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猕猴桃(Actinidia Lindl.)叶片与果实维生素C含量的相关性研究 被引量:13
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作者 张蕾 王彦昌 黄宏文 《武汉植物学研究》 CSCD 北大核心 2010年第6期750-755,共6页
以猕猴桃属中华猕猴桃(Actinidia chinensis)32个品种和1个种间杂交后代群体为研究对象,对猕猴桃属植物叶片与果实维生素C含量的相关性进行了研究。结果表明,在中华猕猴桃种内水平上,幼果与成熟果实的果肉维生素C含量间存在极显著的正... 以猕猴桃属中华猕猴桃(Actinidia chinensis)32个品种和1个种间杂交后代群体为研究对象,对猕猴桃属植物叶片与果实维生素C含量的相关性进行了研究。结果表明,在中华猕猴桃种内水平上,幼果与成熟果实的果肉维生素C含量间存在极显著的正相关关系;在种间杂交后代群体中成熟叶片和成熟果实的维生素C含量存在极显著正相关关系,为利用早期相关性状开展猕猴桃育种的可行性提供了理论依据。此外,对15个常见中华猕猴桃品种的果实维生素C含量进行了多重比较,为人工杂交时的亲本选择提供了依据。 展开更多
关键词 猕猴桃 维生素C 相关性
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Genome wide association analysis identifies candidate genes for fruit quality and yield in Actinidia eriantha
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作者 Yingzhen Wang Ying Wu +7 位作者 Xinlei Wang Wangmei Ren Qinyao Chen Sijia Zhang Feng Zhang Yunzhi Lin Junyang Yue Yongsheng Liu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第6期1929-1939,共11页
Quality and yield are the primary concerns in kiwifruit breeding,but research on the genetic mechanisms of fruit size,shape,and ascorbic acid(ASA)content is currently very limited,which restricts the development of ki... Quality and yield are the primary concerns in kiwifruit breeding,but research on the genetic mechanisms of fruit size,shape,and ascorbic acid(ASA)content is currently very limited,which restricts the development of kiwifruit molecular breeding.In this study,we obtained a total of 8.88 million highly reliable single nucleotide polymorphism(SNP)markers from 140 individuals from the natural hybrid offspring of Actinidia eriantha cv.‘White’using whole genome resequencing technology.A genome-wide association study was conducted on eight key agronomic traits,including single fruit weight,fruit shape,ASA content,and the number of inflorescences per branch.A total of 59 genetic loci containing potential functional genes were located,and candidate genes related to single fruit weight,fruit length,ASA content,number of inflorescences per branch and other traits were identified within the candidate interval,such as AeWUSCHEL,AeCDK1(cell cycle dependent kinase),AeAO1(ascorbic oxidase)and AeCO1(CONSTANS-like 4).After constructing an RNAi vector for AeAO1 and injecting it into the fruit of cv.‘Midao 31’to interfere with the expression of the AeAO1 gene,the results showed that the activity of ascorbic oxidase in the fruit of‘Midao 31’significantly decreased,while the content of ASA significantly increased.This study provides valuable insights into the genetic basis of variation in A.eriantha fruit traits,which may benefit molecular marker-assisted breeding efforts. 展开更多
关键词 actinidia eriantha GWAS SNP QTLS fruit quality
