The establishment of biosafety system is of enormous importance to the livestock and poultry production in terms of mitigating the transmission of diseases and implementing regional prevention and control measures.How...The establishment of biosafety system is of enormous importance to the livestock and poultry production in terms of mitigating the transmission of diseases and implementing regional prevention and control measures.However,the current sterilization technology presents several drawbacks,including time-consuming procedures,chemical residues,and challenges in treating the sewage after rinsing.In this study,a novel cleaning and sterilization method that combines slightly acidic electrolyzed water and high pressure water-jet was developed.An orthogonal test was conducted to examine the correlation between high-pressure conditions and the various non-structural parameters on the efficacy of sterilization rate.In a field test,the effectiveness of the technology in cleaning pig transfer vehicles was evaluated by the total plate count and variations of community composition.The findings revealed that the combination of process parameters,including an available chlorine concentration of 200 mg/L,rinsing pressure of 170 bar,rinsing duration of 10 s,and residence time of 15 min,resulted in a removal rate of colony concentration on the surface of pig transfer vehicles of(96.50±0.91)%.Moreover,it was demonstrated to effectively inhibit a variety of pathogenic bacteria.The innovative cleaning system has the potential to replace traditional methods and reduces pollution while saving time and labor.It introduces a novel approach for sterilization of transportation in livestock and poultry farms as well as the biosafety construction of the animal husbandry.展开更多
In the present study,the bactericidal efficacy of slightly acidic electrolyzed water(SAEW)against Listeria monocytogenes(L.monocytogenes)planktonic cells and biofilm on food-contact surfaces including stainless steel ...In the present study,the bactericidal efficacy of slightly acidic electrolyzed water(SAEW)against Listeria monocytogenes(L.monocytogenes)planktonic cells and biofilm on food-contact surfaces including stainless steel and glass was systematically evaluated.The results showed that SAEW(pH 5.09 and available chlorine concentration(ACC)of 60.33 mg/L)could kill L.monocytogenes on food-contact surfaces completely in 30 s,a disinfection efficacy equal to that of NaCIO solutions(pH 9.23 and ACC of 253.53 mg/L).The results showed that long exposure time and high ACC contributed to the enhancement of the disinfection efficacy of SAEW on L.monocytogenes on food-contact surfaces.Moreover,the log reduction of SAEW treatment presented an increasing tendency within the prolonging of treatment time when SAEW was used to remove the L.monocytogenes bioflm formed on stainless steel and glass surfaces,which suggested that SAEW could remove L.monocytogenes bio-film effectively and its disinfection efficacy is equal to(in the case of stainless steel)or higher than(in the case of glass)that of high-ACC NaCIO solutions.In addition,the results of the crystal violet staining and scanning electron microscopy also demonstrated that SAEW treatment could remove the L.monocytogenes biofilm on food-contact surfaces.展开更多
Slightly acidic electrolyzed water(SAEW,pH 6.0-6.5)is an ideal and environmentally-friendly disinfectant,which was used to prevent and control bacterial infections on farms.This work aims to investigate the inactivati...Slightly acidic electrolyzed water(SAEW,pH 6.0-6.5)is an ideal and environmentally-friendly disinfectant,which was used to prevent and control bacterial infections on farms.This work aims to investigate the inactivation effectiveness of SAEW in inactivating microbes in a disinfection channel.The bactericidal efficiency of SAEW on equipment surfaces was compared to two commercial disinfectants,Kuei A bromide solution(KAS,5:1000 v/v)and Glutaraldehyde solution(GS,5:1000 v/v).The disinfection effectiveness of SAEW in inactivating Salmonella enteritidis(S.enteritidis)on equipment surfaces in the disinfection channel was evaluated,and a model was developed using multiple