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基于DNA模板合成的银簇建立“点亮型”荧光法检测卡那霉素 被引量:1
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作者 林碧霞 蒙佩怡 +5 位作者 陈梦玲 吴峻宇 冷江东 黄月梅 杨诗意 俞英 《分析测试学报》 CAS CSCD 北大核心 2019年第9期1051-1058,共8页
以DNA为模板合成弱荧光的银纳米簇。利用DNA模板中的适配体序列识别目标卡那霉素后,形成发夹结构使适配体序列上的G碱基和C碱基靠近银簇,导致银簇荧光增强。同时,DNA二级结构的改变使银纳米粒子形成小的银纳米簇也促使银簇的荧光增强。... 以DNA为模板合成弱荧光的银纳米簇。利用DNA模板中的适配体序列识别目标卡那霉素后,形成发夹结构使适配体序列上的G碱基和C碱基靠近银簇,导致银簇荧光增强。同时,DNA二级结构的改变使银纳米粒子形成小的银纳米簇也促使银簇的荧光增强。据此建立“点亮型”荧光法对卡那霉素进行定量分析。银簇荧光增强比值与卡那霉素浓度在0.075~8.0μmol/L范围内呈线性关系,线性方程为F/F0=0.616 8C+0.927 6,检出限为36 nmol/L。卡那霉素在牛奶和蜂蜜样品中的回收率为92.4%~112%。此外,随着卡那霉素加入浓度的增加,银簇的橙色荧光逐渐加深,根据荧光颜色深度可对卡那霉素进行可视化半定量检测。该方法具有灵敏度高、选择性好的优点,可用于复杂食品样品中痕量卡那霉素的定量检测。 展开更多
关键词 DNA模板 银纳米簇(agncs) 卡那霉素 可视化检测
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导航与控制装置
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《全球定位系统》 2003年第4期49-49,共1页
关键词 AGNC公司 UNCUN1 导航与控制装置 GPS芯片
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银纳米簇荧光探针检测L-半胱氨酸 被引量:5
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作者 冯建军 张国 +2 位作者 柴瑞涛 朱维晃 康倩文 《分析试验室》 CAS CSCD 北大核心 2020年第1期48-52,共5页
常温下合成了二氢硫辛酸(DHLA)包裹的银纳米簇(AgNCs),并基于L-半胱氨酸对AgNCs的荧光猝灭现象构建了AgNCs荧光探针对Cys的检测方法。结果表明,在优化条件下,AgNCs的荧光猝灭程度和Cys浓度在2.0~100μmol/L范围内呈现良好的线性关系(R2=... 常温下合成了二氢硫辛酸(DHLA)包裹的银纳米簇(AgNCs),并基于L-半胱氨酸对AgNCs的荧光猝灭现象构建了AgNCs荧光探针对Cys的检测方法。结果表明,在优化条件下,AgNCs的荧光猝灭程度和Cys浓度在2.0~100μmol/L范围内呈现良好的线性关系(R2=0.998),检出限为1.77μmol/L(S/N=3)。在人体血清样品中Cys检测的加标回收率为94.0%~102.4%。 展开更多
关键词 L-半胱氨酸 二氢硫辛酸(DHLA) 银纳米簇(agncs) 荧光探针 荧光猝灭
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Novel dual fluorescence temperature-sensitive chameleon DNA-templated silver nanocluster pair for intracellular thermometry 被引量:6
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作者 Weijun Zhou Jinbo Zhu +3 位作者 Ye Teng Baoji Du Xu Han Shaojun Dong 《Nano Research》 SCIE EI CAS CSCD 2018年第4期2012-2023,共12页
For the first time, we are reporting a novel type of dual fluorescence temperaturesensitive DNA-templated silver nanocluster (AgNC) pair, which contains two pieces of single-stranded AgNC in proximity through hybrid... For the first time, we are reporting a novel type of dual fluorescence temperaturesensitive DNA-templated silver nanocluster (AgNC) pair, which contains two pieces of single-stranded AgNC in proximity through hybridization. Both the chameleon AgNC pairs, A-NCP and B-NCP, possess two bright fluorescence peaks that achieve sensitive variations corresponding to temperature change from 15 to 45 ℃. With the increase in temperature, one of the fluorescence emissions of A-NCP (A-FL570) increases, while the other (A-FL640) decreases. However, both the emissions of B-NCP (B-FL685 and B-FL620) decrease simultaneously. Therefore, A-NCP shows a remarkable fluorescence color variation from orange to yellow, while the fluorescence color of B-NCP changes from orange to colorless, with increase in temperature. Moreover, the temperature responding linear range of A-NCP can be regulated by adjusting the structures and sequences of assistant DNA templates. It is assumed that the two single-stranded segmental AgNCs are integrated together as they are assembled into AgNC pairs, leading to a dramatic variation in fluorescence properties. The temperature-sensitive phenomenon is due to the dehybridization-induced separation of two pieces of segmental AgNC, caused by temperature increase. The temperature-sensitive AgNC pairs have been successful in indicating the temperature of living cells, showing the potential for a new application of silver nanocluster as a nanothermometer with adjustable response range, bringing novel insight into the regulatory mechanism of AgNC fluorescence variation. 展开更多
关键词 DNA-templated silver nanocluster (AgNC) CHAMELEON TEMPERATURE-SENSITIVE fluorescence
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