期刊文献+
共找到17,550篇文章
< 1 2 250 >
每页显示 20 50 100
Mouse KL2 is a unique MTSE involved in chromosome-based spindle organization and regulated by multiple kinases during female meiosis
1
作者 Shiya Xie Yanjie Yang +8 位作者 Zhen Jin Xiaocong Liu Shuping Zhang Ning Su Jiaqi Liu Congrong Li Dong Zhang Leilei Gao Zhixia Yang 《Journal of Biomedical Research》 CAS CSCD 2024年第5期485-499,I0009-I0011,共18页
Microtubule-severing enzymes(MTSEs)play important roles in mitosis and meiosis of the primitive organisms.However,their roles in mammalian female meiosis,which accounts for over 80%of gamete-originated human reproduct... Microtubule-severing enzymes(MTSEs)play important roles in mitosis and meiosis of the primitive organisms.However,their roles in mammalian female meiosis,which accounts for over 80%of gamete-originated human reproductive diseases,remain unexplored.In the current study,we reported that katanin-like 2(KL2)was the only MTSE concentrating at chromosomes.Furthermore,the knockdown of KL2 significantly reduced the chromosome-based increase in the microtubule(MT)polymer,increased aberrant kinetochore-MT(K-MT)attachment,delayed meiosis,and severely affected normal fertility.We demonstrated that the inhibition of aurora B,a key kinase for correcting aberrant K-MT attachment,significantly eliminated KL2 expression from chromosomes.Additionally,KL2 interacted with phosphorylated eukaryotic elongation factor-2 kinase,and they competed for chromosome binding.Phosphorylated KL2 was also localized at spindle poles,with its phosphorylation regulated by extracellular signal-regulated kinase 1/2.In summary,the current study reveals a novel function of MTSEs in mammalian female meiosis and demonstrates that multiple kinases coordinate to regulate the levels of KL2 at chromosomes. 展开更多
关键词 mouse KL2 MTSE KINASE female meiosis
下载PDF
Role of cancer stem cell ecosystem on breast cancer metastasis and related mouse models
2
作者 Xilei Peng Haonan Dong +1 位作者 Lixing Zhang Suling Liu 《Zoological Research》 SCIE CSCD 2024年第3期506-517,共12页
Breast cancer metastasis is responsible for most breast cancer-related deaths and is influenced by many factors within the tumor ecosystem,including tumor cells and microenvironment.Breast cancer stem cells(BCSCs)cons... Breast cancer metastasis is responsible for most breast cancer-related deaths and is influenced by many factors within the tumor ecosystem,including tumor cells and microenvironment.Breast cancer stem cells(BCSCs)constitute a small population of cancer cells with unique characteristics,including their capacity for self-renewal and differentiation.Studies have shown that BCSCs not only drive tumorigenesis but also play a crucial role in promoting metastasis in breast cancer.The tumor microenvironment(TME),composed of stromal cells,immune cells,blood vessel cells,fibroblasts,and microbes in proximity to cancer cells,is increasingly recognized for its crosstalk with BCSCs and role in BCSC survival,growth,and dissemination,thereby influencing metastatic ability.Hence,a thorough understanding of BCSCs and the TME is critical for unraveling the mechanisms underlying breast cancer metastasis.In this review,we summarize current knowledge on the roles of BCSCs and the TME in breast cancer metastasis,as well as the underlying regulatory mechanisms.Furthermore,we provide an overview of relevant mouse models used to study breast cancer metastasis,as well as treatment strategies and clinical trials addressing BCSC-TME interactions during metastasis.Overall,this study provides valuable insights for the development of effective therapeutic strategies to reduce breast cancer metastasis. 展开更多
关键词 Breast cancer METASTASIS Cancer stem cell ECOSYSTEM Tumor microenvironment mouse model
下载PDF
Comparison of immune responses and intestinal flora in epicutaneously sensitized BALB/c or C57BL/6 mouse models of food allergy