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INTERSPECIFIC SOMATIC HYBRIDS IN ACTINIDIA 被引量:17
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作者 肖尊安 韩碧文 《Acta Botanica Sinica》 CSCD 1997年第12期1110-1117,共8页
Protoplasts isolated from cotyledon-derived calli of Actinidia chinensis var. chinensis (2n = 2x=58) were fused by the PEG method with cotyledon-callus protoplasts of A. deliciosa var. deliciosa (2n = 6x = 174) or wit... Protoplasts isolated from cotyledon-derived calli of Actinidia chinensis var. chinensis (2n = 2x=58) were fused by the PEG method with cotyledon-callus protoplasts of A. deliciosa var. deliciosa (2n = 6x = 174) or with mesophyll protoplasts of A. kolomikta (2n = 2x = 58), respectively. Randomly amplified polymorphic DNA (RAPD) markers and flow cytometry was used to confirm the occurrence of somatic hybrids. RAPD results with some primers surveyed indicated that one clone (A. chinensis + A. deliciosa) and four clones (A. chinensis + A. kolomikta) had RAPD banding patterns which combined the parental banding profiles. Ploidy levels of the (A. chinensis + A. deliciosa) clone were deduced as octoploid (2n = 8x), and the (A. chinensis + A. kolomikta) clones were tetraploid (2n = 4x), triploid (2n = 3x) or pentaploid (2n = 5x). The clones were confirmed as interspecific somatic hybrids in Actinidia. 展开更多
关键词 actinidia chinensis actinidia deliciosa actinidia kolomikta interspecific somatic hybrid
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Dynamic Variation in Sugar,Acid,and ASA Contents of ‘Ganmi 6' Kiwifruit(Actinidia eriantha Benth) Fruits 被引量:3
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作者 陈楚佳 陶俊杰 +2 位作者 曲雪艳 黄春辉 徐小彪 《Agricultural Science & Technology》 CAS 2015年第12期2589-2591,2595,共4页
[Objective] The variation of sugar, acid and AsA contents in fruits of ‘Ganmi 6' kiwifruit (Actinidia eriantha Benth) were investigated during fruit develop- ment. [Method] Kiwi fruits were randomly taken as mater... [Objective] The variation of sugar, acid and AsA contents in fruits of ‘Ganmi 6' kiwifruit (Actinidia eriantha Benth) were investigated during fruit develop- ment. [Method] Kiwi fruits were randomly taken as materials every 15 days since 20 days after full bloom (DAFB) to 170 DAFB until in mature stage. [Result] The results showed that during fruit development of ‘Ganmi 6', the total sugar soluble contents had a rising trend with relatively stable at 95 DAFB, then rose until har- vest with the maximum content (10.35%). The titratable acid content showed a trend of increasing, then declining, then increase to the harvest content (1.10%). From the sugar acid ratio, we can knew it decreased in 95 DAFB, then up to the max (9.38). The changes of AsA contents showed double ‘S' shape, decreased af- ter the first increased rapidly, slightly increased and then decreased in the early harvest. [Conclusion] It provided a theoretical basis for scientific cultivation methods to explore the nutrients regulation. 展开更多
