Harvesting photosynthetic electrons (PEs) from plant or algal cells can be a highly efficient and environmentally friendly way of generating renewable energy. Recent work on nanoelectrode insertion into algal cells ...Harvesting photosynthetic electrons (PEs) from plant or algal cells can be a highly efficient and environmentally friendly way of generating renewable energy. Recent work on nanoelectrode insertion into algal cells has demonstrated the possibility to directly extract PEs from living algal cells with high efficiencies. However, the instability of the inserted cells limits the practicality of this technology. Here, the impact of nanoelectrode insertion on intracellular extraction of PEs is characterized with the goal of stabilizing algal cells after nanoelectrode insertion. Using nanoelectrodes 〈 500 nm in diameter, algal cells remained stable for over one week after insertion and continued to provide PEs through direct extraction by the inserted nanoelectrodes. After nanoelectrode insertion, a photosynthetic current density of 6 mA.cm-2, which is several fold higher than the current densities attained using approaches based on isolated thylakoid membranes or photosystem I complexes, was observed in the dark and during illumination at various light intensities.展开更多
Nano-sized titanium dioxide (nano-TiO2) has wide industrial applications and therefore considerable chances of exposure are created for human beings and ecosystems. To better understand the interactions between nano...Nano-sized titanium dioxide (nano-TiO2) has wide industrial applications and therefore considerable chances of exposure are created for human beings and ecosystems. To better understand the interactions between nano-TiO2 and aquatic organisms, we first studied TiO2 uptake by algae exemplified by Pseudokirchneriella subcapitata. P. subcapitata were exposed to nano-TiO2 in a series of concentrations and at various pH. TiO2 uptake was quantified using a sedimentation curve analysis technique. After exposure of algae to TiO2, the variation of zeta potential was measured and the morphology of algae-TiO2 aggregate was observed with scanning electron microscopy and the optical microscopy. The steady-state TiO2 uptake was found to be pH-dependent and the isotherms can be described well by Freundlich model. TiO2 deposited on algal surfaces causes the shift of pHzpc of TiO2-covered algae from that of algae toward that of TiO2. The attraction between TiO2-covered algal cells induces the agglomeration of algae and TiO2 and thus the formation of algae-TiO2 aggregates in the size of 12 to 50 μm. The 2-D fractal dimension of the aggregates is pH- dependent and ranges from 1.31 to 1.67. The theoretical analysis of the Gibbs energy of interaction indicates that both TiO2 uptake by algae and the formation of algae-TiO2 aggregate are influenced by the interaction between TiO2 particles.展开更多
Blue mussels, Mytilus edulis , form dense beds of both commercial and ecological importance, and many attempts have been made to determine their filtration rate. The total time in which mussels actually utilise their ...Blue mussels, Mytilus edulis , form dense beds of both commercial and ecological importance, and many attempts have been made to determine their filtration rate. The total time in which mussels actually utilise their filtration capacity in nature varies greatly, making in situ methods for filtration rate measurements relevant. Further, it is being debated to what extend filtration rates measured in the laboratory using cultivated algal cells may apply for mussels in nature. In the present study, we have used an open-top chamber setup in order to allow repeated in situ filtration rate measurements of M. edulis using ambient natural phytoplankton and free-living bacteria. We found that the in situ measured filtration rates are comparable to filtration rates obtained in laboratory studies using different methods and controlled diets of cultivated algal cells. Further, we found that the retention efficiency of free-living bacteria was between 22.2% and 29.9%, in good agreement with values from laboratory studies. Our findings support the assumption that mussels in nature tend to use their filtration capacity when the phytoplankton concentration is above a certain lower trigger level.展开更多
文摘Harvesting photosynthetic electrons (PEs) from plant or algal cells can be a highly efficient and environmentally friendly way of generating renewable energy. Recent work on nanoelectrode insertion into algal cells has demonstrated the possibility to directly extract PEs from living algal cells with high efficiencies. However, the instability of the inserted cells limits the practicality of this technology. Here, the impact of nanoelectrode insertion on intracellular extraction of PEs is characterized with the goal of stabilizing algal cells after nanoelectrode insertion. Using nanoelectrodes 〈 500 nm in diameter, algal cells remained stable for over one week after insertion and continued to provide PEs through direct extraction by the inserted nanoelectrodes. After nanoelectrode insertion, a photosynthetic current density of 6 mA.cm-2, which is several fold higher than the current densities attained using approaches based on isolated thylakoid membranes or photosystem I complexes, was observed in the dark and during illumination at various light intensities.
文摘Nano-sized titanium dioxide (nano-TiO2) has wide industrial applications and therefore considerable chances of exposure are created for human beings and ecosystems. To better understand the interactions between nano-TiO2 and aquatic organisms, we first studied TiO2 uptake by algae exemplified by Pseudokirchneriella subcapitata. P. subcapitata were exposed to nano-TiO2 in a series of concentrations and at various pH. TiO2 uptake was quantified using a sedimentation curve analysis technique. After exposure of algae to TiO2, the variation of zeta potential was measured and the morphology of algae-TiO2 aggregate was observed with scanning electron microscopy and the optical microscopy. The steady-state TiO2 uptake was found to be pH-dependent and the isotherms can be described well by Freundlich model. TiO2 deposited on algal surfaces causes the shift of pHzpc of TiO2-covered algae from that of algae toward that of TiO2. The attraction between TiO2-covered algal cells induces the agglomeration of algae and TiO2 and thus the formation of algae-TiO2 aggregates in the size of 12 to 50 μm. The 2-D fractal dimension of the aggregates is pH- dependent and ranges from 1.31 to 1.67. The theoretical analysis of the Gibbs energy of interaction indicates that both TiO2 uptake by algae and the formation of algae-TiO2 aggregate are influenced by the interaction between TiO2 particles.
基金supported by a research grant(9278)from VILLUM FONDEN
文摘Blue mussels, Mytilus edulis , form dense beds of both commercial and ecological importance, and many attempts have been made to determine their filtration rate. The total time in which mussels actually utilise their filtration capacity in nature varies greatly, making in situ methods for filtration rate measurements relevant. Further, it is being debated to what extend filtration rates measured in the laboratory using cultivated algal cells may apply for mussels in nature. In the present study, we have used an open-top chamber setup in order to allow repeated in situ filtration rate measurements of M. edulis using ambient natural phytoplankton and free-living bacteria. We found that the in situ measured filtration rates are comparable to filtration rates obtained in laboratory studies using different methods and controlled diets of cultivated algal cells. Further, we found that the retention efficiency of free-living bacteria was between 22.2% and 29.9%, in good agreement with values from laboratory studies. Our findings support the assumption that mussels in nature tend to use their filtration capacity when the phytoplankton concentration is above a certain lower trigger level.