3
作者 Gang Yu Yuhao Jiang +6 位作者 Shuifeng Zhang Pengpeng Liu Shunyu Wang Huadong Sheng Yanbo Wang Qiaozhi Zhang Linglin Fu 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期668-680,共13页
Cutaneous exposure to food allergens through a disrupted skin barrier is recognized as an important cause of food allergy,and the cutaneous sensitized mouse model has been established to investigate relevant allergic ... Cutaneous exposure to food allergens through a disrupted skin barrier is recognized as an important cause of food allergy,and the cutaneous sensitized mouse model has been established to investigate relevant allergic disorders.However,the role of different genetic backgrounds of mice on immune responses to food allergens upon epicutaneous sensitization is largely unknown.In this study,two strains of mice,i.e.,the BALB/c and C57BL/6 mice,were epicutaneously sensitized with ovalbumin on atopic dermatitis(AD)-like skin lesions,followed by intragastric challenge to induce IgE-mediated food allergy.Allergic outcomes were measured as clinical signs,specific antibodies and cytokines,and immune cell subpopulations,as well as changes in intestinal barrier function and gut microbiota.Results showed that both strains of mice exhibited typical food-allergic symptoms with a Th2-skewed response.The C57BL/6 mice,rather than the BALB/c mice,were fitter for establishing an epicutaneously sensitized model of food allergy since a stronger Th2-biased response and severer disruptions in the intestinal barrier and gut homeostasis were observed.This study provides knowledge for selecting an appropriate mouse model to study food-allergic responses associated with AD-like skin lesions and highlights the role of genetic variations in the immune mechanism underlying pathogenesis of food allergy. 展开更多
关键词 Food allergy mouse models Epicutaneous sensitization Th2 response Gut microbiota
下载PDF
Establishment and Evaluation of a Mouse Model of Allergic Rhinitis
4
作者 Xiang QIN Yuan PAN +7 位作者 Xiaoni PAN Yanfeng MO Shuwen LI Tingting XIE Lixiang LU Jiancheng LIANG Shiyou ZHOU Suoyi HUANG 《Medicinal Plant》 2024年第4期111-113,121,共4页
[Objectives]To explore a new method for induction of allergic rhinitis in mice,and compare and evaluate it with common modeling methods.[Methods]36 mice were randomly divided into the control group,blank group and exp... [Objectives]To explore a new method for induction of allergic rhinitis in mice,and compare and evaluate it with common modeling methods.[Methods]36 mice were randomly divided into the control group,blank group and experimental group,and there were 12 mice in each group.The mice in the control group were conventionally induced.That is,the mice were first injected intraperitoneally with the mixture composed of OVA 50μg,[Al(OH)3]5 mg and 1ml of normal saline once every other day,and then since the 15 th d,20μL of 5%OVA solution was dropped into each nasal cavity once a day,which lasted for 7 d.The blank group was treated with the same amount of normal saline according to the control group,and received intraperitoneal injection and bilateral nasal drip respectively.In the experimental group,mice were first given intraperitoneal injection of the mixture composed of ovalbumin(OVA)75μg,aluminum hydroxide gel[Al(OH)3]8 mg and normal saline 1.5 mL for basic sensitization.On the 26 th d,20μL of 3%OVA solution was dropped into each nasal cavity once a day,which lasted for 10 d.The number of sneezes,the number of nose scratching,the amount of nasal discharge,and the activity of mice in each group were observed,and the behavior of allergic reaction was scored.Meanwhile,the number of eosinophils in the nasal discharge of mice and the IgE content in serum were measured.[Results]The score of nasal stimulation symptoms,the number of eosinophils and serum IgE level of mice in the control group and the experimental group were higher than those in the blank group(P<0.05),and there was no statistical significance between the two groups in the three indicators(P>0.05).[Conclusions]The modeling method was more suitable for the development of allergic rhinitis patients condition,and reduced the probability of death of mice due to modeling,and simplified the experimental operation. 展开更多
