We report the expression profile of acyl-lipid △12-desaturase (desA) gene from Synechocystis sp. PCC6803 and its effect on cell membrane lipid composition and cold tolerance in prokaryotic (Escherichia coil) and ...We report the expression profile of acyl-lipid △12-desaturase (desA) gene from Synechocystis sp. PCC6803 and its effect on cell membrane lipid composition and cold tolerance in prokaryotic (Escherichia coil) and eukaryotic (Solanum tuberosum) cells. For this purpose, a hybrid of desA and reporter gene encoding thermostable lichenase (licBM3) was constructed and used to transform these cells. The expression of this hybrid gene was measured using qualitative (Petri dish test, electrophoregram and zymogram) and quantitative methods (spectrometry and gas liquid chromatography assays). The maximum level of linoleic acid in the bacterial cells containing hybrid gene was 1.9% of total fatty acids. Cold stress tolerance assays using plant damage index and growth parameters showed that cold tolerance was enhanced in primary transgenic lines because of increased unsaturated fatty acid concentration in their lipids. The greatest content of 18:2 and 18:3 fatty acids in primary transgenic plants was observed for lines 2 (73%) and 3 (41%). Finally, our results showed that desaturase could enhance tolerance to cold stress in potato, and desaturase and lichenase retain their functionality in the structure of the hybrid protein where the enzymatic activity of target gene product was higher than in the case of reporter lichenase gene absence in the construction.展开更多
目的研究1例以转氨酶升高为主的多种酰基辅酶A脱氢酶缺乏症患者临床表现、实验室检查、肌肉活检及基因突变情况分析,并进行文献复习,为该病的早期诊断及治疗提供依据。方法收集1例7岁3月男性患儿的临床资料,采集患儿及父母血标本,采用...目的研究1例以转氨酶升高为主的多种酰基辅酶A脱氢酶缺乏症患者临床表现、实验室检查、肌肉活检及基因突变情况分析,并进行文献复习,为该病的早期诊断及治疗提供依据。方法收集1例7岁3月男性患儿的临床资料,采集患儿及父母血标本,采用二代基因测序检测致病基因,腓肠肌穿刺活检明确肌肉病变情况。结果患儿肌肉活检电镜结果示肌纤维内大量脂滴沉积。基因测序结果显示患儿的ETFDH基因存在c.1773_1774del AT p.(Cys592※)无义突变和c.389A>T p.(Asp130Val)错义突变,考虑为复合杂合突变,患儿父母分别为携带者。结论临床上有转氨酶升高为主伴有心肌酶升高、运动障碍者,应尽早进行分子遗传学检查,有条件者行腓肠肌肌肉活检术,可以为患儿家庭提供准确的遗传咨询和产前诊断。展开更多
基金supported by the program "Biological Diversity–Gene Foundation and Genetic Diversity" of the Presidium of the Russian Academy of Sciences and the Russian Foundation for Basic Research (project nos. 08-04-90410-Ykp-a, 05-04-49186-a, 04-04-81039-Bel_a)
文摘We report the expression profile of acyl-lipid △12-desaturase (desA) gene from Synechocystis sp. PCC6803 and its effect on cell membrane lipid composition and cold tolerance in prokaryotic (Escherichia coil) and eukaryotic (Solanum tuberosum) cells. For this purpose, a hybrid of desA and reporter gene encoding thermostable lichenase (licBM3) was constructed and used to transform these cells. The expression of this hybrid gene was measured using qualitative (Petri dish test, electrophoregram and zymogram) and quantitative methods (spectrometry and gas liquid chromatography assays). The maximum level of linoleic acid in the bacterial cells containing hybrid gene was 1.9% of total fatty acids. Cold stress tolerance assays using plant damage index and growth parameters showed that cold tolerance was enhanced in primary transgenic lines because of increased unsaturated fatty acid concentration in their lipids. The greatest content of 18:2 and 18:3 fatty acids in primary transgenic plants was observed for lines 2 (73%) and 3 (41%). Finally, our results showed that desaturase could enhance tolerance to cold stress in potato, and desaturase and lichenase retain their functionality in the structure of the hybrid protein where the enzymatic activity of target gene product was higher than in the case of reporter lichenase gene absence in the construction.
文摘目的研究1例以转氨酶升高为主的多种酰基辅酶A脱氢酶缺乏症患者临床表现、实验室检查、肌肉活检及基因突变情况分析,并进行文献复习,为该病的早期诊断及治疗提供依据。方法收集1例7岁3月男性患儿的临床资料,采集患儿及父母血标本,采用二代基因测序检测致病基因,腓肠肌穿刺活检明确肌肉病变情况。结果患儿肌肉活检电镜结果示肌纤维内大量脂滴沉积。基因测序结果显示患儿的ETFDH基因存在c.1773_1774del AT p.(Cys592※)无义突变和c.389A>T p.(Asp130Val)错义突变,考虑为复合杂合突变,患儿父母分别为携带者。结论临床上有转氨酶升高为主伴有心肌酶升高、运动障碍者,应尽早进行分子遗传学检查,有条件者行腓肠肌肌肉活检术,可以为患儿家庭提供准确的遗传咨询和产前诊断。