Objective：To extract the active component from the root of Actinidia valvata Dunn and to investigate the effects on hepatocellular carcinoma（HCC） cells in vitro.Methods：Total saponin was extracted from the root of...Objective：To extract the active component from the root of Actinidia valvata Dunn and to investigate the effects on hepatocellular carcinoma（HCC） cells in vitro.Methods：Total saponin was extracted from the root of A.valvata（TSAVD）.HCC cells,such as BEL-7402,HepG2,PLC,SMMC-7721,MHCC-97-H, and MHCC-97-L,were treated with TSAVD in 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenytetrazolium bromide（MTT） assay.BEL-7402 and MHCC-97-H cells were also treated respectively with TSAVD at different concentrations for 24 h in wound healing and adhesion assays,and the effects of TSAVD on BEL-7402 and MHCC-97-H cells mobility and adhesion abilities were observed.Meanwhile,the effects of TSAVD on invasion and migration of BEL-7402 and MHCC-97-H cells were also investigated by transwell chamber in invasion and migration assays. Results：TSAVD at 1.5 mg/mL inhibited BEL-7402 cell proliferation with inhibition ratios（IRs） of 61.08%,74.12%, 84.55%at 24,48,and 72 h,respectively.Meanwhile,TSAVD inhibited MHCC-97-H proliferation in a concentrationdependent manner from 1.5 to 0.5 mg/mL,with the IR of 36%at 1.5 mg/mL at 24 h.For SMMC-7721,PLC, and HepG2,the IR was lower than 30%at 1.5 mg/mL at 24 h.In the wound healing assay,mobility abilities of BEL-7402 and MHCC-97-H cells in TSAVD treated groups were significantly weaker than those of the control group.After pretreatment for 24 h with TSAVD,adhesion abilities were reduced in both MHCC-97-H and BEL-7402 cells,with IRs of 48.50%±4.86%and 49.85%±5.25%at 200 ｜xg/mL.The IRs of MHCC-97-H and BEL-7402 cells in the migration assay were 49.13%±2.91%and 79.37%±0.09%at 200μg/mL In the invasion assay,IRs were 69.78%±4.88%and 82.48%±0.25%at 200μg/mL Conclusions：Of all HCC cells,the highest inhibition by TSAVD was seen for BEL-7402 proliferation.TSAVD could restrain adhesion,invasion,mobility,and migration abilities of BEL-7402 and MHCC-97-H cells in vitro.展开更多
Flavonoids are a large group of phenolic secondary metabolites havinga wide range of biochemical and pharmacological effects.Quantitative analysis of flavonoid profiles in the genus Actinidia,which has not been intens...Flavonoids are a large group of phenolic secondary metabolites havinga wide range of biochemical and pharmacological effects.Quantitative analysis of flavonoid profiles in the genus Actinidia,which has not been intensively conducted,is useful to a better understanding of the pattern and distribution of flavonoids.In the present work,a liquid chromatography-electrospray ionization-tandem mass spectrometry(LC-ESI-MS/MS)method was developed to profile the flavonoids,which was then used to determine the dynamic change of 17 biologically active flavonoids in the leaves of Actinidia valvata at the main growing stages,including glucuronides and acylated di-and triglycosides of flavonoids.The contents of flavonoid triglycosides were significantly higher than other flavonoids.The highest concentrations of kaemperol glycosides were observed in June,while other flavonoids showed highest concentrations in October.On the other hand,the contents of four isorhamnetin glycosides were increased sharply in September to October.The flavonoid profiles seem to be related to temperature,UV-B,and water deficit.Further studies are required to examine the functions of flavonoids in the Actinidia valvata and the underlying molecular mechanisms of actions.展开更多
基金Suppo rted bv the National Natural Science Foundation of China(No C0305020)the Traditional Chinese Medicine Modernization Project of Science and Technology Commission of Shanghai Municipality(No.04DZ19808)the Open Project Foundation of Key Laboratory of Liver and Kidney Diseases(Shanghai University of Traditional Chinese Medicine),Ministry of Education
文摘Objective：To extract the active component from the root of Actinidia valvata Dunn and to investigate the effects on hepatocellular carcinoma（HCC） cells in vitro.Methods：Total saponin was extracted from the root of A.valvata（TSAVD）.HCC cells,such as BEL-7402,HepG2,PLC,SMMC-7721,MHCC-97-H, and MHCC-97-L,were treated with TSAVD in 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenytetrazolium bromide（MTT） assay.BEL-7402 and MHCC-97-H cells were also treated respectively with TSAVD at different concentrations for 24 h in wound healing and adhesion assays,and the effects of TSAVD on BEL-7402 and MHCC-97-H cells mobility and adhesion abilities were observed.Meanwhile,the effects of TSAVD on invasion and migration of BEL-7402 and MHCC-97-H cells were also investigated by transwell chamber in invasion and migration assays. Results：TSAVD at 1.5 mg/mL inhibited BEL-7402 cell proliferation with inhibition ratios（IRs） of 61.08%,74.12%, 84.55%at 24,48,and 72 h,respectively.Meanwhile,TSAVD inhibited MHCC-97-H proliferation in a concentrationdependent manner from 1.5 to 0.5 mg/mL,with the IR of 36%at 1.5 mg/mL at 24 h.For SMMC-7721,PLC, and HepG2,the IR was lower than 30%at 1.5 mg/mL at 24 h.In the wound healing assay,mobility abilities of BEL-7402 and MHCC-97-H cells in TSAVD treated groups were significantly weaker than those of the control group.After pretreatment for 24 h with TSAVD,adhesion abilities were reduced in both MHCC-97-H and BEL-7402 cells,with IRs of 48.50%±4.86%and 49.85%±5.25%at 200 ｜xg/mL.The IRs of MHCC-97-H and BEL-7402 cells in the migration assay were 49.13%±2.91%and 79.37%±0.09%at 200μg/mL In the invasion assay,IRs were 69.78%±4.88%and 82.48%±0.25%at 200μg/mL Conclusions：Of all HCC cells,the highest inhibition by TSAVD was seen for BEL-7402 proliferation.TSAVD could restrain adhesion,invasion,mobility,and migration abilities of BEL-7402 and MHCC-97-H cells in vitro.
基金financially supported by National Natural Science Foundation of China(Nos.81102336 and U1203104)Special Project of Biological Medicine of Science and Technology Commission of Shanghai Municipality(No.10431900500)
文摘Flavonoids are a large group of phenolic secondary metabolites havinga wide range of biochemical and pharmacological effects.Quantitative analysis of flavonoid profiles in the genus Actinidia,which has not been intensively conducted,is useful to a better understanding of the pattern and distribution of flavonoids.In the present work,a liquid chromatography-electrospray ionization-tandem mass spectrometry(LC-ESI-MS/MS)method was developed to profile the flavonoids,which was then used to determine the dynamic change of 17 biologically active flavonoids in the leaves of Actinidia valvata at the main growing stages,including glucuronides and acylated di-and triglycosides of flavonoids.The contents of flavonoid triglycosides were significantly higher than other flavonoids.The highest concentrations of kaemperol glycosides were observed in June,while other flavonoids showed highest concentrations in October.On the other hand,the contents of four isorhamnetin glycosides were increased sharply in September to October.The flavonoid profiles seem to be related to temperature,UV-B,and water deficit.Further studies are required to examine the functions of flavonoids in the Actinidia valvata and the underlying molecular mechanisms of actions.