关键词 Allergic rhinitis mouse model Modeling effect
下载PDF
Expression levels of K_(ATP)channel subunits and morphological changes in the mouse liver after exposure to radiation
5
作者 Ming Zhou Tao-Sheng Li +3 位作者 Hiroshi Abe Hideo Akashi Ryoji Suzuki Yoshio Bando 《World Journal of Experimental Medicine》 2024年第2期103-113,共11页
BACKGROUND ATP sensitive K+(K_(ATP))channels are ubiquitously distributed in various of cells and tissues,including the liver.They play a role in the pathogenesis of myocardial and liver ischemia.AIM To evaluate the r... BACKGROUND ATP sensitive K+(K_(ATP))channels are ubiquitously distributed in various of cells and tissues,including the liver.They play a role in the pathogenesis of myocardial and liver ischemia.AIM To evaluate the radiation-induced changes in the expression of K_(ATP)channel subunits in the mouse liver to understand the potential role of K_(ATP)channels in radiation injury.METHODS Adult C57BL/6 mice were randomly exposed toγ-rays at 0 Gy(control,n=2),0.2 Gy(n=6),1 Gy(n=6),or 5 Gy(n=6).The livers were removed 3 and 24 h after radiation exposure.Hematoxylin and eosin staining was used for morphological observation;immunohistochemical staining was applied to determine the expression of K_(ATP)channel subunits in the liver tissue.RESULTS Compared with the control group,the livers exposed to 0.2 Gyγ-ray showed an initial increase in the expression of Kir6.1 at 3 h,followed by recovery at 24 h after exposure.Exposure to a high dose of 5.0 Gy resulted in decreased expression of Kir6.1 and increased expression of SUR2B at 24 h.However,the expression of Kir6.2,SUR1,or SUR2A had no remarkable changes at 3 and 24 h after exposure to any of these doses.CONCLUSION The expression levels of Kir6.1 and SUR2B in mouse liver changed differently in response to different radiation doses,suggesting a potential role for them in radiation-induced liver injury. 展开更多
关键词 Radiation exposure ATP-sensitive K^(+)channel mouse LIVER
下载PDF
Effects of Polygala fallax Hemsl Water Extract on a Mouse Model of Gastric Motility Disorders
6
作者 Liangyin ZHU Xiaofeng HAO +8 位作者 Liuqi HUANG Zongyuan CHEN Wenfeng CHEN Liu YANG Bingchen HUANG Liyuan LI Aipeng LI Suoyi HUANG Xiuzhen SU 《Medicinal Plant》 2024年第4期101-106,共6页
[Objectives]To explore the effects of Polygona fallax Hemsl water extract on gastrointestinal motility in normal mice and gastric motility disorder model mice and approximate mechanism.[Methods]Using normal mice and m... [Objectives]To explore the effects of Polygona fallax Hemsl water extract on gastrointestinal motility in normal mice and gastric motility disorder model mice and approximate mechanism.[Methods]Using normal mice and mice with gastric motility disorders(modeled with atropine),the effects of different mass concentration groups of P.fallax Hemsl water extract(0.125,0.250,0.500 g/mL)and domperidone groups on gastric residual rate,small intestine propulsion rate,serum motilin(MLT),vasoactive intestinal peptide(VIP),and tissue morphology were studied.