W25 is a low-temperature-sensitive albino mu-tant line. Temperature not only controls thealbino phenotype expression of W25, but alsodetermines whether it could survive. When thetemperature is lower than 25℃, the lea...W25 is a low-temperature-sensitive albino mu-tant line. Temperature not only controls thealbino phenotype expression of W25, but alsodetermines whether it could survive. When thetemperature is lower than 25℃, the leaves ofW25 shows complete albino, but they exhibitsnormal green when temperature is higher than30℃. Meanwhile, at 25℃, it can be greenable展开更多
Albino mutants are useful genetic resource for studying chlorophyll biosynthesis and chloroplast development and cloning genes involved in these processes in plants. Here we report a novel rice mutant low temperature ...Albino mutants are useful genetic resource for studying chlorophyll biosynthesis and chloroplast development and cloning genes involved in these processes in plants. Here we report a novel rice mutant low temperature albino I （ltal） that showed albino leaves before 4-leaf stage when grown under temperature lower than 20℃, but developed normal green leaves under temperature higher than 24℃ or similar morphological phenotypes in dark as did the wild-type （WT）. Our analysis showed that the contents of chlorophylls and chlo- rophyll precursors were remarkably decreased in the Ital mutant under low temperature compared to WT. Transmission electron microscope observation revealed that chloroplasts were defectively developed in the albino ltal leaves, which lacked of well-stacked granum and contained less stroma lamellae. These results suggested that the ltal mutation may delay the light-induced thylakoid assembly under low temperature. Genetic analysis indicated that the albino phenotype was controlled by a single recessive locus. Through map-based approach, we finally located the Ltal gene to a region of 40.3 kb on the short arm of chromosome 11. There are 8 predicted open reading frames （ORFs） in this region and two of them were deleted in ltal genome compared with the WT genome. The further characterization of the Ltal gene would provide a good approach to uncover the novel molecular mechanisms involved in chloroplast development under low temperature stress.展开更多
W25 was a gamma-irradiation induced albinorice mutant line, which only expressed in thespecial temperatures (see figure). At 30 Cand 35 C, the seedling leaves of W25 showedgreenish or normal green, but they exhibiteda...W25 was a gamma-irradiation induced albinorice mutant line, which only expressed in thespecial temperatures (see figure). At 30 Cand 35 C, the seedling leaves of W25 showedgreenish or normal green, but they exhibitedalbino at 25℃, which could be greenish afterthe fourth leaf extension and recovered to be展开更多
为探讨水稻叶绿体发育的分子机制,通过对粳稻日本晴进行甲基磺酸乙酯(EMS)诱变,获得一个稳定遗传的叶色白化突变体wrg20(white turn green 20),并对其进行表型鉴定、基因定位和功能分析。与野生型(WT)相比,该突变体于30℃培养时在三叶...为探讨水稻叶绿体发育的分子机制,通过对粳稻日本晴进行甲基磺酸乙酯(EMS)诱变,获得一个稳定遗传的叶色白化突变体wrg20(white turn green 20),并对其进行表型鉴定、基因定位和功能分析。与野生型(WT)相比,该突变体于30℃培养时在三叶期之前完全白化,26℃时突变体白化叶片部分返绿。遗传分析结果表明,该突变性状受单隐性核基因控制。将该突变体与籼稻93-11杂交,构建F2分离群体并进行基因定位,将该基因定位于2号染色体198 kb区间内,通过测序发现LOC_Os02g33610存在由G至A单碱基替换,导致编码的天冬氨酸转化为天冬酰胺,表明该基因可能为OsWRG20的候选基因,与先前所报道的调控叶绿体发育的基因GRY79为等位基因。对该基因进行结构和功能分析,表明OsWRG20可能是调控水稻苗期幼叶生长发育的重要基因。与野生型相比,突变体的叶绿体基因内含子剪接效率降低,由此推断OsWRG20可能通过调控叶绿体RNA的剪接,参与调控水稻苗期叶绿体的发育。本研究结果为苗期水稻叶绿体发育研究提供了新的理论基础。展开更多
在水稻品种宜香B中发现了一个白化转绿叶突变体,经过多代自交获得了稳定的白化转绿叶色突变体。该突变体在4叶期前叶色为黄绿色,之后逐渐变绿,从苗龄4周到12周,突变体/野生型叶绿素含量比值从34.5%逐渐升高到99.4%。遗传分析表明该突变...在水稻品种宜香B中发现了一个白化转绿叶突变体,经过多代自交获得了稳定的白化转绿叶色突变体。该突变体在4叶期前叶色为黄绿色,之后逐渐变绿,从苗龄4周到12周,突变体/野生型叶绿素含量比值从34.5%逐渐升高到99.4%。遗传分析表明该突变受1对隐性核基因控制,暂命名为gra。利用微卫星标记将gra初步定位于第10染色体标记RM596和RM5620之间,进一步利用极端个体定位法把gra精细定位于标记RM25522和RM25535之间。gra基因距RM25522和RM25535标记的遗传距离均为0.05 c M,其物理距离约为136 kb。展开更多
