Objective This meta-analysis explored whether the expression of actin filament-associated protein 1 antisense RNA 1(AFAP1-AS1)is related to the prognosis and clinicopathological features of patients with cancer.Method...Objective This meta-analysis explored whether the expression of actin filament-associated protein 1 antisense RNA 1(AFAP1-AS1)is related to the prognosis and clinicopathological features of patients with cancer.Methods PubMed,EMBASE,and Cochrane Library were systematically searched.Hazard ratios(HRs)with 95%confidence intervals(CIs)were used to assess the prognostic value based on overall survival(OS),disease-free survival(DFS),and progression-free survival(PFS).Odds ratios(ORs)with 95%CIs were used to determine the relationships between AFAP1-AS1 and clinicopathological features,such as large tumor size(LTS),high tumor stage(HTS),poor histological grade(PHG),lymph node metastasis(LNM),and distant metastasis(DM).Results Thirty-five eligible articles and 3433 cases were analyzed.High AFAP1-AS1 expression,compared to low AFAP1-AS1 expression,correlated with significantly shorter OS(HR=2.15,95%CI=1.97-2.34,P<0.001),DFS(HR=1.37,95%CI=1.19-1.57,P<0.001),and PFS(HR=1.97,95%CI=1.56-2.50,P<0.001)in patients with cancer.In various cancers,elevated AFAP1-AS1 expression was significantly associated with LTS(OR=2.76,95%CI=2.16-3.53,P<0.001),HTS(OR=2.23,95%CI=1.83-2.71,P<0.001),and PHG(OR=1.39,95%CI=1.08-1.79,P=0.01)but not LNM(OR=1.59,95%CI=0.88-2.85,P=0.12)or DM(OR=1.81,95%CI=0.90-3.66,P=0.10).Conclusion High AFAP1-AS1 expression was associated with prognostic and clinicopathological features,suggesting that AFAP1-AS1 is a prognostic biomarker for human cancers.展开更多
【目的】观察苦瓜皂苷G对糖尿病肾病(DN)大鼠的治疗作用及机制。【方法】从55只大鼠中随机抽取45只采用一次性腹腔注射链脲佐菌素(STZ)法诱导DN模型。将造模成功的38只大鼠随机分为模型组9只、苦瓜皂苷G低剂量组9只、苦瓜皂苷G中剂量组1...【目的】观察苦瓜皂苷G对糖尿病肾病(DN)大鼠的治疗作用及机制。【方法】从55只大鼠中随机抽取45只采用一次性腹腔注射链脲佐菌素(STZ)法诱导DN模型。将造模成功的38只大鼠随机分为模型组9只、苦瓜皂苷G低剂量组9只、苦瓜皂苷G中剂量组10只、苦瓜皂苷G高剂量组10只。剩余10只大鼠设为正常组。对应灌胃给药4周后,酶联免疫吸附分析(ELISA)检测大鼠24 h尿蛋白水平,血糖仪检测空腹血糖(FBG),全自动生化分析仪检测血肌酐(SCr)、血尿素氮(BUN)水平,苏木素-伊红染色(HE)法观察大鼠肾组织病理学变化,采用实时定量聚合酶链反应(RT-qPCR)和Western Blot法分别检测E-钙黏蛋白(E-cad)、α-平滑肌肌动蛋白(α-SMA)、Notch1、锌指蛋白转录因子1(Snail1)、Jagged1 m RNA及蛋白表达情况。【结果】与正常组比较,模型组24 h尿蛋白、FBG、血清SCr和BUN水平及肾组织α-SMA、Notch1、Snail1、Jagged1 mRNA及蛋白水平显著升高,肾组织E-cad m RNA及蛋白水平降低,肾组织可见肾小球萎缩、肾小管扩张及间质纤维化;与模型组比较,苦瓜皂苷G低、中、高剂量组24 h尿蛋白、FBG、血清SCr和BUN水平及肾组织α-SMA、Notch1、Snail1、Jagged1 mRNA及蛋白水平显著降低,肾组织E-cad m RNA及蛋白水平升高,肾组织病理损伤得到改善。【结论】苦瓜皂苷G可有效改善DN大鼠肾功能障碍、减轻肾脏纤维化,其机制可能与通过调控Notch/Snail1信号通路,抑制α-SMA和Jagged1 mRNA及蛋白表达,增强E-cad mRNA及蛋白表达有关。展开更多
