The study comparatively examined the leaf photosynthetic capacities of different adzuki bean cultivars, high-yield 2000-75 and Jihong 9218, and low-yield Hongbao 1 and Wanxuan 1 from flowering to ripening. It showed t...The study comparatively examined the leaf photosynthetic capacities of different adzuki bean cultivars, high-yield 2000-75 and Jihong 9218, and low-yield Hongbao 1 and Wanxuan 1 from flowering to ripening. It showed that after flowering, the leaves of the cultivars gradually aged, the leaf chlorophyll (Chl.), soluble protein (SP) contents, net photosynthetic rates (Pn), transpiration rates (Tr) and stomatal conductance (G) of the cultivars tended to decline, but the leaf intercellular CO2 concentration (Ci) of the cultivars tended to rise. The leaf photosynthetic capacities of the cultivars decreased gradually from the lower to the upper nodes. The dry seed yields of the cultivars were positively correlated with their leaf Chl., SP, Pn, and Tr and Gs, and negatively associated with their leaf Ci. At the late growth stages, the high-yield cultivars maintained higher leaf Chl. contents, SP contents, Pn, Tr, and Gs than the low-yield cultivars, indicating that leaf photosynthetic capacity was one of important yield-affecting factors of adzuki bean. Therefore, it was important for a crop at the crucial stage of yield formation to maintain a high leaf chlorophyll content and a high leaf photosynthetic capacity and delay leaf aging.展开更多
Due to global climate change,Korea is facing severe droughts that affect the planting and early vegetative periods of upland crops.Soybean and adzuki bean are important legume crops in Korea,so it is critical to under...Due to global climate change,Korea is facing severe droughts that affect the planting and early vegetative periods of upland crops.Soybean and adzuki bean are important legume crops in Korea,so it is critical to understand their adaptations to water stress.This study investigated the changes in root morphological properties in soybean and adzuki bean and quantified the findings using fractal analysis.The experiment was performed at the National Institute of Crop Science in Miryang,Korea.Soybeans and adzuki beans were planted in test boxes and grown for 30 days.The boxes were filled with bed soil with various soil moisture treatments.Root images were obtained and scanned every two days,and the root properties were characterized by root length,depth and surface area,number of roots,and fractal parameters(fractal dimension and lacunarity).Root depth,length and surface area and the number of roots increased in both crops as the soil moisture content increased.The fractal dimension and lacunarity values increased as the soil moisture content increased.These results indicated that the greater the soil moisture,the more heterogeneous the root structure.Correlation analysis of the morphological properties and fractal parameters indicated that soybean and adzuki bean had different root structure developments.Both soybean and adzuki bean were sensitive to the amount of soil moisture in the early vegetative stage.Soybean required a soil moisture content greater than 70%of the field capacity to develop a full root structure,while adzuki bean required 100%of the field capacity.These results would be useful in understanding the responses of soybean and adzuki bean to water stress and managing irrigation during cultivation.展开更多
Genetic diversity of 158 accessions of an applied core collection of adzuki bean (Vigna angularis) and 18 wild genotypes were assessed by using 85 microsatellite markers. With an average of 5.81 alleles per locus, 4...Genetic diversity of 158 accessions of an applied core collection of adzuki bean (Vigna angularis) and 18 wild genotypes were assessed by using 85 microsatellite markers. With an average of 5.81 alleles per locus, 493 alleles were detected, and their distribution frequencies lower than 5% accounted for 73.02% of the total number. The distributions of alleles between the cultivated and the wild adzuki bean germplasm are different, with a higher allelic diversity in the wild germplasm than that of the cultivated ones. An obvious genetic differentiation was also observed between the wild and the cultivated adzuki beans, and SSR markers may be useful in study identification and classification of them. Among cultivated adzuki bean, the genetic similarity coefficient varied from 0.366 to 0.939. Genetic structure analysis can clearly separate the wild genotypes from the cultivated adzuki bean, and also can divide the cultivated ones into different populations, as these populations are closely agreeable with the ecological regions where they originally grow. The results of this study will be useful in arranging local breeding programs, especially in the aspect of parental combinations or identification of progenies. These SSR markers can also provide important information to explain the genetic relationship between the cultivated and wild adzuki beans, and to accelerate the wild gene resources in broadening the gene pool in breeding program.展开更多
