Although solar exposure is necessary for human health,phototoxicology induced by excessive UVB and UVA radiation,which involves sunburns,skin aging and even tu-morigenesis,has been widely researched.Sunscreen is one o...Although solar exposure is necessary for human health,phototoxicology induced by excessive UVB and UVA radiation,which involves sunburns,skin aging and even tu-morigenesis,has been widely researched.Sunscreen is one of the most important ways to protect skin from UV phototoxic damage.As well as inorganic and organic UV filters,some natural products or plant extracts with aromatic rings in their structures,such as flavonoids or polyphenols,can absorb UV to reduce sunburn,acting as a natu-ral UV filter;they also show antioxidant or/and anti-inflammatory activity.This could explain why,although there are no officially approval natural commercial sun-filters,more and more commercial sunscreen products containing plant extracts are avail-able on the market.Here we summarize articles focusing on natural UV filters from plant published in the last 6 years,selecting the most significant data in order to better understand the photoprotective activity of natural products and extracts from plants,including their major constituents and main biological effects,methods for evaluating UV radiation resistance,anti-UV radiation experimental models and anti-UV radiation mechanisms.展开更多
Extractive distillation(ED)and solvent-assisted pressure-swing distillation(SA-PSD)are both special distillation processes that perform good at separating pressure-insensitive azeotropes.However,few reported studies h...Extractive distillation(ED)and solvent-assisted pressure-swing distillation(SA-PSD)are both special distillation processes that perform good at separating pressure-insensitive azeotropes.However,few reported studies have compared the performance of the two processes.In this paper,ED processes with N-methylpyrrolidone(NMP)and dimethlac-etamide(DMCA)as entrainer,SA-PSD process with isopropyl-alcohol(IPA)as solvent and SA-PSD process with partial heat integration(PHI-PSD)are proposed to achieve high purity separation of a mixture of cyclohexane/2-butanol system.The optimal operating conditions of the processes are obtained after optimizing with NSGA-Ⅱ algorithm when total annual cost(TAC)and the entropy production of process are set as objectives.The optimal results show that the optimal PHI-PSD process has lower TAC by 28.7% and the lower entropy production by 39.5% than the optimal SA-PSD process while the ED process with NMP as entrainer has lower TAC by 50.9% and the lower entropy production by 56.1% than the optimal SA-PSD process.The optimal results show that the ED process with NMP as entrainer has the best economic and thermodynamic efficiency among the four proposed processes in this paper.展开更多
The acquisition of valuable design knowledge from massive fragmentary data is challenging for designers in conceptual product design.This study proposes a novel method for acquiring design knowledge by combining deep ...The acquisition of valuable design knowledge from massive fragmentary data is challenging for designers in conceptual product design.This study proposes a novel method for acquiring design knowledge by combining deep learning with knowledge graph.Specifically,the design knowledge acquisition method utilises the knowledge extraction model to extract design-related entities and relations from fragmentary data,and further constructs the knowledge graph to support design knowledge acquisition for conceptual product design.Moreover,the knowledge extraction model introduces ALBERT to solve memory limitation and communication overhead in the entity extraction module,and uses multi-granularity information to overcome segmentation errors and polysemy ambiguity in the relation extraction module.Experimental comparison verified the effectiveness and accuracy of the proposed knowledge extraction model.The case study demonstrated the feasibility of the knowledge graph construction with real fragmentary porcelain data and showed the capability to provide designers with interconnected and visualised design knowledge.展开更多
