Objective： To investigate the electrical signals propagated along Foot Taiyang Bladder Meridian （BL） in a rat model. Methods： The experiments were performed on Dark-Agouti （DA）, DA.lU and Sprague Dawley （SD） r...Objective： To investigate the electrical signals propagated along Foot Taiyang Bladder Meridian （BL） in a rat model. Methods： The experiments were performed on Dark-Agouti （DA）, DA.lU and Sprague Dawley （SD） rats. The antidromic electrical stimulation was applied on the nerve innervating ＂Pishu＂ （BL 20） to mimic the acupoint electro-acupuncture （EA）. The activities recording from adjacent nerve innervating acupoint ＂Danshu＂ （BL 19） or ＂Weishu＂ （BL 21） were recorded as indics for acupoint, including the mechanical threshold and discharge rate. Results： After mimic EA on BL 20, C and A $ units from adjacent BL 19 or BL 21 were sensitized including the decrease in mechanical threshold and increase in discharge rates in DA, DA.1U and SD rats, especially in DA rats. The average discharge rate increased from 2.40± 0.26 to 6.06± 0.55 and from 1.92±0.42 to 6.17± 1.10 impulse/min （P〈0.01）, and the mechanical threshold decreased from 0.52 ± 0.12 to 0.24 ± 0.05 and from 0.27±0.02 to 0.16±0.01 mmol/L （P〈0.01） in C （n=15） and Aδ （n=18） units in DA rats. The net change in discharge rates from C units were 152.5%, 144.7% and 42.4% in DA, DA.1U and SD rats, respectively, among which DA rat＇s was the highest （P〈0.05）. In A δ units, the net change in DA rats were also the highest （221.5%, 139.2% and 49.2% in DA, DA.lU and SD rats）. Conclusions： These results showed that mimic acupoint EA activated adjacent acupoints along BL in three rat strains, which might be related to propagated sensation along meridians （PSM）. In addition, DA rats were more sensitive and might be a good model animal for PSM research.展开更多
目的:探讨股骨头坏死(osteonecrosis of the femoral head,ONFH)患者的疼痛特点及疼痛区域经脉循行特点。方法:选择2020年5月至2021年7月在广州中医药大学第三附属医院门诊或住院治疗的ONFH患者为研究对象。国际骨循环学会(Association ...目的:探讨股骨头坏死(osteonecrosis of the femoral head,ONFH)患者的疼痛特点及疼痛区域经脉循行特点。方法:选择2020年5月至2021年7月在广州中医药大学第三附属医院门诊或住院治疗的ONFH患者为研究对象。国际骨循环学会(Association Research Circulation Osseous,ARCO)分期均为Ⅰ期、Ⅱ期,男209例、女74例,年龄(42.87±14.52)岁,左侧97例、右侧81例、双侧105例,酒精性ONFH101例、激素性ONFH85例、特发性ONFH59例、创伤性ONFH38例。收集患者信息,进行ONFH临床病例信息登记。统计登记的信息,包括患者性别、年龄、病变侧别、中医证型及疼痛情况(包括性质、程度、区域、昼夜变化、是否固定、诱发因素、休息是否缓解)等;比对患者疼痛区域足三阴经和足三阳经的循行情况,统计各条经脉出现的频次;按年龄和中医证型分组,分析不同年龄和不同中医证型患者疼痛区域经脉循行的特点。结果:①疼痛情况。283例ONFH患者,疼痛性质以隐痛较常见,疼痛程度以轻中度为主且昼夜变化不明显,疼痛区域以腹股沟和股外侧最多见且位置多固定,诱发疼痛的因素有多种,大部分患者休息后疼痛能缓解。②疼痛区域经脉循行情况。283例(388髋)ONFH患者,疼痛区域有单一经脉循行186髋,有2条或2条以上经脉循行202髋;疼痛区域循行经脉出现频次较高的为足厥阴肝经和足少阳胆经。③不同年龄和不同中医证型患者疼痛区域经脉循行情况。283例ONFH患者,年龄<50岁186例,年龄≥50岁97例;气滞血瘀证56例,肾虚血瘀证142例,痰瘀互结证85例。疼痛区域循行经脉按出现频次由高至低排序,年龄<50岁组为足厥阴肝经、足少阳胆经、足太阳膀胱经、足太阴脾经、足少阴肾经、足阳明胃经,年龄≥50岁组为足少阳胆经、足厥阴肝经、足太阳膀胱经、足阳明胃经、足太阴脾经、足少阴肾经,气滞血瘀证组为足厥阴肝经、足少阳胆经、足太阳膀胱经、足阳明胃经、足太阴脾经、足少阴肾经;肾虚血瘀证组、痰瘀互结证组均为足厥阴肝经、足少阳胆经、足太阳膀胱经、足太阴脾经、足阳明胃经、足少阴肾经。结论:ARCO分期Ⅰ期、Ⅱ期ONFH患者的疼痛多为位置固定的轻中度隐痛,疼痛程度昼夜变化不明显,大部分患者休息后疼痛能缓解,疼痛区域以腹股沟和股外侧最多见;疼痛区域有足三阴经和足三阳经循行,循行经脉中足厥阴肝经和足少阳胆经最多见。展开更多