关键词 actinidia eriantha Benth KIWIFRUIT SUGAR ACID ASA
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猕猴桃属(Actinidia)植物的离体种质保存 被引量:6
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作者 陈维伦 郭东红 +1 位作者 安和祥 朱至清 《生物多样性》 CAS CSCD 1998年第4期278-281,共4页
本文研究了猕猴桃属植物离体种质资源保存的方法。在一年中于盛夏时采取的植物材料在离体培养时能得到最好的效果;改进的剥离茎尖的方法使污染率大大降低,在MS附加BA0.5,Z0.1,GA0.1~0.5mg/L,蔗糖3%,琼... 本文研究了猕猴桃属植物离体种质资源保存的方法。在一年中于盛夏时采取的植物材料在离体培养时能得到最好的效果;改进的剥离茎尖的方法使污染率大大降低,在MS附加BA0.5,Z0.1,GA0.1~0.5mg/L,蔗糖3%,琼脂0.55%的培养基中茎尖生长良好并且不产生不定芽。通过茎尖培养方法已保存了10多个种,40多个猕猴桃的离体种质。 展开更多
关键词 离体 种质保存 猕猴桃
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Physiological Responses of Actinidia arguta (Seib.et.Zucc.) to Low Temperature Stress 被引量:4
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作者 马丽 郭廷松 +5 位作者 陈学森 臧得奎 公小君 孙晶晶 邱艳芬 王延玲 《Agricultural Science & Technology》 CAS 2017年第5期767-770,776,共5页
[Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta (Seib.et.Zucc.), and provide a theoretical basis for the introduc- tion and breeding of cold resistant varieties. [Meth... [Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta (Seib.et.Zucc.), and provide a theoretical basis for the introduc- tion and breeding of cold resistant varieties. [Method] A. arguta as the test material was processed at room temperature of 4 ℃ to determine the contents of chloro- phyll, water soluble sugar, water soluble protein, free proline, MDA, and antioxidant enzymes (POD activity, SOD activity, CAT activity) with the treatment at 25℃ as the control. [Results] The results indicated that with the prolonging of stress time, the content of chlorophyll was first increased and then decreased, and the content of MDA was increased; soluble sugar content of seedling leaf decreased gradually, while the soluble protein and proline content of seedling leaves were first increased and then decreased; the activities of SOD and POD were displayed a decline trend, meanwhile the activities of CAT was first increased and then decreased. [Conclu- sion] The various indicators showed that under low temperature stress, the chloro- phyll content decreased accompanied with membrane lipid peroxidation and MDA accumulation, and soluble sugar, soluble protein, free proline and antioxidant en- zymes play a protective role in different degrees. 展开更多
关键词 actinidia arguta (Seib.et.Zucc.) Low temperature stress Physiologicalresponse
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猕猴桃属(Actinidia)植物的遗传多样性 被引量:182