[Results]There was a highly significant difference(P<0.01)in the small intestine propulsion rate of liquid in normal mice among the different concentration groups of P.fallax Hemsl water extract compared to the blank group.The small intestine propulsion rate and gastric residue rate of semi-solid paste were statistically significant compared to the blank group(P<0.05).Among them,there was a highly significant difference between the high concentration group(67.75%±7.65%,46.5%±10.62%)and the medium concentration group(60.90%±5.87%,59.27%±7.82%)(P<0.01).There was statistical significance in normal mouse serum MLT content in the high concentration group(P<0.05).There was no effect on serum VIP levels in normal mice;no effect on the morphology of stomach and intestinal tissues of normal mice.The small intestine propulsion rate and gastric residue rate of liquid and semi-solid paste in mice with gastric motility disorders were statistically significant compared to the atropine group,with extremely significant differences(P<0.01).[Conclusions]P.fallax Hemsl water extract has a promoting effect on gastrointestinal motility.One of the specific mechanisms by which P.fallax Hemsl promotes gastrointestinal motility in normal mice may be related to the content of MLT in mouse serum.The mechanism of action in atropine induced gastric paresis mice may be related to the reactivation of M receptors,and the action mechanism of P.fallax Hemsl does not change the original histological basis.It can be inferred that P.fallax Hemsl water extract has a synergistic effect on promoting gastrointestinal motility through other mechanisms,but it is not fully understood and further in-depth research is needed. 展开更多
关键词 Polygala fallax Hemsl Gastric motility mouse model Gastric emptying Gastric paresis MOTILIN Vasoactive intestinal peptide
下载PDF
Effect of Weak Noise on the Frequency Tuning of Mouse Inferior Collicular Neurons 被引量:8
7
作者 唐佳 皮建辉 +2 位作者 王丹 吴飞健 陈其才 《Zoological Research》 CAS CSCD 北大核心 2004年第3期191-197,共7页
In order to study the effect of weak noise on the sound signal extraction of mouse (Mus musculus Km) inferior collicular (IC) neurons from environments,we examined the changes in frequency tuning curves (FTCs) of 32 n... In order to study the effect of weak noise on the sound signal extraction of mouse (Mus musculus Km) inferior collicular (IC) neurons from environments,we examined the changes in frequency tuning curves (FTCs) of 32 neurons induced by a weak noise relative to 5 dB below minimum threshold of tone (reMT-5 dB) under free field stimulation conditions.The results were as follows:① There were three types of variations in FTCs,sharpened (34.4%),broadened (18.8%),and unaffected (46.9%),nevertheless,only the alteration of sharpened FTCs was statistically different.② Sharpness of frequency tuning induced by a reMT-5 dB noise was very strong.Q 10 and Q 30 of FTCs were increased by (34.42±17.04)% (P=0.026,n=11) and (46.34±22.88)% (P=0.009,n=7).③ The changes of inverse-slopes (ISs,kHz/dB) between high (IS high) and low (IS low) limbs of FTCs were dissymmetry.The IS high of FTCs decreased markedly (P=0.046,n=7),however,there was little change (P=0.947,n=7) in IS low.Our data revealed for the first time that the weak noise could sharpen frequency tuning and increase the sensitivity on the high frequency of sound signal in IC neurons of mouse. 展开更多
关键词 Weak white noise Sharpening frequency tuning Inferior collicular neurons mouse
下载PDF
Misère规则下Mouse游戏的最优策略 被引量:1
8
作者 刘文安 马迎宾 +1 位作者 李海锋 李蓓 《河南师范大学学报(自然科学版)》 CAS CSCD 北大核心 2010年第6期171-173,共3页
研究misère规则下一种新的公平组合游戏——Mouse游戏.确定出Mouse游戏的所有P位置,从而得到Mouse游戏的最优策略.
关键词 mouse游戏 P位置 misère规则
下载PDF
脊柱测量尺和Spinal Mouse脊柱测量仪测量胸椎后凸角、腰椎前凸角的信度和效度 被引量:13
9
作者 冯强 周誉 《中国运动医学杂志》 CAS CSCD 北大核心 2017年第2期150-155,共6页
目的:探讨脊柱测量尺和Spinal Mouse脊柱测量仪在测量胸椎后凸角、腰椎前凸角两个指标中的信度和效度。方法:随机筛选29名高中生作为受试对象,其中14名男生,15名女生。使用侧位全脊柱X线片作为胸椎后凸角和腰椎前凸角测试的金标准,分别... 目的:探讨脊柱测量尺和Spinal Mouse脊柱测量仪在测量胸椎后凸角、腰椎前凸角两个指标中的信度和效度。方法:随机筛选29名高中生作为受试对象,其中14名男生,15名女生。使用侧位全脊柱X线片作为胸椎后凸角和腰椎前凸角测试的金标准,分别使用Spinal Mouse脊柱测量仪、脊柱测量尺(Flexible ruler)对胸椎后凸角和腰椎前凸角进行测量。检验Spinal Mouse脊柱测量仪和脊柱测量尺测量脊柱矢状面角度的效度与信度,并检验两种仪器的重测信度。结果:Spinal Mouse脊柱测量仪测量的胸椎后凸角与X线片一致性最高,ICC为0.803(0.622,0.902),脊柱测量尺测量的胸椎后凸角ICC值为0.753(0.538,0.876);脊柱测量尺和Spinal Mouse脊柱测量仪测量的腰椎前凸角与X线片的测量结果相比,ICC值均低于0.4,一致性不高。在重复测量分析方面,使用Spinal Mouse脊柱测量仪和脊柱测量尺测量胸椎后凸角的ICC均高于0.8,使用Spinal Mouse测量腰椎前凸角的ICC为0.809(0.633,0.906),而使用脊柱测量尺测量腰椎前凸角的ICC为0.704(0.459,0.849)。结论:Spinal Mouse脊柱测量仪和脊柱测量尺在测试胸椎后凸角和腰椎前凸角中均具备良好的重测信度。与X线片对比,Spinal Mouse脊柱测量仪和脊柱测量尺测量胸椎后凸角具备一定的效度,可以在一定程度上替代X线片作为测量方法,且Spinal Mouse脊柱测量仪优于脊柱测量尺。但两种测试方法测量腰椎前凸角效度不佳。 展开更多
