Objective:Using network pharmacology to explore the target and mechanism of Chuanxiong Rhizoma and Acori Tatarinowii Rhizoma in the treatment of vascular dementia(VaD),so as to provide a reference for treating VaD thr...Objective:Using network pharmacology to explore the target and mechanism of Chuanxiong Rhizoma and Acori Tatarinowii Rhizoma in the treatment of vascular dementia(VaD),so as to provide a reference for treating VaD through them.Methods:Traditional Chinese medicine systems pharmacology database and analysis platform were used to screen the main active ingredients and targets of Chuanxiong Rhizoma and Acori Tatarinowii Rhizoma.By means of Gene Cards and Online Mendelian Inheritance in Man(OMIM),targets of VaD were collected.The intersecting targets were obtained by using the Venn map.The String online database was used to build a protein-protein interactions Network and the Metascape database was used to perform GO function enrichment analysis and KEGG pathway enrichment analysis.A“drug-ingredient-target-pathway”network was constructed by Cytoscape software.Autodock vina software was used to conduct molecular docking between targets.Results:A total of 7 active ingredients in Chuanxiong Rhizoma and 4 active ingredients in Acori Tatarinowii Rhizoma were screened.There were 42 active targets of Chuanxiong Rhizoma and 70 active targets of Acori Tatarinowii Rhizoma and 1152 disease targets.After deleting the repeat value,51 drugs targets were obtained.After the intersection,with a total of 25 targets.According to GO and KEGG enrichment analysis,the main biological processes involved include cellular response to lipid,negative regulation of apoptotic signaling pathway,blood circulation,response to a steroid hormone,etc.The main pathways include pathways in cancer,PI3K-Akt signaling pathway,and AGE-RAGE signaling pathway in diabetic complications,etc.Molecular docking showed that the most active docking combinations were AKT1 and Perlolyrine,RELA and FA,MAPK14 and FA,respectively.Conclusion:Chuanxiong Rhizoma and Acori Tatarinowii Rhizoma play an important role in the treatment of VaD mainly by anti-inflammatory and anti-apoptosis.展开更多
Extracts from Rhizoma Acori Tatarinowii (Grassleaf Sweetflag Rhizome,Shichangpu) have been shown to improve learning and memory,reduce anxiety,allay excitement,and suppress seizures.Rhizoma Acori Tatarinowii extracts ...Extracts from Rhizoma Acori Tatarinowii (Grassleaf Sweetflag Rhizome,Shichangpu) have been shown to improve learning and memory,reduce anxiety,allay excitement,and suppress seizures.Rhizoma Acori Tatarinowii extracts interact with γ-aminobutyric acid and activate the γ-aminobutyric acid type A receptor,although few studies have addressed the precise effects of γ-aminobutyric acid type A receptor α1 subunit.In the present study,γ-aminobutyric acid type A receptor α1 subunit protein expression in the cerebral cortex and hippocampus,and pathological scores of brain injury,were significantly greater following recurrent seizures,but significantly decreased following treatment with Rhizoma Acori Tatarinowii extracts.These results indicated that Rhizoma Acori Tatarinowii extracts down-regulated γ-aminobutyric acid type A receptor α1 subunit protein expression in the cerebral cortex and hippocampus and protected seizure-induced brain injury during development.展开更多
[Objectives]This paper aims to establish a method to simultaneously determine the content ofβ-asarone in Rhizoma Acori Tatarinowii dried by three different methods.[Methods]Reversed-phase high-performance liquid chro...[Objectives]This paper aims to establish a method to simultaneously determine the content ofβ-asarone in Rhizoma Acori Tatarinowii dried by three different methods.[Methods]Reversed-phase high-performance liquid chromatography was used,and the chromatographic conditions were as follows:column,Thermo SCIENTIFIC Hypersil GOLD Dim.(mm);mobile phase,methanol-0.1%phosphoric acid(63∶37);column temperature,30℃;flow rate,1mL/min;detection wavelength,257 nm;sample size,10μL.