In this work, the optimization of hydrogen production by photo-fermentation bacteria immobilized on agar gel granule was systematic investigated in batch culture. Experiment focus on the effect of some important affec...In this work, the optimization of hydrogen production by photo-fermentation bacteria immobilized on agar gel granule was systematic investigated in batch culture. Experiment focus on the effect of some important affecting factors on photo-hydrogen production. Results indicated that immobilized Rhodopseudomonas faecalis RLD-53 exhibited the highest hydrogen yield of 3.15 mol H2/mol acetate under follow optimal condition: agar granule diameter of 2.5 mm, inoculum age of 24 h, agar concentration of 2%, biomass of 4 mg/ml in agar and light intensity of 9000 lux. More importantly, immobilized photo-fermentation bacteria not only can enhance hydrogen production but can increase acids-tolerance capacity, even at pH 5.0 hydrogen also was produced, and thus hopefully immobilized photo-fermentation bacteria can be applied in the combination of dark and photo-fermentation for hydrogen production with high yield.展开更多
文摘In this work, the optimization of hydrogen production by photo-fermentation bacteria immobilized on agar gel granule was systematic investigated in batch culture. Experiment focus on the effect of some important affecting factors on photo-hydrogen production. Results indicated that immobilized Rhodopseudomonas faecalis RLD-53 exhibited the highest hydrogen yield of 3.15 mol H2/mol acetate under follow optimal condition: agar granule diameter of 2.5 mm, inoculum age of 24 h, agar concentration of 2%, biomass of 4 mg/ml in agar and light intensity of 9000 lux. More importantly, immobilized photo-fermentation bacteria not only can enhance hydrogen production but can increase acids-tolerance capacity, even at pH 5.0 hydrogen also was produced, and thus hopefully immobilized photo-fermentation bacteria can be applied in the combination of dark and photo-fermentation for hydrogen production with high yield.
