[Objectives] The aim was to explore the best parameters for processing Aconiti Kusnezoffii Radix by bean curd. [Methods] Orthogonal test( L_(16)(4~3)) was designed to investigate the optimal soaking time,decocting tim...[Objectives] The aim was to explore the best parameters for processing Aconiti Kusnezoffii Radix by bean curd. [Methods] Orthogonal test( L_(16)(4~3)) was designed to investigate the optimal soaking time,decocting time and bean curd proportion for processing Aconiti Kusnezoffii Radix by bean curd. The contents of monoester alkaloids and diester-alkaloids were measured as the evaluation indexes. [Results]The contents of monoester alkaloids were 0. 11%,0. 07%,0. 062% and 0. 048% respectively under the four levels of soaking time,were0. 081%,0. 066%,0. 074% and 0. 067% respectively under the four levels of decocting time,and were 0. 070%,0. 072%,0. 080% and0. 073% respectively under the four levels of bean curd proportion. The content of diester-alkaloids was all below 0. 034%. The influence intensity of the three factors on the content of monoester alkaloids ranked as soaking time > decocting time > bean curd proportion. Under any of the above conditions,the content of diester-alkaloids was below the limit of Chinese Pharmacopoeia. The content of diester-alkaloids was reduced obviously with the increased use of bean curd. [Conclusions] The optimum processing conditions for Aconiti Kusnezoffii Radix were A_3B_3C_2,i. e.,soaking time of 6 d,decocting time of 5 h and bean curd proportion of 25%.展开更多
[Objectives]To establish a TLC qualitative method for the determination of Radix Aconiti,Radix Aconiti Kusnezoffii,Caesalpinia sappan L.and Radix zanthoxyli.[Methods]TLC was used to study the quality of Radix Aconiti,...[Objectives]To establish a TLC qualitative method for the determination of Radix Aconiti,Radix Aconiti Kusnezoffii,Caesalpinia sappan L.and Radix zanthoxyli.[Methods]TLC was used to study the quality of Radix Aconiti,Radix Aconiti Kusnezoffii and C.sappan L.in Xunxi Shujin Decoction,and the influencing factors were investigated to optimize the TLC conditions.[Results]In the TLC identification test of Radix Aconiti,Radix Aconiti Kusnezoffii,C.sappan L.and R.zanthoxyli in Xunxi Shujin Decoction,the spots were clear,the resolution was good,the specific value was moderate,the negative control was not disturbed,and the specificity was strong.[Conclusions]The established TLC method was simple,specific and reproducible,and could be used for the quality control of Xunxi Shujin Decoction.展开更多
目的研究诃子汤炮制草乌对草乌多成分体内动态变化过程的影响,从而探讨蒙医诃子汤炮制草乌机理。方法采用超高效液相色谱-串联飞行时间/质谱(Ultra performance liquid chromatogr time of flight/mass spectrometry,UPLC-Q-TOF/MS)技...目的研究诃子汤炮制草乌对草乌多成分体内动态变化过程的影响,从而探讨蒙医诃子汤炮制草乌机理。方法采用超高效液相色谱-串联飞行时间/质谱(Ultra performance liquid chromatogr time of flight/mass spectrometry,UPLC-Q-TOF/MS)技术和封闭肠环法,收集生草乌、炮制草乌及诃子的肠壁吸收、肠道菌吸收代谢以及肝代谢样品,分别建立大鼠含药血浆指纹图谱,对各个样品所含成分进行对比分析研究。结果在肠壁吸收研究中检测出生草乌、炮制草乌成分共32种,其中生草乌组、制草乌组共有成分16种、只在生草乌组检测出而未在制草乌组检测到成分8种、只在制草乌组检测出而未在生草乌组检测出成分8种,诃子成分17种。在肠道菌吸收代谢研究中检测出草乌31种成分,其中生草乌、制草乌组共有成分10种、只在生草乌组检测出而未在制草乌组检测到成分7种、只在制草乌组检测到而未在生草乌组检测出成分14种,诃子13种成分。在肝代谢研究中草乌检测出21种成分,其中生草乌组、制草乌组共有成分5种、只在生草乌组检测出而未在制草乌组检测到成分7种、只在制草乌组检测出而未在生草乌组检测出成分9种,诃子11种成分。对已鉴定出成分进行含量对比研究结果显示生草乌组与制草乌组相比较其成分在肠壁吸收中较多在肠道菌及肝中吸收代谢较少,而制草乌与诃子成分在肠道菌及肝代谢中较多,而在肠壁与肝代谢中较少。结论经过诃子汤炮制草乌可使草乌成分吸收代谢位点发生了变化,使草乌成分在肠壁吸收中变少而肠道菌及肝代谢中变多。由此可知,经诃子汤炮制使草乌成分的吸收变缓慢且代谢变快,因而可有效起到草乌中毒性成分被人体缓慢吸收且快速代谢。由此推断,诃子汤炮制草乌通过以下途径达到减毒目的:一方面避免草乌毒性成分吸收过快而导致血药浓度快速升高导致中毒;另一方面加速代谢毒性成分,进而降低血药浓度而避免中毒。展开更多