关键词 脊柱测量尺 SPINAL mouse脊柱测量仪 胸椎后凸角 腰椎前凸角 X线片 信度 效度
下载PDF
基于微加速度计的AIR-MOUSE的研究 被引量:7
10
作者 姜晓波 陈文元 +2 位作者 汤坚 钱莉 凌灵 《微计算机信息》 北大核心 2008年第22期113-115,共3页
本文研究了一种基于微加速度计原理的空中鼠标。该鼠标采用微加速度计来检测手部运动的角度和速度等参数,并通过射频无线传输和USB接口实现与计算机的通信。本文介绍微加速度计、微控制器、无线射频模块、USB通讯等的集成研究,并对无线... 本文研究了一种基于微加速度计原理的空中鼠标。该鼠标采用微加速度计来检测手部运动的角度和速度等参数,并通过射频无线传输和USB接口实现与计算机的通信。本文介绍微加速度计、微控制器、无线射频模块、USB通讯等的集成研究,并对无线传输模块的软硬件做了详细阐述。 展开更多
关键词 微加速度计 无线射频 USB接口 空中鼠标
下载PDF
Construction of Mouse Nutritional Obesity Model
11
作者 田方 金红星 成文玉 《Animal Husbandry and Feed Science》 CAS 2009年第1期5-8,共4页
[ Objective] The aim of this study is to construct the model for simple obesity induced by high-fat diet, which is closest to human obesity, laying a foundation for the studies of obesity related theories. [Method[ IC... [ Objective] The aim of this study is to construct the model for simple obesity induced by high-fat diet, which is closest to human obesity, laying a foundation for the studies of obesity related theories. [Method[ ICR and KM mice, half male and half female, were randomly divided into the high-fat diet experimental group and the normal diet control group based on body weights, and certain days later, body weight, Lee' s index, wet weight of adipose tissue, quantity of adipose cell in the same visual field and blood indices were measured. [Result]All indices mentioned a- bove of the female I CR mouse had significant statistical differences with those of the control group (P 〈 0.01 or P 〈 0.05). [ Conclusion] To con- struct mouse nutritional obesity model successfully, different high-fat diets are required by different lines as well as different sexes in the same line. 展开更多
关键词 mouse Nutritional obesity High-fat diet MODEL
下载PDF
Determination of trans-resveratrol in mouse liver by high performance liquid chromatography
12
作者 姚倩 侯世祥 +3 位作者 何希辉 张瑄 颜军 苟小军 《Journal of Chinese Pharmaceutical Sciences》 CAS 2008年第2期158-162,共5页
To develop a sensitive high performance liquid chromatography (HPLC) assay for the determination of trans-resveratrol in mouse liver. The whole liver of a mouse was removed from the body, homogenated, and extracted ... To develop a sensitive high performance liquid chromatography (HPLC) assay for the determination of trans-resveratrol in mouse liver. The whole liver of a mouse was removed from the body, homogenated, and extracted by ethyl acetate. The organic layer was isolated and evaporated to dryness, the residue was reconstituted in 0.2 mL mobile phase for centrifugation, and 50 uL of the supernatant was injected into the/-IPLC instrument. The sample was separated on a Shimadzu ODS column (150 mm × 4.6 mm, 5 um) at 35 ℃ and detected by ultraviolet (UV) detector at the wavelength of 305 nm. The mobile phase consisted of methanol and 0.1 mol/L acetic acid (4:6, v/v) with the flow-rote at 1 mL/min. The limit of detection was 3.0 ng/g in liver homogenate with a signal/noise ratio of 3:1. The linear range of the calibration curve was 5.0-120.0 ng/g. The mean recoveries at the concentrations of 6, 10 and 80 ng/g were 102%, 96.0% and 91.5%, respectively. The RSDs for inter- and intra-day assays were less than 5%. Compared with other reported methods, this method was faster and more sensitive. It was also proved to be of good linearity, selectivity, accuracy and precision, and can be efficiently applied to the pharmacoldnetic study of trans-resveratrol in mouse liver. 展开更多
关键词 High performance liquid chromatography TRANS-RESVERATROL mouse liver
下载PDF
