目的:观察乳酸杆菌对蒙古沙土鼠H pylori感染性胃炎的治疗作用,探讨乳酸杆菌防治H pylori感染的作用机制.方法:通过接种H pylori,建立蒙古沙土鼠H p y l o r i感染性胃炎动物模型,将造模成功的32只蒙古沙土鼠随机分为4组,每组8只.第1组...目的:观察乳酸杆菌对蒙古沙土鼠H pylori感染性胃炎的治疗作用,探讨乳酸杆菌防治H pylori感染的作用机制.方法:通过接种H pylori,建立蒙古沙土鼠H p y l o r i感染性胃炎动物模型,将造模成功的32只蒙古沙土鼠随机分为4组,每组8只.第1组(抗生素加质子泵抑制剂干预治疗组)、第2组(乳酸杆菌治疗组)、第3组(乳酸杆菌加质子泵抑制剂治疗组)、第4组(生理盐水对照组),用相应干扰剂分别连续灌胃治疗10d.另8只正常蒙古沙土鼠为第5组(空白对照组).最后1次灌胃4wk后处死沙鼠,HE染色判断鼠胃黏膜组织学损伤,并用快速尿素酶试验、Giemsa染色和细菌培养进行H pylori的检测,免疫组化检测胃黏膜中IL-6、COX-2、和TNF-α蛋白的表达.结果:第1组、第2组和第3组之间蒙古沙土鼠的H pylori根除率无显著差异,但均比第4组的根除率低,之间差异有显著性(87.5%,75%,75%vs100%,均P<0.05).第1组、第2组和第3组蒙古沙土鼠的胃体、胃窦和十二指肠各部位组织胃黏膜慢性炎症评分与第4组相比显著下降(胃体:1.8±0.54,2.0±0.48,1.9±0.50vs4.2±1.06,均P<0.05;胃窦:1.5±0.27,1.4±0.33,1.7±0.46vs4.6±0.74,均P<0.05;十二指肠:1.4±0.41,1.7±0.29,1.6±0.52vs4.1±0.83,均P<0.05).第1组、第2组和第3组蒙古沙土鼠三种炎症因子表达与第4组相比显著下降(COX-2:3.57±0.32,3.89±0.51,2.95±0.76vs6.79±1.68,均P<0.05;IL-6:2.42±0.60,2.28±0.71,1.93±0.52vs5.61±0.28,均P<0.05;TNF-α:3.30±0.52,3.65±0.35,2.84±0.20vs6.76±1.63,均P<0.05).结论:乳酸杆菌治疗蒙古沙鼠H pylori感染性胃炎模型的疗效与PPI加抗生素三联疗法相当;并可显著降低沙鼠胃黏膜的COX-2、IL-6、TNF-α的表达.展开更多
Aims: To observe the clinical effect of acupuncture plus acupoint injection of Acidi Nucleici et Caseini Hydrolysatis Composita in the treatment of allergic rhinitis. Methods: Ninety five cases of allergic rhinitis pa...Aims: To observe the clinical effect of acupuncture plus acupoint injection of Acidi Nucleici et Caseini Hydrolysatis Composita in the treatment of allergic rhinitis. Methods: Ninety five cases of allergic rhinitis patients were randomized into acupuncture plus acupoint injection group (treatment group, n=40), acupuncture group (control group A, n=30) and muscular injection group (control group B, n=25). Yingxiang (LI 20), Yintang (EX HN 3), Baihui (GV 20), Fengchi (GB 20), Fengmen (BL 12), Feishu (BL 13), etc. were used. Results: Following 20 days’ treatment, of the 40, 30 and 25 cases of allergic rhinitis in treatment, control A and B groups, 16 (40.0%), 8 (26.7%) and 0 were cured, 22 (55.0%), 14 (46.7%) and 4 (16.0%) experienced improvement in their symptoms and signs, and 2 (5.0%), 8 (26.7%) and 21 (84.0%) failed in the treatment, with the effective rates being 95.0%, 73.3% and 16.0% separately. The therapeutic effect of treatment group was significantly superior to those of two control groups (P<0.05, 0.01). Conclusion: The therapeutic effect of acupuncture plus acupoint injection of Injecto Acidi Nucleici et Caseini Hydrolysatis Composita is definite.展开更多
