The study aimed at introducing the design scheme of the active tag in 2.4 GHz frequency band for pig based on RFID technology. And through the actual circuit board test, the following results were come up： communicat...The study aimed at introducing the design scheme of the active tag in 2.4 GHz frequency band for pig based on RFID technology. And through the actual circuit board test, the following results were come up： communication distance and signal strength gradually increased with the data transfer rate reducing in multiples; better communication effects were achieved in the data transfer rate of 60 kbps or 10 kbps; the active tags could be recognized accurately within 50 m, greatly improving the security of swine food safety traceability system.展开更多
About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these mutants...About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these mutants were single copy integration and the average T-DNA insertion number was 2.28. By extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. Histochemical GUS assay with 4,310 primary mutants revealed that the GUS-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. The T-DNA flanking sequences of some mutants were isolated and the T-DNA insertion sites were mapped to the rice genome. The flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. Compared with Arabidopsis and poplar, it is much varied in the T-DNA border junctions in rice.展开更多
文摘The study aimed at introducing the design scheme of the active tag in 2.4 GHz frequency band for pig based on RFID technology. And through the actual circuit board test, the following results were come up： communication distance and signal strength gradually increased with the data transfer rate reducing in multiples; better communication effects were achieved in the data transfer rate of 60 kbps or 10 kbps; the active tags could be recognized accurately within 50 m, greatly improving the security of swine food safety traceability system.
基金supported by the National High Technology Research and Development Program of China (No.2002AAZ2001)the National Natural Sciences Foundation of China (No.30270758 and 30621001)
文摘About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these mutants were single copy integration and the average T-DNA insertion number was 2.28. By extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. Histochemical GUS assay with 4,310 primary mutants revealed that the GUS-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. The T-DNA flanking sequences of some mutants were isolated and the T-DNA insertion sites were mapped to the rice genome. The flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. Compared with Arabidopsis and poplar, it is much varied in the T-DNA border junctions in rice.