BACKGROUND: We previously showed that insulin-like growth factor binding protein-related protein 1 (IGFBPrP1) is a novel mediator in liver fibrosis. Transforming growth factor beta 1 (TGF beta 1) is known as the stron...BACKGROUND: We previously showed that insulin-like growth factor binding protein-related protein 1 (IGFBPrP1) is a novel mediator in liver fibrosis. Transforming growth factor beta 1 (TGF beta 1) is known as the strongest effector of liver fibrosis. Therefore, we aimed to investigate the detailed interaction between IGFBPrP1 and TGF beta 1 in primary hepatic stellate cells (HSCs). METHODS: We overexpressed TGF beta 1 or IGFBPrP1 and inhibited TGF beta 1 expression in primary HSCs for 6, 12, 24, 48, 72, and 96 hours to investigate their interaction and observe the accompanying expressions of a-smooth muscle actin (alpha-SMA), collagen I, fibronectin, and phosphorylated-mothers against decapentaplegic homolog 2/3 (p-Smad2/3). RESULTS: We found that the adenovirus vector encoding the TGF beta 1 gene (AdTGF beta 1) induced IGFBPrP1 expression while that of alpha-SMA, collagen I, fibronectin, and TGF beta 1 increased gradually. Concomitantly, AdIGFBPrP1 upregulated TGF beta 1, alpha-SMA, collagen I, fibronectin, and p-Smad2/3 in a time-dependent manner while IGFBPrP1 expression was decreased at 96 hours. Inhibition of TGF beta 1 expression reduced the IGFBPrP1-stimulated expression of alpha-SMA, collagen I, fibronectin, and p-Smad2/3. CONCLUSIONS: These findings for the first time suggest the existence of a possible mutually regulation between IGFBPrP1 and TGF beta 1, which likely accelerates liver fibrosis progression. Furthermore, IGFBPrP1 likely participates in liver fibrosis in a TGF beta 1-depedent manner, and may act as an upstream regulatory factor of TGF beta 1 in the Smad pathway.展开更多
Cardiotrophin-1 （CT-1） activates a distinct form of cardiac muscle cell hypertrophy in which the sarcomeric units are assembled in series. The aim of the study was to determine the expres- sion pattern of sarcomeric...Cardiotrophin-1 （CT-1） activates a distinct form of cardiac muscle cell hypertrophy in which the sarcomeric units are assembled in series. The aim of the study was to determine the expres- sion pattern of sarcomeric contractile protein α-actin, specialized eytoskeletal protein α-actinin and mitochondrial uncoupling protein-2 （UCP2） in myocardial remodeling induced by chronic exposure to CT-1. Kunming mice were intraperitoneally injected with carboxy-terminal polypeptide （CP） of CT-1 （CT-1-CP, 500 μg·kg-1·day^-1） for 1, 2, 3 and 4 week （s）, respectively （4 groups obtained according to the injection time, n=10 each, with 5 males and 5 females in each group）, Those injected with physiological saline for 4 weeks served as controls （n=10, with 5 males and 5 females）. The heart tissues of mice were harvested at 1, 2, 3 or 4 week （s）. Immunohistochemistry （IHC） and Western blotting （WB） were used to detect the distribution and expression of sarcomeric α-actin, α-aetinin and mitoehondrial UCP2 in myocardial tissues. IHC showed that α-actin was mainly distributed around the nuclei of cardiomyo- cytes, α-actinin concentrated around the striae and UCP2 scattered rather evenly in the plasma. The ex- pression of α-actin was slightly greater than that of α-actinin and UCP2 