Background Pediatric patients are susceptible to lung injury.Acute lung injury in children often results in high mortality.Partial liquid ventilation （PLV） has been shown to markedly improve oxygenation and reduce h...Background Pediatric patients are susceptible to lung injury.Acute lung injury in children often results in high mortality.Partial liquid ventilation （PLV） has been shown to markedly improve oxygenation and reduce histologic evidence of injury in a number of lung injury models.This study was designed to examine the hypothesis that PLV would attenuate the production of local and systemic tumor necrosis factor （TNF）-α in an immature piglet model of acute lung injury induced by oleic acid （OA）.Methods Twelve Chinese immature piglets were induced acute lung injury by OA.The animals were randomly assigned to two groups of six animals,（1） conventional mechanical ventilation （MV） group and （2） PLV with 10 ml/kg FC-77 group.Results Compared with MV group,the PLV group had better cardiopulmonary variables （P 〈0.05）.These variables included heart rate,mean blood pressure,blood pH,partial pressure of arterial oxygen （PaO2）,PaO2/inspired O2 fraction （FiO2） and partial pressure of arterial carbon dioxide （PaCO2）.PLV reduced TNF-α levels both in plasma and tissue compared with MV group （P 〈0.05）.Conclusion PLV provides protective effects against TNF-a response in OA-induced acute lung injury in immature piglets.展开更多
Background Pediatric patients are susceptible to lung injury that does not respond to traditional therapies. Total liquid ventilation has been developed as an alternative ventilatory strategy for severe lung injury. T...Background Pediatric patients are susceptible to lung injury that does not respond to traditional therapies. Total liquid ventilation has been developed as an alternative ventilatory strategy for severe lung injury. The aim of this study is to investigate the effect of total liquid ventilation on oleic acid （OA）-induced lung injury in piglets. Methods Twelve Chinese immature piglets were induced acute lung injury by OA. Twelve piglets were randomly treated with conventional gas ventilation （control group） or total liquid ventilation （study group） for 240 minutes. Samples for blood gas analysis were collected before, and at 60-minute intervals after OA-induced lung injury. The degree of lung injury was quantified by histologic examination. The inflammatory cells and the levels of IL-1β, IL-6, IL-10 and TNF-α in plasma, tissue and bronchoalveolar lavage were analyzed. Results Neutrophil and macrophage counts in bronchoalveolar lavage were significantly decreased in the study group （P〈0.05）. The total lung injury score was also reduced in the study group （P〈0.05）. The concentrations of IL-1β, IL-6, IL-10 and TNF-α in plasma, tissue and bronchoalveolar lavage were significantly reduced in the study group （P〈0.05）. Conclusions Total liquid ventilation reduces biochemical and histoloaic OA-induced luna iniurv in nialets.展开更多
目的观察部分液体通气(partial liqu id ventilation,PLV)联合气道内给予血管扩张药伊洛前列素对急性肺损伤(acute lung in jury,ALI)兔肺气体交换、肺力学方面的影响。方法健康大白兔24只,用油酸混合兔血清复制成ALI模型后随机分为3组...目的观察部分液体通气(partial liqu id ventilation,PLV)联合气道内给予血管扩张药伊洛前列素对急性肺损伤(acute lung in jury,ALI)兔肺气体交换、肺力学方面的影响。方法健康大白兔24只,用油酸混合兔血清复制成ALI模型后随机分为3组。各组采用不同治疗方法:常规机械通气组(conventionalm echan ical ventilation,CMV);部分液体通气组(PLV);联合组为PLV+雾化吸入伊洛前列素(iloprost)。分别监测各组治疗前后各时相点PaO2、PaCO2及肺静态顺应性(Cstat)、肺动脉平均压(pu lmonary arterial pressure,PAP)的变化。结果联合组PaO2从(104±16)mmHg升高到(236±38)mmHg(P<0.05),肺动脉平均压从(22±5)mmHg降低到(16±3)mmHg(P<0.05)。联合组PaO2的升高及PAP的降低优于CMV组和PLV组。结论部分液体通气联合雾化吸入伊洛前列素不仅对于改善ALI肺氧合功能优于单纯部分液体通气,还可减低部分液体通气对肺血流动力学的影响,降低肺动脉高压。展开更多
基金This study was supported by the grants from National Natural Science Foundation of China （No. 30670928 and No. 81070055）, Beijing Municipal Education Commission （No. PXM2011_014226 07 000060）, Beijing Municipal Science Committee （No. Z11110006150000） and Beijing Natural Science Foundation （No. 7122056）.We are grateful to Dr. LING Feng, Dr. LIU Ai-jun, Dr. LI Gang and Dr. XU Yu-lin for their preparation of the animals, to Dr. LIU Yang-qing and Dr. LIU De-bin for their histopathological assessment.
文摘Background Pediatric patients are susceptible to lung injury.Acute lung injury in children often results in high mortality.Partial liquid ventilation （PLV） has been shown to markedly improve oxygenation and reduce histologic evidence of injury in a number of lung injury models.This study was designed to examine the hypothesis that PLV would attenuate the production of local and systemic tumor necrosis factor （TNF）-α in an immature piglet model of acute lung injury induced by oleic acid （OA）.Methods Twelve Chinese immature piglets were induced acute lung injury by OA.The animals were randomly assigned to two groups of six animals,（1） conventional mechanical ventilation （MV） group and （2） PLV with 10 ml/kg FC-77 group.Results Compared with MV group,the PLV group had better cardiopulmonary variables （P 〈0.05）.These variables included heart rate,mean blood pressure,blood pH,partial pressure of arterial oxygen （PaO2）,PaO2/inspired O2 fraction （FiO2） and partial pressure of arterial carbon dioxide （PaCO2）.PLV reduced TNF-α levels both in plasma and tissue compared with MV group （P 〈0.05）.Conclusion PLV provides protective effects against TNF-a response in OA-induced acute lung injury in immature piglets.
基金This study was supported by grants from the National Natural Science Foundation of China （No. 81070055）, Beijing Science and Technology Commission （No. ZI 11100074911001 ）, Beijing Natural Science Foundation （No. 7112046, No. 7122056）, Beijing Health System High Level Health Technical Personnel Training Plan （No. 2011-1-4）, and Basic and Clinical Cooperation Project of Capital Medical University （No. 13JL26）.
文摘Background Pediatric patients are susceptible to lung injury that does not respond to traditional therapies. Total liquid ventilation has been developed as an alternative ventilatory strategy for severe lung injury. The aim of this study is to investigate the effect of total liquid ventilation on oleic acid （OA）-induced lung injury in piglets. Methods Twelve Chinese immature piglets were induced acute lung injury by OA. Twelve piglets were randomly treated with conventional gas ventilation （control group） or total liquid ventilation （study group） for 240 minutes. Samples for blood gas analysis were collected before, and at 60-minute intervals after OA-induced lung injury. The degree of lung injury was quantified by histologic examination. The inflammatory cells and the levels of IL-1β, IL-6, IL-10 and TNF-α in plasma, tissue and bronchoalveolar lavage were analyzed. Results Neutrophil and macrophage counts in bronchoalveolar lavage were significantly decreased in the study group （P〈0.05）. The total lung injury score was also reduced in the study group （P〈0.05）. The concentrations of IL-1β, IL-6, IL-10 and TNF-α in plasma, tissue and bronchoalveolar lavage were significantly reduced in the study group （P〈0.05）. Conclusions Total liquid ventilation reduces biochemical and histoloaic OA-induced luna iniurv in nialets.
