期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
Autophagy induced by human adenovirus B7 structural protein VI inhibits viral replication
1
作者 Linlin Zhang Yali Duan +5 位作者 Wei Wang Qi Li Jiao Tian Yun Zhu Ran Wang Zhengde Xie 《Virologica Sinica》 SCIE CAS CSCD 2023年第5期709-722,共14页
Human adenovirus B7(HAdV-B7)causes severe acute lower respiratory tract infections in children.However,neither the child-specific antivirals or vaccines are available,nor the pathogenesis is clear.Autophagy,as part of... Human adenovirus B7(HAdV-B7)causes severe acute lower respiratory tract infections in children.However,neither the child-specific antivirals or vaccines are available,nor the pathogenesis is clear.Autophagy,as part of innate immunity,plays an important role in resistance to viral infection by degrading the virus and promoting the development of innate and adaptive immunity.This study provided evidence that HAdV-B7 infection induced complete autophagic flux,and the pharmacological induction of autophagy decreased HAdV-B7 replication.In this process,the host protein Bcl2-associated athanogene 3(BAG3)mediated autophagy to inhibit the replication of HAdV-B7 by binding to the PPSY structural domain of viral protein pVI through its WW structural domain.These findings further our understanding of the host immune response during viral infection and will help to develop broad anti-HAdV therapies. 展开更多
关键词 Human adenovirus B7(HAdV-B7) AUTOPHAGY Bcl2-associated athanogene 3(BAG3) Virus replication
原文传递
蟾毒内酯抗柯萨奇病毒、腺病毒和流感病毒作用的研究 被引量:5
2
作者 孙非 张淑芹 +2 位作者 刘志屹 靳玉琴 毋英杰 《中国药理学通报》 CAS CSCD 北大核心 2004年第4期478-479,共2页
关键词 蟾毒内酯 柯萨奇病毒B3 腺病毒7型(Ad7) 流感病毒(FM1)
下载PDF
Genetic Analysis of Human Adenovirus Type 7 Strains Circulating in Different Parts of China 被引量:2
3
作者 Yali Duan Changchong Li +8 位作者 Li Deng Shuhua An Yun Zhu Wei Wang Meng Zhang Lili Xu Baoping Xu Xiangpeng Chen Zhengde Xie 《Virologica Sinica》 SCIE CAS CSCD 2021年第3期382-392,共11页
To investigate the molecular epidemiology and genetic variation of human adenovirus type 7(HAdV-7)in children with acute respiratory infections(ARI)in China.HAdV-7-positive respiratory samples collected from children ... To investigate the molecular epidemiology and genetic variation of human adenovirus type 7(HAdV-7)in children with acute respiratory infections(ARI)in China.HAdV-7-positive respiratory samples collected from children with ARI in Beijing,Shijiazhuang,Wenzhou and Guangzhou from 2014–2018 were selected for gene amplification and sequence analysis.Fifty-seven HAdV-7 clinical strains with hexon,penton base and fiber gene sequences were obtained.Meanwhile17 strains were selected randomly from different cities for whole genome sequencing.Phylogenetic and variation analyses were performed based on the obtained sequences,HAdV-7 prototype strain Gomen(AY594255),vaccine strains(AY495969 and AY594256)and representative sequences of strains.The phylogenetic trees constructed based on whole genome sequences,major capsid protein genes(hexon,penton base and fiber)and the early genes(E1,E2,E3 and E4)were not completely consistent.The HAdV-7 strains obtained in this study always clustered with most of the circulating strains worldwide from the 1980 s to the present.Compared with the HAdV-7 prototype strain Gomen(AY594255),some amino acid mutations in loop1 and loop2 of hexon and the RGD loop region of the penton base gene were observed.Recombination analysis showed that partial regions of 55 k Da protein and 100 kDa hexon-assembly associated protein genes among all HAdV-7 strains in this study were from HAdV-16 and HAdV-3,respectively.Our study demonstrated the molecular evolution characteristics of HAdV-7 strains circulating in China and provided basic reference data for the prevention,control and vaccine development of HAdV-7. 展开更多
