藿香植物组织培养技术研究

选用藿香健壮枝条和叶片作为试验材料，进行组培技术的研究。结果表明：藿香取材季节以夏季较好，适宜的外植体种类为茎段。诱导愈伤组织形成的激素组合为6-BA1．0mg／L＋NAA0．3mg／L＋2，4-D0．3mg／L，具有愈伤组织生长速度快并易于分化；不定芽分化激素组合为6-BA0．5mg／L＋NAA0．1mg／L，继代培养组合6-BA0．1mg／L＋NAA0．5mg／L＋B9 0．3mg／L，增殖在8倍以上；生根培养组合1／2MS＋NAA0．6mg／L，一般20d左右生根，平均根量4～5条，生根率达92％。

广藿香与土藿香的DNA序列分析及其分子鉴别

AIM To analyze sequences of the nuclear ribosomal RNA small subunit (18S rRNA) gene and the chloroplast mat K gene of crude drug Patchouli ( Pogostemon cablin ) in order to provide molecular evidence for identification of Patchouli drug. METHODS To sequence the entire 18S rRNA gene and partial mat K gene of Patchouli from Guangzhou and its substitute Wrinkled Gianthyssop ( Agastache rugosa ) from S ichuan using PCR direct sequencing and to detect the homology of two gene sequen ces between these two crude drugs. RESULTS The complete 18S rRNA gene sequence is 1 805 bp in length for Patchouli from Guangzhou whereas 1 794 bp for Wrinkled Gianthyssop from Sichuan. The 3′ end sequence of mat K gene is 521 bp (747～ 1 268 nt from upstream of ma t K gene) for these two crude drugs. Based on multiple sequence alignment, it i s found that there are 18 variable sites and 11 aligned gap sites in 18S rRNA se quence, 49 variable sites in 3′ mat K sequence between these two crude drug s. The homology is 98 4% for 18S rRNA and 90 6% for 3′ mat K between two crude drugs, respectively. CONCLUSION DNA sequencing can provide an accurate and reliable tool in the crude drug ident ification of Patchouli and its substitute Wrinkled Gianthyssop.

藿香中一种新的二萜类物质的分离及其结构表征

[目的]对秦巴山区野生藿香枝叶中萜类物质进行了提取和分离,并对其萜类物质的结构进行了研究。[方法]用浓度95%的乙醇对藿香枝叶粉末进行浸提,并将所得浸膏分散在水相中,依次用石油醚、乙酸乙酯和正丁醇进行萃取,将所得的乙酸乙酯萃取物用硅胶柱层析梯度洗脱法分离其中的萜类物质;运用薄层色谱、红外光谱、紫外光谱、核磁共振谱等现代光谱技术对所得的萜类物质结构进行表征,并采用单晶X-衍射技术确定其晶体分子的构型和构象。[结果]从藿香中分离得到一种新的化合物。该二萜类物质是Longikaurin D,其晶体属于orthorhombic晶系,P2(1)2(1)2(1)空间群,晶胞参数a=0.845 1(4)nm,b=1.061 5(5)nm,c=2.237 1(10)nm,α=β=γ=90.00°,Mr=404.43,V=2.007(16)nm3,Dc=1.352 mg/m3,Z=4,F(000)=872,μ=0.103/mm,R1=0.076 7,ωR2=0.146 8。[结论]首次从在藿香中分离出二萜类物质Longikaurin D,为开发和利用藿香中的萜类物质提供科学依据。

大黄、藿香中草药液诱杀金龟子雌虫效果试验

选用大黄（Rheum palmatum L.）、藿香[Agastache rugosa（Fisch.et Mey.）O.Ktze.]中草药熬制液,利用芳香气味和促进大量排泄的特性,引诱喂养金龟子（Scarabaeoidae）成虫,破坏雌虫产卵排泄系统,减少后代繁育,降低虫口基数,为无公害防治提供技术参考。