This study describes variation of intron-3 of α-amylase gene from 156 breeds of adzuki beans using SSCP(single-strand conformation polymorphism)analysis. Based on α-amylase gene structure and sequence, A pair of P...This study describes variation of intron-3 of α-amylase gene from 156 breeds of adzuki beans using SSCP(single-strand conformation polymorphism)analysis. Based on α-amylase gene structure and sequence, A pair of PCR primers, F (CCTACATTCTAACACACCCT) and R (GCATATTGTGCCAGTACAAT) were designed to amplify intron-3 fragments of α-amylase gene. 14 variant types were detected, including 13, 9, 10, 4 variant types in the wild, weed, locally cultivated and modern brought-up adzuki beans respectively, 9, 8, 7 variant types of the wild adzuki beans from Japan, China and Korea respectively, and some other variant types in the local adzuki beans from China and Bhutan. 60% of subjects of cultivated races were found to be EE type in the experiment. In addition, sequence analysis of intron-3 of α-amylase gene from 8 variant types reveals the evolution process of various variant types in adzuki beans.展开更多
A set of representative 146 adzuki (Vigna angular is var. angularis, and var. nipponensis) germplasm from 6 Asian countries traditionally for adzuki bean production, together with an out group standard rice bean (Vign...A set of representative 146 adzuki (Vigna angular is var. angularis, and var. nipponensis) germplasm from 6 Asian countries traditionally for adzuki bean production, together with an out group standard rice bean (Vigna umbellata), were analyzed by AFLP methodology using 12 informative primer pairs. 313 unambiguous polymorphic bands were created. According to the dendrogram by cluster analysis based on AFLP banding, 143 of the accessions were distinct and revealed enough genetic diversity for identification and classification of accessions within Vigna angularis. A neighbor joining tree was generated using newly developed Innan's nucleotide diversity estimate from the AFLP data. From analysis, 7 distinct evolutionary groups, named as "Chinese cultivated", "Japanese cultivated", "Japanese complex-Korean cultivated", "Chinese wild", "China Taiwan wild", "Nepal-Bhutan cultivated" and "Hymalayan wild", were detected. Nucleotide diversity with geographical distribution of each group is discussed, regarding the evolutionary relationships between wild and cultivated adzuki beans. The preliminary results indicated that cultivated adzuki bean should be domesticated from at least 4 progenitors in at least 3 geographical origins.展开更多
According to the experiment and production practice,this paper brings forward the technical regulations on the production technology,the environmental conditions of production area,the output index,the site selection ...According to the experiment and production practice,this paper brings forward the technical regulations on the production technology,the environmental conditions of production area,the output index,the site selection and land preparation,the variety selection and seed quality,the fertilization,the sowing,the field management,the pest and disease control,the harvesting,the storage,the filing etc. about the adzuki beans in Qingyang City.展开更多
为了研究富含GABA萌发红小豆对2型糖尿病(Diabetes Mellitus Type 2,T2DM)小鼠血糖水平及肠道菌群的影响,采用C57BL/6J小鼠为研究对象,通过高脂膳食+链脲菌素(Streptozocin,STZ)注射构建T2DM模型,选择不同剂量的富含GABA萌发红小豆对T2D...为了研究富含GABA萌发红小豆对2型糖尿病(Diabetes Mellitus Type 2,T2DM)小鼠血糖水平及肠道菌群的影响,采用C57BL/6J小鼠为研究对象,通过高脂膳食+链脲菌素(Streptozocin,STZ)注射构建T2DM模型,选择不同剂量的富含GABA萌发红小豆对T2DM小鼠连续膳食干预6周,并利用16S rRNA测序技术对T2DM小鼠盲肠内容物的菌群结构和分布进行鉴定。结果显示:不同剂量富含GABA红小豆膳食干预可使T2DM小鼠FBG值明显下降,其中高剂量富含GABA红小豆(TF3)组FBG值为8.36±0.78 mmol/L,相比模型组(M)下降54.09%,干预效果最好。此外,TF3膳食可引起T2DM小鼠肠道菌群丰度发生显著(P<0.05)改变,门水平上Firmicutes丰度为35.96%,比M模型组下降53.17%,并可显著上调Bacteroidetes、Verrucomicrobia的菌群丰度(P<0.05)。表明TF3膳食改善糖脂代谢与Bacteroidetes、Verrucomicrobia优势菌丰度呈正相关,暗示高剂量富含GABA红小豆膳食可通过增加有益菌来缓解T2DM小鼠高血糖症状,为进一步解释T2DM与肠道菌群的关系提供参考。展开更多