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作者 黄宏文 龚俊杰 +3 位作者 王圣梅 何子灿 张忠慧 李建强 《生物多样性》 CAS CSCD 2000年第1期1-12,共12页
猕猴桃属(Actinidia)植物全世界有66个种、约118个种下分类单位(变种、变型)。中国有猕猴桃属62个种,猕猴桃遗传资源极为丰富。本文就猕猴桃的遗传资源多样性主要特点作概要综述:1)形态性状多样性(重要的园艺及经济性状);... 猕猴桃属(Actinidia)植物全世界有66个种、约118个种下分类单位(变种、变型)。中国有猕猴桃属62个种,猕猴桃遗传资源极为丰富。本文就猕猴桃的遗传资源多样性主要特点作概要综述:1)形态性状多样性(重要的园艺及经济性状);2)营养成分及风味多样性;3)性别变异;4)染色体倍性变异;5)同工酶水平遗传多样性;6)DNA水平遗传多样性。 展开更多
关键词 遗传多样性 形态性状 营养成分 猕猴桃属植物
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江西猕猴桃属(Actinidia)植物的分布及其区系特征 被引量:6
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作者 姚小洪 徐小彪 +2 位作者 高浦新 李作洲 黄宏文 《武汉植物学研究》 CSCD 北大核心 2005年第3期257-261,共5页
通过野外调查和查阅大量标本、文献,对江西猕猴桃属植物的分布和区系特征进行了系统研究。江西省境内分布有猕猴桃属植物20种和11变种(或变型),其中葛枣猕猴桃(Actinidiapolygama(Sieb.etZucc.)Maxim.)、灰毛猕猴桃(A.cinerascensC.F.Li... 通过野外调查和查阅大量标本、文献,对江西猕猴桃属植物的分布和区系特征进行了系统研究。江西省境内分布有猕猴桃属植物20种和11变种(或变型),其中葛枣猕猴桃(Actinidiapolygama(Sieb.etZucc.)Maxim.)、灰毛猕猴桃(A.cinerascensC.F.Liang)、楔叶猕猴桃(A.fasciculoidesvar.cuneataC.F.Liang)和簇花猕猴桃(A.fasciculoidesC.F.Liang)为江西新分布。江西猕猴桃属植物区系特征表现在:(1)种类丰富;(2)特有现象较明显;(3)多型性突出;(4)地理成分复杂,以中国特有分布式样为主;(5)种间、种内分化较强烈;(6)与邻近地区猕猴桃属植物的关系密切。还对江西猕猴桃属植物的分布与自然生境的关系进行了探讨。 展开更多
关键词 猕猴桃属 地理分布 区系特征 江西
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Determination of Heavy Metals in Dendrobium candidium Wall.ex Lindl.by ICP-MS 被引量:3
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作者 郑龙 杨春亮 +1 位作者 苏子鹏 叶剑芝 《Agricultural Science & Technology》 CAS 2015年第12期2592-2595,共4页
[Objective] This research aimed to establish an inductively coupled plasma mass spectrometry (ICP-MS) method for determination of five kinds of heavy metals (Pb, Cd, Cu, As and Hg) in Dendrobium candidium Wall. ex... [Objective] This research aimed to establish an inductively coupled plasma mass spectrometry (ICP-MS) method for determination of five kinds of heavy metals (Pb, Cd, Cu, As and Hg) in Dendrobium candidium Wall. ex Lindl. [Method] The samples were digested in HNO3-H202 acids system by closed vessel microwave di- gestion. At the same time, internal standard was added to avoid the matrix effect. [Result] The five kinds of heavy meals all showed good linear relationships, and the correlation coefficients ranged from 0.998 2 to 0.999 9. The detection limits of the five kinds of heavy metals were in the range of 0.008-0.750 μg/L, while the stan- dard recovery rates were in the range of 90.4%-96.9%. The relative standard devi- ations ranged from 1.6% to 8.2%. The heavy metals in the Ginseng and Astragalus national standard samples were determined by the established ICP-MS method, and the results were in conformity with the standard values. [Conclusion] The established method has simple and convenient operation and accurate and reliable results, and it meets the requirements by determination of heavy metals in Dendrobium candididium Wall. ex Lindl. 展开更多