基金Supported by the National Natural Science Foundation of China(No.81200604)
文摘Objective： To investigate the electrical signals propagated along Foot Taiyang Bladder Meridian （BL） in a rat model. Methods： The experiments were performed on Dark-Agouti （DA）, DA.lU and Sprague Dawley （SD） rats. The antidromic electrical stimulation was applied on the nerve innervating ＂Pishu＂ （BL 20） to mimic the acupoint electro-acupuncture （EA）. The activities recording from adjacent nerve innervating acupoint ＂Danshu＂ （BL 19） or ＂Weishu＂ （BL 21） were recorded as indics for acupoint, including the mechanical threshold and discharge rate. Results： After mimic EA on BL 20, C and A $ units from adjacent BL 19 or BL 21 were sensitized including the decrease in mechanical threshold and increase in discharge rates in DA, DA.1U and SD rats, especially in DA rats. The average discharge rate increased from 2.40± 0.26 to 6.06± 0.55 and from 1.92±0.42 to 6.17± 1.10 impulse/min （P〈0.01）, and the mechanical threshold decreased from 0.52 ± 0.12 to 0.24 ± 0.05 and from 0.27±0.02 to 0.16±0.01 mmol/L （P〈0.01） in C （n=15） and Aδ （n=18） units in DA rats. The net change in discharge rates from C units were 152.5%, 144.7% and 42.4% in DA, DA.1U and SD rats, respectively, among which DA rat＇s was the highest （P〈0.05）. In A δ units, the net change in DA rats were also the highest （221.5%, 139.2% and 49.2% in DA, DA.lU and SD rats）. Conclusions： These results showed that mimic acupoint EA activated adjacent acupoints along BL in three rat strains, which might be related to propagated sensation along meridians （PSM）. In addition, DA rats were more sensitive and might be a good model animal for PSM research.
文摘目的:探讨股骨头坏死(osteonecrosis of the femoral head,ONFH)患者的疼痛特点及疼痛区域经脉循行特点。方法:选择2020年5月至2021年7月在广州中医药大学第三附属医院门诊或住院治疗的ONFH患者为研究对象。国际骨循环学会(Association Research Circulation Osseous,ARCO)分期均为Ⅰ期、Ⅱ期,男209例、女74例,年龄(42.87±14.52)岁,左侧97例、右侧81例、双侧105例,酒精性ONFH101例、激素性ONFH85例、特发性ONFH59例、创伤性ONFH38例。收集患者信息,进行ONFH临床病例信息登记。统计登记的信息,包括患者性别、年龄、病变侧别、中医证型及疼痛情况(包括性质、程度、区域、昼夜变化、是否固定、诱发因素、休息是否缓解)等;比对患者疼痛区域足三阴经和足三阳经的循行情况,统计各条经脉出现的频次;按年龄和中医证型分组,分析不同年龄和不同中医证型患者疼痛区域经脉循行的特点。结果:①疼痛情况。283例ONFH患者,疼痛性质以隐痛较常见,疼痛程度以轻中度为主且昼夜变化不明显,疼痛区域以腹股沟和股外侧最多见且位置多固定,诱发疼痛的因素有多种,大部分患者休息后疼痛能缓解。②疼痛区域经脉循行情况。283例(388髋)ONFH患者,疼痛区域有单一经脉循行186髋,有2条或2条以上经脉循行202髋;疼痛区域循行经脉出现频次较高的为足厥阴肝经和足少阳胆经。③不同年龄和不同中医证型患者疼痛区域经脉循行情况。283例ONFH患者,年龄<50岁186例,年龄≥50岁97例;气滞血瘀证56例,肾虚血瘀证142例,痰瘀互结证85例。疼痛区域循行经脉按出现频次由高至低排序,年龄<50岁组为足厥阴肝经、足少阳胆经、足太阳膀胱经、足太阴脾经、足少阴肾经、足阳明胃经,年龄≥50岁组为足少阳胆经、足厥阴肝经、足太阳膀胱经、足阳明胃经、足太阴脾经、足少阴肾经,气滞血瘀证组为足厥阴肝经、足少阳胆经、足太阳膀胱经、足阳明胃经、足太阴脾经、足少阴肾经;肾虚血瘀证组、痰瘀互结证组均为足厥阴肝经、足少阳胆经、足太阳膀胱经、足太阴脾经、足阳明胃经、足少阴肾经。结论:ARCO分期Ⅰ期、Ⅱ期ONFH患者的疼痛多为位置固定的轻中度隐痛,疼痛程度昼夜变化不明显,大部分患者休息后疼痛能缓解,疼痛区域以腹股沟和股外侧最多见;疼痛区域有足三阴经和足三阳经循行,循行经脉中足厥阴肝经和足少阳胆经最多见。