文摘[Objectives] The aim was to explore the best parameters for processing Aconiti Kusnezoffii Radix by bean curd. [Methods] Orthogonal test( L_(16)(4~3)) was designed to investigate the optimal soaking time,decocting time and bean curd proportion for processing Aconiti Kusnezoffii Radix by bean curd. The contents of monoester alkaloids and diester-alkaloids were measured as the evaluation indexes. [Results]The contents of monoester alkaloids were 0. 11%,0. 07%,0. 062% and 0. 048% respectively under the four levels of soaking time,were0. 081%,0. 066%,0. 074% and 0. 067% respectively under the four levels of decocting time,and were 0. 070%,0. 072%,0. 080% and0. 073% respectively under the four levels of bean curd proportion. The content of diester-alkaloids was all below 0. 034%. The influence intensity of the three factors on the content of monoester alkaloids ranked as soaking time > decocting time > bean curd proportion. Under any of the above conditions,the content of diester-alkaloids was below the limit of Chinese Pharmacopoeia. The content of diester-alkaloids was reduced obviously with the increased use of bean curd. [Conclusions] The optimum processing conditions for Aconiti Kusnezoffii Radix were A_3B_3C_2,i. e.,soaking time of 6 d,decocting time of 5 h and bean curd proportion of 25%.
基金Supported by Project of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine(GXZYZ20210082)Traditional Chinese Medicine Zhuang and Yao Medicine Hospital Preparation Quality Improvement Project of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine(GZZJ202002).
文摘[Objectives]To establish a TLC qualitative method for the determination of Radix Aconiti,Radix Aconiti Kusnezoffii,Caesalpinia sappan L.and Radix zanthoxyli.[Methods]TLC was used to study the quality of Radix Aconiti,Radix Aconiti Kusnezoffii and C.sappan L.in Xunxi Shujin Decoction,and the influencing factors were investigated to optimize the TLC conditions.[Results]In the TLC identification test of Radix Aconiti,Radix Aconiti Kusnezoffii,C.sappan L.and R.zanthoxyli in Xunxi Shujin Decoction,the spots were clear,the resolution was good,the specific value was moderate,the negative control was not disturbed,and the specificity was strong.[Conclusions]The established TLC method was simple,specific and reproducible,and could be used for the quality control of Xunxi Shujin Decoction.
文摘目的研究诃子汤炮制草乌对草乌多成分体内动态变化过程的影响,从而探讨蒙医诃子汤炮制草乌机理。方法采用超高效液相色谱-串联飞行时间/质谱(Ultra performance liquid chromatogr time of flight/mass spectrometry,UPLC-Q-TOF/MS)技术和封闭肠环法,收集生草乌、炮制草乌及诃子的肠壁吸收、肠道菌吸收代谢以及肝代谢样品,分别建立大鼠含药血浆指纹图谱,对各个样品所含成分进行对比分析研究。结果在肠壁吸收研究中检测出生草乌、炮制草乌成分共32种,其中生草乌组、制草乌组共有成分16种、只在生草乌组检测出而未在制草乌组检测到成分8种、只在制草乌组检测出而未在生草乌组检测出成分8种,诃子成分17种。在肠道菌吸收代谢研究中检测出草乌31种成分,其中生草乌、制草乌组共有成分10种、只在生草乌组检测出而未在制草乌组检测到成分7种、只在制草乌组检测到而未在生草乌组检测出成分14种,诃子13种成分。在肝代谢研究中草乌检测出21种成分,其中生草乌组、制草乌组共有成分5种、只在生草乌组检测出而未在制草乌组检测到成分7种、只在制草乌组检测出而未在生草乌组检测出成分9种,诃子11种成分。对已鉴定出成分进行含量对比研究结果显示生草乌组与制草乌组相比较其成分在肠壁吸收中较多在肠道菌及肝中吸收代谢较少,而制草乌与诃子成分在肠道菌及肝代谢中较多,而在肠壁与肝代谢中较少。结论经过诃子汤炮制草乌可使草乌成分吸收代谢位点发生了变化,使草乌成分在肠壁吸收中变少而肠道菌及肝代谢中变多。由此可知,经诃子汤炮制使草乌成分的吸收变缓慢且代谢变快,因而可有效起到草乌中毒性成分被人体缓慢吸收且快速代谢。由此推断,诃子汤炮制草乌通过以下途径达到减毒目的:一方面避免草乌毒性成分吸收过快而导致血药浓度快速升高导致中毒;另一方面加速代谢毒性成分,进而降低血药浓度而避免中毒。
