With the development of reform and open policy, foreign languages become more and moreuseful. And how to teach a foreign language is an important matter. A lot of methods of teaching foreign languages have been develo...With the development of reform and open policy, foreign languages become more and moreuseful. And how to teach a foreign language is an important matter. A lot of methods of teaching foreign languages have been developed. There’re grammar-trans-lation method, direct method, audio-lingual method, communicative method, etc. In this paper I in-展开更多
Advantages and disadvantages of four transfection methods of DNA vaccines into the body were introduced in this study, including direct transfection, transfection of bacterial vectors, cationic lipofection and cationi...Advantages and disadvantages of four transfection methods of DNA vaccines into the body were introduced in this study, including direct transfection, transfection of bacterial vectors, cationic lipofection and cationic polymer transfection. Cationic polymer had the characteristics of high transfection efficiency, simple operation, wide application, good repeatability and low cell virulence, which could be expected as a new kind of effi- cient transfection reagents. In practical application, various transfection methods have their own advantages and disadvantages, so the best choice should be made in the design process of vaccines based on targets, test cost, using objects and its convenience.展开更多
文摘With the development of reform and open policy, foreign languages become more and moreuseful. And how to teach a foreign language is an important matter. A lot of methods of teaching foreign languages have been developed. There’re grammar-trans-lation method, direct method, audio-lingual method, communicative method, etc. In this paper I in-
基金supported by Project of Zhejiang Province(2006C12086)
文摘Advantages and disadvantages of four transfection methods of DNA vaccines into the body were introduced in this study, including direct transfection, transfection of bacterial vectors, cationic lipofection and cationic polymer transfection. Cationic polymer had the characteristics of high transfection efficiency, simple operation, wide application, good repeatability and low cell virulence, which could be expected as a new kind of effi- cient transfection reagents. In practical application, various transfection methods have their own advantages and disadvantages, so the best choice should be made in the design process of vaccines based on targets, test cost, using objects and its convenience.