in the control group （IHC： χ^2=6.125; WB： F=0.249, P〉0.05） and it gradually decreased after exposure to CT-1-CP. There was no significant difference in the expression of α-actin between the control group and the CT-1-CP-treated groups （χ^2=7.386, P〉0.05）. But Western blotting revealed significant difference in the expression of α-actin between the control group and the 4-week CT-1-CP-treated group （F=2.912; q=4.203, P〈0.05）. Moreover, it was found that the expression of α-actinin increased stepwise with the exposure time in CT-1-CP-treated groups and differed significantly between CT-1-CP-treated groups and the control group （ICH： χ^2=21.977; WB： F=50.388; P〈0.01）. The expression of UCP2 was initially increased （WB： control group vs. 1- or 2-week group, q values： 5.603 and 9.995, respectively, P〈0.01） and then de- creased （WB： control group vs. 3-week group, q=4.742, P〈0.01; control group vs. 4-week group, q=0.558, P〉0.05）. It was suggested that long-term exposure to CT-1-CP could lead to the alteration in the expression of sarcomeric α-actin, α-actinin and mitochonclrial UCP2. The different expressions of sarcomeric structure proteins and mitochondrial UCP2 may be involved in myocardial remodeling.展开更多
目的:检测肌动蛋白丝相关蛋白1相似蛋白(actin filament-associated protein 1-like 2,A FA P-1L2)在不同分化程度胰腺癌细胞株中的表达,并观察其对下游磷脂酰肌醇3激酶/蛋白激酶(phosphatidylinositol 3 kinase/protein kinase B,PI3K/...目的:检测肌动蛋白丝相关蛋白1相似蛋白(actin filament-associated protein 1-like 2,A FA P-1L2)在不同分化程度胰腺癌细胞株中的表达,并观察其对下游磷脂酰肌醇3激酶/蛋白激酶(phosphatidylinositol 3 kinase/protein kinase B,PI3K/Akt)通路调控作用及对胰腺癌细胞的增殖、周期及凋亡的影响.方法:以Western blot及实时定量PCR检测(real-time quantitative PCR,q RT-PCR)法对不同分化程度胰腺癌细胞系PANC-1、Mia Pa Ca-2、Colo-357、BXPC-3、SW1990及CFPAC-1中AFAP-1L2表达进行检测;构建靶向AFAP-1L2的干扰质粒si AFAP-1L2,转染Mia Pa Ca-2细胞,以Western blot及q RTPCR法检测AFAP-1L2下调后PI3K/Akt通路蛋白及m RNA变化;四甲基偶氮唑盐微量酶反应比色法(methyl-thiazolyl-tetrazolium,MTT)法检测转染后Mia Pa Ca-2细胞增殖;流式细胞术检测细胞周期及凋亡.结果:Western blot及q RT-PCR法显示,AFAP-1L2蛋白及m R N A表达水平在低分化胰腺癌细胞系中表达水平高于中分化及高分化细胞系.si AFAP-1L2转染后磷脂酰肌醇3激酶a亚单位(P I3K C A)蛋白在s i A FA P-1L2组的表达量明显低于A FA P-1L2组及小干扰RNA(small interfering RNA,si RNA)control组(F=9.280,P=0.0139),a蛋白激酶(a-Akt)在si AFAP-1L2细胞的表达量高于MOCK细胞及si RNA control细胞(F=7.719,P=0.0219),磷酸化a蛋白激酶(a-p A k t)在si AFAP-1L2细胞的表达量低于MOCK细胞及si RNA control细胞(F=5.507,P=0.0439).PI3KCAm RNA在si AFAP-1L2组的表达量明显低于AFAP-1L2组及si RNA control组(F=20.16,P=0.0022),a-Akt m RNA在si AFAP-1L2细胞的表达量高于MOCK细胞及siRNA control细胞(F=6.068,P=0.0362),a-p Akt m R N A在s i A FA P-1L2细胞的表达量低于MOCK细胞及si RNA control细胞(F=10.33,P=0.0114).MTT检测显示,在48、72及96h siAFAP-1L2干扰后Mia PaCa-2细胞增殖能力下降(F=3.924,P<0.05;F=6.812,P<0.01;F=7.003,P<0.01).流式细胞检测显示,si AFAP-1L2干扰后G1期细胞比例增高,G2及S期比例减少(F=4.87,F=5.26,F=4.94,均P<0.05),si AFAP-1L2干扰后Mia Pa Ca-2细胞凋亡率增高(F=7.231,P<0.01).结论:在分化程度低的胰腺癌细胞中AFAP-1L2表达较高,AFAP-1L2通过PI3K/Akt通路影响胰腺癌细胞增殖、细胞周期及凋亡,可作为胰腺癌治疗新的靶向候选基因.展开更多
基金Supported by a grant from the“Ten Thousand Plan”Youth Talent Project in Yunnan Province(no grant number is applicable).
