The aim of this study was to evaluate the factors influencing the inactivation effect of intense pulsed light(IPL)on Aeromonas salmonicida grown on chicken meat and skin,and to further develop prediction models of ina...The aim of this study was to evaluate the factors influencing the inactivation effect of intense pulsed light(IPL)on Aeromonas salmonicida grown on chicken meat and skin,and to further develop prediction models of inactivation.In this work,chicken meat and skin inoculated with meat-borne A.salmonicida isolates were subjected to IPL treatments under different conditions.The results showed that IPL had obvious bactericidal effect in the chicken skin and thickness groups when the treatment voltage and time were 7 V combined with 5 s.In addition,the lethality curves of A.salmonicida were fitted under IPL conditions of 3.5-7.5 V.The comparison of statistical parameters revealed that the Weibull model could best fit the mortality curves and could accurately predict the mortality dynamic of A.salmonicida grown on chicken skin.And further a secondary model between the scale factor b and the treatment voltage in Weibull model was established using linear equations,which determined that the secondary model could accurately predict the inactivation of A.salmonicida.This study provides a theoretical basis for future prediction models of Aeromonas,and also provides new ideas for sterilization approaches of meat-borne Aeromonas.展开更多
Vibrio scophthalmi and Aeromonas salmonicida can cause high turbot mortality and huge economic losses.Presently,vaccination is the most promising method for preventing communicable diseases.In this study,we used forma...Vibrio scophthalmi and Aeromonas salmonicida can cause high turbot mortality and huge economic losses.Presently,vaccination is the most promising method for preventing communicable diseases.In this study,we used formalin to kill V.scophthalmi and A.salmonicida cells,and mixed with the mineralized oil adjuvant(Montanide^(TM)ISA 763 AVG)to prepare the bivalent inactivated vaccine.The results showed that turbot inoculated with the bivalent inactivated vaccine exhibited strong tolerance to the infection of V.scophthalmi and A.salmonicida,and no obvious clinical symptoms and pathological changes were observed.The activities of enzymes lysozyme,acid phosphatase and complement C3 had significantly increased after the vaccination.The antibody titer response of vaccinated turbot was greatly boosted,which was positively connected with the immunological impact according to ELISA results.Simultaneously,the expression levels of immune-related genes such as MHC-IIα,MHC-IIβ,CD4,CD8,TNF-αand IL^(-1)βwere up-regulated,demonstrating that it might stimulate humoral and cellular immunological response in turbot.These findings highlight the potential of the bivalent inactivated vaccine for controlling V.scophthalmi and A.salmonicida infections in turbot.展开更多
In this study,bacterial pathogens were isolated from the liver,spleen,kidney,heart,and muscle of diseased goldfish(Carassius auratus)after a massive outbreak of disease in an aquaculture farm in Anshan,Liaoning Provin...In this study,bacterial pathogens were isolated from the liver,spleen,kidney,heart,and muscle of diseased goldfish(Carassius auratus)after a massive outbreak of disease in an aquaculture farm in Anshan,Liaoning Province,North China(N41°05′58.42″E122°53′31.89″).Based on physiological and biochemical characterization of the bacteria and 16S rDNA sequence analysis,the bacterial isolates were identified as Aeromonas salmonicida salmonicida.Virulence gene multilocus sequence typing analysis showed that the bacterial isolates from all the organs collected were of the same type,and clustered in the same phylogenetic group as A.salmonicida salmonicida.The pathogen,was named AS.17 and was resistant to rifampicin,bacitracin,vancomycin,penicillin and compound sulfamethoxazole,but was highly sensitive to nalidixic acid,lomefloxacin,spectinomycin,ofloxacin,and ciprofloxacin.Goldfish experimentally infected with AS.17 had similar symptoms to naturally infected fish,including gill filament anemia,intestinal bleeding,intra-abdominal fluid,and jaw bleeding.Histological analyses showed that the liver,the spleen,the kidney and the intestines of fish infected with AS.17 had severe pathological alterations.In summary,this study reports the isolation of A.salmonicida salmonicida from C.auratus in North China and provides a reference strain and a research foundation for further studies on A.salmonicida disease control and epidemiology.展开更多
基金supported by projects funded by grants from the Natural Science Foundation of Jiangsu Province in China(BK20221515)the National Natural Science Foundation of China(32172266)the Changzhou Science and Technology Support Program(CE20222002)。
文摘The aim of this study was to evaluate the factors influencing the inactivation effect of intense pulsed light(IPL)on Aeromonas salmonicida grown on chicken meat and skin,and to further develop prediction models of inactivation.In this work,chicken meat and skin inoculated with meat-borne A.salmonicida isolates were subjected to IPL treatments under different conditions.The results showed that IPL had obvious bactericidal effect in the chicken skin and thickness groups when the treatment voltage and time were 7 V combined with 5 s.In addition,the lethality curves of A.salmonicida were fitted under IPL conditions of 3.5-7.5 V.The comparison of statistical parameters revealed that the Weibull model could best fit the mortality curves and could accurately predict the mortality dynamic of A.salmonicida grown on chicken skin.And further a secondary model between the scale factor b and the treatment voltage in Weibull model was established using linear equations,which determined that the secondary model could accurately predict the inactivation of A.salmonicida.This study provides a theoretical basis for future prediction models of Aeromonas,and also provides new ideas for sterilization approaches of meat-borne Aeromonas.