Gastric ulcer is a chronic disease featured with unexpected complications, including bleeding, stenosis and perforation, as well as a high incidence of recurrence. Clinical treatments for gastric ulcer have allowed th...Gastric ulcer is a chronic disease featured with unexpected complications, including bleeding, stenosis and perforation, as well as a high incidence of recurrence. Clinical treatments for gastric ulcer have allowed the rapid development of potent anti-ulcer drugs during the last several decades. Gastric ulcer healing is successful with conventional treatments including H2-receptor antagonists, and proton pump inhibitors(PPIs) have been essential for ulcer healing and prevention of complications. Additionally, Helicobacter pylori eradication therapy is effective in reducing ulcer recurrence and leads to physiological changes in the gastric mucosa which affect the ulcer healing process. However, in spite of these advancements, some patients have suf-fered from recurrence or intractability in spite of continuous anti-ulcer therapy. A new concept of the quality of ulcer healing(QOUH) was initiated that considers the reconstruction of the mucosal structure and its function for preventing ulcer recurrence. Although several gastroprotection provided these achievements of the QOUH, which PPI or other acid suppressants did not accomplish, we found that gastroprotection that originated from natural products, such as a newer formulation from either Artemisia or S-allyl cysteine from garlic, were very effective in the QOUH, as well as improving clinical symptoms with fewer side effects. In this review, we will introduce the importance of the QOUH in ulcer healing and the achievements from natural products.展开更多
The use of pan-ethnic-group products form knowledge primarily depends on a designer's subjective experience without user participation. The majority of studies primarily focus on the detection of the perceptual deman...The use of pan-ethnic-group products form knowledge primarily depends on a designer's subjective experience without user participation. The majority of studies primarily focus on the detection of the perceptual demands of consumers from the target product category. A pan-ethnic-group products form gene clustering method based on emotional semantic is constructed. Consumers' perceptual images of the pan-ethnic-group products are obtained by means of product form gene extraction and coding and computer aided product form clustering technology. A case of form gene clustering about the typical pan-ethnic-group products is investigated which indicates that the method is feasible. This paper opens up a new direction for the future development of product form design which improves the agility of product design process in the era of Industry 4.0.展开更多
Objective:To screen crude extracts of propolis,bee pollen and honey from four stingless bee species[Trigona incisa(T.incisa)],Timia apicalis,Trigona fuso-baltata and Trigona filscibasis)native to East Kalimantan.Indon...Objective:To screen crude extracts of propolis,bee pollen and honey from four stingless bee species[Trigona incisa(T.incisa)],Timia apicalis,Trigona fuso-baltata and Trigona filscibasis)native to East Kalimantan.Indonesia for cytotoxic activity against five human cancer cell lines(HepG2,SW620,ChaGo-1,KATO-Ⅲand BT474).Methods:All samples were extracted with methanol,and then subpartitioned with n-hexane and ethyl acetate.Each crude extract was screened at 20μg/mL for in vitro cytotoxicity against the cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Tn addition,four previously shown bioactive components from propolis(apigenin,cafieic acid phenyl ester,kaempferol and naringenin)and two chemotherapeutic drugs(doxorubicin and 5-fluorouracil)were used to evaluate the sensitivity of the cell lines.Results:Overall,crude extracts from propolis and honey had higher cytotoxic activities than bee pollen,but the activity was dependent upon the extraction solvent,bee species and cell line.Propolis extracts from T.incisa and Tarda apicalis showed the highest and lowest cytotoxic activity,respectively.Only the HepG2 cell line was broadly sensitive to the honey extracts.For pure compounds,doxorubicin was the most cytotoxic,the four propolis compounds the least,but the ChaGo-I cell line was sensitive to kaempferol at 10μg/mL and KATO-Ⅲwas sensitive to kaempferol and apigenin at 10μg/mL,.All pure compounds were effective against the BT474 cell line.Conclusions:Propolis from f,incisa and Trigona fusco-balteata contain an in vitro cytotoxic activity against human cancer cell lines.Further study is required,including the isolation and characterization of the active antiproliferative agent(s).展开更多
