Drought stress impairs crop growth and development.BEL1-like family transcription factors may be involved in plant response to drought stress,but little is known of the molecular mechanism by which these proteins regu...Drought stress impairs crop growth and development.BEL1-like family transcription factors may be involved in plant response to drought stress,but little is known of the molecular mechanism by which these proteins regulate plant response and defense to drought stress.Here we show that the BEL1-like transcription factor GhBLH5-A05 functions in cotton(Gossypium hirsutum)response and defense to drought stress.Expression of GhBLH5-A05 in cotton was induced by drought stress.Overexpression of GhBLH5-A05 in both Arabidopsis and cotton increased drought tolerance,whereas silencing GhBLH5-A05 in cotton resulted in elevated sensitivity to drought stress.GhBLH5-A05 binds to cis elements in the promoters of GhRD20-A09 and GhDREB2C-D05 to activate the expression of these genes.GhBLH5-A05 interacted with the KNOX transcription factor GhKNAT6-A03.Co-expression of GhBLH5-A05 and GhKNAT6-A03 increased the transcription of GhRD20-A09 and GhDREB2C-D05.We conclude that GhBLH5-A05 acts as a regulatory factor with GhKNAT6-A03 functioning in cotton response to drought stress by activating the expression of the drought-responsive genes GhRD20-A09 and GhDREB2C-D05.展开更多
在大豆(Glycine max L.Merr.)中克隆了一个1008bp的表皮毛相关基因GmTTG1-like(Glycine max Transparent Testa Glabra 1-like);生物信息学分析表明,GmTTG1-like蛋白在结构上具有4个WD40重复(WD40-repeat)结构域,与烟草中NtTTG2(Nicotia...在大豆(Glycine max L.Merr.)中克隆了一个1008bp的表皮毛相关基因GmTTG1-like(Glycine max Transparent Testa Glabra 1-like);生物信息学分析表明,GmTTG1-like蛋白在结构上具有4个WD40重复(WD40-repeat)结构域,与烟草中NtTTG2(Nicotiana tabacum Testa Glabra 2)具有较高同源性。推测GmTTG1-like与大豆表皮毛的生物学功能有关,并可能作用于大豆发育和防卫反应过程。采用RTPCR和q-RT-PCR两种方法检测GmTTG1-like在植物根、茎、叶、花和种子中的表达。构建GmTTG1-like植物表达载体并获得可以侵染的农杆菌阳性克隆。展开更多
SOC1/TM3(SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1/Tomato MADS-box gene 3)是MADS-box家族一员,它能够整合多条开花途径的开花信号,调节植物由营养生长向生殖生长的转变。在从梅花长蕊绿萼品种中克隆到3个SOC1同源基因PmSOC1-1、P...SOC1/TM3(SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1/Tomato MADS-box gene 3)是MADS-box家族一员,它能够整合多条开花途径的开花信号,调节植物由营养生长向生殖生长的转变。在从梅花长蕊绿萼品种中克隆到3个SOC1同源基因PmSOC1-1、PmSOC1-2和PmSOC1-3的基础上,构建由CaMV35S启动子驱动的植物表达载体,并在拟南芥中过量表达,以研究其功能。通过对转基因植株表型观察发现,3个PmSOC1-like基因都具有使野生型拟南芥提前开花的作用。其中,PmSOC1-2作用最强,PmSOC1-1居中,PmSOC1-3作用最弱。对转基因拟南芥内源成花基因的qRT-PCR检测说明,PmSOC1-like基因主要是通过上调其下游花分生组织特性基因(AGL24、LFY、AP1和FUL)来促进植物开花;此外,PmSOC1-1和PmSOC1-2还导致了花瓣细丝状、花萼叶片化、花瓣和花萼宿存等花器官形态的改变,说明它们可能还具有影响花器官发育的功能。研究结果将有助于阐明梅花由营养生长向生殖生长转变的分子机制。展开更多
Physarum polycephalum L., a naturally synchronized myxomycophyta, was demonstrated to contain a cyclin B1-like protein by Western blot and immunoelectron microscopy. The content and subcellular location of the protein...Physarum polycephalum L., a naturally synchronized myxomycophyta, was demonstrated to contain a cyclin B1-like protein by Western blot and immunoelectron microscopy. The content and subcellular location of the protein varied during the cell cycle. The cyclin B1-like protein was first detected in the plasmodia of S phase while it did not appear in the nuclei until late G2 phase. The content of the protein in both the plasmodia and nuclei rose gradually onwards, peaked at metaphase and disappeared abruptly at ana-telophase. The protein was found to be distributed in both the cytoplasm and nuclei in late G2 phase and metaphase. In nuclei, the protein was mainly located in the chromosomal and nucleolar areas. The results suggest that the cyclin B1-like protein of P. polycephalum begins to be synthesized at S phase, enters the nuclei at late G2 phase, accumulates in both cytoplasm and nuclei onwards and breaks down at ana-telophase. The results also suggest that the cyclin B1-like protein acts as a cytoplasmic-nuclear protein during certain phases of the cell cycle.展开更多
为了研究CR1-like基因的单核苷酸多态性（single nucleotide polymorphism,SNP）和拷贝数变异（copy number variation,CNV）,分析CR1-like的基因与蛋白质表达量、基因与蛋白质分子质量之间的关系,试验选择41头长白猪,采用直接测序和等...为了研究CR1-like基因的单核苷酸多态性（single nucleotide polymorphism,SNP）和拷贝数变异（copy number variation,CNV）,分析CR1-like的基因与蛋白质表达量、基因与蛋白质分子质量之间的关系,试验选择41头长白猪,采用直接测序和等位基因特异性PCR（Allele-specific PCR,AS-PCR）技术检测了CR1-like基因的SNP;用实时荧光定量PCR（Real-time PCR）技术和2-ΔΔCt相对定量分析方法检测了CR1-like基因的CNV,并利用一般线性模型（general linear model,GLM）和相关分析检验了CR1-like基因多态性与猪红细胞CR1-like蛋白质性状的关联性。结果表明：CR1-like基因存在CNV,且长白猪CR1-like基因以拷贝数正常为主,占总数的68.30%,拷贝数缺失占总数的31.70%,拷贝正常数和拷贝缺失数间差异极显著（P〈0.01）;CR1-like基因外显子上检测到8个SNP位点,rs321524247和rs330380626符合哈代-温伯格平衡且处于完全连锁（D＇=1,r2=1）,其中单倍型AT为主要单倍型,频率为0.915。说明CR1-like基因拷贝数对猪红细胞CR1-like蛋白质表达量有极显著影响。展开更多
基金supported by the Project from the Ministry of Agriculture of China for Transgenic Research(2014ZX0800927B)the National Natural Science Foundation of China(31871667).