基金supported by the Fish Innovation Team of Shandong Agriculture Research System (No. SDAIT-1206)the Aquatic Animal Immunologic Agents Engineering Research Center of Shandong Province, the Qingdao Agricultural University Doctoral Start-Up Fund (6631122030)+5 种基金the National Natural Science Foundation of China (No. 32002421)the Advanced Talents Foundation of QAU (No. 6651118016)the Natural Science Foundation of Shandong Province (No. ZR2019BC009)the ‘First-Class Fishery Discipline’ program of Shandong Province, the special top talent plan ‘One Thing One Decision (Yi Shi Yi Yi)’the Key Research and Development Program in Shandong Province (No. 2018YFJH0703)Breeding Plan of Shandong Provincial Qingchuang Research Team (2019)
文摘Vibrio scophthalmi and Aeromonas salmonicida can cause high turbot mortality and huge economic losses.Presently,vaccination is the most promising method for preventing communicable diseases.In this study,we used formalin to kill V.scophthalmi and A.salmonicida cells,and mixed with the mineralized oil adjuvant(Montanide^(TM)ISA 763 AVG)to prepare the bivalent inactivated vaccine.The results showed that turbot inoculated with the bivalent inactivated vaccine exhibited strong tolerance to the infection of V.scophthalmi and A.salmonicida,and no obvious clinical symptoms and pathological changes were observed.The activities of enzymes lysozyme,acid phosphatase and complement C3 had significantly increased after the vaccination.The antibody titer response of vaccinated turbot was greatly boosted,which was positively connected with the immunological impact according to ELISA results.Simultaneously,the expression levels of immune-related genes such as MHC-IIα,MHC-IIβ,CD4,CD8,TNF-αand IL^(-1)βwere up-regulated,demonstrating that it might stimulate humoral and cellular immunological response in turbot.These findings highlight the potential of the bivalent inactivated vaccine for controlling V.scophthalmi and A.salmonicida infections in turbot.
基金funded in part by National Natural Science Foundation of China(41706177)Key Laboratory of Mariculture and Stock Enhancement in North China's Sea Open Project(2018-KF-17)Doctoral Research Project of Dalian Ocean University(HDYJ201617)to Dr.R.J.Li.
文摘In this study,bacterial pathogens were isolated from the liver,spleen,kidney,heart,and muscle of diseased goldfish(Carassius auratus)after a massive outbreak of disease in an aquaculture farm in Anshan,Liaoning Province,North China(N41°05′58.42″E122°53′31.89″).Based on physiological and biochemical characterization of the bacteria and 16S rDNA sequence analysis,the bacterial isolates were identified as Aeromonas salmonicida salmonicida.Virulence gene multilocus sequence typing analysis showed that the bacterial isolates from all the organs collected were of the same type,and clustered in the same phylogenetic group as A.salmonicida salmonicida.The pathogen,was named AS.17 and was resistant to rifampicin,bacitracin,vancomycin,penicillin and compound sulfamethoxazole,but was highly sensitive to nalidixic acid,lomefloxacin,spectinomycin,ofloxacin,and ciprofloxacin.Goldfish experimentally infected with AS.17 had similar symptoms to naturally infected fish,including gill filament anemia,intestinal bleeding,intra-abdominal fluid,and jaw bleeding.Histological analyses showed that the liver,the spleen,the kidney and the intestines of fish infected with AS.17 had severe pathological alterations.In summary,this study reports the isolation of A.salmonicida salmonicida from C.auratus in North China and provides a reference strain and a research foundation for further studies on A.salmonicida disease control and epidemiology.