Bioactive natural products are a main source of new drugs,functional foods and food additives.The separation of bioactive natural products plays an important role in transformation and use of biomass.The isolation and...Bioactive natural products are a main source of new drugs,functional foods and food additives.The separation of bioactive natural products plays an important role in transformation and use of biomass.The isolation and purification of bioactive principle from a complex matrix is often inherent bottleneck for the utilization of natural products,so a series of extraction and separation techniques have been developed.This review covers recent advances in the separation of bioactive natural products with an emphasis on their solubility and diffusion coefficients,recent extraction techniques and isolation techniques.This overview of recent technological advances,discussion of pertinent problems and prospect of current methodologies in the separation of bioactive natural products may provide a driving force for development of novel separation techniques.展开更多
[Objectives]To explore the effect of the extract of Growgx yeast fermentation product on ultraviolet A(UVA)-induced photo-aging of human skin fibroblasts(HFF-1)and its specific mechanisms from the level of cell biolog...[Objectives]To explore the effect of the extract of Growgx yeast fermentation product on ultraviolet A(UVA)-induced photo-aging of human skin fibroblasts(HFF-1)and its specific mechanisms from the level of cell biology,and discussed the application of the Growgx yeast fermentation product extract for skin aging.[Methods]A photo-aging model of HFF-1 cells was induced by UVA radiation meter,and it was given different concentrations of Growgx for intervention.The proliferation activity of the HFF-1 cells was detected with MTT method,and their migration and invasion ability was measured by scratch test.The SOD,HYP and MDA levels were detected with corresponding kits.After oxygen infusion apparatus assisted facial skin administration,the repair of the skin was observed through the VISIA skin tester.[Results]The survival rate of HFF-1 cells was reduced significantly by UVA with an irradiation dose of 10.8 J/cm2(P<0.05),while Growgx significantly increased their survival rate(P<0.001)and sped up the repair of damaged cells.Growgx promoted the production of SOD(P<0.05),reduced the level of MDA(P<0.05),and increased the level of HYP(P<0.05).Growgx effectively inhibited UVA-induced photo-aging of HFF-1 cells.The mechanism may be related to accelerating cell damage repair,up-regulating SOD and HYP expression levels,and reducing MDA expression level.The clinical observation showed that Growgx effectively improved skin spots and pores,making the skin smoother and more delicate.[Conclusions]Growgx can effectively fight against photo-aging caused by ultraviolet rays,and can significantly improve skin wrinkles.展开更多
N,N,N′,N′-Tetraoctyl diglycolamide(TODGA)is one of the most promising extractants tailored for high-level liquid radioactive waste treatment during nuclear fuel reprocessing.Theγ-radiolysis of TODGA(0.2 mol/L)in n-...N,N,N′,N′-Tetraoctyl diglycolamide(TODGA)is one of the most promising extractants tailored for high-level liquid radioactive waste treatment during nuclear fuel reprocessing.Theγ-radiolysis of TODGA(0.2 mol/L)in n-dodecane(n DD)solution with and without pre-equilibrated 3.0 mol/L HNO_(3)was investigated using HPLC and UPLC-QTOF-MS and compared with theγ-radiolysis of neat TODGA in this study.With increased absorbed doses,the concentration of TODGA decreased exponentially for the studied systems.Moreover,pre-equilibration with HNO_(3)(3.0 mol/L)slightly influenced theγ-radiolysis of TODGA in n DD.Seven radiolytic products generated from the rupture of the C–C,C–O,and C–N bonds in TODGA were identified in the studied extraction system.The influence ofγ-radiation on TODGA/n DD for the extraction of Eu(Ⅲ)was evaluated using the first combination of extraction experiments and density functional theory(DFT)calculations,in which the complexations of Eu(Ⅲ)with TODGA and its radiolytic products were systematically compared.Based on the radiolysis kinetic model of TODGA,the slope curve of the distribution ratio of Eu(Ⅲ)(D_(Eu))and the absorbed dose,and fluorescence titration analysis,the empirical equation of the absorbed dose and D_(Eu)was obtained successfully.Below 300 kGy,the experimental D_(Eu)agreed well with the obtained empirical equation for TODGA/n DD.Conversely,at a high absorbed dose,the experimental D_(Eu)was higher than the theoretical D_(Eu)based on the empirical equation because the radiolytic products of TODGA with similar coordination structures still possessed partial complexation toward Eu(Ⅲ),which was confirmed by DFT calculations.This work provides a method to predict the extraction distribution ratio of an irradiated extractant system and to understand the complex extraction process.展开更多