文摘Objective This meta-analysis explored whether the expression of actin filament-associated protein 1 antisense RNA 1(AFAP1-AS1)is related to the prognosis and clinicopathological features of patients with cancer.Methods PubMed,EMBASE,and Cochrane Library were systematically searched.Hazard ratios(HRs)with 95%confidence intervals(CIs)were used to assess the prognostic value based on overall survival(OS),disease-free survival(DFS),and progression-free survival(PFS).Odds ratios(ORs)with 95%CIs were used to determine the relationships between AFAP1-AS1 and clinicopathological features,such as large tumor size(LTS),high tumor stage(HTS),poor histological grade(PHG),lymph node metastasis(LNM),and distant metastasis(DM).Results Thirty-five eligible articles and 3433 cases were analyzed.High AFAP1-AS1 expression,compared to low AFAP1-AS1 expression,correlated with significantly shorter OS(HR=2.15,95%CI=1.97-2.34,P<0.001),DFS(HR=1.37,95%CI=1.19-1.57,P<0.001),and PFS(HR=1.97,95%CI=1.56-2.50,P<0.001)in patients with cancer.In various cancers,elevated AFAP1-AS1 expression was significantly associated with LTS(OR=2.76,95%CI=2.16-3.53,P<0.001),HTS(OR=2.23,95%CI=1.83-2.71,P<0.001),and PHG(OR=1.39,95%CI=1.08-1.79,P=0.01)but not LNM(OR=1.59,95%CI=0.88-2.85,P=0.12)or DM(OR=1.81,95%CI=0.90-3.66,P=0.10).Conclusion High AFAP1-AS1 expression was associated with prognostic and clinicopathological features,suggesting that AFAP1-AS1 is a prognostic biomarker for human cancers.
文摘【目的】观察苦瓜皂苷G对糖尿病肾病(DN)大鼠的治疗作用及机制。【方法】从55只大鼠中随机抽取45只采用一次性腹腔注射链脲佐菌素(STZ)法诱导DN模型。将造模成功的38只大鼠随机分为模型组9只、苦瓜皂苷G低剂量组9只、苦瓜皂苷G中剂量组10只、苦瓜皂苷G高剂量组10只。剩余10只大鼠设为正常组。对应灌胃给药4周后,酶联免疫吸附分析(ELISA)检测大鼠24 h尿蛋白水平,血糖仪检测空腹血糖(FBG),全自动生化分析仪检测血肌酐(SCr)、血尿素氮(BUN)水平,苏木素-伊红染色(HE)法观察大鼠肾组织病理学变化,采用实时定量聚合酶链反应(RT-qPCR)和Western Blot法分别检测E-钙黏蛋白(E-cad)、α-平滑肌肌动蛋白(α-SMA)、Notch1、锌指蛋白转录因子1(Snail1)、Jagged1 m RNA及蛋白表达情况。【结果】与正常组比较,模型组24 h尿蛋白、FBG、血清SCr和BUN水平及肾组织α-SMA、Notch1、Snail1、Jagged1 mRNA及蛋白水平显著升高,肾组织E-cad m RNA及蛋白水平降低,肾组织可见肾小球萎缩、肾小管扩张及间质纤维化;与模型组比较,苦瓜皂苷G低、中、高剂量组24 h尿蛋白、FBG、血清SCr和BUN水平及肾组织α-SMA、Notch1、Snail1、Jagged1 mRNA及蛋白水平显著降低,肾组织E-cad m RNA及蛋白水平升高,肾组织病理损伤得到改善。【结论】苦瓜皂苷G可有效改善DN大鼠肾功能障碍、减轻肾脏纤维化,其机制可能与通过调控Notch/Snail1信号通路,抑制α-SMA和Jagged1 mRNA及蛋白表达,增强E-cad mRNA及蛋白表达有关。
基金supported by a grant from the Shanxi Province Foundation for Returness(2012-4)
文摘BACKGROUND: We previously showed that insulin-like growth factor binding protein-related protein 1 (IGFBPrP1) is a novel mediator in liver fibrosis. Transforming growth factor beta 1 (TGF beta 1) is known as the strongest effector of liver fibrosis. Therefore, we aimed to investigate the detailed interaction between IGFBPrP1 and TGF beta 1 in primary hepatic stellate cells (HSCs). METHODS: We overexpressed TGF beta 1 or IGFBPrP1 and inhibited TGF beta 1 expression in primary HSCs for 6, 12, 24, 48, 72, and 96 hours to investigate their interaction and observe the accompanying expressions of a-smooth muscle actin (alpha-SMA), collagen I, fibronectin, and phosphorylated-mothers against decapentaplegic homolog 2/3 (p-Smad2/3). RESULTS: We found that the adenovirus vector encoding the TGF beta 1 gene (AdTGF beta 1) induced IGFBPrP1 expression while that of alpha-SMA, collagen I, fibronectin, and TGF