Impact of Alcian blue and periodic acid Schiff expression on the prognosis of gastric signet ring cell carcinoma

BACKGROUND The Alcian blue(AB)and periodic acid Schiff(PAS)stains are representative mucus markers in gastric signet ring cell carcinoma(SRCC).They are low-cost special staining methods used to detect acidic mucus and neutral mucus,respectively.However,the clinical importance of the special combined AB and PAS stain is unclear.AIM To investigate AB expression,PAS expression and the AB-to-PAS(A/P)ratio in gastric SRCC patients and to assess patient prognosis.METHODS Paraffin-embedded sections from 83 patients with gastric SRCC were stained with AB and PAS,and signet ring cell positivity was assessed quantitatively.Immuno-histochemical staining for Ki67,protein 53(P53)and human epidermal growth factor receptor 2(HER2)was performed simultaneously.The cancer-specific survival(CSS)rate was estimated via Kaplan-Meier analysis.Cox proportional hazards models were used for univariate and multivariate survival analyses.RESULTS Kaplan-Meier survival analysis revealed that the 3-year CSS rate was significantly greater in the high-PAS-expression subgroup than in the low-PAS-expression subgroup(P<0.001).The 3-year CSS rate in the A/P≤0.5 group was significantly greater than that in the A/P>0.5 group(P=0.042).Univariate Cox regression analysis revealed that the factors affecting prognosis included tumor diameter,lymph node metastasis,vessel carcinoma embolus,tumor stage,the A/P ratio and the expression of Ki67,P53 and the PAS.Cox multivariate regression analysis confirmed that low PAS expression[hazard ratio(HR)=3.809,95%confidence interval(CI):1.563-9.283,P=0.003]and large tumor diameter(HR=2.761,95%CI:1.086-7.020,P=0.033)were independent risk factors for poor prognosis.CONCLUSION A/P>0.5 is potentially a risk factor for prognosis,and low PAS expression is an independent risk factor in the prognosis of gastric SRCC.PAS expression and the A/P ratio could help in predicting the clinical prognosis of patients with SRCC.

PAS、AB染色法在软骨蛋白黏多糖检测中的运用

[目的 ] 探讨过碘酸雪夫氏反应PAS染色 (PeriodicAcidSchiff)及阿利辛兰AB染色 (AlcianBlue)组织化学分析法在检测软骨组织蛋白黏多糖含量中的运用价值。 [方法 ] 以原发性退行性骨关节炎动物模型的软骨为检测标本 ,分别采用PAS和AB染色法 ,观察软骨组织中蛋白黏多糖的含量。 [结果 ] PAS染色法中软骨组织基质中的中性黏多糖呈紫红色 ,软骨细胞内为无色。AB法中软骨细胞周围的酸性黏多糖呈深蓝色 ,基质呈淡蓝色。此外 ,随着动物模型关节炎程度的不断加剧 ,即软骨组织中蛋白黏多糖丢失的不断增加 ,标本中的紫红色和深蓝色也相应减退 ,直至消失。 [结论 ] PAS染色法和AB染色法能清楚地区分软骨基质中的中性黏多糖和细胞囊内的酸性黏多糖 ,并能对它们的改变进行量化分析。可作为软骨组织中黏多糖检测的有效方法。

成年牦牛汗腺和皮脂腺的组织学特征

目的探讨青海健康成年牦牛汗腺和皮脂腺的组织学特征。方法从10头健康成年牦牛背部、肋部和腹部皮肤取样,运用HE、过碘酸-雪夫(PAS)和阿利新蓝-过碘酸-雪夫(AB-PAS)染色法,对汗腺和皮脂腺组织学结构进行观察与分析。结果青海成年牦牛背部、肋部和腹部皮肤的汗腺为单管状腺,属于顶浆分泌型腺体,其分泌物为中性和酸性混合液,腺细胞胞质中含有糖原。皮脂腺呈葡萄状、多叶且分支的泡状腺,属于全浆分泌型腺体,其分泌物为中性黏液,腺细胞胞质中含有少量糖原。汗腺的密度、直径、长度和体积从背部到腹部呈递减趋势,但距离皮肤表面的深度呈递增趋势,且部位间差异显著(P<0.05)。皮脂腺的密度、导管部直径和长度、分泌部直径和长度及距离皮肤表面的深度从背部到腹部呈递减趋势,且部位间差异显著(P<0.05)。结论青海成年牦牛背部、肋部和腹部皮肤的汗腺和皮脂腺形态学结构相似,但其密度、大小和深度差异显著,且从背部到腹部存在渐变趋势。与其他牛类相比,牦牛汗腺和皮脂腺的密度高,但前者的分泌功能不发达而后者发达,这可能与其为了适应高原高寒环境有关。

AB-PAS染色联合免疫组织化学检查在卵巢黏液性肿瘤中的应用研究

目的探讨阿利新蓝-过碘酸-雪夫(alcian blue/periodic acid-Schiff,AB-PAS)染色联合免疫组织化学检查在卵巢黏液性肿瘤中的应用研究。方法收集作者医院2018-08/2020-12月诊治的72例原发性卵巢黏液性肿瘤和12例转移性卵巢黏液性肿瘤患者为研究对象。免疫组织化学方法检测细胞角蛋白7(cytokeratin 7,CK7)、细胞角蛋白20(cytokeratin 20,CK20)、尾型同源核转录因子2(caudal-related homeobox transcription factor 2,CDX2)、富含AT序列特异性结合蛋白2(special AT-rich sequence-binding protein 2,SATB2)在肿瘤中的表达。AB-PAS染色检测肿瘤黏液的表达。结果原发性卵巢黏液性肿瘤患者年龄(42.7±15.1)岁小于转移性卵巢黏液性肿瘤患者年龄(51.1±8.6)岁,肿瘤最大直径(14.1±7.3)cm大于转移性卵巢黏液性肿瘤直径(10.3±5.2)cm(P均<0.05);原发性卵巢黏液性肿瘤发生部位[左侧39例(52.4%)、右侧33例(45.8%)]与转移性卵巢黏液性肿瘤[左侧1例(8.3%)、右侧5例(41.7%)、双侧6例(50.0%)]比较差异有统计学意义(P<0.05)。CK7在原发性卵巢黏液性肿瘤中的表达率(93.1%)显著高于转移性卵巢黏液性肿瘤(25.0%)(P<0.01)。CK20、CDX2、SATB2在原发性卵巢黏液性肿瘤中的表达率(27.8%、30.6%、1.4%)显著低于转移性卵巢黏液性肿瘤(100.0%、91.7%、91.7%)(P均<0.01)。AB-PAS染色在原发性卵巢黏液性肿瘤与转移性卵巢黏液性肿瘤表达差异具有统计学意义(P<0.01)。原发性卵巢黏液性肿瘤主要表达红色,转移性卵巢黏液性肿瘤显著表达蓝色。结论免疫组织化学联合AB-PAS染色的运用对于明确卵巢黏液性肿瘤的诊断具有支持和辅助意义。