The purpose of this work is to study the co-cultivation of Chlorella sp. and wastewater wild algae under different cultivation conditions （i.e. CO2, light intensity, cultivation time, and inoculation ratio） for enha...The purpose of this work is to study the co-cultivation of Chlorella sp. and wastewater wild algae under different cultivation conditions （i.e. CO2, light intensity, cultivation time, and inoculation ratio） for enhanced algal biomass and lipid productivity in wastewater medium using Response SuHhce Methodology （RSM）. The results show that mixed cultures ofd7llorella sp. and wastewater wild algae increase biomass and lipid yield. Additionally, findings indicate that CO2, light intensity and cultivation time significantly affect algal productivity. Furthcnnore, CO2 concentration and light intensity, and CO2 concentration and algal composition, have an interactive effect on biomass productivity. Under dii＂ferent cultivation conditions, the response of algal biomass, cell count, and lipid productlvlty ranges from2,5 to 10.2 mg/mL 1.1 × 10 to 8.2 × 10 cells/mL and 1.1 × 10^6 to 6.8 × 10^12 total fluorescent units/mL, respectively× The optimum conditions tbr simt, ltaneot, s biomass and lipid accumulation are 3.6% of CO2 （v/v）, 160 μmol/m^2/s of light intensity, 1×6/2.4 of inoculation ratio （wastewater-algae/Chlorella）, and 8.3 days of cultivation time. The optimal productivity is 9,8 （g/L） for dry biomass, 8.6 E ＋ 08 （cells/mL） for cell count, and 6.8 E ＋ 12 （Total FL units per mL） fbr lipid yield, achieving up to four times, eight times, and seven times higher productivity compared to non- optimized conditions. Provided is a supportive methodology to improve mixed algal culture for hioenergy feedstock generation and to optimize cultivation conditions in complex wastewater environments. This work is an important step tbrward in the development of sustainable large-scale algae cultivation for cost-efficient generation of biofuel.展开更多
文摘The purpose of this work is to study the co-cultivation of Chlorella sp. and wastewater wild algae under different cultivation conditions （i.e. CO2, light intensity, cultivation time, and inoculation ratio） for enhanced algal biomass and lipid productivity in wastewater medium using Response SuHhce Methodology （RSM）. The results show that mixed cultures ofd7llorella sp. and wastewater wild algae increase biomass and lipid yield. Additionally, findings indicate that CO2, light intensity and cultivation time significantly affect algal productivity. Furthcnnore, CO2 concentration and light intensity, and CO2 concentration and algal composition, have an interactive effect on biomass productivity. Under dii＂ferent cultivation conditions, the response of algal biomass, cell count, and lipid productlvlty ranges from2,5 to 10.2 mg/mL 1.1 × 10 to 8.2 × 10 cells/mL and 1.1 × 10^6 to 6.8 × 10^12 total fluorescent units/mL, respectively× The optimum conditions tbr simt, ltaneot, s biomass and lipid accumulation are 3.6% of CO2 （v/v）, 160 μmol/m^2/s of light intensity, 1×6/2.4 of inoculation ratio （wastewater-algae/Chlorella）, and 8.3 days of cultivation time. The optimal productivity is 9,8 （g/L） for dry biomass, 8.6 E ＋ 08 （cells/mL） for cell count, and 6.8 E ＋ 12 （Total FL units per mL） fbr lipid yield, achieving up to four times, eight times, and seven times higher productivity compared to non- optimized conditions. Provided is a supportive methodology to improve mixed algal culture for hioenergy feedstock generation and to optimize cultivation conditions in complex wastewater environments. This work is an important step tbrward in the development of sustainable large-scale algae cultivation for cost-efficient generation of biofuel.