beta 1 increased gradually. Concomitantly, AdIGFBPrP1 upregulated TGF beta 1, alpha-SMA, collagen I, fibronectin, and p-Smad2/3 in a time-dependent manner while IGFBPrP1 expression was decreased at 96 hours. Inhibition of TGF beta 1 expression reduced the IGFBPrP1-stimulated expression of alpha-SMA, collagen I, fibronectin, and p-Smad2/3. CONCLUSIONS: These findings for the first time suggest the existence of a possible mutually regulation between IGFBPrP1 and TGF beta 1, which likely accelerates liver fibrosis progression. Furthermore, IGFBPrP1 likely participates in liver fibrosis in a TGF beta 1-depedent manner, and may act as an upstream regulatory factor of TGF beta 1 in the Smad pathway.
文摘Cardiotrophin-1 （CT-1） activates a distinct form of cardiac muscle cell hypertrophy in which the sarcomeric units are assembled in series. The aim of the study was to determine the expres- sion pattern of sarcomeric contractile protein α-actin, specialized eytoskeletal protein α-actinin and mitochondrial uncoupling protein-2 （UCP2） in myocardial remodeling induced by chronic exposure to CT-1. Kunming mice were intraperitoneally injected with carboxy-terminal polypeptide （CP） of CT-1 （CT-1-CP, 500 μg·kg-1·day^-1） for 1, 2, 3 and 4 week （s）, respectively （4 groups obtained according to the injection time, n=10 each, with 5 males and 5 females in each group）, Those injected with physiological saline for 4 weeks served as controls （n=10, with 5 males and 5 females）. The heart tissues of mice were harvested at 1, 2, 3 or 4 week （s）. Immunohistochemistry （IHC） and Western blotting （WB） were used to detect the distribution and expression of sarcomeric α-actin, α-aetinin and mitoehondrial UCP2 in myocardial tissues. IHC showed that α-actin was mainly distributed around the nuclei of cardiomyo- cytes, α-actinin concentrated around the striae and UCP2 scattered rather evenly in the plasma. The ex- pression of α-actin was slightly greater than that of α-actinin and UCP2 in the control group （IHC： χ^2=6.125; WB： F=0.249, P〉0.05） and it gradually decreased after exposure to CT-1-CP. There was no significant difference in the expression of α-actin between the control group and the CT-1-CP-treated groups （χ^2=7.386, P〉0.05）. But Western blotting revealed significant difference in the expression of α-actin between the control group and the 4-week CT-1-CP-treated group （F=2.912; q=4.203, P〈0.05）. Moreover, it was found that the expression of α-actinin increased stepwise with the exposure time in CT-1-CP-treated groups and differed significantly between CT-1-CP-treated groups and the control group （ICH： χ^2=21.977; WB： F=50.388; P〈0.01）. The expression of UCP2 was initially increased （WB： control group vs. 1- or 2-week group, q values： 5.603 and 9.995, respectively, P〈0.01） and then de- creased （WB： control group vs. 3-week group, q=4.742, P〈0.01; control group vs. 4-week group, q=0.558, P〉0.05）. It was suggested that long-term exposure to CT-1-CP could lead to the alteration in the expression of sarcomeric α-actin, α-actinin and mitochonclrial UCP2. The different expressions of sarcomeric structure proteins and mitochondrial UCP2 may be involved in myocardial remodeling.