文摘目的:观察乳酸杆菌对蒙古沙土鼠H pylori感染性胃炎的治疗作用,探讨乳酸杆菌防治H pylori感染的作用机制.方法:通过接种H pylori,建立蒙古沙土鼠H p y l o r i感染性胃炎动物模型,将造模成功的32只蒙古沙土鼠随机分为4组,每组8只.第1组(抗生素加质子泵抑制剂干预治疗组)、第2组(乳酸杆菌治疗组)、第3组(乳酸杆菌加质子泵抑制剂治疗组)、第4组(生理盐水对照组),用相应干扰剂分别连续灌胃治疗10d.另8只正常蒙古沙土鼠为第5组(空白对照组).最后1次灌胃4wk后处死沙鼠,HE染色判断鼠胃黏膜组织学损伤,并用快速尿素酶试验、Giemsa染色和细菌培养进行H pylori的检测,免疫组化检测胃黏膜中IL-6、COX-2、和TNF-α蛋白的表达.结果:第1组、第2组和第3组之间蒙古沙土鼠的H pylori根除率无显著差异,但均比第4组的根除率低,之间差异有显著性(87.5%,75%,75%vs100%,均P<0.05).第1组、第2组和第3组蒙古沙土鼠的胃体、胃窦和十二指肠各部位组织胃黏膜慢性炎症评分与第4组相比显著下降(胃体:1.8±0.54,2.0±0.48,1.9±0.50vs4.2±1.06,均P<0.05;胃窦:1.5±0.27,1.4±0.33,1.7±0.46vs4.6±0.74,均P<0.05;十二指肠:1.4±0.41,1.7±0.29,1.6±0.52vs4.1±0.83,均P<0.05).第1组、第2组和第3组蒙古沙土鼠三种炎症因子表达与第4组相比显著下降(COX-2:3.57±0.32,3.89±0.51,2.95±0.76vs6.79±1.68,均P<0.05;IL-6:2.42±0.60,2.28±0.71,1.93±0.52vs5.61±0.28,均P<0.05;TNF-α:3.30±0.52,3.65±0.35,2.84±0.20vs6.76±1.63,均P<0.05).结论:乳酸杆菌治疗蒙古沙鼠H pylori感染性胃炎模型的疗效与PPI加抗生素三联疗法相当;并可显著降低沙鼠胃黏膜的COX-2、IL-6、TNF-α的表达.
文摘Aims: To observe the clinical effect of acupuncture plus acupoint injection of Acidi Nucleici et Caseini Hydrolysatis Composita in the treatment of allergic rhinitis. Methods: Ninety five cases of allergic rhinitis patients were randomized into acupuncture plus acupoint injection group (treatment group, n=40), acupuncture group (control group A, n=30) and muscular injection group (control group B, n=25). Yingxiang (LI 20), Yintang (EX HN 3), Baihui (GV 20), Fengchi (GB 20), Fengmen (BL 12), Feishu (BL 13), etc. were used. Results: Following 20 days’ treatment, of the 40, 30 and 25 cases of allergic rhinitis in treatment, control A and B groups, 16 (40.0%), 8 (26.7%) and 0 were cured, 22 (55.0%), 14 (46.7%) and 4 (16.0%) experienced improvement in their symptoms and signs, and 2 (5.0%), 8 (26.7%) and 21 (84.0%) failed in the treatment, with the effective rates being 95.0%, 73.3% and 16.0% separately. The therapeutic effect of treatment group was significantly superior to those of two control groups (P<0.05, 0.01). Conclusion: The therapeutic effect of acupuncture plus acupoint injection of Injecto Acidi Nucleici et Caseini Hydrolysatis Composita is definite.