In this study,adzuki bean peptides were prepared by semi-solid enzymatic hydrolysis(SEH)and their antioxidant and Angiotensin-I-converting enzyme(ACE)inhibitory activities and internal absorption were studied.By modif...In this study,adzuki bean peptides were prepared by semi-solid enzymatic hydrolysis(SEH)and their antioxidant and Angiotensin-I-converting enzyme(ACE)inhibitory activities and internal absorption were studied.By modifying different enzymatic hydrolysis times and enzyme additions,the optimal enzymatic hydrolysis condition for SEH could be obtained as adzuki bean powder:water=4:1(w/w),alcalase:neutrase=2:1(w/w),enzyme addition 3.75%(w/w),4 h enzymatic hydrolysis at 50℃ and pH=8.Within these hydrolysis conditions,the content of adzuki bean peptides was 11.37%,the OH−scavenging rate of 1 mg/mL peptides was 72.75%,the total antioxidant capacity was 0.88±0.02 mmol/L FeSO4,the ACE inhibition rate was 77.49%.In vivo absorption test proved that the peptides prepared by SEH were absorbed faster and exhibited higher antioxidant activity compared with liquid enzymatic hydrolysis.In conclusion,the process of SEH can improve not only the efficiency of hydrolysis,but also the antioxidant and ACE inhibitory activity as well as the internal absorption of adzuki bean peptides.展开更多
To produce high sensory quality sweetened adzuki an,the effects of cooking conditions including cooking time,heating power,sugar soaking time and soaking liquid pH on textural properties and sensory scores were evalua...To produce high sensory quality sweetened adzuki an,the effects of cooking conditions including cooking time,heating power,sugar soaking time and soaking liquid pH on textural properties and sensory scores were evaluated using central composite experimental method.Blanching treatment and sodium tripolyphosphate were adopted to improve the mouthfeel of the whole bean an product.Results showed that the optimal parameters were as follows:cooking time of 50 min,heating power of 1.1 kW,sugar soaking time of 2 h and soaking liquid pH of 8.0,which resulted in the highest sensory score of 89.6.In this study,the effectiveness of the method to process sweetened whole bean adzuki an was validated and a sensory evaluation method for whole grain adzuki an product was developed.展开更多
Objective:To enhance physiological activities of adzuki bean(Vigna angularis)via fermentation with Monascus pilosus(M.pilosus).Methods:The adzuki bean fermentation conditions with M.pilosus were optimized,and the effe...Objective:To enhance physiological activities of adzuki bean(Vigna angularis)via fermentation with Monascus pilosus(M.pilosus).Methods:The adzuki bean fermentation conditions with M.pilosus were optimized,and the effect of Monascus-fermentation on the antioxidant capacity and phenolic acid contents of adzuki bean was investigated.Results:Optimal fermentation conditions were determined by the production of monacolin K.The highest monacolin K production was observed in 5%inoculum sized on day 15 in fermentation.Free and bound phenolic acids were isolated from native and fermented adzuki bean.A 1.9-fold decrease was observed in bound p-coumaric acid content,whereas the contents of bound ferulic and sinapic acids were increased by 28-and 1.7-fold,respectively.However,the contents of free phenolic acids such as p-coumaric,ferulic,and sinapic acids were increased by 2.6-,5.2-,and 7.2-fold,respectively.The fermentation of adzuki bean by M.pilosus enhanced the activities of DPPH■radical scavenging,ferrous ion-chelating,nitric oxide scavenging,and ferric antioxidant reducing activities 2.2-,1.7-,1.2-,and 1.8-fold,respectively.Conclusions:Results from our study suggest that the contents of p-coumaric,ferulic,and sinapic acids in adzuki bean were highly increased by fermentation with M.pilosus,resulting in enhanced various antioxidant activities.展开更多
基金the Special Fund for Agro-Scientific Research in the Public Interest, China (200903007)the Cyrus Tang Specific Plant Genetics and Breeding Program of the Northwest A&F University, China (No. 50)
文摘The study comparatively examined the leaf photosynthetic capacities of different adzuki bean cultivars, high-yield 2000-75 and Jihong 9218, and low-yield Hongbao 1 and Wanxuan 1 from flowering to ripening. It showed that after flowering, the leaves of the cultivars gradually aged, the leaf chlorophyll (Chl.), soluble protein (SP) contents, net photosynthetic rates (Pn), transpiration rates (Tr) and stomatal conductance (G) of the cultivars tended to decline, but the leaf intercellular CO2 concentration (Ci) of the cultivars tended to rise. The leaf photosynthetic capacities of the cultivars decreased gradually from the lower to the upper nodes. The dry seed yields of the cultivars were positively correlated with their leaf Chl., SP, Pn, and Tr and Gs, and negatively associated with their leaf Ci. At the late growth stages, the high-yield cultivars maintained higher leaf Chl. contents, SP contents, Pn, Tr, and Gs than the low-yield cultivars, indicating that leaf photosynthetic capacity was one of important yield-affecting factors of adzuki bean. Therefore, it was important for a crop at the crucial stage of yield formation to maintain a high leaf chlorophyll content and a high leaf photosynthetic capacity and delay leaf aging.