Actin Visualization in Living Immature Pollen of Rice Using a GFP-Mouse Talin Fusion Protein
13
作者 徐是雄 王凌健 +2 位作者 丘志平 叶永健 余旭红 《Acta Botanica Sinica》 CSCD 2002年第6期642-648,共7页
Green fluorescent protein (GFP) fused to the F_actin binding domain of mouse talin labels the actin cytoskeleton in the immature pollen of stable transformed rice (Oryza sativa L.) plants. Actin microfilaments could b... Green fluorescent protein (GFP) fused to the F_actin binding domain of mouse talin labels the actin cytoskeleton in the immature pollen of stable transformed rice (Oryza sativa L.) plants. Actin microfilaments could be visualized only in the late_developmental stage of the immature pollen. During this developmental stage, microfilaments, initially composed of very short fibrils, develop into a very complex and novel network that sometimes totally and sometimes partially encloses the vegetative nucleus and the spherical shaped generative cell in the central cytoplasm of the immature pollen. The behavior of the actin microfilamentous structure throughout the late_developmental stage of the immature pollen is extremely dynamic, and the likelihood of this structure in generating forces for vegetative nucleus and generative cell movement in the immature pollen has been discussed. No actin filaments were visualized in the spherical generative cells. 展开更多
关键词 Oryza sativa POLLEN green fluorescent protein (GFP) mouse talin ACTIN MICROFILAMENT
下载PDF
The Establishment and Characterization of a Continuous Cell Line of Mouse Cervical Carcinoma
14
作者 顾蓓 冯海凉 +3 位作者 董继红 张宏 卞晓翠 刘玉琴 《Chinese Journal of Clinical Oncology》 CSCD 2008年第1期44-48,共5页
OBJECTIVE To establish a murine uterine cervical cancer cell line and to define its biological characters. METHODS Transplanted tumor tissue was used for in vitro primary culture of U14 cervical carcinoma cells. After... OBJECTIVE To establish a murine uterine cervical cancer cell line and to define its biological characters. METHODS Transplanted tumor tissue was used for in vitro primary culture of U14 cervical carcinoma cells. After 20 passages, we examined its morphology, chromosomes, tumorigenicity and produced a growth curve. CK was detected by immunohistochemistry, the cell cycle determined by flow cytometry and the metastatic potential assessed in 615 and C57BL/c mice. We also transfected the cells with the pEGFP-N1 plasmid. RESULTS A newly established murine cell line was passaged 50 times over a period of 10 months. The cells grow as a partially suspended culture, and are immunohistochemically CK(+). The cell line is characterized by a hypotetraploid karyotype, a chromosomal number of 64-68 and a doubling time of 21.8 h. Exponential growth occurs by the third and forth day of culture. Cell cycle analysis showed G1 34%, G2 26%, and 40% in the S phase. The tumorigenicity was 100% upon implantation. No mycoplasma contamination was detected. A monoclonal continuous U14-GFP cell strain which was 100% GFP (+) was also produced. CONCLUSION We successfully established a new murine cervical U14 carcinoma cell line and an U14-GFP monoclonal strain. These cell lines are ideal for combined in vivo and in vitro tumor research. 展开更多
关键词 mouse uterine cervical cancer cell line biological properties
下载PDF
Experimental study on gas explosion to kill and injury mouse
15
作者 谭迎新 王海宾 任瑞娥 《Journal of Measurement Science and Instrumentation》 CAS CSCD 2015年第4期322-326,共5页