β-glucans are bioactive compounds with a wide range of biological properties, including anticancer, anti-inflammatory, antioxidant, and immune-modulating properties. Due to their specific physical properties, such as...β-glucans are bioactive compounds with a wide range of biological properties, including anticancer, anti-inflammatory, antioxidant, and immune-modulating properties. Due to their specific physical properties, such as (in)solubility, viscosity, and gelation, β-glucans are increasingly being used in the food, pharmaceutical, and cosmetic industries. The purpose of this review is to provide an overview of the different types of β-glucans, their sources, especially Saccharomyces cerevisiae yeasts, and the methods of extraction, isolation, and purification of β-glucans, with the aim of optimizing these methods for the efficient production process. Moreover, the physico-chemical properties, modifications, current applications and future prospects of the use of β-glucans in food, medicines, cosmetics and other potential value-added products are summarized. The data presented indicate that β-glucans will play an increasingly important role in the sector of special-purpose food products as well as in other current and future areas.展开更多
The aim of this study was to develop and examine the morphology and distribution of mercury (Hg) in flue gas desulfurization (FGD) by-product.</span></span><span><span><span style="font...The aim of this study was to develop and examine the morphology and distribution of mercury (Hg) in flue gas desulfurization (FGD) by-product.</span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">Mercury in the coal of coal-fired power plants is concentrated in the by-products of desulfurization process, and it is widely used as an additive in cement, building materials and other industries. Due to the different stability of various forms of mercury in the environment, subsequent use of products containing desulfurization by-product additives will continue to be released into the environment, endangering human health. Therefore, it is very necessary to study the form and distribution of mercury in the by-products of desulfurization in coal-fired power plants to provide a theoretical basis for subsequent harmless treatment.</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">For content and morphology of mercury analysis, 1 sample of dry FGD ash and 6 samples of wet FGD gypsum were analyzed. The total 7 samples were extracted using a modification of sequential chemical extractions (SCE) method, which was employed for the partitioning Hg into four fractions: water soluble, acid soluble, H<sub>2</sub>O<sub>2</sub> soluble, and residual. The Hg analysis was done with United States Environmental Protection Agency (USEPA) method</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">7471B. Comparing with the wet FGD gypsums of coal-fired boilers, the total Hg content in the dry FGD by-product was as high as</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">1.22 mg/kg, while the total Hg content in the FGD gypsum is 0.23</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">-</span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">0.74 mg/kg, which was 2 times over the wet FGD gypsum. The concentration of water soluble Hg in the dry FGD by-product was the highest amount (0.72 mg/kg), accounting for 59.02% of the total mercury. While residual Hg content was 0.16 mg/kg, only about 13.11% of the total mercury. Mercury content in FGD gypsum was expressed in the form of <i></span><i><span style="font-family:Verdana;">ρ</span></i><span style="font-family:Verdana;"></i></span></span></span></span><span><span><i><span style="font-family:""> </span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">(residual Hg) ></span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"><i></span><i><span style="font-family:Verdana;">ρ</span></i><span style="font-family:Verdana;"></i></span></span></span></span><span><span><i><span style="font-family:""> </span></i></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">(H</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;">O</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;"> soluble Hg)</span></span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">></span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"><i></span><i><span style="font-family:Verdana;">ρ</span></i><span style="font-family:Verdana;"></i></span></span></span></span><span><span><i><span style="font-family:""> </span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">(water soluble Hg)</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">></span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"><i></span><i><span style="font-family:Verdana;">ρ</span></i><span style="font-family:Verdana;"></i></span></span></span></span><span><span><i><span style="font-family:""> </span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">(acid soluble Hg). The morphology and distribution of mercury in FGD by-products is supposed to be analyzed before utilization, and the impact of mercury on the environment should be considered.展开更多
