秀珍菇菌种复壮方法比较

以从秀珍菇(Pleurotus geesteranus)污染菌包中分离得到的3个秀珍菇菌株为出发菌株,采用挑取尖端菌丝、基内菌丝及气生菌丝3种方法进行复壮处理,共获得处理菌株8个。以出发菌株为对照,比较处理菌株的菌落形态、菌丝生长速度及菌丝生物量,并通过出菇实验以及dsRNA检测,对不同复壮方法的复壮效果进行评价。结果表明:尖端菌丝和基内菌丝挑取法能显著提高出发菌株的菌丝生长速度和生物量,可以缩短出菇周期并提高出菇产量;而气生菌丝挑取法则不能对出发菌株进行复壮,其菌丝生物量和出菇产量反而比出发菌株有所降低。

羊肚菌菌丝体生长特性及菌核培养特征的初步观察

对两种野生羊肚菌菌株M1和M2在不同培养基上菌丝生长及菌核产生的特征进行了初步观察。结果表明，M1与M2相比，前者菌丝的生长势强；VB1可促进菌丝体和菌核的生长；

培养基基质对羊肚菌菌丝体和菌核生长特性的影响

菌丝体和菌核的产生是羊肚菌子实体形成的重要阶段,而不同的培养基对菌丝和菌核产生的时间和数量有明显的影响。本文主要对两种野生羊肚菌菌株在不同培养基上菌丝生长及菌核产生的特征进行了研究。结果表明,M1在菌丝生长和菌核形成方面都优于M2,菌丝生长势强、菌核密度大;⑥号培养基在菌丝生长和菌核形成方面表现最好,其次是④号培养基,⑤号培养基表现最差。

黑木耳基内、外菌丝生长一致性研究

本文通过对黑木耳菌丝在c PDA培养基质内、外菌丝生长速度一致性的研究,探索培养基气生菌丝与基内菌丝生长状况,考察其生长的均一性、一致性问题。结果表明,c PDA培养基质中气生菌丝生长速度和基内菌丝生长速度高度一致,不同生长圈气生、基内菌丝生长也高度一致,仅在回接时菌丝萌发和生长速度上存在差异性。

Unexpected Properties of Micromonosporae from Marine Origin

Members of the genus Micromonospora show a complex life cycle which normally involves the presence of substrate or vegetative mycelia and sporulation with single spores born on the vegetative hyphae followed by the synthesis of a dark extracellular polysaccharide. Bergey’s Manual states that micromonosporae rarely produces aerial mycelia (AM) and if so, is considered “sterile”. During the characterisation of novel micromonosporae from the Sea of Cortes, it was observed that AM is produced reproducibly in the presence of certain carbon and/or nitrogen sources. Micromanipulation of the AM subcultured onto fresh media produced colonies;hence, this structure should not be called “sterile”. TEM of the AM producing isolates suggests that the spores also show activity as reported for bacilli of marine origin. This would be the first report of the presence of “inducible” AM in micromonosporae of marine sources and that the spores of this genus have a role other than just dispersal.

灵芝液体浅层静置培养高效生产三萜的研究

三萜是灵芝Ganoderma lingzhi中重要的活性物质。本研究采用液体浅层静置培养方式(LSSC)提高灵芝三萜的产量。结果表明,在T25细胞培养瓶中的最佳培养条件为初始培养体积2mL,接种量7.0g(菌体湿重/L),在48h补加2mL培养液,发酵7d,三萜产量可达(32.95±0.51)mg/g,为摇瓶培养最高产量的2.09倍。进一步经不锈钢平盘放大研究发现,三萜最高产量为(65.91±0.84)mg/g,为摇瓶培养的4.19倍。形态学研究表明,浅层静置培养过程中形成的气生菌丝是提高三萜产量的关键因素。与深层液体发酵相比,液体浅层静置培养具有生长快,三萜产量高,发酵过程不需要搅拌等优点,具有良好的工业化应用前景。