关键词 Dendrobium candidium Wall. ex lindl. Heavy metals Microwave diges- tion: ICP-MS
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陕西省猕猴桃细菌性溃疡病菌(Pseudomonas syringae pv.actinidiae)Rep-PCR的遗传多样性分析 被引量:12
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作者 高小宁 郑州 +2 位作者 赵志博 秦虎强 黄丽丽 《果树学报》 CAS CSCD 北大核心 2016年第3期340-349,共10页
【目的】由丁香假单胞猕猴桃致病变种(Pseudomonas syringae pv.actinidiae,Psa)引起的溃疡病是猕猴桃生产中最具毁灭性的病害,明确病菌的群体遗传特性将对了解病害发生规律和防治策略的制定具有重要理论意义。【方法】采用ERIC-PCR(ent... 【目的】由丁香假单胞猕猴桃致病变种(Pseudomonas syringae pv.actinidiae,Psa)引起的溃疡病是猕猴桃生产中最具毁灭性的病害,明确病菌的群体遗传特性将对了解病害发生规律和防治策略的制定具有重要理论意义。【方法】采用ERIC-PCR(enterobacterial repetitive inter-genic consensus)和BOX-PCR指纹技术分析了陕西省猕猴桃主要栽培区Psa的遗传特性。【结果】ERIC-PCR聚类结果显示,相似系数为0.626时,72个Psa菌株被分为6个类群,其中供试菌株中的75.7%属于第Ⅱ类群,且菌株无明显的采集地和寄主品种的聚类。BOX-PCR指纹聚类分析可将86个Psa菌株分为8个类群(相似系数为0.668),其中第Ⅰ类群菌株数量最多,占供试菌株数量的69.8%,其地理来源为眉县、周至、杨凌及其全部的意大利供试菌株,其中来源眉县的81.6%的菌株都聚集在该类群;菌株未表现出明显的寄主品种类群。【结论】陕西省猕猴桃溃疡病菌基因组存在丰富的遗传多样性;ERIC-PCR和BOX-PCR多态性分析技术可为我国Psa基因多样性的研究提供一个有效途径。 展开更多
关键词 猕猴桃细菌性溃疡病菌 ERIC-PCR BOX-PCR 遗传多样性
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大籽猕猴桃(Actinidia macrosperma)离体再生系统的建立 被引量:18
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作者 姜维梅 李凤玉 《浙江大学学报(农业与生命科学版)》 CAS CSCD 北大核心 2003年第3期295-299,共5页
大籽猕猴桃,其根茎是著名抗癌中药,中药名称猫人参,其野生资源已近枯竭.本文选用大籽猕猴桃的茎段(至少带一个节)和叶片作为外植体,筛选出了从启动、快繁直至生根等各个阶段的最适培养基,在实验室建立了程序简单、重复性好的离体快繁体... 大籽猕猴桃,其根茎是著名抗癌中药,中药名称猫人参,其野生资源已近枯竭.本文选用大籽猕猴桃的茎段(至少带一个节)和叶片作为外植体,筛选出了从启动、快繁直至生根等各个阶段的最适培养基,在实验室建立了程序简单、重复性好的离体快繁体系,实现了大籽猕猴桃组培苗的快速繁殖.实验发现以茎段作为外植体效果较好,既容易消毒,又能很快诱导腋芽萌发;以叶片作为外植体,能成功诱导出愈伤组织,但此愈伤组织的分化能力极低.以茎段作为外植体获得的无菌培养物在不同发育阶段适宜的培养基组成:外植体启动培养基为MS+BA5μmol/L+NAA1μmol/L;最适增殖培养基为MS+BA2μmol/L+GA1.5μmol/L+ZT4μmol/L,35d繁殖系数为9~10;最适生根培养基为1/4MS+IBA2μmol/L,生根率可达100%,培养35d,平均苗高6.28cm,平均移栽成活率为91%. 展开更多
关键词 大籽猕猴桃 组织培养 离体培养 植抹再生系统 快速繁殖 外植体 培养基 药用植物
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云南木瓜属(Chaenomeles Lindl.)植物的民族植物学初步研究 被引量:8
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作者 高富 裴盛基 +2 位作者 杨立新 闵康 孙杉 《安徽农业科学》 CAS 北大核心 2011年第7期3950-3954,共5页
蔷薇科(Rosaceae)木瓜属(Chaenomeles)植物共有5种,我国全产。该属为重要观赏植物和果品,世界各地均有栽培。木瓜在我国栽培历史悠久,是重要的中药材品种。云南木瓜属植物4种,种下品种较多,大多品质优良,具有较高的药用、营养保健开发... 蔷薇科(Rosaceae)木瓜属(Chaenomeles)植物共有5种,我国全产。该属为重要观赏植物和果品,世界各地均有栽培。木瓜在我国栽培历史悠久,是重要的中药材品种。云南木瓜属植物4种,种下品种较多,大多品质优良,具有较高的药用、营养保健开发潜力。云南民族民间利用植物于医疗保健、生态防护和增加经济收入方面均十分看好木瓜资源的潜力;但与其他发达地区相比,在品种培育、产业开发方面仍然有一定的差距。进一步加快调查、整理、发掘云南民族民间开发利用木瓜资源的传统知识体系,与现代化学分析手段相结合,发挥木瓜传统栽培模式的生态和经济效益,促进云南木瓜资源综合开发利用技术进步,将会在农业结构调整中,为云南山区农业生态环境改善、农民增收、民族地区繁荣与和谐发展做出贡献。 展开更多
关键词 木瓜属 植物资源利用 传统知识 民族植物学
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中华猕猴桃(Actinidiachinensis)在中国的适生性及其潜在地理分布模拟预测 被引量:13
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作者 张杰 敖子强 +2 位作者 吴永明 杨春燕 李敏 《热带地理》 2017年第2期218-225,共8页
