Patients with potential spinal stenosis are susceptible to central cord syndrome induced by blunt trauma.Suitable animal models are helpful for studying the pathogenesis and treatment of such injuries.In this study,we...Patients with potential spinal stenosis are susceptible to central cord syndrome induced by blunt trauma.Suitable animal models are helpful for studying the pathogenesis and treatment of such injuries.In this study,we established a mouse model of acute blunt traumatic spinal cord injury by compressing the C6 spinal cord with 5 and 10 g/mm~2 compression weights to simulate cervical central cord syndrome.Behavioral testing confirmed that this model exhibited the characteristics of central cord syndrome because motor function in the front paws was impaired,whereas basic motor and sensory functions of the lower extremities were retained.Hematoxylin-eosin staining showed that the diseased region of the spinal cord in this mouse model was restricted to the gray matter of the central cord,whereas the white matter was rarely affected.Magnetic resonance imaging showed a hypointense signal in the lesion after mild and severe injury.In addition,immunofluorescence staining showed that the degree of nerve tract injury in the spinal cord white matter was mild,and that there was a chronic inflammation reaction.These findings suggest that this mouse model of central cord syndrome can be used as a model for preclinical research,and that gray matter is most vulnerable to injury in central cord syndrome,leading to impaired motor function.展开更多
目的 :探讨急性创伤性中央颈髓综合征(ATCCS)手术治疗的有效性,分析影响术后脊髓功能恢复的因素。方法:回顾2015年~2017年我院骨科手术治疗的53例ATCCS患者的临床资料,包括年龄、性别、是否存在病理征、入院手内肌肌力、是否存在椎旁软...目的 :探讨急性创伤性中央颈髓综合征(ATCCS)手术治疗的有效性,分析影响术后脊髓功能恢复的因素。方法:回顾2015年~2017年我院骨科手术治疗的53例ATCCS患者的临床资料,包括年龄、性别、是否存在病理征、入院手内肌肌力、是否存在椎旁软组织损伤、脊髓内高信号长度、椎管最狭窄处百分比(MCC)、脊髓最大受压程度(MSCC)、手术时机、手术入路、入院和末次随访ASIA评分(运动、感觉)、JOA评分等信息,分析影响术后脊髓功能恢复的因素。结果:53例患者平均年龄55.57±9.68岁,39例男性(74%),14例女性(26%),伤后平均6.85±5.39天接受手术治疗,其中前路手术19例,后路手术34例,术后平均随访21.51±10.46个月;入院ASIA运动评分70.43±23.32分、ASIA感觉评分99.21±11.78分、JOA评分9.87±2.96分,末次随访ASIA运动评分92.17±13.16分、ASIA感觉评分105.77±6.79分、JOA评分14.13±3.14分,均较入院明显增加(P<0.05),脊髓功能明显恢复。根据JOA评分改善率分组(>50%定义为预后好39例,≤50%定义为预后差14例)并进行相关因素分析,结果显示高龄(61.14±7.55 vs 53.56±9.65岁)、入院手内肌肌力较差(5.43±5.95 vs 9.49±4.99分)、病理征阳性(阳性/阴性)(10/4 vs 15/24例),MRI T2像显示存在椎旁软组织损伤(有/无)(8/6 vs 9/30例)及脊髓内高信号长度较长(25.87±13.85mm vs 18.08±10.68mm)是预后差的主要因素(P<0.05)。而性别(男/女)(29/10vs 10/4例)、MCC[(43.18±10.96)%vs (49.14±7.94)%]、MSCC[(26.75±10.81)%vs (28.67±9.59)%]、入院ASIA运动评分(74.18±22.78 vs 60.00±22.35分)、入院ASIA感觉评分(101.38±8.93 vs 93.14±16.38分)、手术时机(7.51±5.87 vs 5.00±3.23分)、手术入路(前路/后路)(15/24 vs 4/10例)对预后无明显影响(P>0.05)。结论 :ATCCS采用手术治疗安全有效;高龄、病理征阳性、入院手内肌评分低、椎旁软组织有损伤、脊髓内高信号长度长提示患者术后脊髓功能恢复差。展开更多