The killing and injury effects of gas explosion shock wave on mouse in an open space pipeline is tested experimentally. When the methane volume fraction is 10M, the maximum explosion pressure is 0. 264 MPa and the inj... The killing and injury effects of gas explosion shock wave on mouse in an open space pipeline is tested experimentally. When the methane volume fraction is 10M, the maximum explosion pressure is 0. 264 MPa and the injury is the most serious. Specially, some designed obstacles put in the open space pipeline are conducive to producing more stronger gas explosion shock wave. Accordingly, the injury effect of methane explosion on mouse is enhanced under obstacles condition. When the methane volume fraction is 10%, the maximum explosion pressure can reach 0. 298 MPa under obstacles conditiorL It can be concluded that to reduce explosive accident impact, the obstacles in coal mine should be avoided. With the explosions increasing, the death pressure of mouse decreases. 展开更多
关键词 mouse animal injury METHANE gas explosion
下载PDF
Study on the Gene Transcription Level of Hippocampus NGF in C_(57) Mouse Development
16
作者 刘晓军 吴梧桐 +1 位作者 张林元 高向东 《Journal of Chinese Pharmaceutical Sciences》 CAS 2000年第2期108-111,共4页
β-Nerve growth factor (β NGF) mRNA levels in hippocampa from C57BL/6J mice of different ages were compared by semi-quantitative RT-PCR method, taking β-actin gene as an internal control. The levels of 6-and 12-mon... β-Nerve growth factor (β NGF) mRNA levels in hippocampa from C57BL/6J mice of different ages were compared by semi-quantitative RT-PCR method, taking β-actin gene as an internal control. The levels of 6-and 12-month-old C57 mice were much lower compared with that of the l-month-old mouse. However, there was no significant difference between these two groups. This result suggests that gene transcription level of hippocampus β NGF in a C57, mouse descends evidently as it grows up. Base T at Site 294 of β NGF cDNA was substituted by C in the C57, mouse, as revealed by DNA sequencing for the first time. Nevertheless, this polymorphism of nucleotide did not make any difference in amino acid composition. 展开更多
关键词 NGF β-actin RT-PCR Gene transcription C57 mouse Development
下载PDF
Effect of Sex Steroid Hormones on Brain Tubulin Synthesis in Castrated Male Mouse & Testis-intact Male Mouse
17
作者 郭守祥 孙育敏 +3 位作者 李兆菲 张纬 李云生 田德润 《Developmental and Reproductive Biology》 1996年第1期42-48,共7页
The effect of gonadal hormones on brain tubulin synthesis has been studied in the castrated middle-aged male mouse and testes intact oldaged male mouse.Estrogen exerts potent influences on stimulating tubulin synthesi... The effect of gonadal hormones on brain tubulin synthesis has been studied in the castrated middle-aged male mouse and testes intact oldaged male mouse.Estrogen exerts potent influences on stimulating tubulin synthesis.Treatment with estradiol benzoate or estradiol benzoate and hydroxyprogesterone caproate increase the amount of tubulin by almost 37%-43%.Administration of testosterone propionate can produce a 13%-23% increase.Both estrogen and androgen can stimulate tubulin synthesis after the critical period of brain development but, estrogen plays a stronger role than androgen in stimulating tubulin synthesis in male mouse. 展开更多
关键词 TUBULIN Steroid Hormones Estrogen TESTOSTERONE PROGESTERONE mouse
下载PDF
Comparative Study on Activation and Fertilization of Mouse Oocytes at Different Ages
18
作者 曹佐武 钟瑜 +1 位作者 潘善培 刘学高 《Developmental and Reproductive Biology》 1997年第2期33-37,共5页
Comparisons of activation rates and fertilization rates were made among oocytes at different ages. Results showed that oocytes at different ages had different activation and fertilization rates when stimulated by sper... Comparisons of activation rates and fertilization rates were made among oocytes at different ages. Results showed that oocytes at different ages had different activation and fertilization rates when stimulated by sperm or ethanol. Oocytes at 15~24 h after the injection of hCG were readily activated by 8% ethanol. The activation rate of oocytes increased with the age of oocytes, up to the highest average of 81.6%, but decreased after 20 h posthCG. Oocytes at 20 h posthCG exhibited the highest immediate cleavage rate(48.0%) after being stimulated by ethanol. On the other hand, 13~15 h oocytes exhibited higher fertilization rates, and the older oocytes were more difficult to be fertilized by sperm in vitro. These suggest that oocytes can be activated in different ways; the mechanism of fertilization might be different from that of activation; and in vitro fertilization is more dependent on oocyte age. 展开更多