利用181个地理分布调查数据,基于Arc GIS对中华猕猴桃的目前分布进行空间重建,利用Max Ent生态位模型对中华猕猴桃在中国的适生性及其未来气候变化下潜在可扩散地理范围进行了模拟与预测,分析中华猕猴桃在中国的适生性和扩散潜力及其空... 利用181个地理分布调查数据,基于Arc GIS对中华猕猴桃的目前分布进行空间重建,利用Max Ent生态位模型对中华猕猴桃在中国的适生性及其未来气候变化下潜在可扩散地理范围进行了模拟与预测,分析中华猕猴桃在中国的适生性和扩散潜力及其空间格局,在未来气候变化背景下,预测中华猕猴桃当代及未来至2050年适生区分布格局变化。结果表明:中华猕猴桃当前的潜在适生区主要分布在23°―35°N之间和102°E以东的中亚热带山地丘陵区,如秦岭―大巴山、云贵高原、罗霄山脉―南岭和武夷山脉等地区。中华猕猴桃在当前气候条件下适生区总面积为2 382 381 km2,占中国版图的25.19%,在中等温室气体排放(RCP4.5)情景下预测至2050年,适生区总面积相对当前气候情景略有减少,但空间分布格局变化强烈。预测结果表明:在未来气候变暖影响下,中华猕猴桃潜在适生区具有向北或高纬度地区迁移的趋势。 展开更多
关键词 中华猕猴桃 潜在分布 适生性 MaxEnt模型 气候变化
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中华猕猴桃(Actinidia Chinensis)雌雄异株性状变异的研究 被引量:6
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作者 朱道圩 张俊华 《河南农业大学学报》 CAS CSCD 1995年第1期1-5,共5页
通过2a调查,发现了介于中华猕猴桃雌株与雄株之间的中间类型,但该类型数量稀少,在野生群体中只占0.5%左右。雌雄异株性状的变异可以从雌株、雄株以及中间类型的花器发育水平上体现。雌花的雌蕊可育,但花粉没有生活力;雄花雌... 通过2a调查,发现了介于中华猕猴桃雌株与雄株之间的中间类型,但该类型数量稀少,在野生群体中只占0.5%左右。雌雄异株性状的变异可以从雌株、雄株以及中间类型的花器发育水平上体现。雌花的雌蕊可育,但花粉没有生活力;雄花雌蕊败育,但花粉正常;而中间类型花朵的雌蕊部分发育,而且花粉也有生活力。仅在雌株与中间类型上可以见到明显的“柱头一花柱”结构,但二者在柱头表面乳突的密度与发育水平上显著不同。中间类型花朵的柱头上乳突稀少,而且部分萎陷;花粉粒能在其柱头上发芽,但大多数花粉管不能伸入柱头组织。中间类型的发现不仅对研究雌雄异株性状的演化有一定意义,而且也为培育中华猕猴桃雌雄同株新品种奠定了基础。 展开更多
关键词 中华猕猴桃 雌雄异株 性状变异 猕猴桃
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黄刺玫(Rosa xanthina Lindl.)嫩枝扦插生根过程的解剖学研究 被引量:6
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作者 孙婷婷 胡宝忠 +1 位作者 陈玉霞 付洪冰 《东北农业大学学报》 CAS CSCD 2008年第7期53-56,共4页
文章对黄刺玫嫩枝扦插生根过程中不定根的发生发育进行了解剖学研究,结果表明,黄刺玫插条内未见潜伏根原基,属诱生根原基生根型。黄刺玫的诱生根原基产生于愈伤组织,为较难生根树种。
关键词 黄刺玫 嫩枝扦插 解剖学 不定根
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猕猴桃细菌溃疡病(Actinidia bacterial canker)病原细菌鉴定 被引量:47
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作者 王忠肃 唐显富 刘绍基 《西南农业大学学报(自然科学版)》 CSCD 1992年第6期500-503,共4页
对四川省苍溪县猕猴桃的细菌病害进行系统鉴定表明,该病属细菌溃疡病,其病原细菌为 Pseudomonas syringae Pv.actinidea。病原细菌的鉴定属国内首次报导.
关键词 猕猴桃 溃疡病 病原 细菌 鉴定
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猕猴桃属植物狗枣子(Actinidia kolomikta(Maxim.&Rupr.)Maxim.)的生态地理分布及其植物地理学意义 被引量:6
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作者 邓德山 《植物研究》 CAS CSCD 北大核心 2002年第2期163-167,共5页
作者认为狗枣子 (Actinidiakolomikta (Maxim .&Rupr.)Maxim .)是一个多型的、具有两个间断布区的适应于地带性针阔叶混交林生境的猕猴桃属植物。文中还根据狗枣子的生态地理分布特征 ,认为狗枣子的现代中国西南 - -东北亚间断分布... 作者认为狗枣子 (Actinidiakolomikta (Maxim .&Rupr.)Maxim .)是一个多型的、具有两个间断布区的适应于地带性针阔叶混交林生境的猕猴桃属植物。文中还根据狗枣子的生态地理分布特征 ,认为狗枣子的现代中国西南 - -东北亚间断分布的格局是东亚第三纪以来气候回旋变化的结果所造成的。狗枣子原始分布区 (中国西南高山 )受气候回旋变化的胁迫衍生出次生的东北亚分布区。由于气候的振荡变化 ,水平方向上原有的连续分布区逐渐断裂而形成现代的间断分布格局。据此文中还指出 。 展开更多
关键词 猕猴桃属植物 狗枣子 生态地理分布 植物地理学
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赣中、北地区中华猕猴桃(Actinidia chinensis Planch.)病害调查与鉴定 被引量:5
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作者 何念杰 唐祥宁 朱福荣 《江西农业大学学报》 CAS CSCD 1989年第1期63-67,共5页
经初步调查鉴定我省赣中、北地区中华猕猴桃(Actinidia chinensis Planch.)的病害有13种。其中真菌性病害11种,线虫病害、生理性病害各1种.
