<b><span>Background:</span></b><span> The patients’ age is considered a modified risk factor that causes periodontal disease, dental caries, and their development.</span><b...<b><span>Background:</span></b><span> The patients’ age is considered a modified risk factor that causes periodontal disease, dental caries, and their development.</span><b><span> Objective:</span></b><span> The present study was designed to evaluate the impact of salivary pH value and dental caries index on periodontal status among adult age groups of patients from Aseer region, Saudi Arabia</span><span>.</span><span> </span><b><span>Methods:</span></b><span> A cross-sectional analysi</span><span>s carried out in the college of dentistry, King Khalid University on 750 </span><span>adult patients (400 males and 350 females). The adults patients were divided into three equal groups according to the patients’ age: group I (20 - 30 years), group I</span><span>I (31 - 40 years), and group III (41 - 50 years).</span><span> </span><span>Plaque index (PLI), gingi</span><span>val index (GI), clinical attachment loss (CAL), and the dental caries index (DMFT)</span><span> recorded as w</span><span>ell as salivary pH. All findings collected, then analyzed by an</span><span> ANOVA test and the t</span><span>-test. A p-value at <0.05 was considered a statistical significance level.</span><span> </span><b><span>R</span></b><b><span>esults:</span></b><b><span> </span></b><span>The clinical findings of the current study showed that there were statistically significant differences in the patients’ age of both males and females. Moreover, there were statistically significant diffe</span><span>rences in GI. On the other hand, there were highly statistically significant differences in PLI and DMFS, but there were no significant differences</span><span> in CAL of both males and females </span><span>Moreover, there were no statistically significant differences in salivary pH</span><span>.</span><span> </span><b><span>Conclusion:</span></b><span> We concluded that DMFT Index correlated to periodontal and oral hea</span><span>lth status and, it increased with the progression of the pat</span><span>ient’s age and</span><span> can be used in the epidemiological evaluation of periodontal and o</span><span>ral health status.展开更多
To investigate the biological character of human adipose-derived adult stem cells (hADAS cells) when cultured in vitro and the relationship between hADAS cell’s replication activity and the donor’s age factor, and t...To investigate the biological character of human adipose-derived adult stem cells (hADAS cells) when cultured in vitro and the relationship between hADAS cell’s replication activity and the donor’s age factor, and to assess the stem cells as a new source for tissue engineering. hADAS cells are isolated from human adipose tissue of different age groups (from adolescents to olds: <20 years old, 21―40 years old, 41―60 years old and >61 years old groups). The protein markers (CD29, CD34, CD44, CD45, CD49d, HLA-DR, CD106) of hADAS cells were detected by flow cytometry (FCM) to identify the stem cell, and the cell cycle was examined for P20 hADAS cells to evaluate the safety of the subculture in vitro. The generative activity of hADAS cells in different age groups was also examined by MTT method. The formula “ log2T D = t logN t ? logN 0” was used to get the time doubling (TD) of the cells. The results showed that the cells kept heredity stabilization by chromosome analysis for at least 20 passages. The TD of these cells increased progressively by ageing, and the TD of the <20 years old group was lower than that of the >61 years old group (statistical analysis of variance (ANOVA), P=0.002, P<0.05). These find- ings suggested that a higher level of hADAS cells replication activity was found in the younger dona- tors, and they represent novel and valuable seed cells for studies of tissue engineering.展开更多
文摘<b><span>Background:</span></b><span> The patients’ age is considered a modified risk factor that causes periodontal disease, dental caries, and their development.</span><b><span> Objective:</span></b><span> The present study was designed to evaluate the impact of salivary pH value and dental caries index on periodontal status among adult age groups of patients from Aseer region, Saudi Arabia</span><span>.</span><span> </span><b><span>Methods:</span></b><span> A cross-sectional analysi</span><span>s carried out in the college of dentistry, King Khalid University on 750 </span><span>adult patients (400 males and 350 females). The adults patients were divided into three equal groups according to the patients’ age: group I (20 - 30 years), group I</span><span>I (31 - 40 years), and group III (41 - 50 years).</span><span> </span><span>Plaque index (PLI), gingi</span><span>val index (GI), clinical attachment loss (CAL), and the dental caries index (DMFT)</span><span> recorded as w</span><span>ell as salivary pH. All findings collected, then analyzed by an</span><span> ANOVA test and the t</span><span>-test. A p-value at <0.05 was considered a statistical significance level.</span><span> </span><b><span>R</span></b><b><span>esults:</span></b><b><span> </span></b><span>The clinical findings of the current study showed that there were statistically significant differences in the patients’ age of both males and females. Moreover, there were statistically significant diffe</span><span>rences in GI. On the other hand, there were highly statistically significant differences in PLI and DMFS, but there were no significant differences</span><span> in CAL of both males and females </span><span>Moreover, there were no statistically significant differences in salivary pH</span><span>.</span><span> </span><b><span>Conclusion:</span></b><span> We concluded that DMFT Index correlated to periodontal and oral hea</span><span>lth status and, it increased with the progression of the pat</span><span>ient’s age and</span><span> can be used in the epidemiological evaluation of periodontal and o</span><span>ral health status.
基金the Science and Technical Research Funds of Guangdong Province, China (Grant Nos. 2004B34001004 and04009423)
文摘To investigate the biological character of human adipose-derived adult stem cells (hADAS cells) when cultured in vitro and the relationship between hADAS cell’s replication activity and the donor’s age factor, and to assess the stem cells as a new source for tissue engineering. hADAS cells are isolated from human adipose tissue of different age groups (from adolescents to olds: <20 years old, 21―40 years old, 41―60 years old and >61 years old groups). The protein markers (CD29, CD34, CD44, CD45, CD49d, HLA-DR, CD106) of hADAS cells were detected by flow cytometry (FCM) to identify the stem cell, and the cell cycle was examined for P20 hADAS cells to evaluate the safety of the subculture in vitro. The generative activity of hADAS cells in different age groups was also examined by MTT method. The formula “ log2T D = t logN t ? logN 0” was used to get the time doubling (TD) of the cells. The results showed that the cells kept heredity stabilization by chromosome analysis for at least 20 passages. The TD of these cells increased progressively by ageing, and the TD of the <20 years old group was lower than that of the >61 years old group (statistical analysis of variance (ANOVA), P=0.002, P<0.05). These find- ings suggested that a higher level of hADAS cells replication activity was found in the younger dona- tors, and they represent novel and valuable seed cells for studies of tissue engineering.