[Results]The linear range of the injection volume ofβ-asarone was 49.28-246.40μg/mL(R=0.9993);the limit of quantification was 0.85 ng and the detection limit was 0.34 ng;the RSD values of precision,stability and reproducibility tests were all less than 3%;and the sample recovery rate was 98.53%-98.97%(RSD<3.00).The results show that the content ofβ-asarone was highest in shade-dried Rhizoma Acori Tatarinowii.The order ofβ-asarone content was as follows:Rhizoma Acori Tatarinowii dried in shade>Rhizoma Acori Tatarinowii dried at 55℃>Rhizoma Acori Tatarinowii dried at 60℃.[Conclusions]This method is sensitive,reliable,and reproducible.It can be used to simultaneously determine the content ofβ-asarone in Rhizoma Acori Tatarinowii dried by three different methods.展开更多
OBJECTIVE:To investigate the mechanism underlying the effects of Shichangpu(Rhizoma Acori Tatarinowii)on attention deficit hyperactivity disorder(ADHD).METHODS:A network pharmacology approach integrating ingredients o...OBJECTIVE:To investigate the mechanism underlying the effects of Shichangpu(Rhizoma Acori Tatarinowii)on attention deficit hyperactivity disorder(ADHD).METHODS:A network pharmacology approach integrating ingredients of Shichangpu(Rhizoma Acori Tatarinowii)and target with ADHD,network construction,molecular function interactions and pathway analysis was used.RESULTS:This approach successfully helped to identify 7 active ingredients of CN,interacting with21 key targets(ADRA1 A,ADRA1 B,ADRA2 A,ADRA2 B,ADRA2 C,ADRB1,ADRB2,CHRM1,CHRM2,CHRM3,PTGS1,SLC6 A2,SLC6 A3,SLC6 A4,DRD1,DRD5,HTR2 A,ADRA1 D,MAOB,GRIA2,HTR1 A).The molecular function interactions among candidate targets mainly consisted of four groups:G-protein coupled amine receptor activity,catecholamine binding,monoamine transmembrane transporter activity and neurotransmitter receptor activity.Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that Shichangpu(Rhizoma Acori Tatarinowii)-regulated pathways were mainly classified into signal transduction and monoamine neurotransmitters.CONCLUSION:Our investigation revealed that Shichangpu(Rhizoma Acori Tatarinowii)could improve the symptoms of ADHD by regulating neurotransmitter,in multiple types of compounds-target-pathway,which may be implicated in the major pathological processes of ADHD.展开更多
基金supported by National Key R&D Program of(China2019YFC1708502).
文摘Objective:Using network pharmacology to explore the target and mechanism of Chuanxiong Rhizoma and Acori Tatarinowii Rhizoma in the treatment of vascular dementia(VaD),so as to provide a reference for treating VaD through them.Methods:Traditional Chinese medicine systems pharmacology database and analysis platform were used to screen the main active ingredients and targets of Chuanxiong Rhizoma and Acori Tatarinowii Rhizoma.By means of Gene Cards and Online Mendelian Inheritance in Man(OMIM),targets of VaD were collected.The intersecting targets were obtained by using the Venn map.The String online database was used to build a protein-protein interactions Network and the Metascape database was used to perform GO function enrichment analysis and KEGG pathway enrichment analysis.A“drug-ingredient-target-pathway”network was constructed by Cytoscape software.Autodock vina software was used to conduct molecular docking between targets.Results:A total of 7 active ingredients in Chuanxiong Rhizoma and 4 active ingredients in Acori Tatarinowii Rhizoma were screened.There were 42 active targets of Chuanxiong Rhizoma and 70 active targets of Acori Tatarinowii Rhizoma and 1152 disease targets.After deleting the repeat value,51 drugs targets were obtained.After the intersection,with a total of 25 targets.According to GO and KEGG enrichment analysis,the main biological processes involved include cellular response to lipid,negative regulation of apoptotic signaling pathway,blood circulation,response to a steroid hormone,etc.The main pathways include pathways in cancer,PI3K-Akt signaling pathway,and AGE-RAGE signaling pathway in diabetic complications,etc.Molecular docking showed that the most active docking combinations were AKT1 and Perlolyrine,RELA and FA,MAPK14 and FA,respectively.Conclusion:Chuanxiong Rhizoma and Acori Tatarinowii Rhizoma play an important role in the treatment of VaD mainly by anti-inflammatory and anti-apoptosis.