建立一种基于美国官方分析化学师协会(Association of Official Analytical Chemists,AOAC)方法检测黑果枸杞及其制品中花青素含量的改进pH示差法。考察了黑果枸杞及其制品中花青素的最佳提取和检测条件,通过液相色谱-三重四级杆串联质...建立一种基于美国官方分析化学师协会(Association of Official Analytical Chemists,AOAC)方法检测黑果枸杞及其制品中花青素含量的改进pH示差法。考察了黑果枸杞及其制品中花青素的最佳提取和检测条件,通过液相色谱-三重四级杆串联质谱法鉴别出黑果枸杞中花青素的具体化学结构,并计算出混合花青素的平均摩尔质量。通过分光光度法测得混合花青素的平均摩尔消光系数,对改进后的pH示差法进行方法学验证和花青素的含量测定。结果显示,最佳提取和检测条件如下:黑果枸杞花青素提取溶剂为盐酸-80%(体积分数)乙醇(3∶97,体积比),料液比为1∶100(g∶mL),提取温度为50℃,提取时间为30 min,缓冲溶液稀释5倍后静置平衡20 min。液相色谱-三重四级杆串联质谱法鉴别黑果枸杞中主要以矮牵牛素类花青素为主(占97.96%),黑果枸杞特有的混合花青素平均摩尔质量为912.7 g/mol,平均摩尔消光系数为29591 L/(mol·cm)。pH示差法改进后能够满足方法学验证要求,固体样品和液体样品最低检出限分别为28.2 mg/100 g、0.282 mg/100 mL。方法改进后花青素提取增长率均大于20%,静置平衡20 min后单次检测结果精密度小于0.3%。以矮牵牛素类花青素代替矢车菊素-3-O-葡萄糖苷计算花青素含量平均提高了2.41倍,能真实地反映黑果枸杞及其制品中花青素的含量。展开更多
使用通过型固相萃取小柱PRi ME HLB处理水产品样品,建立了一种水产品中17种磺胺类药物的简单、快速的筛选分析方法。水产品样品经80%乙腈水溶液(含0.2%甲酸)提取,过PRi ME HLB固相萃取柱净化,浓缩后经C_(18)色谱柱梯度洗脱分离,超高效...使用通过型固相萃取小柱PRi ME HLB处理水产品样品,建立了一种水产品中17种磺胺类药物的简单、快速的筛选分析方法。水产品样品经80%乙腈水溶液(含0.2%甲酸)提取,过PRi ME HLB固相萃取柱净化,浓缩后经C_(18)色谱柱梯度洗脱分离,超高效液相色谱-三重四极杆质谱系统进行定量分析。结果表明,17种磺胺类药物在1.0~50.0 ng·mL^(-1)线性关系良好,相关系数R^(2)>0.99;该方法检出限为2μg·kg^(-1);添加浓度为10μg·kg^(-1)时方法回收率在71.3%~118.4%,RSD值均小于20%。展开更多
基金supported by Henan Provincial Department of Education(No.21B350001)Zhengzhou science and technology department(No.ZZSZX202109 and ZZSZX202108).
文摘Although solar exposure is necessary for human health,phototoxicology induced by excessive UVB and UVA radiation,which involves sunburns,skin aging and even tu-morigenesis,has been widely researched.Sunscreen is one of the most important ways to protect skin from UV phototoxic damage.As well as inorganic and organic UV filters,some natural products or plant extracts with aromatic rings in their structures,such as flavonoids or polyphenols,can absorb UV to reduce sunburn,acting as a natu-ral UV filter;they also show antioxidant or/and anti-inflammatory activity.This could explain why,although there are no officially approval natural commercial sun-filters,more and more commercial sunscreen products containing plant extracts are avail-able on the market.Here we summarize articles focusing on natural UV filters from plant published in the last 6 years,selecting the most significant data in order to better understand the photoprotective activity of natural products and extracts from plants,including their major constituents and main biological effects,methods for evaluating UV radiation resistance,anti-UV radiation experimental models and anti-UV radiation mechanisms.
基金supported by the National Natural Science Foundation of China(22178030,21878025,22078026)。
文摘Extractive distillation(ED)and solvent-assisted pressure-swing distillation(SA-PSD)are both special distillation processes that perform good at separating pressure-insensitive azeotropes.However,few reported studies have compared the performance of the two processes.In this paper,ED processes with N-methylpyrrolidone(NMP)and dimethlac-etamide(DMCA)as entrainer,SA-PSD process with isopropyl-alcohol(IPA)as solvent and SA-PSD process with partial heat integration(PHI-PSD)are proposed to achieve high purity separation of a mixture of cyclohexane/2-butanol system.The optimal operating conditions of the processes are obtained after optimizing with NSGA-Ⅱ algorithm when total annual cost(TAC)and the entropy production of process are set as objectives.The optimal results show that the optimal PHI-PSD process has lower TAC by 28.7% and the lower entropy production by 39.5% than the optimal SA-PSD process while the ED process with NMP as entrainer has lower TAC by 50.9% and the lower entropy production by 56.1% than the optimal SA-PSD process.The optimal results show that the ED process with NMP as entrainer has the best economic and thermodynamic efficiency among the four proposed processes in this paper.
基金This research is supported by the Chinese Special Projects of the National Key Research and Development Plan(2019YFB1405702).