linear regression analysis.Results indicated that SAEW was significantly(p<0.05)more efficient than KAS and GS on kits and clothing in the disinfection channel at 1 min.The SAEW did not contribute as aggressively to respiratory difficulty as KAS and GS.Maximum reductions of 2.362 log10 CFU/cm^(2),2.613 log10 CFU/cm^(2) and 2.359 log10 CFU/cm^(2) for Salmonella enteritidis were obtained from clothing surfaces,iron materials,and kits treated with SAEW for 2.5 min at a chlorine concentration of 220 mg/L.Moreover,the established model had a good fit-quantified by the determination coefficient R^(2)(0.939)and a lack of fit test(p>0.05).In addition,available chlorine concentration(ACC)was an important factor than other factors,and the inactivation efficiency of Salmonella enteritidis sprayed by SAEW treatment was different between iron materials,kits and clothing surfaces(iron>kit>clothing).展开更多
This study assessed the combined effect of slightly acidic electrolyzed water(SAEW)and slightly acidic electrolyzed water ice(SAEW-ice)on the quality of pomfrets over a period of 18 d of cold storage at 4°C.A pre...This study assessed the combined effect of slightly acidic electrolyzed water(SAEW)and slightly acidic electrolyzed water ice(SAEW-ice)on the quality of pomfrets over a period of 18 d of cold storage at 4°C.A presoak for 5 min in SAEW solution(22 mg/L)was used before the pomfrets were placed on SAEW-ice(pH:6.45;ORP:803 mV;ACC:18 mg/L);The changes in physicochemical properties(i.e.,pH,thiobarbituric acid,total volatile basic nitrogen and texture profile),microbial loads and sensory characteristics were all analyzed.Compared with the tap water(TW)group,the total bacterial counts of the SAEW group significantly decreased by 1.27 log10 CFU/g after immersion(p<0.05).The shelf life of the pomfrets was prolonged by 9 d by the combined treatment of SAEW and SAEW-ice during storage at 4℃.On the 18th day,the gumminess and chewiness values of the pomfrets in the SAEW+SAEW-ice group were 195 g and 3.97 mJ,respectively,which were significantly higher than those of the other groups(p<0.05).The results suggested that SAEW+SAEW-ice treatments have great potential as a novel method to maintain the quality and extend the shelf life of pomfrets during refrigerated storage.展开更多
Mixed solution of slightly acidic electrolyzed water(SAEW)and artificial seawater was used to investigate the disinfection potential of SAEW in artificial seawater.Inoculated Vibrio parahaemolyticus(suspended in 3%sod...Mixed solution of slightly acidic electrolyzed water(SAEW)and artificial seawater was used to investigate the disinfection potential of SAEW in artificial seawater.Inoculated Vibrio parahaemolyticus(suspended in 3%sodium chloride alkaline peptone water and 0.85%sodium chloride water,respectively)was subjected to different mixed-SAEW and SAEW immersion treatments(5-20 mg/L available chlorine concentration(ACC)).In the presence of organic matter,4.07 logCFU/mL significant reduction(p<0.05)was achieved after treating with 20 mg/L mixed-SAEW for 15 min.There was 5.13 logCFU/mL reduction after treating with 15 mg/L SAEW for 15 min.For V.parahaemolyticus suspended in 0.85%sodium chloride solution,it was undetected after 30 s SAEW treatment(5 mg/L ACC)or 120 s mixed-SAEW treatment(10 mg/L ACC).At a ratio of SAEW and artificial seawater at 1:15(V/V),SAEW could inactivate V.parahaemolyticus to undetectable level in artificial seawater in one minute,which was comparable with UV treatment of 10 W.The results indicated high sanitization potential of SAEW against V.parahaemolyticus in aquaculture seawater.展开更多
为进一步推广弱酸性电位水(SAEW)在生鲜果蔬表面除菌上的应用,以樱桃番茄和草莓为原料,探究了不同浸泡时间(0、1、3、5、10 min)下SAEW的除菌效果。结果发现,樱桃番茄最适浸泡时间为3min,此时其表面菌落总数、霉菌和酵母、鼠伤寒沙门氏...为进一步推广弱酸性电位水(SAEW)在生鲜果蔬表面除菌上的应用,以樱桃番茄和草莓为原料,探究了不同浸泡时间(0、1、3、5、10 min)下SAEW的除菌效果。结果发现,樱桃番茄最适浸泡时间为3min,此时其表面菌落总数、霉菌和酵母、鼠伤寒沙门氏菌分别减少了1.38、1.29、1.27 lg CFU/g;浸泡时间5min后,草莓表面微生物降低值基本不再提高,菌落总数、霉菌和酵母、鼠伤寒沙门氏菌分别降低1.03、0.91、1.24 lg CFU/g。同时,研究还发现,SAEW处理后样品的呼吸速率会加快,但硬度、可滴定酸、可溶性固形物、维生素C、花青素等各项品质参数基本无变化,SAEW浸泡不会导致样品品质下降。展开更多
基金support of this project by the Strategic Priority Research Program of the National Center of Technology Innovation for Pigs(Grant No.NCTIP-XD/B07).