基金This research was performed and funded by an Agenda Project of the Rural Development Administration(PJ 013482022020),Republic of Korea.
文摘Due to global climate change,Korea is facing severe droughts that affect the planting and early vegetative periods of upland crops.Soybean and adzuki bean are important legume crops in Korea,so it is critical to understand their adaptations to water stress.This study investigated the changes in root morphological properties in soybean and adzuki bean and quantified the findings using fractal analysis.The experiment was performed at the National Institute of Crop Science in Miryang,Korea.Soybeans and adzuki beans were planted in test boxes and grown for 30 days.The boxes were filled with bed soil with various soil moisture treatments.Root images were obtained and scanned every two days,and the root properties were characterized by root length,depth and surface area,number of roots,and fractal parameters(fractal dimension and lacunarity).Root depth,length and surface area and the number of roots increased in both crops as the soil moisture content increased.The fractal dimension and lacunarity values increased as the soil moisture content increased.These results indicated that the greater the soil moisture,the more heterogeneous the root structure.Correlation analysis of the morphological properties and fractal parameters indicated that soybean and adzuki bean had different root structure developments.Both soybean and adzuki bean were sensitive to the amount of soil moisture in the early vegetative stage.Soybean required a soil moisture content greater than 70%of the field capacity to develop a full root structure,while adzuki bean required 100%of the field capacity.These results would be useful in understanding the responses of soybean and adzuki bean to water stress and managing irrigation during cultivation.
基金supported by the Earmarked Fund for China Agriculture Research System (CARS-09)
文摘Genetic diversity of 158 accessions of an applied core collection of adzuki bean (Vigna angularis) and 18 wild genotypes were assessed by using 85 microsatellite markers. With an average of 5.81 alleles per locus, 493 alleles were detected, and their distribution frequencies lower than 5% accounted for 73.02% of the total number. The distributions of alleles between the cultivated and the wild adzuki bean germplasm are different, with a higher allelic diversity in the wild germplasm than that of the cultivated ones. An obvious genetic differentiation was also observed between the wild and the cultivated adzuki beans, and SSR markers may be useful in study identification and classification of them. Among cultivated adzuki bean, the genetic similarity coefficient varied from 0.366 to 0.939. Genetic structure analysis can clearly separate the wild genotypes from the cultivated adzuki bean, and also can divide the cultivated ones into different populations, as these populations are closely agreeable with the ecological regions where they originally grow. The results of this study will be useful in arranging local breeding programs, especially in the aspect of parental combinations or identification of progenies. These SSR markers can also provide important information to explain the genetic relationship between the cultivated and wild adzuki beans, and to accelerate the wild gene resources in broadening the gene pool in breeding program.
文摘This study describes variation of intron-3 of α-amylase gene from 156 breeds of adzuki beans using SSCP(single-strand conformation polymorphism)analysis. Based on α-amylase gene structure and sequence, A pair of PCR primers, F (CCTACATTCTAACACACCCT) and R (GCATATTGTGCCAGTACAAT) were designed to amplify intron-3 fragments of α-amylase gene. 14 variant types were detected, including 13, 9, 10, 4 variant types in the wild, weed, locally cultivated and modern brought-up adzuki beans respectively, 9, 8, 7 variant types of the wild adzuki beans from Japan, China and Korea respectively, and some other variant types in the local adzuki beans from China and Bhutan. 60% of subjects of cultivated races were found to be EE type in the experiment. In addition, sequence analysis of intron-3 of α-amylase gene from 8 variant types reveals the evolution process of various variant types in adzuki beans.