文摘The acquisition of valuable design knowledge from massive fragmentary data is challenging for designers in conceptual product design.This study proposes a novel method for acquiring design knowledge by combining deep learning with knowledge graph.Specifically,the design knowledge acquisition method utilises the knowledge extraction model to extract design-related entities and relations from fragmentary data,and further constructs the knowledge graph to support design knowledge acquisition for conceptual product design.Moreover,the knowledge extraction model introduces ALBERT to solve memory limitation and communication overhead in the entity extraction module,and uses multi-granularity information to overcome segmentation errors and polysemy ambiguity in the relation extraction module.Experimental comparison verified the effectiveness and accuracy of the proposed knowledge extraction model.The case study demonstrated the feasibility of the knowledge graph construction with real fragmentary porcelain data and showed the capability to provide designers with interconnected and visualised design knowledge.
基金Supported by The National Center of Efficacy Evaluation for the Development of Health Products Targeting Digestive Disorders (NCEED) Grant (A102063) from the Ministry of Health and Welfare, South Korea
文摘Gastric ulcer is a chronic disease featured with unexpected complications, including bleeding, stenosis and perforation, as well as a high incidence of recurrence. Clinical treatments for gastric ulcer have allowed the rapid development of potent anti-ulcer drugs during the last several decades. Gastric ulcer healing is successful with conventional treatments including H2-receptor antagonists, and proton pump inhibitors(PPIs) have been essential for ulcer healing and prevention of complications. Additionally, Helicobacter pylori eradication therapy is effective in reducing ulcer recurrence and leads to physiological changes in the gastric mucosa which affect the ulcer healing process. However, in spite of these advancements, some patients have suf-fered from recurrence or intractability in spite of continuous anti-ulcer therapy. A new concept of the quality of ulcer healing(QOUH) was initiated that considers the reconstruction of the mucosal structure and its function for preventing ulcer recurrence. Although several gastroprotection provided these achievements of the QOUH, which PPI or other acid suppressants did not accomplish, we found that gastroprotection that originated from natural products, such as a newer formulation from either Artemisia or S-allyl cysteine from garlic, were very effective in the QOUH, as well as improving clinical symptoms with fewer side effects. In this review, we will introduce the importance of the QOUH in ulcer healing and the achievements from natural products.
基金Supported by National Key Technology R&D Program,China(Grant No.2015BAH21F01)National 111 Project,China(Grant No.B13044)
文摘The use of pan-ethnic-group products form knowledge primarily depends on a designer's subjective experience without user participation. The majority of studies primarily focus on the detection of the perceptual demands of consumers from the target product category. A pan-ethnic-group products form gene clustering method based on emotional semantic is constructed. Consumers' perceptual images of the pan-ethnic-group products are obtained by means of product form gene extraction and coding and computer aided product form clustering technology. A case of form gene clustering about the typical pan-ethnic-group products is investigated which indicates that the method is feasible. This paper opens up a new direction for the future development of product form design which improves the agility of product design process in the era of Industry 4.0.
基金Supported by East Kalimantan,Indonesia,the National Research Council of Thailand the Japan Society for the Promotion of Science and the Integrated Innovation Academic Center:HAC Chulalongkorn University Centenary Academic Development Project(Grant No.RES560530041)
文摘Objective:To screen crude extracts of propolis,bee pollen and honey from four stingless bee species[Trigona incisa(T.incisa)],Timia apicalis,Trigona fuso-baltata and Trigona filscibasis)native to East Kalimantan.Indonesia for cytotoxic activity against five human cancer cell lines(HepG2,SW620,ChaGo-1,KATO-Ⅲand BT474).Methods:All samples were extracted with methanol,and then subpartitioned with n-hexane and ethyl acetate.Each crude extract was screened at 20μg/mL for in vitro cytotoxicity against the cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Tn addition,four previously shown bioactive components from propolis(apigenin,cafieic acid phenyl ester,kaempferol and naringenin)and two chemotherapeutic drugs(doxorubicin and 5-fluorouracil)were used to evaluate the sensitivity of the cell lines.Results:Overall,crude extracts from propolis and honey had higher cytotoxic activities than bee pollen,but the activity was dependent upon the extraction solvent,bee species and cell line.Propolis extracts from T.incisa and Tarda apicalis showed the highest and lowest cytotoxic activity,respectively.Only the HepG2 cell line was broadly sensitive to the honey extracts.For pure compounds,doxorubicin was the most cytotoxic,the four propolis compounds the least,but the ChaGo-I cell line was sensitive to kaempferol at 10μg/mL and KATO-Ⅲwas sensitive to kaempferol and apigenin at 10μg/mL,.All pure compounds were effective against the BT474 cell line.Conclusions:Propolis from f,incisa and Trigona fusco-balteata contain an in vitro cytotoxic activity against human cancer cell lines.Further study is required,including the isolation and characterization of the active antiproliferative agent(s).