基金supported by the Natural Science Foundation of Hunan Province (Effects and mechanisms of γ-aminobutyric acid type A receptor agonist on brain injury in the development stage),No.09JJ6032
文摘Extracts from Rhizoma Acori Tatarinowii (Grassleaf Sweetflag Rhizome,Shichangpu) have been shown to improve learning and memory,reduce anxiety,allay excitement,and suppress seizures.Rhizoma Acori Tatarinowii extracts interact with γ-aminobutyric acid and activate the γ-aminobutyric acid type A receptor,although few studies have addressed the precise effects of γ-aminobutyric acid type A receptor α1 subunit.In the present study,γ-aminobutyric acid type A receptor α1 subunit protein expression in the cerebral cortex and hippocampus,and pathological scores of brain injury,were significantly greater following recurrent seizures,but significantly decreased following treatment with Rhizoma Acori Tatarinowii extracts.These results indicated that Rhizoma Acori Tatarinowii extracts down-regulated γ-aminobutyric acid type A receptor α1 subunit protein expression in the cerebral cortex and hippocampus and protected seizure-induced brain injury during development.
基金Key Research and Development Project of Department of Science and Technology of Guangxi Zhuang Autonomous Region(AB19110003)Project for Improving Basic Scientific Research Ability of Young and Middle-aged Teachers in Colleges and Universities of Guangxi(2019KY0341,2019ky0344)+2 种基金Open Project of Guangxi Zhuang Yao Medicine Center of Engineering and Technology(KJT1900105)Youth Foundation of Guangxi University of Chinese Medicine(2019QN036,2019QN030)Traditional Chinese Medicine Scientific Research Laboratory(Grade III)of National Administration of Traditional Chinese Medicine:Laboratory of Chinese(Zhuang)Medicine Chemical and Quality Analysis(Guo Zhong Yi Yao Fa 2009[21]).
文摘[Objectives]This paper aims to establish a method to simultaneously determine the content ofβ-asarone in Rhizoma Acori Tatarinowii dried by three different methods.[Methods]Reversed-phase high-performance liquid chromatography was used,and the chromatographic conditions were as follows:column,Thermo SCIENTIFIC Hypersil GOLD Dim.(mm);mobile phase,methanol-0.1%phosphoric acid(63∶37);column temperature,30℃;flow rate,1mL/min;detection wavelength,257 nm;sample size,10μL.[Results]The linear range of the injection volume ofβ-asarone was 49.28-246.40μg/mL(R=0.9993);the limit of quantification was 0.85 ng and the detection limit was 0.34 ng;the RSD values of precision,stability and reproducibility tests were all less than 3%;and the sample recovery rate was 98.53%-98.97%(RSD<3.00).The results show that the content ofβ-asarone was highest in shade-dried Rhizoma Acori Tatarinowii.The order ofβ-asarone content was as follows:Rhizoma Acori Tatarinowii dried in shade>Rhizoma Acori Tatarinowii dried at 55℃>Rhizoma Acori Tatarinowii dried at 60℃.[Conclusions]This method is sensitive,reliable,and reproducible.It can be used to simultaneously determine the content ofβ-asarone in Rhizoma Acori Tatarinowii dried by three different methods.
基金Supported by the National Natural Science Foundation of China(No.81774366)National Major New Drug Creation Project(No.2014ZX09304-306-002).
文摘OBJECTIVE:To investigate the mechanism underlying the effects of Shichangpu(Rhizoma Acori Tatarinowii)on attention deficit hyperactivity disorder(ADHD).METHODS:A network pharmacology approach integrating ingredients of Shichangpu(Rhizoma Acori Tatarinowii)and target with ADHD,network construction,molecular function interactions and pathway analysis was used.RESULTS:This approach successfully helped to identify 7 active ingredients of CN,interacting with21 key targets(ADRA1 A,ADRA1 B,ADRA2 A,ADRA2 B,ADRA2 C,ADRB1,ADRB2,CHRM1,CHRM2,CHRM3,PTGS1,SLC6 A2,SLC6 A3,SLC6 A4,DRD1,DRD5,HTR2 A,ADRA1 D,MAOB,GRIA2,HTR1 A).The molecular function interactions among candidate targets mainly consisted of four groups:G-protein coupled amine receptor activity,catecholamine binding,monoamine transmembrane transporter activity and neurotransmitter receptor activity.Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that Shichangpu(Rhizoma Acori Tatarinowii)-regulated pathways were mainly classified into signal transduction and monoamine neurotransmitters.CONCLUSION:Our investigation revealed that Shichangpu(Rhizoma Acori Tatarinowii)could improve the symptoms of ADHD by regulating neurotransmitter,in multiple types of compounds-target-pathway,which may be implicated in the major pathological processes of ADHD.