文摘目的:检测肌动蛋白丝相关蛋白1相似蛋白(actin filament-associated protein 1-like 2,A FA P-1L2)在不同分化程度胰腺癌细胞株中的表达,并观察其对下游磷脂酰肌醇3激酶/蛋白激酶(phosphatidylinositol 3 kinase/protein kinase B,PI3K/Akt)通路调控作用及对胰腺癌细胞的增殖、周期及凋亡的影响.方法:以Western blot及实时定量PCR检测(real-time quantitative PCR,q RT-PCR)法对不同分化程度胰腺癌细胞系PANC-1、Mia Pa Ca-2、Colo-357、BXPC-3、SW1990及CFPAC-1中AFAP-1L2表达进行检测;构建靶向AFAP-1L2的干扰质粒si AFAP-1L2,转染Mia Pa Ca-2细胞,以Western blot及q RTPCR法检测AFAP-1L2下调后PI3K/Akt通路蛋白及m RNA变化;四甲基偶氮唑盐微量酶反应比色法(methyl-thiazolyl-tetrazolium,MTT)法检测转染后Mia Pa Ca-2细胞增殖;流式细胞术检测细胞周期及凋亡.结果:Western blot及q RT-PCR法显示,AFAP-1L2蛋白及m R N A表达水平在低分化胰腺癌细胞系中表达水平高于中分化及高分化细胞系.si AFAP-1L2转染后磷脂酰肌醇3激酶a亚单位(P I3K C A)蛋白在s i A FA P-1L2组的表达量明显低于A FA P-1L2组及小干扰RNA(small interfering RNA,si RNA)control组(F=9.280,P=0.0139),a蛋白激酶(a-Akt)在si AFAP-1L2细胞的表达量高于MOCK细胞及si RNA control细胞(F=7.719,P=0.0219),磷酸化a蛋白激酶(a-p A k t)在si AFAP-1L2细胞的表达量低于MOCK细胞及si RNA control细胞(F=5.507,P=0.0439).PI3KCAm RNA在si AFAP-1L2组的表达量明显低于AFAP-1L2组及si RNA control组(F=20.16,P=0.0022),a-Akt m RNA在si AFAP-1L2细胞的表达量高于MOCK细胞及siRNA control细胞(F=6.068,P=0.0362),a-p Akt m R N A在s i A FA P-1L2细胞的表达量低于MOCK细胞及si RNA control细胞(F=10.33,P=0.0114).MTT检测显示,在48、72及96h siAFAP-1L2干扰后Mia PaCa-2细胞增殖能力下降(F=3.924,P<0.05;F=6.812,P<0.01;F=7.003,P<0.01).流式细胞检测显示,si AFAP-1L2干扰后G1期细胞比例增高,G2及S期比例减少(F=4.87,F=5.26,F=4.94,均P<0.05),si AFAP-1L2干扰后Mia Pa Ca-2细胞凋亡率增高(F=7.231,P<0.01).结论:在分化程度低的胰腺癌细胞中AFAP-1L2表达较高,AFAP-1L2通过PI3K/Akt通路影响胰腺癌细胞增殖、细胞周期及凋亡,可作为胰腺癌治疗新的靶向候选基因.