文摘Drought stress impairs crop growth and development.BEL1-like family transcription factors may be involved in plant response to drought stress,but little is known of the molecular mechanism by which these proteins regulate plant response and defense to drought stress.Here we show that the BEL1-like transcription factor GhBLH5-A05 functions in cotton(Gossypium hirsutum)response and defense to drought stress.Expression of GhBLH5-A05 in cotton was induced by drought stress.Overexpression of GhBLH5-A05 in both Arabidopsis and cotton increased drought tolerance,whereas silencing GhBLH5-A05 in cotton resulted in elevated sensitivity to drought stress.GhBLH5-A05 binds to cis elements in the promoters of GhRD20-A09 and GhDREB2C-D05 to activate the expression of these genes.GhBLH5-A05 interacted with the KNOX transcription factor GhKNAT6-A03.Co-expression of GhBLH5-A05 and GhKNAT6-A03 increased the transcription of GhRD20-A09 and GhDREB2C-D05.We conclude that GhBLH5-A05 acts as a regulatory factor with GhKNAT6-A03 functioning in cotton response to drought stress by activating the expression of the drought-responsive genes GhRD20-A09 and GhDREB2C-D05.
文摘SOC1/TM3(SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1/Tomato MADS-box gene 3)是MADS-box家族一员,它能够整合多条开花途径的开花信号,调节植物由营养生长向生殖生长的转变。在从梅花长蕊绿萼品种中克隆到3个SOC1同源基因PmSOC1-1、PmSOC1-2和PmSOC1-3的基础上,构建由CaMV35S启动子驱动的植物表达载体,并在拟南芥中过量表达,以研究其功能。通过对转基因植株表型观察发现,3个PmSOC1-like基因都具有使野生型拟南芥提前开花的作用。其中,PmSOC1-2作用最强,PmSOC1-1居中,PmSOC1-3作用最弱。对转基因拟南芥内源成花基因的qRT-PCR检测说明,PmSOC1-like基因主要是通过上调其下游花分生组织特性基因(AGL24、LFY、AP1和FUL)来促进植物开花;此外,PmSOC1-1和PmSOC1-2还导致了花瓣细丝状、花萼叶片化、花瓣和花萼宿存等花器官形态的改变,说明它们可能还具有影响花器官发育的功能。研究结果将有助于阐明梅花由营养生长向生殖生长转变的分子机制。
文摘Physarum polycephalum L., a naturally synchronized myxomycophyta, was demonstrated to contain a cyclin B1-like protein by Western blot and immunoelectron microscopy. The content and subcellular location of the protein varied during the cell cycle. The cyclin B1-like protein was first detected in the plasmodia of S phase while it did not appear in the nuclei until late G2 phase. The content of the protein in both the plasmodia and nuclei rose gradually onwards, peaked at metaphase and disappeared abruptly at ana-telophase. The protein was found to be distributed in both the cytoplasm and nuclei in late G2 phase and metaphase. In nuclei, the protein was mainly located in the chromosomal and nucleolar areas. The results suggest that the cyclin B1-like protein of P. polycephalum begins to be synthesized at S phase, enters the nuclei at late G2 phase, accumulates in both cytoplasm and nuclei onwards and breaks down at ana-telophase. The results also suggest that the cyclin B1-like protein acts as a cytoplasmic-nuclear protein during certain phases of the cell cycle.
文摘为了研究CR1-like基因的单核苷酸多态性（single nucleotide polymorphism,SNP）和拷贝数变异（copy number variation,CNV）,分析CR1-like的基因与蛋白质表达量、基因与蛋白质分子质量之间的关系,试验选择41头长白猪,采用直接测序和等位基因特异性PCR（Allele-specific PCR,AS-PCR）技术检测了CR1-like基因的SNP;用实时荧光定量PCR（Real-time PCR）技术和2-ΔΔCt相对定量分析方法检测了CR1-like基因的CNV,并利用一般线性模型（general linear model,GLM）和相关分析检验了CR1-like基因多态性与猪红细胞CR1-like蛋白质性状的关联性。结果表明：CR1-like基因存在CNV,且长白猪CR1-like基因以拷贝数正常为主,占总数的68.30%,拷贝数缺失占总数的31.70%,拷贝正常数和拷贝缺失数间差异极显著（P〈0.01）;CR1-like基因外显子上检测到8个SNP位点,rs321524247和rs330380626符合哈代-温伯格平衡且处于完全连锁（D＇=1,r2=1）,其中单倍型AT为主要单倍型,频率为0.915。说明CR1-like基因拷贝数对猪红细胞CR1-like蛋白质表达量有极显著影响。
