Ribosonal protein L21 which is a component of the 60s large ribosomal subunit plays an important role in ribosome. To explore the structure characteristic of ribosomal protein L21(rpL21) gene of the Ailuropoda melanol...Ribosonal protein L21 which is a component of the 60s large ribosomal subunit plays an important role in ribosome. To explore the structure characteristic of ribosomal protein L21(rpL21) gene of the Ailuropoda melanoleuca and investigate its homologies with other already reported sequenses' including Rattus norvegicus, Mus musculus, Mus musculus, etc. The cDNA of rpL21 was cloned from the Ailuropoda melanoleuca by RT-PCR. The sequence data were analyzed by Genscan software. Blast 2.1 was used to study the homology of the obtained rpL21 sequence with the gene sequence of other species; Open reading frame (ORF) of the DNA sequence was searched using ORF finder software; Protein structure of the rpL21 sequence cloned was deduced using Predict Protein software. The results indicated that the length of cDNA fragment cloned was 504bp; containing an open reading frame of 483bp. Deduced protein was composed of 160 amino acids with an estimated molecular weight of 18.59kD and pI of 11.10. The length of the genomic sequence is 2254bp, containing 5 exons and 4 introns. Alignment analysis indicates that rpL21 is highly similarity with the reported species both at the level of DNA and protein. Topology prediction shows that 6 different patterns were found in the rpL21 protein of the Ailuropoda melanoleuca. The rpL21 gene of the Ailuropoda melanoleuca was studied in this paper, which will enrich and improve the mammals' rpL21 gene database.展开更多
晚更新世的大熊猫广泛分布于中国华南地区,化石分布点众多,但完整保存的骨骼材料相对较少。文中报道了贵州惠水县仰天窝竖井新发现的完整大熊猫头骨化石,仰天窝竖井—白马洞因其特殊的洞穴环境为化石的保藏创造了有利条件。根据测量数据...晚更新世的大熊猫广泛分布于中国华南地区,化石分布点众多,但完整保存的骨骼材料相对较少。文中报道了贵州惠水县仰天窝竖井新发现的完整大熊猫头骨化石,仰天窝竖井—白马洞因其特殊的洞穴环境为化石的保藏创造了有利条件。根据测量数据,仰天窝大熊猫个体牙齿比现生大熊猫略大,应属于巴氏大熊猫。AMS—^(14)C测年结果为22887~22431 a BP,时代处于末次盛冰期前后,新材料的发现为推断大熊猫在晚更新世末期的分布范围、生存演化等问题提供了难得的实证。展开更多
大熊猫晚更新世广泛分布于我国华南地区,化石点众多,但完整头骨材料相对较少。本文报道了贵州惠水仰天窝竖井最新发现的大熊猫头骨及下颌骨化石,保存相对较完整。仰天窝竖井—白马洞特殊的洞穴条件为化石的保存提供了有利条件。根据测...大熊猫晚更新世广泛分布于我国华南地区,化石点众多,但完整头骨材料相对较少。本文报道了贵州惠水仰天窝竖井最新发现的大熊猫头骨及下颌骨化石,保存相对较完整。仰天窝竖井—白马洞特殊的洞穴条件为化石的保存提供了有利条件。根据测量数据,仰天窝大熊猫个体牙齿比现生大熊猫略大,应属于巴氏大熊猫。AMS-^(14)C测年结果为22887~22431 a BP,处于末次盛冰期前后.展开更多
基金financially supported by Application Foundation Project in Sichuan Province(2009JY0061)Youth Fund Project of Educational Committee of Sichuan province(09ZB088)
文摘Ribosonal protein L21 which is a component of the 60s large ribosomal subunit plays an important role in ribosome. To explore the structure characteristic of ribosomal protein L21(rpL21) gene of the Ailuropoda melanoleuca and investigate its homologies with other already reported sequenses' including Rattus norvegicus, Mus musculus, Mus musculus, etc. The cDNA of rpL21 was cloned from the Ailuropoda melanoleuca by RT-PCR. The sequence data were analyzed by Genscan software. Blast 2.1 was used to study the homology of the obtained rpL21 sequence with the gene sequence of other species; Open reading frame (ORF) of the DNA sequence was searched using ORF finder software; Protein structure of the rpL21 sequence cloned was deduced using Predict Protein software. The results indicated that the length of cDNA fragment cloned was 504bp; containing an open reading frame of 483bp. Deduced protein was composed of 160 amino acids with an estimated molecular weight of 18.59kD and pI of 11.10. The length of the genomic sequence is 2254bp, containing 5 exons and 4 introns. Alignment analysis indicates that rpL21 is highly similarity with the reported species both at the level of DNA and protein. Topology prediction shows that 6 different patterns were found in the rpL21 protein of the Ailuropoda melanoleuca. The rpL21 gene of the Ailuropoda melanoleuca was studied in this paper, which will enrich and improve the mammals' rpL21 gene database.
文摘晚更新世的大熊猫广泛分布于中国华南地区,化石分布点众多,但完整保存的骨骼材料相对较少。文中报道了贵州惠水县仰天窝竖井新发现的完整大熊猫头骨化石,仰天窝竖井—白马洞因其特殊的洞穴环境为化石的保藏创造了有利条件。根据测量数据,仰天窝大熊猫个体牙齿比现生大熊猫略大,应属于巴氏大熊猫。AMS—^(14)C测年结果为22887~22431 a BP,时代处于末次盛冰期前后,新材料的发现为推断大熊猫在晚更新世末期的分布范围、生存演化等问题提供了难得的实证。
文摘大熊猫晚更新世广泛分布于我国华南地区,化石点众多,但完整头骨材料相对较少。本文报道了贵州惠水仰天窝竖井最新发现的大熊猫头骨及下颌骨化石,保存相对较完整。仰天窝竖井—白马洞特殊的洞穴条件为化石的保存提供了有利条件。根据测量数据,仰天窝大熊猫个体牙齿比现生大熊猫略大,应属于巴氏大熊猫。AMS-^(14)C测年结果为22887~22431 a BP,处于末次盛冰期前后.