文摘The establishment of biosafety system is of enormous importance to the livestock and poultry production in terms of mitigating the transmission of diseases and implementing regional prevention and control measures.However,the current sterilization technology presents several drawbacks,including time-consuming procedures,chemical residues,and challenges in treating the sewage after rinsing.In this study,a novel cleaning and sterilization method that combines slightly acidic electrolyzed water and high pressure water-jet was developed.An orthogonal test was conducted to examine the correlation between high-pressure conditions and the various non-structural parameters on the efficacy of sterilization rate.In a field test,the effectiveness of the technology in cleaning pig transfer vehicles was evaluated by the total plate count and variations of community composition.The findings revealed that the combination of process parameters,including an available chlorine concentration of 200 mg/L,rinsing pressure of 170 bar,rinsing duration of 10 s,and residence time of 15 min,resulted in a removal rate of colony concentration on the surface of pig transfer vehicles of(96.50±0.91)%.Moreover,it was demonstrated to effectively inhibit a variety of pathogenic bacteria.The innovative cleaning system has the potential to replace traditional methods and reduces pollution while saving time and labor.It introduces a novel approach for sterilization of transportation in livestock and poultry farms as well as the biosafety construction of the animal husbandry.
基金the Hebei Natural Science Foundation of China(No.C2018208085)the National Natural Science Foundation of China(No.31972170).
文摘In the present study,the bactericidal efficacy of slightly acidic electrolyzed water(SAEW)against Listeria monocytogenes(L.monocytogenes)planktonic cells and biofilm on food-contact surfaces including stainless steel and glass was systematically evaluated.The results showed that SAEW(pH 5.09 and available chlorine concentration(ACC)of 60.33 mg/L)could kill L.monocytogenes on food-contact surfaces completely in 30 s,a disinfection efficacy equal to that of NaCIO solutions(pH 9.23 and ACC of 253.53 mg/L).The results showed that long exposure time and high ACC contributed to the enhancement of the disinfection efficacy of SAEW on L.monocytogenes on food-contact surfaces.Moreover,the log reduction of SAEW treatment presented an increasing tendency within the prolonging of treatment time when SAEW was used to remove the L.monocytogenes bioflm formed on stainless steel and glass surfaces,which suggested that SAEW could remove L.monocytogenes bio-film effectively and its disinfection efficacy is equal to(in the case of stainless steel)or higher than(in the case of glass)that of high-ACC NaCIO solutions.In addition,the results of the crystal violet staining and scanning electron microscopy also demonstrated that SAEW treatment could remove the L.monocytogenes biofilm on food-contact surfaces.
基金The author gratefully acknowledges the financial support from China Agricultural Research Systems(CARS-41)Southern plant-eating livestock microclimate regulation technology research(201303145)Science and Technology Plan Projects of Department of Education of Jiangxi Province(Grant No.:GJJ160401).The author also likes to acknowledge Zhao Lijie for assisting with the experiments.
文摘Slightly acidic electrolyzed water(SAEW,pH 6.0-6.5)is an ideal and environmentally-friendly disinfectant,which was used to prevent and control bacterial infections on farms.This work aims to investigate the inactivation effectiveness of SAEW in inactivating microbes in a disinfection channel.The bactericidal efficiency of SAEW on equipment surfaces was compared to two commercial disinfectants,Kuei A bromide solution(KAS,5:1000 v/v)and Glutaraldehyde solution(GS,5:1000 v/v).The disinfection effectiveness of SAEW in inactivating Salmonella enteritidis(S.enteritidis)on equipment surfaces in the disinfection channel was evaluated,and a model was developed using multiple linear regression analysis.Results indicated that SAEW was significantly(p<0.05)more efficient than KAS and GS on kits and clothing in the disinfection channel at 1 min.The SAEW did not contribute as aggressively to respiratory difficulty as KAS and GS.Maximum reductions of 2.362 log10 CFU/cm^(2),2.613 log10 CFU/cm^(2) and 2.359 log10 CFU/cm^(2) for Salmonella enteritidis were obtained from clothing surfaces,iron materials,and kits treated with SAEW for 2.5 min at a chlorine concentration of 220 mg/L.Moreover,the established model had a good fit-quantified by the determination coefficient R^(2)(0.939)and a lack of fit test(p>0.05).In addition,available chlorine concentration(ACC)was an important factor than other factors,and the inactivation efficiency of Salmonella enteritidis sprayed by SAEW treatment was different between iron materials,kits and clothing surfaces(iron>kit>clothing).