文摘W25 is a low-temperature-sensitive albino mu-tant line. Temperature not only controls thealbino phenotype expression of W25, but alsodetermines whether it could survive. When thetemperature is lower than 25℃, the leaves ofW25 shows complete albino, but they exhibitsnormal green when temperature is higher than30℃. Meanwhile, at 25℃, it can be greenable
基金supported by the grants from the National Basic Research Program of China(No.2009CB119000)the Ministry of Agriculture of China for Transgenic Research (Nos.2011ZX08009-003,2011ZX08001-005)the National Science Foundation of China(Nos.31000094,30970246, 31100188 and 31161130533)
文摘Albino mutants are useful genetic resource for studying chlorophyll biosynthesis and chloroplast development and cloning genes involved in these processes in plants. Here we report a novel rice mutant low temperature albino I （ltal） that showed albino leaves before 4-leaf stage when grown under temperature lower than 20℃, but developed normal green leaves under temperature higher than 24℃ or similar morphological phenotypes in dark as did the wild-type （WT）. Our analysis showed that the contents of chlorophylls and chlo- rophyll precursors were remarkably decreased in the Ital mutant under low temperature compared to WT. Transmission electron microscope observation revealed that chloroplasts were defectively developed in the albino ltal leaves, which lacked of well-stacked granum and contained less stroma lamellae. These results suggested that the ltal mutation may delay the light-induced thylakoid assembly under low temperature. Genetic analysis indicated that the albino phenotype was controlled by a single recessive locus. Through map-based approach, we finally located the Ltal gene to a region of 40.3 kb on the short arm of chromosome 11. There are 8 predicted open reading frames （ORFs） in this region and two of them were deleted in ltal genome compared with the WT genome. The further characterization of the Ltal gene would provide a good approach to uncover the novel molecular mechanisms involved in chloroplast development under low temperature stress.
文摘W25 was a gamma-irradiation induced albinorice mutant line, which only expressed in thespecial temperatures (see figure). At 30 Cand 35 C, the seedling leaves of W25 showedgreenish or normal green, but they exhibitedalbino at 25℃, which could be greenish afterthe fourth leaf extension and recovered to be
文摘为探讨水稻叶绿体发育的分子机制,通过对粳稻日本晴进行甲基磺酸乙酯(EMS)诱变,获得一个稳定遗传的叶色白化突变体wrg20(white turn green 20),并对其进行表型鉴定、基因定位和功能分析。与野生型(WT)相比,该突变体于30℃培养时在三叶期之前完全白化,26℃时突变体白化叶片部分返绿。遗传分析结果表明,该突变性状受单隐性核基因控制。将该突变体与籼稻93-11杂交,构建F2分离群体并进行基因定位,将该基因定位于2号染色体198 kb区间内,通过测序发现LOC_Os02g33610存在由G至A单碱基替换,导致编码的天冬氨酸转化为天冬酰胺,表明该基因可能为OsWRG20的候选基因,与先前所报道的调控叶绿体发育的基因GRY79为等位基因。对该基因进行结构和功能分析,表明OsWRG20可能是调控水稻苗期幼叶生长发育的重要基因。与野生型相比,突变体的叶绿体基因内含子剪接效率降低,由此推断OsWRG20可能通过调控叶绿体RNA的剪接,参与调控水稻苗期叶绿体的发育。本研究结果为苗期水稻叶绿体发育研究提供了新的理论基础。
基金This work was supported by the Major Research Program on Technology of Agricultural Structure Adjustment (No. 05-01-05B)Jiangsu High Technology Program (No. BG2004301, BG2004304, and BG2005301).
文摘秋丰M来源于粳稻秋丰的自然白化转绿突变株。其主要特征为前三叶白化带绿,第四叶及以后叶片均为淡绿色,抽穗时,秋丰M的颖壳和前三叶一样仍出现带绿的白化现象。不同生长时期对野生型和突变型水稻叶片色素含量测定的结果与田间观察结果一致,秋丰M确实存在着一个叶色显著变化的过程。主要农艺性状的比较结果表明,秋丰与秋丰M除穗颈长和千粒重达到极显著差异外,其他农艺性状均无明显差异。遗传分析发现该突变性状受一对隐性核基因控制。以209株培矮64S×秋丰M F_2的隐性突变个体为定位群体,将突变基因定位在水稻第2染色体长臂上,位于 SSR 标记RM475和RM2-22之间,其遗传距离分别为17.3 cM和2.9 cM,并将该基因命名为gra_(t)。
基金This work was supported by Program for New Century Excellent Talents in University (No. NCET-04-0907) and Program for In-novative Research Team in University (No.IRT0453).
文摘在水稻品种宜香B中发现了一个白化转绿叶突变体,经过多代自交获得了稳定的白化转绿叶色突变体。该突变体在4叶期前叶色为黄绿色,之后逐渐变绿,从苗龄4周到12周,突变体/野生型叶绿素含量比值从34.5%逐渐升高到99.4%。遗传分析表明该突变受1对隐性核基因控制,暂命名为gra。利用微卫星标记将gra初步定位于第10染色体标记RM596和RM5620之间,进一步利用极端个体定位法把gra精细定位于标记RM25522和RM25535之间。gra基因距RM25522和RM25535标记的遗传距离均为0.05 c M,其物理距离约为136 kb。