基金Supported by the National'Natural Science Foundation of China (20936005, 21076175 and 21076178), the National High Technology Research and Development Program of China (2012AA040211), and the Program for Zhejiang Leading Team of S&T Innovation (2011R50002).
文摘Bioactive natural products are a main source of new drugs,functional foods and food additives.The separation of bioactive natural products plays an important role in transformation and use of biomass.The isolation and purification of bioactive principle from a complex matrix is often inherent bottleneck for the utilization of natural products,so a series of extraction and separation techniques have been developed.This review covers recent advances in the separation of bioactive natural products with an emphasis on their solubility and diffusion coefficients,recent extraction techniques and isolation techniques.This overview of recent technological advances,discussion of pertinent problems and prospect of current methodologies in the separation of bioactive natural products may provide a driving force for development of novel separation techniques.
基金Supported by General Program of Guangxi Natural Science Foundation(2018GXNSFAA138098).
文摘[Objectives]To explore the effect of the extract of Growgx yeast fermentation product on ultraviolet A(UVA)-induced photo-aging of human skin fibroblasts(HFF-1)and its specific mechanisms from the level of cell biology,and discussed the application of the Growgx yeast fermentation product extract for skin aging.[Methods]A photo-aging model of HFF-1 cells was induced by UVA radiation meter,and it was given different concentrations of Growgx for intervention.The proliferation activity of the HFF-1 cells was detected with MTT method,and their migration and invasion ability was measured by scratch test.The SOD,HYP and MDA levels were detected with corresponding kits.After oxygen infusion apparatus assisted facial skin administration,the repair of the skin was observed through the VISIA skin tester.[Results]The survival rate of HFF-1 cells was reduced significantly by UVA with an irradiation dose of 10.8 J/cm2(P<0.05),while Growgx significantly increased their survival rate(P<0.001)and sped up the repair of damaged cells.Growgx promoted the production of SOD(P<0.05),reduced the level of MDA(P<0.05),and increased the level of HYP(P<0.05).Growgx effectively inhibited UVA-induced photo-aging of HFF-1 cells.The mechanism may be related to accelerating cell damage repair,up-regulating SOD and HYP expression levels,and reducing MDA expression level.The clinical observation showed that Growgx effectively improved skin spots and pores,making the skin smoother and more delicate.[Conclusions]Growgx can effectively fight against photo-aging caused by ultraviolet rays,and can significantly improve skin wrinkles.
文摘N,N,N′,N′-Tetraoctyl diglycolamide(TODGA)is one of the most promising extractants tailored for high-level liquid radioactive waste treatment during nuclear fuel reprocessing.Theγ-radiolysis of TODGA(0.2 mol/L)in n-dodecane(n DD)solution with and without pre-equilibrated 3.0 mol/L HNO_(3)was investigated using HPLC and UPLC-QTOF-MS and compared with theγ-radiolysis of neat TODGA in this study.With increased absorbed doses,the concentration of TODGA decreased exponentially for the studied systems.Moreover,pre-equilibration with HNO_(3)(3.0 mol/L)slightly influenced theγ-radiolysis of TODGA in n DD.Seven radiolytic products generated from the rupture of the C–C,C–O,and C–N bonds in TODGA were identified in the studied extraction system.The influence ofγ-radiation on TODGA/n DD for the extraction of Eu(Ⅲ)was evaluated using the first combination of extraction experiments and density functional theory(DFT)calculations,in which the complexations of Eu(Ⅲ)with TODGA and its radiolytic products were systematically compared.Based on the radiolysis kinetic model of TODGA,the slope curve of the distribution ratio of Eu(Ⅲ)(D_(Eu))and the absorbed dose,and fluorescence titration analysis,the empirical equation of the absorbed dose and D_(Eu)was obtained successfully.Below 300 kGy,the experimental D_(Eu)agreed well with the obtained empirical equation for TODGA/n DD.Conversely,at a high absorbed dose,the experimental D_(Eu)was higher than the theoretical D_(Eu)based on the empirical equation because the radiolytic products of TODGA with similar coordination structures still possessed partial complexation toward Eu(Ⅲ),which was confirmed by DFT calculations.This work provides a method to predict the extraction distribution ratio of an irradiated extractant system and to understand the complex extraction process.
