[Objective] The research aimed to select the buckwheat accessions with higher Al3＋ tolerance.[Method]25 buckwheat accessions were used as materials,the Al3＋ tolerance were studied by means of solution culture method...[Objective] The research aimed to select the buckwheat accessions with higher Al3＋ tolerance.[Method]25 buckwheat accessions were used as materials,the Al3＋ tolerance were studied by means of solution culture method in this research.The length of root was used to evaluate the degree of Al3＋ tolerance.[Result]The root growth of some buckwheat accessions were significantly promoted under the concentration of 500 μmol/L,while high concentration of Al3＋（1 000 μmol/L）inhibited the buckwheat roots growth.There were great variations of Al3＋ tolerance among the different buckwheat genotypes.[Conclusion]Among the 25 buckwheat genotypes,L2081 and T442 had much higher Al3＋ tolerance than the others,which could be used for research on the buckwheat breeding and the mechanism of the buckwheat Al3＋ tolerance.展开更多
印染废水中过高浓度的Al^(3+)不仅会破坏生态环境,而且还将危害人类健康。因此,对印染废水中Al^(3+)浓度的精准检测具有十分重要的研究意义。本文以罗丹明B、乙二胺和4-甲酰基-3-羟基苯甲酸为原料,合成了新型荧光探针RhB-AC,并利用高分...印染废水中过高浓度的Al^(3+)不仅会破坏生态环境,而且还将危害人类健康。因此,对印染废水中Al^(3+)浓度的精准检测具有十分重要的研究意义。本文以罗丹明B、乙二胺和4-甲酰基-3-羟基苯甲酸为原料,合成了新型荧光探针RhB-AC,并利用高分辨质谱(HRMS)及核磁共振氢谱(1H NMR)对探针RhB-AC的分子结构进行了表征。探针RhB-AC在水中,由于光诱导的电子转移(Photoinduced electron transfer,PET)效应,其荧光发生猝灭。当与Al^(3+)作用后,PET效应被抑制,探针表现出很强的青绿色荧光。另外,该探针对Al^(3+)检测的速度快(3秒以内)、选择性好、灵敏度高(检测限为16 nM)。最后,该探针还能快速、定量检测实际印染废水中的Al^(3+)浓度。展开更多
Central tolerance in the thymus is the primary mechanism for deleting autoreactive T cells. Despite this, escape of self-reactive T lymphocytes into the periphery reveals the threat of autoimmunity. To compensate for ...Central tolerance in the thymus is the primary mechanism for deleting autoreactive T cells. Despite this, escape of self-reactive T lymphocytes into the periphery reveals the threat of autoimmunity. To compensate for its imperfection, the thymus also produces a naturally occurring subset of Foxp3＋ CD4＋CD25＋ regulatory T cells with suppressive function, capable of controlling autoreactive cells. Foxp3 （forkhead box P3）, the lineage-specific marker for this subset of cells, is crucial to their thymic development and peripheral function, and yet the transcriptional program driven by Foxp3 was until now largely undefined. Emerging evidence has provided insight into its role： from the ability of Foxp3 to cooperate with other transcription factors such as NFAT, to the genome-wide characterization of target genes directly bound and regulated by Foxp3. Here we discuss the discovery of naturally occurring regulatory T cells-their phenotype, development, maintenance, and function-largely as they are defined by the lineage-specific marker, Foxp3.展开更多
The involvement of apoptosis in mitochondrial toxin 3 nitropropionic acid (3 NPA) induced ischemic tolerance to transient focal cerebral ischemia in rats and the mechanism was investigated. 3 NPA at a dose of 20 m...The involvement of apoptosis in mitochondrial toxin 3 nitropropionic acid (3 NPA) induced ischemic tolerance to transient focal cerebral ischemia in rats and the mechanism was investigated. 