目的探讨骨髓间充质干细胞外泌体(exosomes derived from bone marrow mesenchymal stem cell,BMSCs-Exos)通过PI3K/Akt通路修复皮瓣缺血再灌注损伤的机制。方法培养大鼠骨髓间充质干细胞(BMSCs),提取细胞上清液中的外泌体并通过电镜、...目的探讨骨髓间充质干细胞外泌体(exosomes derived from bone marrow mesenchymal stem cell,BMSCs-Exos)通过PI3K/Akt通路修复皮瓣缺血再灌注损伤的机制。方法培养大鼠骨髓间充质干细胞(BMSCs),提取细胞上清液中的外泌体并通过电镜、粒径分析及免疫印迹实验进行鉴定。建立以腹壁浅动静脉为血管蒂的大鼠腹部皮瓣缺血再灌注损伤模型;将大鼠随机分为4组:Sham组、IR+PBS组、IR+Exo组及IR+Exo+LY组。术后观察并拍照记录大鼠皮瓣大体情况,第7天计算皮瓣坏死区比例;获取大鼠皮瓣组织样本,HE染色和组织评分评估皮瓣组织损伤程度,免疫组化评估皮瓣p-Akt、VEGFA表达水平和CD34标记的微血管密度。结果相比Sham组,IR+PBS组皮瓣坏死区比例增加,组织结构破坏加重,p-Akt及VEGFA表达水平下降,微血管密度降低;IR+Exo组皮瓣坏死和组织破坏水平降低,p-Akt及VEGFA表达上升,微血管密度增加;而PI3K抑制剂降低了BMSCs外泌体的修复作用,表现为皮瓣坏死增加,p-Akt及VEGFA表达下调,微血管密度减少。结论BMSCs外泌体对大鼠腹部皮瓣缺血再灌注损伤具有显著的保护和修复作用,PI3K/Akt信号通路可能是BMSCs外泌体治疗皮瓣缺血再灌注损伤的关键通路。展开更多
由于其强度的侵犯和高程度的不稳定特性,胃癌被认为是种复杂且难以处理的情况;它通常会激活多个信号途径以影响许多生物过程。PI3K-Akt蛋白质活性调节器是一个关键的路径系统,对各种病状有着重要关联并且近年来越来受到研究者的关注:因...由于其强度的侵犯和高程度的不稳定特性,胃癌被认为是种复杂且难以处理的情况;它通常会激活多个信号途径以影响许多生物过程。PI3K-Akt蛋白质活性调节器是一个关键的路径系统,对各种病状有着重要关联并且近年来越来受到研究者的关注:因为它们能够刺激大量基因产物的产生而这些物质又可引发一系列生理效应。另一方面,中国传统医学拥有深厚的根基并在长期实践中积累了丰富的经验及独特的能力去协调身体各部分的功能从而预防或减缓疾病的进展——这其中包括对于消化道恶性病变如胃癌的影响上也表现出显著的效果。中医疗法不仅能在一定程度上缓解手术或者化学药物带来的不良后果还能减少一些可能出现的合并症状甚至有抑制瘤体扩散的可能进而提升患者的治愈几率同时也能改善他们的生活品质延长大致的生活年限。因此,我们将对PI3K/Akt信号通路进行全面的系统性分析,以了解近些年来学者们如何运用中医药治疗胃癌。这包括了胃癌细胞的增殖、凋亡、侵袭转移、癌前病变和生长周期等方面。通过研究自噬、耐药性等多个生物学途径,可以明确中医药是如何通过PI3K/Akt信号通路来干预胃癌的过程,从而为胃癌治疗提供新的思路。Because of its intense invasion and high degree of instability, gastric cancer is considered to be complex and difficult to deal with;it usually activates multiple signal pathways to affect many biological processes. PI3K-Akt protein activity regulator is a key pathway system, which has an important relationship with a variety of diseases and has attracted more and more attention of researchers in recent years, because they can stimulate the production of a large number of gene products, and these substances can cause a series of physiological effects. On the other hand, Chinese traditional medicine has a deep foundation and has accumulated rich experience and unique ability in long-term practice to coordinate the functions of various parts of the body so as to prevent or slow down the progress of the disease, including malignant lesions of the digestive tract such as gastric cancer. Traditional Chinese medicine therapy can not only alleviate the adverse consequences caused by surgery or chemical drugs to a certain extent, but also reduce some possible concomitant symptoms and even inhibit the spread of tumors, so as to improve the cure probability of patients, but also improve their quality of life and prolong their general life years. Therefore, we will conduct a comprehensive and systematic analysis of the PI3K/Akt signal pathway in order to understand how scholars use traditional Chinese medicine to treat gastric cancer in recent years. This includes the proliferation, apoptosis, invasion and metastasis, precancerous lesions and growth cycle of gastric cancer cells. Through the study of autophagy, drug resistance and other biological pathways, we can clarify how traditional Chinese medicine interferes with the process of gastric cancer through PI3K/Akt signal pathway, so as to provide new ideas for the treatment of gastric cancer.展开更多
