Objective: Colchicine induced a non-protective Th2-like immunity in Aggregatibacter actinomycetemcomitans-stimulated murine immune response. The aim of the present study was to determine whether colchicine affects ind...Objective: Colchicine induced a non-protective Th2-like immunity in Aggregatibacter actinomycetemcomitans-stimulated murine immune response. The aim of the present study was to determine whether colchicine affects inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) production in A. actinomycetemcomitans-immunized mice. Materials and Methods: BALB/c mice were sham-immunized (group I) or immunized with heat-killed A. actinomycetemcomitans (group II-VII). Colchicine was injected intraperitoneally before (group III), on the same day of (group IV), or after (group V) the primary immunization and on the same day of (group VI) or after (group VII) the secondary immunization. In vitro, spleen cells from either sham- or heat-killed A. actinomycetemcomitan-immunized animals were cultured and stimulated with heat-killed A. actinomycetemcomitans in the presence or absence of colchicine with or without addition of L-arginine, Db-cAMP, forskolin or interferon-γ (IFN-γ). The levels of splenic iNOS activity and both serum and culture supernatant NO levels were assessed. Results: The results showed that colchicine did inhibit both splenic iNOS activity and serum NO levels only when the drug was injected at the same time as the immunization (group IV and VI). Splenic iNOS activity and NO levels on antigen-stimulated spleen cell cultures were also suppressed by colchicine, even in the presence of L-arginine, Db-AMP or forskolin. IFN-γ only partially restored iNOS activity and NO levels in the antigen and colchicine-treated spleen cell cultures. Conclusion: This study suggests, therefore, that colchicine may suppress the iNOS activity and NO production in A. actinomycetemcomitans-immunized mice in vivo and in vitro.展开更多
Objective: To determine Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) isolated from periodontal patients and healthy subjects using culture and PCR methods. Methods: Duplicate paper point needles we...Objective: To determine Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) isolated from periodontal patients and healthy subjects using culture and PCR methods. Methods: Duplicate paper point needles were taken from 100 samples (50 healthy subjects and 50 patients), who referred to the specialized dental clinic from Oct. 2015 to Mar. 2016. In laboratory after incubation period and observing the star-shaped colony A. actinomycetemcomitans, the confirmation tests, including gram staining and catalase test were carried out. For PCR, samples were analyzed with genus specific primers. These primers set, amplified a 500 bp fragment. Results: Of the 100 samples, A. actinomycetemcomitans was isolated from 31 patients (31%), (24 isolate of patients, and 7 isolate of healthy subjects) by using a selective Aggregatibacter isolation medium. Using PCR, a total of 49 (49%) samples were found to be positive for A. actinomycetemcomitans (35 isolate of patients, and 14 isolate of healthy subjects). Conclusion: PCR was found to be highly sensitive when genus specific primers were used for diagnosis of A. actinomycetemcomitans in comparison with culture method.展开更多
A number of studies have indicated that bacteria able to emit red fluorescence can be detected by light in-duced fluorescence technique and killed by photodynamic therapy. The objective of this study was to investigat...A number of studies have indicated that bacteria able to emit red fluorescence can be detected by light in-duced fluorescence technique and killed by photodynamic therapy. The objective of this study was to investigate the red fluorescence properties of the single gram negative capnophilic bacterium Aggregatibacter actinomycetemcomitans, ATCC 33384, and to investigate if these properties were related to the growth, morphology and size of the bacterial colonies. Time trend assessment was made with red fluorescence by QLF (Quantitative Light-induced Fluorescence), as well as with white light digital imaging. It was demonstrated that A. actinomycetemcomitans, a single capnophilic bacterium, is able to produce red fluorescence on its own, i.e. in the absence of other bacteria strains, and that blood agar is necessary to obtain red fluorescence from this bacterium on culture plates. This bacterium formed smooth circular, bell/dome like colonies increasing in size with time exhibiting various red fluorescence behaviors. A large variation in the fluorescence behavior points out an inhomogeneous distribution of red fluorescence within and between the colonies, i.e. the size of the investigated colonies did not correlate with the red fluorescence area, suggesting a dependence on the colony morphology such as the colony growth in height. To our knowledge this is the first study that have shown that A. actinomycetemcomitans on its own is able to produce fluorescence in the red spectral region.展开更多