关键词 Human adenovirus type 7(HAdV-7) Genome-wide sequence analysis Mutation Genetic recombination
原文传递
人腺病毒7型DNA结合蛋白的原核表达及多克隆抗体制备 被引量:2
4
作者 朱云 张林琳 +1 位作者 段亚丽 谢正德 《中华预防医学杂志》 CAS CSCD 北大核心 2022年第2期171-177,共7页
目的原核表达人腺病毒(HAdV)7型DNA结合蛋白(DBP),制备DBP蛋白多克隆抗体。方法合成HAdV-7 DBP基因并克隆至pET30a载体,转化大肠杆菌BL21(DE3)感受态细胞,IPTG诱导进行原核表达。经镍离子金属螯合层析纯化后免疫家兔制备多克隆抗体,使... 目的原核表达人腺病毒(HAdV)7型DNA结合蛋白(DBP),制备DBP蛋白多克隆抗体。方法合成HAdV-7 DBP基因并克隆至pET30a载体,转化大肠杆菌BL21(DE3)感受态细胞,IPTG诱导进行原核表达。经镍离子金属螯合层析纯化后免疫家兔制备多克隆抗体,使用间接酶联免疫吸附试验(ELISA)测定该抗体效价;使用Western blotting方法与间接免疫荧光方法(IFA)检测抗体特异性。以rDBP包被ELISA反应板使用间接ELISA对HAdV感染儿童急性期血清中抗DBP蛋白IgM和IgG抗体进行检测。结果成功构建HAdV-7 DBP原核表达载体,表达的重组DBP蛋白经镍柱亲和层析纯化后纯度>95%,间接ELISA方法测得制备的多克隆抗血清的抗体效价为1∶1024000,Western blotting方法和IFA方法显示该抗体具有较高的特异性,间接ELISA方法检测HAdV感染儿童急性期血清中抗DBP蛋白IgM和IgG抗体的阳性率分别为50.0%(19/38)和63.2%(24/38)。结论成功构建HAdV-7 DBP蛋白的原达表达载体,获得了HAdV-7型DBP重组蛋白和高效价的兔多克隆抗体。 展开更多
关键词 人腺病毒7 DNA结合蛋白 原核表达 多克隆抗体
原文传递
Analysis of severe human adenovirus infection outbreak in Guangdong Province,southern China in 2019 被引量:8
5
作者 Wenkuan Liu Shuyan Qiu +9 位作者 Li Zhang Hongkai Wu Xingui Tian Xiao Li Duo Xu Jing Dai Shujun Gu Qian Liu Dehui Chen Rong Zhou 《Virologica Sinica》 SCIE CAS CSCD 2022年第3期331-340,共10页
During 2018–2019,a severe human adenovirus(HAdV)infection outbreak occurred in southern China.Here,we screened 18 respiratory pathogens in 1704 children(≤14 years old)hospitalized with acute respiratory illness in G... During 2018–2019,a severe human adenovirus(HAdV)infection outbreak occurred in southern China.Here,we screened 18 respiratory pathogens in 1704 children(≤14 years old)hospitalized with acute respiratory illness in Guangzhou,China,in 2019.In total,151 patients had positive HAdV test results;34.4%(52/151)of them exhibited severe illness.HAdV infection occurred throughout the year,with a peak in summer.The median patient age was 3.0(interquartile range:1.1–5.0)years.Patients with severe HAdV infection exhibited increases in12 clinical indexes(P≤0.019)and decreases in four indexes(P≤0.007),compared with patients exhibiting nonsevere infection.No significant differences were found in age or sex distribution according to HAdV infection severity(P>0.05);however,the distributions of comorbid disease and HAdV co-infection differed according to HAdV infection severity(P<0.05).The main epidemic types were HAdV-3(47.0%,71/151)and HAdV-7(46.4%,70/151).However,the severe illness rate was significantly higher in patients with HAdV-7(51.4%)than in patients with HAdV-3(19.7%)and other types of HAdV(20%)(P<0.001).Sequencing analysis of genomes/capsid genes of 13 HAdV-7 isolates revealed high similarity to previous Chinese isolates.A representative HAdV-7isolate exhibited a similar proliferation curve to the curve described for the epidemic HAdV-3 strain Guangzhou01(accession no.DQ099432)(P>0.05);the HAdV-7 isolate exhibited stronger virulence and infectivity,compared with HAdV-3(P<0.001).Overall,comorbid disease,HAdV co-infection,and high virulence and infectivity of HAdV-7 were critical risk factors for severe HAdV infection;these data can facilitate treatment,control,and prevention of HAdV infection. 展开更多
关键词 Human adenovirus(HAdV) Human adenovirus type 7(HAdV-7) Severe illness EPIDEMIOLOGY Acute respiratory illness Virulence and infectivity Risk factor
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部