基金the National Key Research and Development Project of Stem Cell and Transformation Research,No.2019YFA0112100the National Natural Science Foundation of China(Key Program),No.81930070(both to SF)。
文摘Patients with potential spinal stenosis are susceptible to central cord syndrome induced by blunt trauma.Suitable animal models are helpful for studying the pathogenesis and treatment of such injuries.In this study,we established a mouse model of acute blunt traumatic spinal cord injury by compressing the C6 spinal cord with 5 and 10 g/mm~2 compression weights to simulate cervical central cord syndrome.Behavioral testing confirmed that this model exhibited the characteristics of central cord syndrome because motor function in the front paws was impaired,whereas basic motor and sensory functions of the lower extremities were retained.Hematoxylin-eosin staining showed that the diseased region of the spinal cord in this mouse model was restricted to the gray matter of the central cord,whereas the white matter was rarely affected.Magnetic resonance imaging showed a hypointense signal in the lesion after mild and severe injury.In addition,immunofluorescence staining showed that the degree of nerve tract injury in the spinal cord white matter was mild,and that there was a chronic inflammation reaction.These findings suggest that this mouse model of central cord syndrome can be used as a model for preclinical research,and that gray matter is most vulnerable to injury in central cord syndrome,leading to impaired motor function.
文摘目的 :探讨急性创伤性中央颈髓综合征(ATCCS)手术治疗的有效性,分析影响术后脊髓功能恢复的因素。方法:回顾2015年~2017年我院骨科手术治疗的53例ATCCS患者的临床资料,包括年龄、性别、是否存在病理征、入院手内肌肌力、是否存在椎旁软组织损伤、脊髓内高信号长度、椎管最狭窄处百分比(MCC)、脊髓最大受压程度(MSCC)、手术时机、手术入路、入院和末次随访ASIA评分(运动、感觉)、JOA评分等信息,分析影响术后脊髓功能恢复的因素。结果:53例患者平均年龄55.57±9.68岁,39例男性(74%),14例女性(26%),伤后平均6.85±5.39天接受手术治疗,其中前路手术19例,后路手术34例,术后平均随访21.51±10.46个月;入院ASIA运动评分70.43±23.32分、ASIA感觉评分99.21±11.78分、JOA评分9.87±2.96分,末次随访ASIA运动评分92.17±13.16分、ASIA感觉评分105.77±6.79分、JOA评分14.13±3.14分,均较入院明显增加(P<0.05),脊髓功能明显恢复。根据JOA评分改善率分组(>50%定义为预后好39例,≤50%定义为预后差14例)并进行相关因素分析,结果显示高龄(61.14±7.55 vs 53.56±9.65岁)、入院手内肌肌力较差(5.43±5.95 vs 9.49±4.99分)、病理征阳性(阳性/阴性)(10/4 vs 15/24例),MRI T2像显示存在椎旁软组织损伤(有/无)(8/6 vs 9/30例)及脊髓内高信号长度较长(25.87±13.85mm vs 18.08±10.68mm)是预后差的主要因素(P<0.05)。而性别(男/女)(29/10vs 10/4例)、MCC[(43.18±10.96)%vs (49.14±7.94)%]、MSCC[(26.75±10.81)%vs (28.67±9.59)%]、入院ASIA运动评分(74.18±22.78 vs 60.00±22.35分)、入院ASIA感觉评分(101.38±8.93 vs 93.14±16.38分)、手术时机(7.51±5.87 vs 5.00±3.23分)、手术入路(前路/后路)(15/24 vs 4/10例)对预后无明显影响(P>0.05)。结论 :ATCCS采用手术治疗安全有效;高龄、病理征阳性、入院手内肌评分低、椎旁软组织有损伤、脊髓内高信号长度长提示患者术后脊髓功能恢复差。