基金This study was financially supported by the National Key Research and Development Program of China(Grant No.2018YFD0701001,Grant No.2017YFB0404000).Any opinions,findings,and conclusions expressed in this publication are those of the authors and do not necessarily reflect the views of Zhejiang University.
文摘This study assessed the combined effect of slightly acidic electrolyzed water(SAEW)and slightly acidic electrolyzed water ice(SAEW-ice)on the quality of pomfrets over a period of 18 d of cold storage at 4°C.A presoak for 5 min in SAEW solution(22 mg/L)was used before the pomfrets were placed on SAEW-ice(pH:6.45;ORP:803 mV;ACC:18 mg/L);The changes in physicochemical properties(i.e.,pH,thiobarbituric acid,total volatile basic nitrogen and texture profile),microbial loads and sensory characteristics were all analyzed.Compared with the tap water(TW)group,the total bacterial counts of the SAEW group significantly decreased by 1.27 log10 CFU/g after immersion(p<0.05).The shelf life of the pomfrets was prolonged by 9 d by the combined treatment of SAEW and SAEW-ice during storage at 4℃.On the 18th day,the gumminess and chewiness values of the pomfrets in the SAEW+SAEW-ice group were 195 g and 3.97 mJ,respectively,which were significantly higher than those of the other groups(p<0.05).The results suggested that SAEW+SAEW-ice treatments have great potential as a novel method to maintain the quality and extend the shelf life of pomfrets during refrigerated storage.
基金This research was financially supported by the National Key R&D Program of China(Project number:2018YFD0701001)the Key Laboratory of Equipment and Informatization in Environment Controlled Agriculture,Ministry of Agriculture,P.R.China and the Shanghai Sailing Program(19YF1443500).
文摘Mixed solution of slightly acidic electrolyzed water(SAEW)and artificial seawater was used to investigate the disinfection potential of SAEW in artificial seawater.Inoculated Vibrio parahaemolyticus(suspended in 3%sodium chloride alkaline peptone water and 0.85%sodium chloride water,respectively)was subjected to different mixed-SAEW and SAEW immersion treatments(5-20 mg/L available chlorine concentration(ACC)).In the presence of organic matter,4.07 logCFU/mL significant reduction(p<0.05)was achieved after treating with 20 mg/L mixed-SAEW for 15 min.There was 5.13 logCFU/mL reduction after treating with 15 mg/L SAEW for 15 min.For V.parahaemolyticus suspended in 0.85%sodium chloride solution,it was undetected after 30 s SAEW treatment(5 mg/L ACC)or 120 s mixed-SAEW treatment(10 mg/L ACC).At a ratio of SAEW and artificial seawater at 1:15(V/V),SAEW could inactivate V.parahaemolyticus to undetectable level in artificial seawater in one minute,which was comparable with UV treatment of 10 W.The results indicated high sanitization potential of SAEW against V.parahaemolyticus in aquaculture seawater.
文摘为进一步推广弱酸性电位水(SAEW)在生鲜果蔬表面除菌上的应用,以樱桃番茄和草莓为原料,探究了不同浸泡时间(0、1、3、5、10 min)下SAEW的除菌效果。结果发现,樱桃番茄最适浸泡时间为3min,此时其表面菌落总数、霉菌和酵母、鼠伤寒沙门氏菌分别减少了1.38、1.29、1.27 lg CFU/g;浸泡时间5min后,草莓表面微生物降低值基本不再提高,菌落总数、霉菌和酵母、鼠伤寒沙门氏菌分别降低1.03、0.91、1.24 lg CFU/g。同时,研究还发现,SAEW处理后样品的呼吸速率会加快,但硬度、可滴定酸、可溶性固形物、维生素C、花青素等各项品质参数基本无变化,SAEW浸泡不会导致样品品质下降。