文摘目的探讨骨髓间充质干细胞外泌体(exosomes derived from bone marrow mesenchymal stem cell,BMSCs-Exos)通过PI3K/Akt通路修复皮瓣缺血再灌注损伤的机制。方法培养大鼠骨髓间充质干细胞(BMSCs),提取细胞上清液中的外泌体并通过电镜、粒径分析及免疫印迹实验进行鉴定。建立以腹壁浅动静脉为血管蒂的大鼠腹部皮瓣缺血再灌注损伤模型;将大鼠随机分为4组:Sham组、IR+PBS组、IR+Exo组及IR+Exo+LY组。术后观察并拍照记录大鼠皮瓣大体情况,第7天计算皮瓣坏死区比例;获取大鼠皮瓣组织样本,HE染色和组织评分评估皮瓣组织损伤程度,免疫组化评估皮瓣p-Akt、VEGFA表达水平和CD34标记的微血管密度。结果相比Sham组,IR+PBS组皮瓣坏死区比例增加,组织结构破坏加重,p-Akt及VEGFA表达水平下降,微血管密度降低;IR+Exo组皮瓣坏死和组织破坏水平降低,p-Akt及VEGFA表达上升,微血管密度增加;而PI3K抑制剂降低了BMSCs外泌体的修复作用,表现为皮瓣坏死增加,p-Akt及VEGFA表达下调,微血管密度减少。结论BMSCs外泌体对大鼠腹部皮瓣缺血再灌注损伤具有显著的保护和修复作用,PI3K/Akt信号通路可能是BMSCs外泌体治疗皮瓣缺血再灌注损伤的关键通路。
文摘由于其强度的侵犯和高程度的不稳定特性,胃癌被认为是种复杂且难以处理的情况;它通常会激活多个信号途径以影响许多生物过程。PI3K-Akt蛋白质活性调节器是一个关键的路径系统,对各种病状有着重要关联并且近年来越来受到研究者的关注:因为它们能够刺激大量基因产物的产生而这些物质又可引发一系列生理效应。另一方面,中国传统医学拥有深厚的根基并在长期实践中积累了丰富的经验及独特的能力去协调身体各部分的功能从而预防或减缓疾病的进展——这其中包括对于消化道恶性病变如胃癌的影响上也表现出显著的效果。中医疗法不仅能在一定程度上缓解手术或者化学药物带来的不良后果还能减少一些可能出现的合并症状甚至有抑制瘤体扩散的可能进而提升患者的治愈几率同时也能改善他们的生活品质延长大致的生活年限。因此,我们将对PI3K/Akt信号通路进行全面的系统性分析,以了解近些年来学者们如何运用中医药治疗胃癌。这包括了胃癌细胞的增殖、凋亡、侵袭转移、癌前病变和生长周期等方面。通过研究自噬、耐药性等多个生物学途径,可以明确中医药是如何通过PI3K/Akt信号通路来干预胃癌的过程,从而为胃癌治疗提供新的思路。Because of its intense invasion and high degree of instability, gastric cancer is considered to be complex and difficult to deal with;it usually activates multiple signal pathways to affect many biological processes. PI3K-Akt protein activity regulator is a key pathway system, which has an important relationship with a variety of diseases and has attracted more and more attention of researchers in recent years, because they can stimulate the production of a large number of gene products, and these substances can cause a series of physiological effects. On the other hand, Chinese traditional medicine has a deep foundation and has accumulated rich experience and unique ability in long-term practice to coordinate the functions of various parts of the body so as to prevent or slow down the progress of the disease, including malignant lesions of the digestive tract such as gastric cancer. Traditional Chinese medicine therapy can not only alleviate the adverse consequences caused by surgery or chemical drugs to a certain extent, but also reduce some possible concomitant symptoms and even inhibit the spread of tumors, so as to improve the cure probability of patients, but also improve their quality of life and prolong their general life years. Therefore, we will conduct a comprehensive and systematic analysis of the PI3K/Akt signal pathway in order to understand how scholars use traditional Chinese medicine to treat gastric cancer in recent years. This includes the proliferation, apoptosis, invasion and metastasis, precancerous lesions and growth cycle of gastric cancer cells. Through the study of autophagy, drug resistance and other biological pathways, we can clarify how traditional Chinese medicine interferes with the process of gastric cancer through PI3K/Akt signal pathway, so as to provide new ideas for the treatment of gastric cancer.