文摘A set of representative 146 adzuki (Vigna angular is var. angularis, and var. nipponensis) germplasm from 6 Asian countries traditionally for adzuki bean production, together with an out group standard rice bean (Vigna umbellata), were analyzed by AFLP methodology using 12 informative primer pairs. 313 unambiguous polymorphic bands were created. According to the dendrogram by cluster analysis based on AFLP banding, 143 of the accessions were distinct and revealed enough genetic diversity for identification and classification of accessions within Vigna angularis. A neighbor joining tree was generated using newly developed Innan's nucleotide diversity estimate from the AFLP data. From analysis, 7 distinct evolutionary groups, named as "Chinese cultivated", "Japanese cultivated", "Japanese complex-Korean cultivated", "Chinese wild", "China Taiwan wild", "Nepal-Bhutan cultivated" and "Hymalayan wild", were detected. Nucleotide diversity with geographical distribution of each group is discussed, regarding the evolutionary relationships between wild and cultivated adzuki beans. The preliminary results indicated that cultivated adzuki bean should be domesticated from at least 4 progenitors in at least 3 geographical origins.
基金Supported by the First Batch of Planned Revised Local Standards of Gansu Provincial Bureau of Quality Supervision,Inspection and Quarantine of the People's Republic of China in 2016(2016-T-015)
文摘According to the experiment and production practice,this paper brings forward the technical regulations on the production technology,the environmental conditions of production area,the output index,the site selection and land preparation,the variety selection and seed quality,the fertilization,the sowing,the field management,the pest and disease control,the harvesting,the storage,the filing etc. about the adzuki beans in Qingyang City.
基金supported by the National Key Research and Development Program of China[2021YFC2101400].
文摘In this study,adzuki bean peptides were prepared by semi-solid enzymatic hydrolysis(SEH)and their antioxidant and Angiotensin-I-converting enzyme(ACE)inhibitory activities and internal absorption were studied.By modifying different enzymatic hydrolysis times and enzyme additions,the optimal enzymatic hydrolysis condition for SEH could be obtained as adzuki bean powder:water=4:1(w/w),alcalase:neutrase=2:1(w/w),enzyme addition 3.75%(w/w),4 h enzymatic hydrolysis at 50℃ and pH=8.Within these hydrolysis conditions,the content of adzuki bean peptides was 11.37%,the OH−scavenging rate of 1 mg/mL peptides was 72.75%,the total antioxidant capacity was 0.88±0.02 mmol/L FeSO4,the ACE inhibition rate was 77.49%.In vivo absorption test proved that the peptides prepared by SEH were absorbed faster and exhibited higher antioxidant activity compared with liquid enzymatic hydrolysis.In conclusion,the process of SEH can improve not only the efficiency of hydrolysis,but also the antioxidant and ACE inhibitory activity as well as the internal absorption of adzuki bean peptides.
基金financially supported in part by the National“948”project of Ministry of Agriculture,P.R.China.
文摘To produce high sensory quality sweetened adzuki an,the effects of cooking conditions including cooking time,heating power,sugar soaking time and soaking liquid pH on textural properties and sensory scores were evaluated using central composite experimental method.Blanching treatment and sodium tripolyphosphate were adopted to improve the mouthfeel of the whole bean an product.Results showed that the optimal parameters were as follows:cooking time of 50 min,heating power of 1.1 kW,sugar soaking time of 2 h and soaking liquid pH of 8.0,which resulted in the highest sensory score of 89.6.In this study,the effectiveness of the method to process sweetened whole bean adzuki an was validated and a sensory evaluation method for whole grain adzuki an product was developed.
基金Supported by a grant from the Next-Generation BioGreen 21 Program(No.PJ009517),Rural Development Administration,Republic of Korea.
文摘Objective:To enhance physiological activities of adzuki bean(Vigna angularis)via fermentation with Monascus pilosus(M.pilosus).Methods:The adzuki bean fermentation conditions with M.pilosus were optimized,and the effect of Monascus-fermentation on the antioxidant capacity and phenolic acid contents of adzuki bean was investigated.Results:Optimal fermentation conditions were determined by the production of monacolin K.The highest monacolin K production was observed in 5%inoculum sized on day 15 in fermentation.Free and bound phenolic acids were isolated from native and fermented adzuki bean.A 1.9-fold decrease was observed in bound p-coumaric acid content,whereas the contents of bound ferulic and sinapic acids were increased by 28-and 1.7-fold,respectively.However,the contents of free phenolic acids such as p-coumaric,ferulic,and sinapic acids were increased by 2.6-,5.2-,and 7.2-fold,respectively.The fermentation of adzuki bean by M.pilosus enhanced the activities of DPPH■radical scavenging,ferrous ion-chelating,nitric oxide scavenging,and ferric antioxidant reducing activities 2.2-,1.7-,1.2-,and 1.8-fold,respectively.Conclusions:Results from our study suggest that the contents of p-coumaric,ferulic,and sinapic acids in adzuki bean were highly increased by fermentation with M.pilosus,resulting in enhanced various antioxidant activities.