially for siltstone. This paper presents a siltstone sample pretreatment method which core step is washing acid by centrifuge. Compared with traditional decantation method, the results show that the median particle size reduced 33.2 μm on average. Compared with the precipitation method which is commonly used for handling loose samples, the change of solid-liquid separation time is from 12 h to 10 min, while the average reduction of median particle size is about 15 μm. The grain size value corresponded to the cumulative volume of 10%/90% reduced 2.5 μm/20.3 μm on average. The percentage of the clay component less than 2 μm increased 2.88% on average. The fine particle（2–4 μm） and silt component（4–63 μm） increased 1.71% and 5.56% on average. Based on this method, two kinds of similar lacustrine siltstone were analyzed. They are tempestite and beach bar which are difficult to identify in the Lijin sub-depression, Dongying depression, Shengli oilfield, China. The final grain-size probability plot of tempestite is the type of “one saltation component and three suspension components”. The content of suspension components can reach to 80%–90%. The beach bar is the type of “one saltation component and two suspension components”. The content of suspension components can reach to 40%–45%. They both have the characteristics of high slope which means well sorting. But they can be distinguished based on the suspension sedimentary characteristics which were preserved by maximum degree in this kind of sample pretreatment method.