3 NPA at a dose of 20 mg/kg or vehicle control was intraperitoneally into the rats. Three days later, rats were exposed to 2 h of middle cerebral artery occlusion followed by 24 h of reperfusion. Infarct volumes were assessed by 2,3,5 triphenyltetrazolinm chloride (TTC) staining 24 h after reperfusion. Neural cell apoptosis in cerebral ischemic penumbra was detected by terminal deoxynucleotidyl transferase mediated dUTP biotin in situ nick end labeling (TUNEL) and flow cytometry methods (FCM). The results showed that as compared to the vehicle treated group, pretreatment with 3 NPA could reduce the infarct volume by 23.3 % and decrease the number of TUNEL positive neural cells and apoptotic percentage by 47 % ( P< 0.05) and 44.9 % ( P< 0 01), respectively. It was concluded that the development of 3 NPA induced ischemic tolerance in brain might be related to the decreases in neural cell apoptosis.展开更多
Heat stress is one of the main factors that inlfuence poultry production. Heat shock proteins (HSPs) are known to affect heat tolerance. The formation of HSPs is regulated by heat shock transcription factor 3 (HSF3) i...Heat stress is one of the main factors that inlfuence poultry production. Heat shock proteins (HSPs) are known to affect heat tolerance. The formation of HSPs is regulated by heat shock transcription factor 3 (HSF3) in chicken. A DNA pool was established for identifying single nucleotide polymorphisms (SNPs) of the chicken HSF3, and 13 SNPs were detected. The bioinformatic analysis showed that 8 SNPs had the capacity to alter the transcription activity of HSF3. The dual luciferase report gene assay showed that there was a signiifcant difference (P<0.01) in the Firelfy luciferase/Renil a luciferase ratio (F/R) of C.–1 703 A>G (S1) and C.–1 388 A>G (S4) sites at the 5′-untranslated region (UTR) of chicken HSF3. The elec-trophoretic mobility shift assay showed that the S4 site was a transcription binding factor. The analysis of the association of the S1 and S4 sites with heat tolerance index revealed that the S4 site was signiifcantly correlated with the CD3+T cel , corticosterone, and T3 levels in Lingshan chickens and with the heterophil/lymphocyte value in White Recessive Rock. These results showed that the S4 site at the 5′ UTR of chicken HSF3 might have an impact on heat tolerance in summer and could be used as a potential marker for the selection of chicken with heat tolerance in the future.展开更多
基金Supported by Science and Technology Foundation of Guizhou Province(QKH J[2009]2108)Doctoral Research Fund in Guizhou Normal University(2008)~~
文摘[Objective] The research aimed to select the buckwheat accessions with higher Al3＋ tolerance.[Method]25 buckwheat accessions were used as materials,the Al3＋ tolerance were studied by means of solution culture method in this research.The length of root was used to evaluate the degree of Al3＋ tolerance.[Result]The root growth of some buckwheat accessions were significantly promoted under the concentration of 500 μmol/L,while high concentration of Al3＋（1 000 μmol/L）inhibited the buckwheat roots growth.There were great variations of Al3＋ tolerance among the different buckwheat genotypes.[Conclusion]Among the 25 buckwheat genotypes,L2081 and T442 had much higher Al3＋ tolerance than the others,which could be used for research on the buckwheat breeding and the mechanism of the buckwheat Al3＋ tolerance.