文摘β-glucans are bioactive compounds with a wide range of biological properties, including anticancer, anti-inflammatory, antioxidant, and immune-modulating properties. Due to their specific physical properties, such as (in)solubility, viscosity, and gelation, β-glucans are increasingly being used in the food, pharmaceutical, and cosmetic industries. The purpose of this review is to provide an overview of the different types of β-glucans, their sources, especially Saccharomyces cerevisiae yeasts, and the methods of extraction, isolation, and purification of β-glucans, with the aim of optimizing these methods for the efficient production process. Moreover, the physico-chemical properties, modifications, current applications and future prospects of the use of β-glucans in food, medicines, cosmetics and other potential value-added products are summarized. The data presented indicate that β-glucans will play an increasingly important role in the sector of special-purpose food products as well as in other current and future areas.
文摘The aim of this study was to develop and examine the morphology and distribution of mercury (Hg) in flue gas desulfurization (FGD) by-product.</span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">Mercury in the coal of coal-fired power plants is concentrated in the by-products of desulfurization process, and it is widely used as an additive in cement, building materials and other industries. Due to the different stability of various forms of mercury in the environment, subsequent use of products containing desulfurization by-product additives will continue to be released into the environment, endangering human health. Therefore, it is very necessary to study the form and distribution of mercury in the by-products of desulfurization in coal-fired power plants to provide a theoretical basis for subsequent harmless treatment.</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">For content and morphology of mercury analysis, 1 sample of dry FGD ash and 6 samples of wet FGD gypsum were analyzed. The total 7 samples were extracted using a modification of sequential chemical extractions (SCE) method, which was employed for the partitioning Hg into four fractions: water soluble, acid soluble, H<sub>2</sub>O<sub>2</sub> soluble, and residual. The Hg analysis was done with United States Environmental Protection Agency (USEPA) method</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">7471B. Comparing with the wet FGD gypsums of coal-fired boilers, the total Hg content in the dry FGD by-product was as high as</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">1.22 mg/kg, while the total Hg content in the FGD gypsum is 0.23</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">-</span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">0.74 mg/kg, which was 2 times over the wet FGD gypsum. The concentration of water soluble Hg in the dry FGD by-product was the highest amount (0.72 mg/kg), accounting for 59.02% of the total mercury. While residual Hg content was 0.16 mg/kg, only about 13.11% of the total mercury. Mercury content in FGD gypsum was expressed in the form of <i></span><i><span style="font-family:Verdana;">ρ</span></i><span style="font-family:Verdana;"></i></span></span></span></span><span><span><i><span style="font-family:""> </span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">(residual Hg) ></span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"><i></span><i><span style="font-family:Verdana;">ρ</span></i><span style="font-family:Verdana;"></i></span></span></span></span><span><span><i><span style="font-family:""> </span></i></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">(H</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;">O</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;"> soluble Hg)</span></span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">></span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"><i></span><i><span style="font-family:Verdana;">ρ</span></i><span style="font-family:Verdana;"></i></span></span></span></span><span><span><i><span style="font-family:""> </span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">(water soluble Hg)</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">></span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"><i></span><i><span style="font-family:Verdana;">ρ</span></i><span style="font-family:Verdana;"></i></span></span></span></span><span><span><i><span style="font-family:""> </span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">(acid soluble Hg). The morphology and distribution of mercury in FGD by-products is supposed to be analyzed before utilization, and the impact of mercury on the environment should be considered.
文摘使用通过型固相萃取小柱PRi ME HLB处理水产品样品,建立了一种水产品中17种磺胺类药物的简单、快速的筛选分析方法。水产品样品经80%乙腈水溶液(含0.2%甲酸)提取,过PRi ME HLB固相萃取柱净化,浓缩后经C_(18)色谱柱梯度洗脱分离,超高效液相色谱-三重四极杆质谱系统进行定量分析。结果表明,17种磺胺类药物在1.0~50.0 ng·mL^(-1)线性关系良好,相关系数R^(2)>0.99;该方法检出限为2μg·kg^(-1);添加浓度为10μg·kg^(-1)时方法回收率在71.3%~118.4%,RSD值均小于20%。