Matrix solid-phase dispersion（MSPD） was developed for the extraction of four alkaloids, including aconitine, mesaconitine, hypaconitine and deoxyaconitine, from the roots ofAconitum kusnezoffii Reichb. The determina...Matrix solid-phase dispersion（MSPD） was developed for the extraction of four alkaloids, including aconitine, mesaconitine, hypaconitine and deoxyaconitine, from the roots ofAconitum kusnezoffii Reichb. The determination of the analyte was carried out by high performance liquid chromatography with UV detection. The alkaline alumina was used as sorbent. The mixture of acetonitrile and water was used as elution solvent. Several extraction parameters, such as type of sorbent, the ratio of sample to solid support material, type of the elution solvent and the volume of the elution solvent were tested. Mean recoveries ranged from 93.16% to 102.73%, with relative standard deviations from 0.27% to 4.17%. With the extraction efficiency and time expenditure taken into account, MSPD extraction should be a comparatively good method.展开更多
AIM: To look for new principles of high activity and low toxicity from the leaves of Aconitum kusnezoffii Reichb. METHODS: The constituents were separated by using various column chromatography and their sturctures we...AIM: To look for new principles of high activity and low toxicity from the leaves of Aconitum kusnezoffii Reichb. METHODS: The constituents were separated by using various column chromatography and their sturctures were identified on the basis of spectral analysis. RESULTS: Three compounds were isolated from the leaves of Aconitum kusnezoffii . Their structures were elucidated as beiwutine (I). 8 OEt 14 benzoylmesaconine (II) and beiwucine (III). CONCLUSION: Compound III was shown to be a new diterpenoid alkaloid.展开更多
基金Supported by the Projects in the National Science & Technology Pillar Program During the Eleventh Five-Year Plan Period of China(No 2006BAI14B01)
文摘Matrix solid-phase dispersion（MSPD） was developed for the extraction of four alkaloids, including aconitine, mesaconitine, hypaconitine and deoxyaconitine, from the roots ofAconitum kusnezoffii Reichb. The determination of the analyte was carried out by high performance liquid chromatography with UV detection. The alkaline alumina was used as sorbent. The mixture of acetonitrile and water was used as elution solvent. Several extraction parameters, such as type of sorbent, the ratio of sample to solid support material, type of the elution solvent and the volume of the elution solvent were tested. Mean recoveries ranged from 93.16% to 102.73%, with relative standard deviations from 0.27% to 4.17%. With the extraction efficiency and time expenditure taken into account, MSPD extraction should be a comparatively good method.
文摘AIM: To look for new principles of high activity and low toxicity from the leaves of Aconitum kusnezoffii Reichb. METHODS: The constituents were separated by using various column chromatography and their sturctures were identified on the basis of spectral analysis. RESULTS: Three compounds were isolated from the leaves of Aconitum kusnezoffii . Their structures were elucidated as beiwutine (I). 8 OEt 14 benzoylmesaconine (II) and beiwucine (III). CONCLUSION: Compound III was shown to be a new diterpenoid alkaloid.