文摘印染废水中过高浓度的Al^(3+)不仅会破坏生态环境,而且还将危害人类健康。因此,对印染废水中Al^(3+)浓度的精准检测具有十分重要的研究意义。本文以罗丹明B、乙二胺和4-甲酰基-3-羟基苯甲酸为原料,合成了新型荧光探针RhB-AC,并利用高分辨质谱(HRMS)及核磁共振氢谱(1H NMR)对探针RhB-AC的分子结构进行了表征。探针RhB-AC在水中,由于光诱导的电子转移(Photoinduced electron transfer,PET)效应,其荧光发生猝灭。当与Al^(3+)作用后,PET效应被抑制,探针表现出很强的青绿色荧光。另外,该探针对Al^(3+)检测的速度快(3秒以内)、选择性好、灵敏度高(检测限为16 nM)。最后,该探针还能快速、定量检测实际印染废水中的Al^(3+)浓度。
文摘Central tolerance in the thymus is the primary mechanism for deleting autoreactive T cells. Despite this, escape of self-reactive T lymphocytes into the periphery reveals the threat of autoimmunity. To compensate for its imperfection, the thymus also produces a naturally occurring subset of Foxp3＋ CD4＋CD25＋ regulatory T cells with suppressive function, capable of controlling autoreactive cells. Foxp3 （forkhead box P3）, the lineage-specific marker for this subset of cells, is crucial to their thymic development and peripheral function, and yet the transcriptional program driven by Foxp3 was until now largely undefined. Emerging evidence has provided insight into its role： from the ability of Foxp3 to cooperate with other transcription factors such as NFAT, to the genome-wide characterization of target genes directly bound and regulated by Foxp3. Here we discuss the discovery of naturally occurring regulatory T cells-their phenotype, development, maintenance, and function-largely as they are defined by the lineage-specific marker, Foxp3.
基金ThisprojectwassupportedbyagrantfrominitiativeFoundationofNatioualEducationMinistryforscholarscomingbackfromothercountries (No .2 0 0 1 345)
文摘The involvement of apoptosis in mitochondrial toxin 3 nitropropionic acid (3 NPA) induced ischemic tolerance to transient focal cerebral ischemia in rats and the mechanism was investigated. 3 NPA at a dose of 20 mg/kg or vehicle control was intraperitoneally into the rats. Three days later, rats were exposed to 2 h of middle cerebral artery occlusion followed by 24 h of reperfusion. Infarct volumes were assessed by 2,3,5 triphenyltetrazolinm chloride (TTC) staining 24 h after reperfusion. Neural cell apoptosis in cerebral ischemic penumbra was detected by terminal deoxynucleotidyl transferase mediated dUTP biotin in situ nick end labeling (TUNEL) and flow cytometry methods (FCM). The results showed that as compared to the vehicle treated group, pretreatment with 3 NPA could reduce the infarct volume by 23.3 % and decrease the number of TUNEL positive neural cells and apoptotic percentage by 47 % ( P< 0.05) and 44.9 % ( P< 0 01), respectively. It was concluded that the development of 3 NPA induced ischemic tolerance in brain might be related to the decreases in neural cell apoptosis.
基金supported the National Key Technology R&D Program of China (2014BAD08B08)the Key Technology Research and Development Program of Guangdong Emerging Strategic Industries, China (2012A020800005)
文摘Heat stress is one of the main factors that inlfuence poultry production. Heat shock proteins (HSPs) are known to affect heat tolerance. The formation of HSPs is regulated by heat shock transcription factor 3 (HSF3) in chicken. A DNA pool was established for identifying single nucleotide polymorphisms (SNPs) of the chicken HSF3, and 13 SNPs were detected. The bioinformatic analysis showed that 8 SNPs had the capacity to alter the transcription activity of HSF3. The dual luciferase report gene assay showed that there was a signiifcant difference (P<0.01) in the Firelfy luciferase/Renil a luciferase ratio (F/R) of C.–1 703 A>G (S1) and C.–1 388 A>G (S4) sites at the 5′-untranslated region (UTR) of chicken HSF3. The elec-trophoretic mobility shift assay showed that the S4 site was a transcription binding factor. The analysis of the association of the S1 and S4 sites with heat tolerance index revealed that the S4 site was signiifcantly correlated with the CD3+T cel , corticosterone, and T3 levels in Lingshan chickens and with the heterophil/lymphocyte value in White Recessive Rock. These results showed that the S4 site at the 5′ UTR of chicken HSF3 might have an impact on heat tolerance in summer and could be used as a potential marker for the selection of chicken with heat tolerance in the future.