关键词 赣中北 中华猕猴桃 病害
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Effects of Lanthanum and Europium on Rooting of Plantlet Eriobotrya Japonica Lindl. in vitro 被引量:5
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作者 宋卫平 洪法水 +1 位作者 万志刚 周玉珍 《Journal of Rare Earths》 SCIE EI CAS CSCD 2002年第6期658-662,共5页
The effect of La 3+ and Eu 3+ on the rooting of Eriobotrya japonica Lindl. plantlet in vitro was studied with adding La 3+ and Eu 3+ to the rooted medium. The rooting rate, the number of root and the lengt... The effect of La 3+ and Eu 3+ on the rooting of Eriobotrya japonica Lindl. plantlet in vitro was studied with adding La 3+ and Eu 3+ to the rooted medium. The rooting rate, the number of root and the length of root were studied after transplanting 20 d. The activity of peroxidase, nitrate reductase and fresh weight of roots were determined after transplanting 44 d. The results show that the optimum concentration range of La 3+ (1.0~3.0 μmol·L -1), Eu 3+ (2.0~3.0 μmol·L -1) in the rooted medium can increase the rooting rate and the fresh weight of roots, and promote the length of root and raise the activities of peroxidase and nitrate reductase significantly. La 3+ has more effect in improving the rooting rate, root length and the activities of peroxidase and less effect in promoting root fresh weight and the activities of nitrate reductase than Eu 3+. 展开更多
关键词 rare earths LANTHANUM EUROPIUM Eriobotrya japonica lindl plantlet in vitro ROOTING
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Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta(25–35)-induced neuronal injury 被引量:10
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作者 Wei Zhang Qin Wu +3 位作者 Yan-liu Lu Qi-hai Gong Feng Zhang Jing-shan Shi 《Neural Regeneration Research》 SCIE CAS CSCD 2017年第7期1131-1136,共6页
Dendrobium nobile Lindl.alkaloids(DNLA),the active ingredients of a traditional Chinese medicine Dendrobium,have been shown to have anti-oxidative effects,anti-inflammatory action,and protective effect on neurons ag... Dendrobium nobile Lindl.alkaloids(DNLA),the active ingredients of a traditional Chinese medicine Dendrobium,have been shown to have anti-oxidative effects,anti-inflammatory action,and protective effect on neurons against oxygen-glucose deprivation.However,it is not clear whether DNLA reduces amyloid-beta(Aβ)-induced neuronal injury.In this study,cortical neurons were treated with DNLA at different concentrations(0.025,0.25,and 2.5 mg/L)for 24 hours,followed by administration of Aβ(25-35)(10μM).Aβ(25-35) treatments increased cell injury as determined by the leakage of lactate dehydrogenase,which was accompanied by chromatin condensation and mitochondrial tumefaction.The damage caused by Aβ(25-35) on these cellular properties was markedly attenuated when cells were pretreated with DNLA.Treatment with Aβ(25-35)down-regulated the expressions of postsynaptic density-95 mRNA and decreased the protein expression of synaptophysin and postsynaptic density-95,all changes were significantly reduced by pretreatment of cells with DNLA.These findings suggest that DNLA reduces the cytotoxicity induced by Aβ(25-35) in rat primary cultured neurons.The protective mechanism that DNLA confers on the synaptic integrity of cultured neurons might be mediated,at least in part,through the upregulation of neurogenesis related proteins synaptophysin and postsynaptic density-95. 展开更多
关键词 nerve regeneration Dendrobium nobile lindl. alkaloids amyloid beta NEURONS SYNAPSE SYNAPTOPHYSIN postsynaptic density-95 cognitive impairment NEUROPROTECTION neural regeneration
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