关键词 mouse oocyte in vitro fertilization artificial activation
下载PDF
Chronological and Morphological Progression of Nucleus during Mouse Oocyte Maturation and Fertilization in vitro
19
作者 孙青原 刘灵 +3 位作者 李明文 段崇文 刘辉 陈大元 《Developmental and Reproductive Biology》 1996年第1期24-33,T001-T004,共14页
The chronological and morphological changes of the nucleus during mouse oocyte maturation and fertilization were systematically studied. Although most oocytes went through GVBD 2-4 hrs after culture, 13.6% remained at... The chronological and morphological changes of the nucleus during mouse oocyte maturation and fertilization were systematically studied. Although most oocytes went through GVBD 2-4 hrs after culture, 13.6% remained at GV stage 8 hrs after culture.TEM observation revealed that nucleoli of oocytes which failed to go through GVBD were composed of fibrillar-granular component,small vacuoles and fibrillar centers or showed small vacuoles on nuclear surface. During GVBD, the nucleoli became smaller and smaller and finally disappeared with the nuclear-associated chromatin dislocated to the periphery. Nuclear membrane with attached chromatin became folded and electronic dense cores appeared in the center of chromatin clumps at the same time.The last event of GVBD was the disruption of nuclear membrane.At the end of the 5th hr after culture, meiosis progressed to prometaphase I.Chromosomes,distributed in the original GV area free of organelles,were surrounded by large quantity of mitochondria and small SER vesicles. At the end of the 12th hr after culture,48. 1% of the oocytes emitted PB1.Decondensing sperm head and early male pronuclcus(mPN)with condensed nucleoli were found 1-2 hrs after insemination.The formation and enlargement of female PN(fPN) occurred a little earlier than that of mPN. 33.3% finished syngamy at 8-9 hrs after insemination.The process of nucleolus formation was reverse to that in GVBD. The oolemma modification caused by cortical reaction could effectively inhibit polyspermy.in contrast,there were sperm binding to the oolemma where CGs failed to be released. In addition, PB2 was emitted 2-5 hrs after insemination. The difference between PB1 and PB2 as well as the abstriction of polar body were also discussed. 展开更多
关键词 mouse germinal vesicle breakdown (GVBD) pronucleus formation polar body (PB) NUCLEOLUS
下载PDF
Construction of pINC-lacZ Retroviral Vector and its Expression in Mouse Embryonic Stem Cells
20
作者 何维 吴鹤龄 《Developmental and Reproductive Biology》 1996年第1期1-6,共6页
A retroviral vector pINC-lacZ containing an E coli β-galactosidase(β-gal)gene was constructed and introduced into the MESPU-13 cells by electroporation.Four G418-resistant colonies were obtained from 1×107,elec... A retroviral vector pINC-lacZ containing an E coli β-galactosidase(β-gal)gene was constructed and introduced into the MESPU-13 cells by electroporation.Four G418-resistant colonies were obtained from 1×107,electroporated MESPU-13 cells. Histochemical staining for β-gal activity showed that lacZ gene was expressed in at least three of the four colonies.Southern analysis proved that one copy of foreign gene was integrated in the chromosome of one of the transformed lines(MC15).These results showed that the expression of lacZ gene driven by SiCMVIE promoter can be detected in the transformed MESPU-13 cells. 展开更多
关键词 ELECTROPORATION Transgenic mouse pINC-LacZ Retroviral Vector β-galactosidase gene
下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部