目的探讨磷脂酰胆碱在伴放线放线杆菌(Aggregatibacter actinomycetemcomitans,Aa)对人脐静脉血管内皮细胞粘附和侵袭过程中的作用。方法采集7例侵袭性牙周炎患者牙周袋内的非附着性菌斑,接种于Aa选择性培养板上,根据菌落的形态、触酶...目的探讨磷脂酰胆碱在伴放线放线杆菌(Aggregatibacter actinomycetemcomitans,Aa)对人脐静脉血管内皮细胞粘附和侵袭过程中的作用。方法采集7例侵袭性牙周炎患者牙周袋内的非附着性菌斑,接种于Aa选择性培养板上,根据菌落的形态、触酶实验、革兰氏染色实验及16S r DNA鉴定及细菌表面蛋白鉴定,筛选出磷脂酰胆碱阳性表达的Aa菌株,并通过粘附侵袭实验,研究磷脂酰胆碱在Aa致病中的作用。结果侵袭性牙周炎患者的龈下菌斑通过Aa的选择性培养及鉴定,筛选出了1株磷脂酰胆碱阳性的Aa菌株,在粘附和侵袭实验中,发现磷脂酰胆碱被其特异性单克隆抗体小鼠抗磷脂酰胆碱单克隆抗体TEPC-15阻断后,磷脂酰胆碱阳性的Aa对人脐静脉血管内皮细胞的粘附率和侵袭率分别为对照组的(31.87±4.22)%和(24.63±3.55)%,粘附率(t=27.981,P=0.001)和侵袭率(t=36.786,P=0.001)与对照组相比差异均有统计学意义。结论磷脂酰胆碱有助于Aa对人脐静脉血管内皮细胞的粘附和入侵。展开更多
文摘Objective: Colchicine induced a non-protective Th2-like immunity in Aggregatibacter actinomycetemcomitans-stimulated murine immune response. The aim of the present study was to determine whether colchicine affects inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) production in A. actinomycetemcomitans-immunized mice. Materials and Methods: BALB/c mice were sham-immunized (group I) or immunized with heat-killed A. actinomycetemcomitans (group II-VII). Colchicine was injected intraperitoneally before (group III), on the same day of (group IV), or after (group V) the primary immunization and on the same day of (group VI) or after (group VII) the secondary immunization. In vitro, spleen cells from either sham- or heat-killed A. actinomycetemcomitan-immunized animals were cultured and stimulated with heat-killed A. actinomycetemcomitans in the presence or absence of colchicine with or without addition of L-arginine, Db-cAMP, forskolin or interferon-γ (IFN-γ). The levels of splenic iNOS activity and both serum and culture supernatant NO levels were assessed. Results: The results showed that colchicine did inhibit both splenic iNOS activity and serum NO levels only when the drug was injected at the same time as the immunization (group IV and VI). Splenic iNOS activity and NO levels on antigen-stimulated spleen cell cultures were also suppressed by colchicine, even in the presence of L-arginine, Db-AMP or forskolin. IFN-γ only partially restored iNOS activity and NO levels in the antigen and colchicine-treated spleen cell cultures. Conclusion: This study suggests, therefore, that colchicine may suppress the iNOS activity and NO production in A. actinomycetemcomitans-immunized mice in vivo and in vitro.
文摘Objective: To determine Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) isolated from periodontal patients and healthy subjects using culture and PCR methods. Methods: Duplicate paper point needles were taken from 100 samples (50 healthy subjects and 50 patients), who referred to the specialized dental clinic from Oct. 2015 to Mar. 2016. In laboratory after incubation period and observing the star-shaped colony A. actinomycetemcomitans, the confirmation tests, including gram staining and catalase test were carried out. For PCR, samples were analyzed with genus specific primers. These primers set, amplified a 500 bp fragment. Results: Of the 100 samples, A. actinomycetemcomitans was isolated from 31 patients (31%), (24 isolate of patients, and 7 isolate of healthy subjects) by using a selective Aggregatibacter isolation medium. Using PCR, a total of 49 (49%) samples were found to be positive for A. actinomycetemcomitans (35 isolate of patients, and 14 isolate of healthy subjects). Conclusion: PCR was found to be highly sensitive when genus specific primers were used for diagnosis of A. actinomycetemcomitans in comparison with culture method.
基金supported by a grant from StureNymans Commemoration Fund
文摘A number of studies have indicated that bacteria able to emit red fluorescence can be detected by light in-duced fluorescence technique and killed by photodynamic therapy. The objective of this study was to investigate the red fluorescence properties of the single gram negative capnophilic bacterium Aggregatibacter actinomycetemcomitans, ATCC 33384, and to investigate if these properties were related to the growth, morphology and size of the bacterial colonies. Time trend assessment was made with red fluorescence by QLF (Quantitative Light-induced Fluorescence), as well as with white light digital imaging. It was demonstrated that A. actinomycetemcomitans, a single capnophilic bacterium, is able to produce red fluorescence on its own, i.e. in the absence of other bacteria strains, and that blood agar is necessary to obtain red fluorescence from this bacterium on culture plates. This bacterium formed smooth circular, bell/dome like colonies increasing in size with time exhibiting various red fluorescence behaviors. A large variation in the fluorescence behavior points out an inhomogeneous distribution of red fluorescence within and between the colonies, i.e. the size of the investigated colonies did not correlate with the red fluorescence area, suggesting a dependence on the colony morphology such as the colony growth in height. To our knowledge this is the first study that have shown that A. actinomycetemcomitans on its own is able to produce fluorescence in the red spectral region.
文摘目的探讨磷脂酰胆碱在伴放线放线杆菌(Aggregatibacter actinomycetemcomitans,Aa)对人脐静脉血管内皮细胞粘附和侵袭过程中的作用。方法采集7例侵袭性牙周炎患者牙周袋内的非附着性菌斑,接种于Aa选择性培养板上,根据菌落的形态、触酶实验、革兰氏染色实验及16S r DNA鉴定及细菌表面蛋白鉴定,筛选出磷脂酰胆碱阳性表达的Aa菌株,并通过粘附侵袭实验,研究磷脂酰胆碱在Aa致病中的作用。结果侵袭性牙周炎患者的龈下菌斑通过Aa的选择性培养及鉴定,筛选出了1株磷脂酰胆碱阳性的Aa菌株,在粘附和侵袭实验中,发现磷脂酰胆碱被其特异性单克隆抗体小鼠抗磷脂酰胆碱单克隆抗体TEPC-15阻断后,磷脂酰胆碱阳性的Aa对人脐静脉血管内皮细胞的粘附率和侵袭率分别为对照组的(31.87±4.22)%和(24.63±3.55)%,粘附率(t=27.981,P=0.001)和侵袭率(t=36.786,P=0.001)与对照组相比差异均有统计学意义。结论磷脂酰胆碱有助于Aa对人脐静脉血管内皮细胞的粘附和入侵。
